Status
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Gene information
General information
TC007056 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10381205..10382963
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007056
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01116

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007056 RA
      MFALVLLCA LVSSSHGAR ILGVFMTPS YSHQVVFQP 
      IWKELSLRG HQVTVLTPN PINDPSLTN LTEIDLSFS 
      YKTWDNYSI ATLGTKEHS TSEEVKKLS EAYLAVSEV 
      QLSHPAVQE FLQNNTKYQ FDLILVEFL WPIMYGFKD 
      IYKCPMVGI SSLGLTATA MEAIGNPSN PALEPEFTL 
      PFSTNLSFK ERVISTVFK VLFKVGAQV SLRPKMEKL 
      KQKFFGNVR RLEVIAKDV SLVLVNSNL ALQNVKPLV 
      PAFVELSGI HLKKPKSLP PKLQKYLDE AKEGVIYFS 
      LGSNVKSKF LPKEQFGKF MSAFSELPY KVLWKFEKE 
      DMENKPDNV EIQKWLPQQ DLLRHPNIK LFITQAGLQ 
      SLDEAIRAQ VPMLTIPFF GDQRYNSDH LVQSGGALS 
      LDFHSFTSS EFKEKISEL ITNPSYKEK ITKLSKIAS 
      DQPMEALEK AVWWIEYVI RHDGAEHLR YAGVDMPFY 
      QYFLLDVIA FIIATLALI FYVLRRIFK HVVVCYKVV 
      KNVLKSLTT LKSHTE
    CDS sequence
    • >TC007056 RA
      atgtttgct ttggtgttg ttgtgtgct ctggtgtcc 
      tcgagccac ggtgcccga atcctcggg gtcttcatg 
      acccccagc tacagccac caggtggtc ttccaaccc 
      atctggaag gaattatcc ctgagggga caccaagtg 
      accgttctg acgcccaac ccaatcaac gaccccagt 
      ctaacgaat ctcaccgaa attgattta agcttctcg 
      tacaaaaca tgggataat tacagcatt gccacactg 
      gggaccaaa gaacattca acaagtgaa gaagtgaag 
      aagttgtca gaggcgtat ctggccgta agtgaagtc 
      cagttgagt catccagca gtgcaagaa ttcctccaa 
      aacaacacg aaataccag tttgattta atcctcgtt 
      gagtttctt tggcccata atgtacggc tttaaagac 
      atctacaag tgcccaatg gtcgggatt tcctcactc 
      ggactcacg gcaacggca atggaagcc atcggaaac 
      cccagcaat ccggccctg gaacccgaa ttcaccctc 
      cccttctcc acgaactta tcgttcaaa gaacgcgtg 
      attagtaca gtgtttaaa gttctgttc aaagtgggt 
      gctcaagtc tcacttcgg ccaaaaatg gagaaactc 
      aagcaaaaa tttttcggt aatgtgcgc cgattggaa 
      gtcatagca aaggacgta agccttgtc cttgtcaac 
      tccaacctg gccttgcaa aacgtcaag cctttggtg 
      cccgctttc gtcgaactg tccggaatt catttgaaa 
      aaacccaaa agtttgcca ccaaaactt cagaaatac 
      ctagacgaa gccaaggaa ggtgtgatc tacttcagt 
      cttggctcc aatgttaag agcaagttt ttgccgaaa 
      gaacagttt gggaaattc atgagtgcc ttctcggaa 
      ctgccttac aaagtcttg tggaagttt gagaaagaa 
      gatatggag aataagccg gacaatgtt gagattcag 
      aaatggttg ccacagcaa gacctgctc cggcaccca 
      aacatcaaa ctgtttatt acccaagcg gggcttcag 
      tcgctggac gaagccatc cgggcccaa gtccccatg 
      ctcacaatt cccttcttc ggggaccag aggtacaac 
      agcgaccat ttggtccag agtggagga gccctctcg 
      cttgatttc cattcgttt acttcgagc gaatttaaa 
      gagaaaatc agcgaactc atcacaaat ccatcgtac 
      aaggagaaa attacaaaa ctgtcaaag atagcaagc 
      gatcagccg atggaagct ttggaaaaa gccgtgtgg 
      tggattgaa tacgttata aggcacgat ggggctgaa 
      catttgaga tacgccggt gtcgatatg ccattttac 
      cagtatttc ctcctagac gtaattgct tttatcata 
      gctacactc gccttaata ttctacgtc ctgcgaaga 
      atattcaag cacgttgtc gtgtgctat aaagtcgtc 
      aaaaacgta ctcaaatca ctcacgacg cttaagagt 
      cacactgaa taa
    mRNA sequence
    • >TC007056 RA
      ctttccaaa atgtttgct ttggtgttg ttgtgtgct 
      ctggtgtcc tcgagccac ggtgcccga atcctcggg 
      gtcttcatg acccccagc tacagccac caggtggtc 
      ttccaaccc atctggaag gaattatcc ctgagggga 
      caccaagtg accgttctg acgcccaac ccaatcaac 
      gaccccagt ctaacgaat ctcaccgaa attgattta 
      agcttctcg tacaaaaca tgggataat tacagcatt 
      gccacactg gggaccaaa gaacattca acaagtgaa 
      gaagtgaag aagttgtca gaggcgtat ctggccgta 
      agtgaagtc cagttgagt catccagca gtgcaagaa 
      ttcctccaa aacaacacg aaataccag tttgattta 
      atcctcgtt gagtttctt tggcccata atgtacggc 
      tttaaagac atctacaag tgcccaatg gtcgggatt 
      tcctcactc ggactcacg gcaacggca atggaagcc 
      atcggaaac cccagcaat ccggccctg gaacccgaa 
      ttcaccctc cccttctcc acgaactta tcgttcaaa 
      gaacgcgtg attagtaca gtgtttaaa gttctgttc 
      aaagtgggt gctcaagtc tcacttcgg ccaaaaatg 
      gagaaactc aagcaaaaa tttttcggt aatgtgcgc 
      cgattggaa gtcatagca aaggacgta agccttgtc 
      cttgtcaac tccaacctg gccttgcaa aacgtcaag 
      cctttggtg cccgctttc gtcgaactg tccggaatt 
      catttgaaa aaacccaaa agtttgcca ccaaaactt 
      cagaaatac ctagacgaa gccaaggaa ggtgtgatc 
      tacttcagt cttggctcc aatgttaag agcaagttt 
      ttgccgaaa gaacagttt gggaaattc atgagtgcc 
      ttctcggaa ctgccttac aaagtcttg tggaagttt 
      gagaaagaa gatatggag aataagccg gacaatgtt 
      gagattcag aaatggttg ccacagcaa gacctgctc 
      cggcaccca aacatcaaa ctgtttatt acccaagcg 
      gggcttcag tcgctggac gaagccatc cgggcccaa 
      gtccccatg ctcacaatt cccttcttc ggggaccag 
      aggtacaac agcgaccat ttggtccag agtggagga 
      gccctctcg cttgatttc cattcgttt acttcgagc 
      gaatttaaa gagaaaatc agcgaactc atcacaaat 
      ccatcgtac aaggagaaa attacaaaa ctgtcaaag 
      atagcaagc gatcagccg atggaagct ttggaaaaa 
      gccgtgtgg tggattgaa tacgttata aggcacgat 
      ggggctgaa catttgaga tacgccggt gtcgatatg 
      ccattttac cagtatttc ctcctagac gtaattgct 
      tttatcata gctacactc gccttaata ttctacgtc 
      ctgcgaaga atattcaag cacgttgtc gtgtgctat 
      aaagtcgtc aaaaacgta ctcaaatca ctcacgacg 
      cttaagagt cacactgaa taatcgacc aatctcaac 
      taattctat ttattaagc cttataggc tccattttt 
      ctacttctc ccctagtaa ataaagttt taacacagg 
      a
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