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Gene information
General information
TC007068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 10124159..10126679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007068
    iBeetle screen iB_01119
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007068 RA
      MEVAKNGKT VKIDSPPKR RRLRVGDPD FAPPDGGWG 
      WLIVFACGF SNLSTFPMF QQFGLVFRQ KFADLGITN 
      PQTTTVINL NSAFNSCVG LLNGPIYRK FTYRQVAMF 
      GSLLVASSL FISTLCQSF WTYLIFYAM CYGTGIGVT 
      QAANALALN TYFKNRRRI ATGLSWSTT ALGPIVWPY 
      IITALLEIY GMEGTLLVF SSFALHAFL CSLLLQPVE 
      WHTKFREVE NGIPEAKPF LEKEPEGKL TKSSKSRSL 
      LSSQYLYNE DDPHATGYE IIDPGTPMM VKANDGWYS 
      QNRSLAGSR LSLASNKTN KTISRLPSG QNSVTMSKR 
      PSYNNLSKA ESKRNSSLN LNQEGKERK RKISQSKAA 
      IQEEEEHHE NNTLLAPEY HEKDVLKTA AKKLEEYKQ 
      EHDKDEEEE EVKPHEKIS FFRKVAIFF DFDLFKDPI 
      YVNLMLGIT IANFVEINF SIITPMVLE EFNFGKYQI 
      ATFMSLLGA TDILVRFFV PFIAEMIGW ENKTFFLVG 
      VLSMAFGRI ILVHTQTYI FGLVVAVII GAGKGLRTI 
      FMALVIPTH VPLERLPAA SGLQLATSG LLFLIMGPV 
      VGWIRDTVK NYVITLHIL NLLTYTTAI AWTIESYIS 
      RRKKGKSHA NGTKT
    CDS sequence
    • >TC007068 RA
      atggaagtt gcaaaaaac ggaaaaacg gtcaaaata 
      gactcgcca ccgaaacgg cgacgtctt cgagtcggg 
      gaccctgac ttcgcaccg ccagatggg ggttggggg 
      tggctcatt gtttttgct tgcggattc agtaatttg 
      agcacgttt ccaatgttt caacaattt ggcctcgtt 
      tttcggcaa aaattcgcc gatttgggg atcacaaac 
      ccgcaaact acgaccgtt attaattta aattcagcg 
      tttaattcg tgtgtgggt ttgttgaat ggtccaatt 
      tatcggaaa tttacgtac aggcaggtc gcaatgttc 
      ggctcactt ttagtagcc tcgtcgctt tttatctca 
      acgttgtgt cagtcgttt tggacctat ttaattttt 
      tatgctatg tgttacggg accgggatt ggagtcaca 
      caggccgct aatgcttta gctttgaat acttatttc 
      aaaaatcgg agaaggatt gcaacaggt ctatcgtgg 
      agtactaca gctctaggg cccattgtc tggccgtac 
      atcataaca gctcttttg gaaatttac ggaatggaa 
      gggacactg ctggttttt tcatctttt gccctacat 
      gcttttttg tgttcgttg cttctacaa ccagtcgaa 
      tggcatacg aaattccga gaggtcgaa aacggtatt 
      ccggaagct aaacctttc ctggagaaa gagccagaa 
      ggtaaattg accaagtct tcaaaatcg cgaagtctt 
      ctctccagt caatatctc tacaatgaa gatgatcca 
      catgccact ggttacgaa attatagac cctggtaca 
      cccatgatg gtcaaagct aacgatggg tggtattcc 
      caaaaccgg tcactggcc ggttctagg ctttctcta 
      gcctcgaat aaaacaaat aaaacaatt tcgaggtta 
      ccatcgggt caaaattca gtcacgatg tcaaaacga 
      ccatcttac aataaccta tcaaaagcc gagtctaaa 
      cgaaactct tcgttaaac ttaaaccaa gaaggtaaa 
      gaacgaaaa cgcaaaatt tcacagtcg aaagctgca 
      atacaagaa gaagaagaa catcacgaa aacaacaca 
      cttctcgca cctgaatat cacgagaaa gacgttctt 
      aaaaccgcg gccaagaag ttggaagag tacaagcag 
      gaacacgac aaagacgag gaagaagaa gaagtaaaa 
      ccgcacgaa aaaatctca ttcttccga aaagtcgcc 
      atttttttc gatttcgat ctcttcaaa gaccccatc 
      tatgttaat cttatgtta ggcatcacc attgccaac 
      tttgtcgaa ataaatttc tccataatc acaccaatg 
      gtcctcgaa gagttcaat tttggcaaa taccaaatt 
      gctactttt atgtcactg ttaggagcc accgacatt 
      cttgtccgg tttttcgta ccatttata gccgaaatg 
      atcggatgg gaaaataaa acttttttc cttgttgga 
      gtactaagt atggcgttt ggtcgaata attctggtc 
      cacactcaa acatacatt tttggtttg gtggtcgct 
      gttatcata ggcgccggc aaaggcctt agaacgatt 
      ttcatggcc ttggtaata ccgacacac gtaccttta 
      gaacgactt ccggctgct tctggtcta caattggcc 
      acaagtggt ctacttttt ctaatcatg ggacccgta 
      gtaggatgg attagagac acggtaaaa aattacgtc 
      attacgtta cacatactg aatttattg acgtacaca 
      acggctata gcatggacc attgagagt tacatcagt 
      agacggaaa aaaggcaaa agtcacgca aatgggacg 
      aagacgtga
    mRNA sequence
    • >TC007068 RA
      ttcgaacgg agagacgga aatatactc accggtagt 
      tttttttta tttcaagtg ccaaatagg aatcaaaaa 
      cgaacaaag acattccgc ccataaaca caaagccgg 
      tgaaataaa cgataacga aagtaaacc aagtcaatt 
      atctcaatt attctcctc acaaggcga cgattcctt 
      atgcaacta gagaaaacc acacatttt gcataattg 
      ccaaacacc tgtaaatag cgaaaatgg aagttgcaa 
      aaaacggaa aaacggtca aaatagact cgccaccga 
      aacggcgac gtcttcgag tcggggacc ctgacttcg 
      caccgccag atgggggtt gggggtggc tcattgttt 
      ttgcttgcg gattcagta atttgagca cgtttccaa 
      tgtttcaac aatttggcc tcgtttttc ggcaaaaat 
      tcgccgatt tggggatca caaacccgc aaactacga 
      ccgttatta atttaaatt cagcgttta attcgtgtg 
      tgggtttgt tgaatggtc caatttatc ggaaattta 
      cgtacaggc aggtcgcaa tgttcggct cacttttag 
      tagcctcgt cgcttttta tctcaacgt tgtgtcagt 
      cgttttgga cctatttaa ttttttatg ctatgtgtt 
      acgggaccg ggattggag tcacacagg ccgctaatg 
      ctttagctt tgaatactt atttcaaaa atcggagaa 
      ggattgcaa caggtctat cgtggagta ctacagctc 
      tagggccca ttgtctggc cgtacatca taacagctc 
      ttttggaaa tttacggaa tggaaggga cactgctgg 
      ttttttcat cttttgccc tacatgctt ttttgtgtt 
      cgttgcttc tacaaccag tcgaatggc atacgaaat 
      tccgagagg tcgaaaacg gtattccgg aagctaaac 
      ctttcctgg agaaagagc cagaaggta aattgacca 
      agtcttcaa aatcgcgaa gtcttctct ccagtcaat 
      atctctaca atgaagatg atccacatg ccactggtt 
      acgaaatta tagaccctg gtacaccca tgatggtca 
      aagctaacg atgggtggt attcccaaa accggtcac 
      tggccggtt ctaggcttt ctctagcct cgaataaaa 
      caaataaaa caatttcga ggttaccat cgggtcaaa 
      attcagtca cgatgtcaa aacgaccat cttacaata 
      acctatcaa aagccgagt ctaaacgaa actcttcgt 
      taaacttaa accaagaag gtaaagaac gaaaacgca 
      aaatttcac agtcgaaag ctgcaatac aagaagaag 
      aagaacatc acgaaaaca acacacttc tcgcacctg 
      aatatcacg agaaagacg ttcttaaaa ccgcggcca 
      agaagttgg aagagtaca agcaggaac acgacaaag 
      acgaggaag aagaagaag taaaaccgc acgaaaaaa 
      tctcattct tccgaaaag tcgccattt ttttcgatt 
      tcgatctct tcaaagacc ccatctatg ttaatctta 
      tgttaggca tcaccattg ccaactttg tcgaaataa 
      atttctcca taatcacac caatggtcc tcgaagagt 
      tcaattttg gcaaatacc aaattgcta cttttatgt 
      cactgttag gagccaccg acattcttg tccggtttt 
      tcgtaccat ttatagccg aaatgatcg gatgggaaa 
      ataaaactt ttttccttg ttggagtac taagtatgg 
      cgtttggtc gaataattc tggtccaca ctcaaacat 
      acatttttg gtttggtgg tcgctgtta tcataggcg 
      ccggcaaag gccttagaa cgattttca tggccttgg 
      taataccga cacacgtac ctttagaac gacttccgg 
      ctgcttctg gtctacaat tggccacaa gtggtctac 
      tttttctaa tcatgggac ccgtagtag gatggatta 
      gagacacgg taaaaaatt acgtcatta cgttacaca 
      tactgaatt tattgacgt acacaacgg ctatagcat 
      ggaccattg agagttaca tcagtagac ggaaaaaag 
      gcaaaagtc acgcaaatg ggacgaaga cgtgagaat 
      aattgtgat tgtatatat gaaggctgt ttttgtgta 
      tccaagtac ttaacattt gtgaaagaa atagtgaat 
      attttgacc gtttttaat aaaattttg ttactacc