Status
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Gene information
General information
TC034674 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10609048..10640799
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034674
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01115

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034674 RA
      MNTISGLSL HLLELRVPS PVVRNQDVR LECHFDLDD 
      EVLYSVKWY KDGNEFYRY VPRDMPPAQ VFHLPGVKV 
      DIHNSSESA VVLSSVELS SSGVYRCEV SGEAPFFQT 
      VTEHREMLV VALPEEGPR ISGGRPRYQ VGDTVRVNC 
      TSGRSKPAA NLKWMINGE PADPKFLVP HKKIITGRE 
      GLETTVLGL QFVVRHKHF KRGDMKLKC VASIESVYS 
      RSNEESVEG ERPQRASVL ESRGTVAPA GSRADRVQA 
      AGSRKAFLP HVSLVAMCC FLLALCPR
    CDS sequence
    • >TC034674 RA
      atgaataca ataagtggg ctctcgttg cacttactg 
      gaacttcga gttcctagt ccggtggtg aggaaccag 
      gacgttcgt cttgagtgc cacttcgac ttggacgac 
      gaggtctta tattccgta aagtggtat aaggacggc 
      aatgagttc tatcgctac gtaccccgt gacatgcca 
      cctgctcaa gtcttccat cttccgggc gtcaaggtc 
      gatatccac aactccagc gagtctgct gtcgttttg 
      tcttccgtt gagctctcc agcagcggt gtttacaga 
      tgcgaggtc tctggagag gctcccttc tttcagacc 
      gtcaccgaa caccgcgag atgttggtc gtagcattg 
      ccggaagag ggcccccga atatcaggt ggccgtccc 
      cgctatcaa gttggtgat acggttcga gtgaattgc 
      acgtcgggt cggtctaaa cccgccgcc aatttgaaa 
      tggatgatc aacggagag ccggcggat ccgaaattc 
      ctcgttccc cacaaaaag atcatcact ggacgagaa 
      ggactcgag acgacggtt ctgggactg cagtttgtc 
      gtgcgtcat aagcacttc aaacgagga gatatgaag 
      ctcaaatgc gtcgcttcg atcgaaagt gtttattcg 
      agaagtaat gaagagagt gttgagggc gaacggccg 
      cagagagcc tcggttctg gaaagtcga gggacggtg 
      gcccccgcc ggctctagg gccgacagg gtgcaagcc 
      gcaggaagc cggaaagcg tttctccct catgtttcc 
      ctcgtcgcg atgtgttgt tttttactc gcattatgt 
      ccaaggtaa
    mRNA sequence
    • >TC034674 RA
      attatcact gggaagaga aacaatgaa tacaataag 
      tgggctctc gttgcactt actggaact tcgagttcc 
      tagtccggt ggtgaggaa ccaggacgt tcgtcttga 
      gtgccactt cgacttgga cgacgaggt cttatattc 
      cgtaaagtg gtataagga cggcaatga gttctatcg 
      ctacgtacc ccgtgacat gccacctgc tcaagtctt 
      ccatcttcc gggcgtcaa ggtcgatat ccacaactc 
      cagcgagtc tgctgtcgt tttgtcttc cgttgagct 
      ctccagcag cggtgttta cagatgcga ggtctctgg 
      agaggctcc cttctttca gaccgtcac cgaacaccg 
      cgagatgtt ggtcgtagc attgccgga agagggccc 
      ccgaatatc aggtggccg tccccgcta tcaagttgg 
      tgatacggt tcgagtgaa ttgcacgtc gggtcggtc 
      taaacccgc cgccaattt gaaatggat gatcaacgg 
      agagccggc ggatccgaa attcctcgt tccccacaa 
      aaagatcat cactggacg agaaggact cgagacgac 
      ggttctggg actgcagtt tgtcgtgcg tcataagca 
      cttcaaacg aggagatat gaagctcaa atgcgtcgc 
      ttcgatcga aagtgttta ttcgagaag taatgaaga 
      gagtgttga gggcgaacg gccgcagag agcctcggt 
      tctggaaag tcgagggac ggtggcccc cgccggctc 
      tagggccga cagggtgca agccgcagg aagccggaa 
      agcgtttct ccctcatgt ttccctcgt cgcgatgtg 
      ttgtttttt actcgcatt atgtccaag gtaattgtg 
      ttctcttga tcagcttct tgctaataa gccaaagac 
      acttgtgtg agtgatatg aatttaact gttgctttg 
      tacattcct ttattattg ctttgtttt tcgtcctgt 
      taaagtttc tgtagtttc atatggcac taatttaag 
      aattaagct ttaagttcg gtatatgtt tataaaatg 
      tagccattt actattgta catatctat aattaataa 
      atgtttcca acaatcaaa tcactcttc tagtcgtat 
      catttcggg catcgagct tgccatttt tatccagaa 
      atgtaagta tcataccta gattgtaaa tttaaacgc 
      tttgttcca tcaaagaga ggaattgag cctttaaat 
      gatatgtga gcacactgt tcatgtcat tgtaattaa 
      agaattttc catataatt accaaaatt aaggctagt 
      ctgttaatg aaactgaat acgtttatg atttaaatt 
      ttaggggaa aaattgtat aaatatctc aagtgttat 
      gtgcatata cgagaaaac acgttagcg cttaaggtt 
      tatctttga taatgcact tatttcaat ctaagtccc 
      gcatgaaat tttttggac aagataagt tttgattga 
      tttccgtta gctgtctta gttcctgaa atttatctt 
      gccaaaatt tcatgttac tttacatta aaataaatc 
      cctgtgatg aaaaaaaat tgtaaggtt gacgacgag 
      gtcaattgg tggttaatt aggacgagt cgaacgcct 
      cgttcgact ataactaaa tttatatgt agcgaagat 
      taattttaa atattgtgt aattactct aaaattaac 
      taaatgtat atataacgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY