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Gene information
General information
TC007034 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 11251452..11262861
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007034
    iBeetle screen iB_01113
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 14.29% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    head ventral surface irregular - Number of animals showing the phenotype: 8


    elytra shape irregular - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007034 RA
      MASPFKKEE VYGFKTLYR ELGYYISRL EQYERALKF 
      FDEAIAKTP NDRRALMGR AWARSKACK YEGALDDIR 
      TALNLDPDD LVMLAHKAL NTYLNCEFE DGLVQNTRL 
      LPIRKKPDY FQMGAMHCA DAIENCLGE RAGRPLRDH 
      YLIIRRLAW KKNFEEQKR FQPKSRYKK KKKKKIVLP 
      DKVTSKGDP IIPLKSSRN LNQQSLTSE NTLTQVDEP 
      PPDRESIVD SIYSLKSER NILPPPIKF PYRPLQRYT 
      SNIENYMAE KYLDTLYKD KLFLKNLPT EPGISCPNE 
      AGTKKILLL AKTGYKTVK YKQELLRAR RPFYFIKYQ 
      EATSSGALK VRQEQQLNK LRDAAKKEA DSIVSKMKD 
      ALEEKGWKS VLETAEKLK TFCDLKSRK ILPDRDTYL 
      EVLYDTVCQ AHYDLKRIN KNQLEWDQE KRIYKMLGL 
      HLSREPSTD SVITQFKGV FIDWKKQIT VYGDRLRKA 
      TTPKEMCWL YHELSRFHT ELKQYELAR VYARKCMNE 
      GKRTENNKW VINALLLIM RINIAQHSK NDAKSDAQA 
      AIELATQMG DKNLIAFLN KCKQVIEKV AFDEKLGPK 
      VLMQREQKI VEMMAGDKM KDEAAHLFR MMSAMPAAR 
      RMSVMPGIR ITDDDDDKK AMASRKQSI MPGVPKPAE 
      FAMTTPTQK QKKGGKQVS IDESSKGVG FVELIKYHI 
      DD
    CDS sequence
    • >TC007034 RA
      atggcctca ccctttaaa aaagaagaa gtttatggg 
      tttaaaacc ctctaccgc gagcttggc tattacata 
      tcccgattg gagcaatat gagagagct ttgaaattt 
      ttcgatgaa gctattgcc aaaacacca aatgaccga 
      agagctcta atggggcgc gcttgggcc aggtctaag 
      gcttgtaaa tacgagggg gctcttgat gacatcaga 
      acggccctt aatttggac cccgatgac cttgtcatg 
      ttggcacat aaggccctc aacacttat ctaaattgt 
      gaatttgag gatgggtta gtgcaaaac acgcggctt 
      ctgccgata cgcaaaaaa cctgattac ttccaaatg 
      ggcgcaatg cattgtgct gatgctatt gaaaactgt 
      ttgggtgaa cgagccgga aggccgtta cgtgaccac 
      tacttaata attcggcga ttagcttgg aaaaaaaat 
      ttcgaagaa cagaaacgc ttccagccc aaatcacgt 
      tacaagaag aaaaagaag aagaaaatc gttcttccc 
      gataaagtg accagtaaa ggcgatcct attattccc 
      ctaaaatca tcacgaaac ttgaatcaa caatcttta 
      acctctgag aatactcta acccaagtt gatgaacct 
      ccacccgat cgcgaaagc attgttgac agcatctac 
      tcgttaaaa tcagaacgc aacattctt ccacccccc 
      attaaattc ccttacaga ccccttcag cgatacacc 
      agcaacatt gagaactat atggccgaa aaatacctc 
      gacacactt tacaaagac aaacttttt ctcaaaaat 
      ctcccaaca gagcctgga atttcttgc cccaatgaa 
      gccgggaca aaaaaaatt ttactactg gctaagacc 
      ggctacaaa acggttaaa tacaaacaa gagttgtta 
      cgagccaga cgacctttt tatttcatc aagtaccaa 
      gaagctact tcttctggt gccctcaag gtgcgacaa 
      gagcaacaa ctgaacaaa ttaagggac gcagcaaag 
      aaagaagca gattcgata gtctccaaa atgaaggat 
      gctcttgaa gaaaagggt tggaagtca gtgcttgaa 
      acggccgag aaattgaaa actttctgc gacctcaaa 
      tctcgcaaa atccttcct gatcgtgac acttatttg 
      gaagtgttg tacgatacg gtgtgtcaa gcacattac 
      gatttgaag cgtatcaac aagaaccag ctcgagtgg 
      gatcaagag aagaggatt tataaaatg ttggggctc 
      catttaagt cgggaacca tcaaccgat tcggttatc 
      actcagttt aagggagtt tttatcgat tggaagaag 
      cagattact gtatatggt gatcggtta cgtaaagca 
      acaacaccc aaagaaatg tgttggctg taccatgaa 
      ctgagccga ttccatact gagttgaag caatatgaa 
      ttagcccgt gtctatgcc aggaaatgt atgaacgag 
      ggcaaacgt accgaaaat aataaatgg gtcatcaat 
      gctttgcta cttatcatg aggattaac atagctcaa 
      catagcaaa aatgatgcc aaatcggat gcgcaagct 
      gctattgaa ctggcgacg caaatgggt gataagaac 
      ctaatagct ttcttaaac aaatgcaag caagtgatc 
      gaaaaagtt gcctttgac gagaaacta ggcccgaaa 
      gtattgatg cagcgagag caaaagatt gtcgagatg 
      atggctggt gataagatg aaagacgaa gctgctcat 
      cttttcagg atgatgtct gcaatgcca gctgccaga 
      cgcatgtct gttatgcct ggaatcagg atcactgat 
      gacgacgat gataagaaa gcaatggct tcaaggaaa 
      caatccata atgccagga gtgccaaaa ccggctgag 
      ttcgcaatg acgacgccc actcagaag cagaagaaa 
      ggaggcaag caagtttca atagatgag tcgtcgaaa 
      ggggtcgga tttgttgaa cttatcaaa tatcatatc 
      gatgactga
    mRNA sequence
    • >TC007034 RA
      tttcaattg cttaatgaa ttattattt actaaaagt 
      ttgccggtt actttaata tgaaagata cttttagca 
      tacgcaccc tgtatagtt ttttgaaaa agtgctgag 
      tttgtacct taaaatcgt tctttattt ttttaatat 
      ttttgacaa aatttcact gaatttatg taatattct 
      ttttaagtc agaacccgt tcctgctta tgacagcct 
      gtttaaaaa taaatcgta gccataacg gaccattta 
      tggttatta aggtaattt cacagggtg ttgaggaaa 
      tgaatatca gatttagtt aacagtgat tttttttta 
      attctgatg ttttactgc cattcacat cgtttcgaa 
      ttttcctaa atctagtct tactgtaag atagaatca 
      ttagaactc ttttaggat aaattttat tgaagaata 
      tttacaaga ggccttcaa acatgttag tttcattgt 
      cattgcctt agattagat tagtgtgtg tttaatgtg 
      taaaagtgg gtattttta taatactta tgctacatt 
      cactagaaa tgtaaatat aacgacttc actaaacta 
      ttttttaac tcattaatc tgttaagtc taacattaa 
      agaacaaat atatccgcc acatctacc cgaaaaatt 
      agattgtaa gattagttt gtaagaatt ttttttcaa 
      tttcgaatt ttttacaca caaggtcat tacaccatg 
      atgtagaaa atcacaaga taattgtaa atttgttgt 
      tgaatcaaa taaccataa attttatac aacttctct 
      cccaacttt ttgtgcagt tccagtagc tgtaacaag 
      acgtaatta tttactgtt gaactgttt ttggagctt 
      agtctttag attagtgct taatactaa ctaacattt 
      ttttattat gcgtgtgct gaaattgtt taataaaag 
      cttgaaact ttcattttt gacaatttg tcaaagttt 
      ttgtccact aaattatgg cctcaccct ttaaaaaag 
      aagaagttt atgggttta aaaccctct accgcgagc 
      ttggctatt acatatccc gattggagc aatatgaga 
      gagctttga aatttttcg atgaagcta ttgccaaaa 
      caccaaatg accgaagag ctctaatgg ggcgcgctt 
      gggccaggt ctaaggctt gtaaatacg agggggctc 
      ttgatgaca tcagaacgg cccttaatt tggaccccg 
      atgaccttg tcatgttgg cacataagg ccctcaaca 
      cttatctaa attgtgaat ttgaggatg ggttagtgc 
      aaaacacgc ggcttctgc cgatacgca aaaaacctg 
      attacttcc aaatgggcg caatgcatt gtgctgatg 
      ctattgaaa actgtttgg gtgaacgag ccggaaggc 
      cgttacgtg accactact taataattc ggcgattag 
      cttggaaaa aaaatttcg aagaacaga aacgcttcc 
      agcccaaat cacgttaca agaagaaaa agaagaaga 
      aaatcgttc ttcccgata aagtgacca gtaaaggcg 
      atcctatta ttcccctaa aatcatcac gaaacttga 
      atcaacaat ctttaacct ctgagaata ctctaaccc 
      aagttgatg aacctccac ccgatcgcg aaagcattg 
      ttgacagca tctactcgt taaaatcag aacgcaaca 
      ttcttccac cccccatta aattccctt acagacccc 
      ttcagcgat acaccagca acattgaga actatatgg 
      ccgaaaaat acctcgaca cactttaca aagacaaac 
      tttttctca aaaatctcc caacagagc ctggaattt 
      cttgcccca atgaagccg ggacaaaaa aaattttac 
      tactggcta agaccggct acaaaacgg ttaaataca 
      aacaagagt tgttacgag ccagacgac ctttttatt 
      tcatcaagt accaagaag ctacttctt ctggtgccc 
      tcaaggtgc gacaagagc aacaactga acaaattaa 
      gggacgcag caaagaaag aagcagatt cgatagtct 
      ccaaaatga aggatgctc ttgaagaaa agggttgga 
      agtcagtgc ttgaaacgg ccgagaaat tgaaaactt 
      tctgcgacc tcaaatctc gcaaaatcc ttcctgatc 
      gtgacactt atttggaag tgttgtacg atacggtgt 
      gtcaagcac attacgatt tgaagcgta tcaacaaga 
      accagctcg agtgggatc aagagaaga ggatttata 
      aaatgttgg ggctccatt taagtcggg aaccatcaa 
      ccgattcgg ttatcactc agtttaagg gagttttta 
      tcgattgga agaagcaga ttactgtat atggtgatc 
      ggttacgta aagcaacaa cacccaaag aaatgtgtt 
      ggctgtacc atgaactga gccgattcc atactgagt 
      tgaagcaat atgaattag cccgtgtct atgccagga 
      aatgtatga acgagggca aacgtaccg aaaataata 
      aatgggtca tcaatgctt tgctactta tcatgagga 
      ttaacatag ctcaacata gcaaaaatg atgccaaat 
      cggatgcgc aagctgcta ttgaactgg cgacgcaaa 
      tgggtgata agaacctaa tagctttct taaacaaat 
      gcaagcaag tgatcgaaa aagttgcct ttgacgaga 
      aactaggcc cgaaagtat tgatgcagc gagagcaaa 
      agattgtcg agatgatgg ctggtgata agatgaaag 
      acgaagctg ctcatcttt tcaggatga tgtctgcaa 
      tgccagctg ccagacgca tgtctgtta tgcctggaa 
      tcaggatca ctgatgacg acgatgata agaaagcaa 
      tggcttcaa ggaaacaat ccataatgc caggagtgc 
      caaaaccgg ctgagttcg caatgacga cgcccactc 
      agaagcaga agaaaggag gcaagcaag tttcaatag 
      atgagtcgt cgaaagggg tcggatttg ttgaactta 
      tcaaatatc atatcgatg actgacttg agataaata 
      aagatgaac tttgtaata agagtgaca tttcagttg 
      tgacaaact gtctaaaaa attttaaat atttcacta 
      aagagaaga ttttgctct tggaattgg gcgcagaaa 
      ctacgattt cgtaaagta aatatgaat aggtaacca 
      aacctttta aacactttt agtgttatt cgaggaaca 
      acaaaacaa acatttact ttgaaggtg tatcttatc 
      gacctgtgc tctacatta ccaacacat ttcctcgaa 
      aaagtctac ccacattta tttgtacaa aagtaattt 
      tttaatttt ccacaaaaa tctaaaccc aatcgatgc 
      ctaattaaa gtttatggc agtataaaa ggtaaacaa 
      tttgcgtgt aagacaaca ccgtttatt aaacactta 
      gtcataagt gagcagatt ttgcaaaca aaccaagtt 
      ttaccaaat cttcataaa aatcaatat taagatgta 
      ggtgaacat ttaattatt atttacgtc ttggtttca 
      agtaacgac acattagtg tgacatcga ctgattctg 
      agtacagtt ttcatactt ttgtctgaa ttccaataa 
      cctgtttag tggtaaata aattagtac tatcggc