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Gene information
General information
TC032936
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007070
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10115004..10116589
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032936
    iBeetle screen iB_01120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032936 RA
      MKGPTHPDG GYGWVVVFA SALTNFITL SLIQCFGLV 
      LKDTFAELN LSATQGSLI INLNGAFGL LTGPVTGVL 
      QKLFGCRRI AFFGGLLLT FGMVLTSFS RSFGSLVVT 
      YGLVTSLGM QMCESSQRL AINQYFKKK RSLVTGFIM 
      TAAGFGPIL IPQLIRVLL KNYSTNEVT LIYGGLCAH 
      ILIAGALLQ PIEWHSKEQ GTKTEKIEL KERNEIIIS 
      EEKFLSKIV DNLAKIFDL DLLKDPVFN NIIIGLALA 
      TFAEINFTL LVPFILNDF KLTTDQIAI FLSTLGISD 
      MISRFLGPY VGNYFTKPS RLMFVYSLV ILVIIKFTI 
      LCSKNFIFL LVIAFTLGI AKGIRKVYM WLVIPDYVP 
      LEKLPSAAG LDMLARGCC ILIGGPILG ALRDATGSY 
      VICIIVMNC VTITTILLW LIEGILTKY TKIKILHRN 
      T
    CDS sequence
    • >TC032936 RA
      atgaaaggg ccaacgcat cctgatgga ggctacggc 
      tgggtggtg gtcttcgcc agcgcgcta accaacttc 
      atcaccctc tctctaatc caatgtttc ggcctcgtc 
      ctcaaagac acgttcgcc gaattaaac ctgtcggca 
      actcaaggc tcgctcatc attaacttg aacggagct 
      ttcggtctt ctgaccgga ccagtgact ggagtttta 
      cagaaactg ttcggttgc cgaagaatc gcgtttttt 
      ggggggctt ttactcacg ttcggaatg gttctgacg 
      tctttctcc cgaagtttc gggtctctg gtcgtcact 
      tacggactc gtaacttca cttgggatg caaatgtgc 
      gaatcgagc caaaggctg gcgattaat cagtatttc 
      aaaaagaag aggagttta gtgacgggg tttataatg 
      acagcggct ggatttgga ccgattttg atcccgcag 
      ctgatccga gttttgctg aaaaattac tcgacgaat 
      gaggtgacg ctgatttat ggcgggttg tgtgcgcat 
      attctcata gcgggggcc cttttgcaa ccaatcgag 
      tggcactcg aaggaacaa gggacgaaa acggaaaaa 
      atcgagctc aaggaacgg aacgaaatt atcatttcc 
      gaggagaag ttcctaagc aaaattgtg gacaatctg 
      gcgaagatt ttcgatttg gatttgctc aaagacccg 
      gtgtttaat aacattatt ataggcctg gctttggcc 
      acgtttgcc gaaatcaat ttcactctc cttgtcccg 
      tttattcta aatgacttc aaattaacc acggatcaa 
      atcgcgatt tttctctcg actttaggg atttccgac 
      atgatctcg cgtttcctg ggcccctat gttggtaat 
      tattttacc aaaccttca aggctcatg tttgtctat 
      tctcttgta atcctagtt ataattaaa tttacaata 
      ctatgctcc aaaaacttc attttcctc cttgttatt 
      gcttttact ttgggtata gccaaagga attcgaaag 
      gtttacatg tggttggta attcccgac tatgtccca 
      ctggaaaaa ctaccatcg gccgctggg cttgacatg 
      ttggcacgt gggtgctgc attttgatt ggggggccg 
      attttaggg gcgttacgt gacgctact ggtagttac 
      gtaatatgc attattgta atgaattgt gttacgata 
      accacgata ttattgtgg ttaatagaa ggcatttta 
      acgaaatat acaaaaatt aaaatttta catagaaac 
      acttaa
    mRNA sequence
    • >TC032936 RA
      caattgtta taattggcg ttttttcca ttttcaaac 
      taaatcagt gcctggtag atgacgagt aggagccct 
      gtccgctct tagttttcc caaaacccg gattttaac 
      caaaaatga aagggccaa cgcatcctg atggaggct 
      acggctggg tggtggtct tcgccagcg cgctaacca 
      acttcatca ccctctctc taatccaat gtttcggcc 
      tcgtcctca aagacacgt tcgccgaat taaacctgt 
      cggcaactc aaggctcgc tcatcatta acttgaacg 
      gagctttcg gtcttctga ccggaccag tgactggag 
      ttttacaga aactgttcg gttgccgaa gaatcgcgt 
      tttttgggg ggcttttac tcacgttcg gaatggttc 
      tgacgtctt tctcccgaa gtttcgggt ctctggtcg 
      tcacttacg gactcgtaa cttcacttg ggatgcaaa 
      tgtgcgaat cgagccaaa ggctggcga ttaatcagt 
      atttcaaaa agaagagga gtttagtga cggggttta 
      taatgacag cggctggat ttggaccga ttttgatcc 
      cgcagctga tccgagttt tgctgaaaa attactcga 
      cgaatgagg tgacgctga tttatggcg ggttgtgtg 
      cgcatattc tcatagcgg gggcccttt tgcaaccaa 
      tcgagtggc actcgaagg aacaaggga cgaaaacgg 
      aaaaaatcg agctcaagg aacggaacg aaattatca 
      tttccgagg agaagttcc taagcaaaa ttgtggaca 
      atctggcga agattttcg atttggatt tgctcaaag 
      acccggtgt ttaataaca ttattatag gcctggctt 
      tggccacgt ttgccgaaa tcaatttca ctctccttg 
      tcccgttta ttctaaatg acttcaaat taaccacgg 
      atcaaatcg cgatttttc tctcgactt tagggattt 
      ccgacatga tctcgcgtt tcctgggcc cctatgttg 
      gtaattatt ttaccaaac cttcaaggc tcatgtttg 
      tctattctc ttgtaatcc tagttataa ttaaattta 
      caatactat gctccaaaa acttcattt tcctccttg 
      ttattgctt ttactttgg gtatagcca aaggaattc 
      gaaaggttt acatgtggt tggtaattc ccgactatg 
      tcccactgg aaaaactac catcggccg ctgggcttg 
      acatgttgg cacgtgggt gctgcattt tgattgggg 
      ggccgattt taggggcgt tacgtgacg ctactggta 
      gttacgtaa tatgcatta ttgtaatga attgtgtta 
      cgataacca cgatattat tgtggttaa tagaaggca 
      ttttaacga aatatacaa aaattaaaa ttttacata 
      gaaacactt aacgaaaca acaaaaata aaatgttta 
      tctgtgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY