Status
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Gene information
General information
TC032906
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007031
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11297832..11303801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032906
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01112

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032906 RA
      MAFAQLVSA LILSSFFLT VTPTEVPES VTNEIKELF 
      KTGQAPKNL AKDLENFRM PRLFREGME NTYEHPVIC 
      FFCNLLVDL AIGERRLGA SREVLAKEI EFICVHLGI 
      ESERVCTGV IDLNIDVFL YIVDNYPNL DSHRVCGSV 
      LQSLGCSPG DNFEWSIEL PSGSSPVRP KPNDTLDSF 
      KILQLSDIH YDPNYKPYG NADCGEPIC CQTDQGEPS 
      SPENTCGYW TDYREADVP WHLIEETVR QAKTQEFEY 
      VYYTGDIIS HRVWETSIR NNSQSISDL FTYFKQSFD 
      VPVYPVFGN HEPHPLDLW PTESVKDEK FSVQWLFEL 
      VSGPWSELV GQDISETVL KGGYYTVSP KPGFRIIAI 
      NGNLCYTYN WWLIFNDVD PYGQLQWLT ETLLEAEKN 
      NESVHILSH VPSGTSESL SVWGREYSR IIERFANTI 
      TGQFNGHTH RDQFHVYYN SSNPTQPIG TAFNGASVT 
      PYTASNPSY KIYDVDSTT FDLLDYEEW TFNLTLANS 
      KSSNESPDW YKLYSFTEA FGVDNLSPV EVDKVLYKM 
      AENHSLLDD YFLFKFRNG DAGIKDGCD DKCKKDNLC 
      EIATTVYGV DVHCNRFTD LYDKGQ
    CDS sequence
    • >TC032906 RA
      atggcgttc gcccagtta gtttctgct ttaattttg 
      agttcgttt ttcttaaca gtaacacca accgaagtt 
      ccagaatcg gtcacaaat gaaatcaag gaattattc 
      aaaacggga caggctccc aaaaatctt gccaaagat 
      ttggaaaat ttccgaatg cctcgcttg ttccgcgaa 
      ggcatggaa aatacctac gaacaccct gtgatttgc 
      ttcttttgc aatttgttg gtcgatttg gctatcggt 
      gagcgccgg ttgggggca agtcgtgaa gttctagcc 
      aaagaaatt gagtttatt tgtgttcat ctgggtatt 
      gagagcgag cgcgtttgc acgggagta attgatctg 
      aacatagac gtttttctt tatatcgtt gacaattac 
      ccaaatctt gactcgcat cgagtttgt gggtccgtt 
      cttcagtct ttgggttgc tcacctggg gataatttt 
      gaatggtca attgaactc cctagtggt agttcgcca 
      gttagacct aaacccaac gatacgctg gatagtttc 
      aaaattttg caattgtct gatattcac tatgacccg 
      aattataaa ccgtatgga aatgctgat tgtggtgaa 
      cccatctgt tgtcaaact gaccaaggg gagcctagt 
      tcacccgaa aatacatgc gggtattgg acggattac 
      cgcgaggct gatgtgccg tggcatttg atcgaagaa 
      acagtcaga caggccaaa actcaggag ttcgagtac 
      gtttactac accggtgat atcatcagt caccgggtt 
      tgggaaacc agcattcgg aataactcg caatcaatc 
      tccgatctt ttcacctac tttaaacaa agtttcgac 
      gtcccagtt tacccagtt tttggaaac cacgaacct 
      catccgctt gacctctgg cccactgaa agcgtcaaa 
      gacgagaaa ttctcagtc cagtggctc tttgagcta 
      gtttcaggc ccttggtct gaactcgta ggccaagat 
      ataagcgaa actgttcta aaagggggt tactataca 
      gttagtcca aaacccgga tttcgaatt atcgcaatt 
      aatggcaat ctttgttac acctacaac tggtggctc 
      attttcaac gatgtcgac ccttacgga cagttgcaa 
      tggctgact gaaacgctt cttgaagcc gagaaaaac 
      aacgaaagc gtccacatt ttatcgcac gttccgtca 
      ggaacgtct gaaagttta tcagtttgg gggcgcgaa 
      tattccaga attattgaa agattcgca aataccatc 
      actggccag ttcaatggg cacacacac agggaccag 
      tttcacgtt tattataac agttcaaac ccaacacaa 
      ccaataggc actgctttt aacggggct tcggtaaca 
      ccttacacc gcctctaac cccagttat aaaatttac 
      gacgtggat tcgaccact tttgatttg ctcgactac 
      gaagaatgg acgtttaat ctcacgtta gccaactct 
      aaaagttcg aatgagtct cccgattgg tacaaattg 
      tattctttt actgaagct ttcggagtt gataattta 
      tcgccagtt gaggttgat aaagttttg tataaaatg 
      gcagagaat cactcatta cttgacgat tactttttg 
      ttcaaattt cgaaatggt gatgctgga attaaggat 
      ggctgtgat gacaagtgc aagaaagat aatctgtgt 
      gagattgca acaactgtg tatggagtg gacgtccac 
      tgtaatcgt ttcactgat ctgtatgac aaaggacaa 
      taa
    mRNA sequence
    • >TC032906 RA
      cgacttacc tacatccat tcattacga tggcgttcg 
      cccagttag tttctgctt taattttga gttcgtttt 
      tcttaacag taacaccaa ccgaagttc cagaatcgg 
      tcacaaatg aaatcaagg aattattca aaacgggac 
      aggctccca aaaatcttg ccaaagatt tggaaaatt 
      tccgaatgc ctcgcttgt tccgcgaag gcatggaaa 
      atacctacg aacaccctg tgatttgct tcttttgca 
      atttgttgg tcgatttgg ctatcggtg agcgccggt 
      tgggggcaa gtcgtgaag ttctagcca aagaaattg 
      agtttattt gtgttcatc tgggtattg agagcgagc 
      gcgtttgca cgggagtaa ttgatctga acatagacg 
      tttttcttt atatcgttg acaattacc caaatcttg 
      actcgcatc gagtttgtg ggtccgttc ttcagtctt 
      tgggttgct cacctgggg ataattttg aatggtcaa 
      ttgaactcc ctagtggta gttcgccag ttagaccta 
      aacccaacg atacgctgg atagtttca aaattttgc 
      aattgtctg atattcact atgacccga attataaac 
      cgtatggaa atgctgatt gtggtgaac ccatctgtt 
      gtcaaactg accaagggg agcctagtt cacccgaaa 
      atacatgcg ggtattgga cggattacc gcgaggctg 
      atgtgccgt ggcatttga tcgaagaaa cagtcagac 
      aggccaaaa ctcaggagt tcgagtacg tttactaca 
      ccggtgata tcatcagtc accgggttt gggaaacca 
      gcattcgga ataactcgc aatcaatct ccgatcttt 
      tcacctact ttaaacaaa gtttcgacg tcccagttt 
      acccagttt ttggaaacc acgaacctc atccgcttg 
      acctctggc ccactgaaa gcgtcaaag acgagaaat 
      tctcagtcc agtggctct ttgagctag tttcaggcc 
      cttggtctg aactcgtag gccaagata taagcgaaa 
      ctgttctaa aagggggtt actatacag ttagtccaa 
      aacccggat ttcgaatta tcgcaatta atggcaatc 
      tttgttaca cctacaact ggtggctca ttttcaacg 
      atgtcgacc cttacggac agttgcaat ggctgactg 
      aaacgcttc ttgaagccg agaaaaaca acgaaagcg 
      tccacattt tatcgcacg ttccgtcag gaacgtctg 
      aaagtttat cagtttggg ggcgcgaat attccagaa 
      ttattgaaa gattcgcaa ataccatca ctggccagt 
      tcaatgggc acacacaca gggaccagt ttcacgttt 
      attataaca gttcaaacc caacacaac caataggca 
      ctgctttta acggggctt cggtaacac cttacaccg 
      cctctaacc ccagttata aaatttacg acgtggatt 
      cgaccactt ttgatttgc tcgactacg aagaatgga 
      cgtttaatc tcacgttag ccaactcta aaagttcga 
      atgagtctc ccgattggt acaaattgt attctttta 
      ctgaagctt tcggagttg ataatttat cgccagttg 
      aggttgata aagttttgt ataaaatgg cagagaatc 
      actcattac ttgacgatt actttttgt tcaaatttc 
      gaaatggtg atgctggaa ttaaggatg gctgtgatg 
      acaagtgca agaaagata atctgtgtg agattgcaa 
      caactgtgt atggagtgg acgtccact gtaatcgtt 
      tcactgatc tgtatgaca aaggacaat aatgtttaa 
      tttatttga tgaacttgg tacctgaat aaaatgctt 
      gaaccatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY