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Gene information
General information
TC007074 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 10084109..10090969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007074
    iBeetle screen iB_01121
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    remarks: entire repetition development delayed, later than schedule, hours 60 adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2


    remarks: entire repetition development delayed, later than schedule, hours 60
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007074 RA
      MDVSRTLFL QLPKEKTID IEFDNVTYS VPEGRKGRK 
      QIVKGVCGK FKAGELTAI MGPSGAGKT SLLNILTGF 
      QTVGMHGVI KSTHDKEVR FGAQQYKKQ SCYILQDDQ 
      LAPLFTVAE IMNMAADLK LGHGISSKR KCMLIDDIL 
      DTLGLSTAK DTRCQRLSG GQKKRLSIA LELIDNPPI 
      MFLDEPTTG LDSSSSAQC ISMLKELAR GGRTIICTI 
      HQPSATLYE MFDHVYVMA EGKCIYQGA SQNTVTYLS 
      SIGFNCPQY HNPADYLLE VANGEYGNF TDFLAKAAT 
      ENQWRTAVT SDKLENKIN EKTENTVEN AKTYQHTQP 
      PSELFKFYI LVKRSFLQM YRDWTISHL KILLHFLVG 
      IVLGLNYYQ SGNDGSKTI SNVGFFIVS SVYLTYTSL 
      MPAVLKFPS ELAVLKKER FNNWYKLKT YYAAFLMSD 
      VPMQIIFCM SYISTSYFL SDQPSESFR FFMVVGILI 
      LLSLTASSL GLVIGTLVN PINGTFFGA IITAVMLCF 
      AGFLILFTH MSKPMYMMT YVSFLAYSY EGLVQAVYG 
      FNRPSIPCP EEVEYCHLK VPELILREL GMTNNTYWT 
      DVAFLFGNF LLLRIVAFC TLKRRLRTG
    CDS sequence
    • >TC007074 RA
      atggacgta agcagaacg ctttttttg caactaccg 
      aaagaaaaa accatcgat atcgaattc gataatgtc 
      acatattcg gttccggaa ggacgaaaa ggaaggaag 
      caaatcgtc aaaggtgtg tgtggcaag ttcaaagcg 
      ggcgagttg acggccatt atgggacct tccggagcc 
      ggaaaaaca tccctactt aacatctta accggattt 
      cagaccgtg ggaatgcac ggtgtgata aaaagcacc 
      cacgacaaa gaggttcgt ttcggagct cagcaatac 
      aaaaagcag tcctgttac atcctgcag gacgaccaa 
      ctggcgccc cttttcaca gtcgccgag ataatgaac 
      atggctgca gacttgaaa ctaggacac ggtatatca 
      tcgaaaagg aaatgcatg ctgatcgat gatattttg 
      gatactttg ggtctgtcg acggcaaag gacacgcgt 
      tgtcaacga ttatctggt ggccagaaa aaacgtctc 
      tcgatcgct cttgaactc atcgacaat ccgcccatt 
      atgttcttg gacgaaccg accacaggt ttggacagc 
      tcgtcctcg gcacaatgt attagcatg ttgaaagaa 
      cttgcaaga ggcggaaga acaattatt tgcacgatt 
      catcaaccg agtgccacg ctatacgaa atgtttgat 
      cacgtctac gtcatggcc gaaggcaaa tgcatttat 
      cagggggcc agccaaaac acggtcact tatttatcg 
      tcaattggg ttcaactgc ccccagtat cacaacccc 
      gcagattat ctattggaa gttgctaat ggcgaatat 
      ggtaatttc accgatttt ttggcgaaa gcagcgacc 
      gaaaaccag tggagaaca gcagtgacc tcggataaa 
      ctggagaac aaaataaac gaaaagacg gagaacaca 
      gttgaaaat gcaaaaact tatcagcac acgcaacca 
      ccctctgag ttgtttaaa ttttacatt ctagtcaaa 
      cgatcgttt ttgcaaatg tacagggac tggacgatt 
      tctcatttg aaaatcctg ctgcatttc ctcgtagga 
      atcgtccta gggttaaat tattaccaa tcgggaaat 
      gacgggtcg aagacgatc agtaatgta ggtttcttc 
      atcgtaagt tcggtttat ttgacgtac acatctcta 
      atgcccgcc gtactcaaa tttccatct gaactggca 
      gtattaaaa aaagaacgg ttcaataac tggtacaaa 
      ctcaaaaca tattacgct gcattttta atgtcagac 
      gtaccgatg caaataatt ttttgcatg tcgtacatc 
      tctacgtcg tactttttg agcgaccag ccctcggaa 
      agtttccga ttttttatg gtggtcggt atattaatt 
      ctactctca ctgacagcg tccagtctt ggattagtc 
      atcgggact ttggtaaac ccaattaac ggaacattc 
      tttggcgct attataacc gccgtcatg ttatgtttt 
      gccggtttt ttgattttg ttcactcac atgtcgaaa 
      ccaatgtac atgatgacc tatgtgtcg tttttggca 
      tactcttac gaaggcttg gtccaagca gtttacggt 
      tttaaccga ccctcaatt ccgtgtcct gaggaggtc 
      gagtattgc catttgaaa gtgcctgag ctcattttg 
      agagagttg ggaatgaca aacaatact tattggacc 
      gatgttgct tttcttttt ggcaatttc ctcctactg 
      aggatagtc gcgttttgc actttgaaa cggcggctg 
      aggaccggt tga
    mRNA sequence
    • >TC007074 RA
      cgatatttg ataagccat cctacatac aattagtgt 
      cattccttg tcgatacgc gatggttga aattccaaa 
      attccgtca aatcgcgac cacctgttc ccccgtatt 
      ttaatccgc gttggtcgt tgcttgggc atggtttag 
      ttcgcgacg taccatcgt ttccaccac acacgtcac 
      gaatttcac gaaaagcga gcccgggtg tgctgctag 
      cgccaaaaa atttcgact ttaagtgcg tgaatgagt 
      gcataataa ttttcgaaa atggacgta agcagaacg 
      ctttttttg caactaccg aaagaaaaa accatcgat 
      atcgaattc gataatgtc acatattcg gttccggaa 
      ggacgaaaa ggaaggaag caaatcgtc aaaggtgtg 
      tgtggcaag ttcaaagcg ggcgagttg acggccatt 
      atgggacct tccggagcc ggaaaaaca tccctactt 
      aacatctta accggattt cagaccgtg ggaatgcac 
      ggtgtgata aaaagcacc cacgacaaa gaggttcgt 
      ttcggagct cagcaatac aaaaagcag tcctgttac 
      atcctgcag gacgaccaa ctggcgccc cttttcaca 
      gtcgccgag ataatgaac atggctgca gacttgaaa 
      ctaggacac ggtatatca tcgaaaagg aaatgcatg 
      ctgatcgat gatattttg gatactttg ggtctgtcg 
      acggcaaag gacacgcgt tgtcaacga ttatctggt 
      ggccagaaa aaacgtctc tcgatcgct cttgaactc 
      atcgacaat ccgcccatt atgttcttg gacgaaccg 
      accacaggt ttggacagc tcgtcctcg gcacaatgt 
      attagcatg ttgaaagaa cttgcaaga ggcggaaga 
      acaattatt tgcacgatt catcaaccg agtgccacg 
      ctatacgaa atgtttgat cacgtctac gtcatggcc 
      gaaggcaaa tgcatttat cagggggcc agccaaaac 
      acggtcact tatttatcg tcaattggg ttcaactgc 
      ccccagtat cacaacccc gcagattat ctattggaa 
      gttgctaat ggcgaatat ggtaatttc accgatttt 
      ttggcgaaa gcagcgacc gaaaaccag tggagaaca 
      gcagtgacc tcggataaa ctggagaac aaaataaac 
      gaaaagacg gagaacaca gttgaaaat gcaaaaact 
      tatcagcac acgcaacca ccctctgag ttgtttaaa 
      ttttacatt ctagtcaaa cgatcgttt ttgcaaatg 
      tacagggac tggacgatt tctcatttg aaaatcctg 
      ctgcatttc ctcgtagga atcgtccta gggttaaat 
      tattaccaa tcgggaaat gacgggtcg aagacgatc 
      agtaatgta ggtttcttc atcgtaagt tcggtttat 
      ttgacgtac acatctcta atgcccgcc gtactcaaa 
      tttccatct gaactggca gtattaaaa aaagaacgg 
      ttcaataac tggtacaaa ctcaaaaca tattacgct 
      gcattttta atgtcagac gtaccgatg caaataatt 
      ttttgcatg tcgtacatc tctacgtcg tactttttg 
      agcgaccag ccctcggaa agtttccga ttttttatg 
      gtggtcggt atattaatt ctactctca ctgacagcg 
      tccagtctt ggattagtc atcgggact ttggtaaac 
      ccaattaac ggaacattc tttggcgct attataacc 
      gccgtcatg ttatgtttt gccggtttt ttgattttg 
      ttcactcac atgtcgaaa ccaatgtac atgatgacc 
      tatgtgtcg tttttggca tactcttac gaaggcttg 
      gtccaagca gtttacggt tttaaccga ccctcaatt 
      ccgtgtcct gaggaggtc gagtattgc catttgaaa 
      gtgcctgag ctcattttg agagagttg ggaatgaca 
      aacaatact tattggacc gatgttgct tttcttttt 
      ggcaatttc ctcctactg aggatagtc gcgttttgc 
      actttgaaa cggcggctg aggaccggt tgatataat 
      ttttgtgat tatcttact aacattagg tgattttta 
      tgagagatt atttatatg gactagttt tgtaaatat 
      acaagtgtg gtgtgagat acaatactt gttttgtat 
      tatacaaag attctattt aataaaaaa ttactgtac 
      tg