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Gene information
General information
TC032906
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007031 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 11297832..11303801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032906
    iBeetle screen iB_01112
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032906 RA
      MAFAQLVSA LILSSFFLT VTPTEVPES VTNEIKELF 
KTGQAPKNL AKDLENFRM PRLFREGME NTYEHPVIC 
FFCNLLVDL AIGERRLGA SREVLAKEI EFICVHLGI 
ESERVCTGV IDLNIDVFL YIVDNYPNL DSHRVCGSV 
LQSLGCSPG DNFEWSIEL PSGSSPVRP KPNDTLDSF 
KILQLSDIH YDPNYKPYG NADCGEPIC CQTDQGEPS 
SPENTCGYW TDYREADVP WHLIEETVR QAKTQEFEY 
VYYTGDIIS HRVWETSIR NNSQSISDL FTYFKQSFD 
VPVYPVFGN HEPHPLDLW PTESVKDEK FSVQWLFEL 
VSGPWSELV GQDISETVL KGGYYTVSP KPGFRIIAI 
NGNLCYTYN WWLIFNDVD PYGQLQWLT ETLLEAEKN 
NESVHILSH VPSGTSESL SVWGREYSR IIERFANTI 
TGQFNGHTH RDQFHVYYN SSNPTQPIG TAFNGASVT 
PYTASNPSY KIYDVDSTT FDLLDYEEW TFNLTLANS 
KSSNESPDW YKLYSFTEA FGVDNLSPV EVDKVLYKM 
AENHSLLDD YFLFKFRNG DAGIKDGCD DKCKKDNLC 
EIATTVYGV DVHCNRFTD LYDKGQ
    CDS sequence
    • >TC032906 RA
      atggcgttc gcccagtta gtttctgct ttaattttg 
agttcgttt ttcttaaca gtaacacca accgaagtt 
ccagaatcg gtcacaaat gaaatcaag gaattattc 
aaaacggga caggctccc aaaaatctt gccaaagat 
ttggaaaat ttccgaatg cctcgcttg ttccgcgaa 
ggcatggaa aatacctac gaacaccct gtgatttgc 
ttcttttgc aatttgttg gtcgatttg gctatcggt 
gagcgccgg ttgggggca agtcgtgaa gttctagcc 
aaagaaatt gagtttatt tgtgttcat ctgggtatt 
gagagcgag cgcgtttgc acgggagta attgatctg 
aacatagac gtttttctt tatatcgtt gacaattac 
ccaaatctt gactcgcat cgagtttgt gggtccgtt 
cttcagtct ttgggttgc tcacctggg gataatttt 
gaatggtca attgaactc cctagtggt agttcgcca 
gttagacct aaacccaac gatacgctg gatagtttc 
aaaattttg caattgtct gatattcac tatgacccg 
aattataaa ccgtatgga aatgctgat tgtggtgaa 
cccatctgt tgtcaaact gaccaaggg gagcctagt 
tcacccgaa aatacatgc gggtattgg acggattac 
cgcgaggct gatgtgccg tggcatttg atcgaagaa 
acagtcaga caggccaaa actcaggag ttcgagtac 
gtttactac accggtgat atcatcagt caccgggtt 
tgggaaacc agcattcgg aataactcg caatcaatc 
tccgatctt ttcacctac tttaaacaa agtttcgac 
gtcccagtt tacccagtt tttggaaac cacgaacct 
catccgctt gacctctgg cccactgaa agcgtcaaa 
gacgagaaa ttctcagtc cagtggctc tttgagcta 
gtttcaggc ccttggtct gaactcgta ggccaagat 
ataagcgaa actgttcta aaagggggt tactataca 
gttagtcca aaacccgga tttcgaatt atcgcaatt 
aatggcaat ctttgttac acctacaac tggtggctc 
attttcaac gatgtcgac ccttacgga cagttgcaa 
tggctgact gaaacgctt cttgaagcc gagaaaaac 
aacgaaagc gtccacatt ttatcgcac gttccgtca 
ggaacgtct gaaagttta tcagtttgg gggcgcgaa 
tattccaga attattgaa agattcgca aataccatc 
actggccag ttcaatggg cacacacac agggaccag 
tttcacgtt tattataac agttcaaac ccaacacaa 
ccaataggc actgctttt aacggggct tcggtaaca 
ccttacacc gcctctaac cccagttat aaaatttac 
gacgtggat tcgaccact tttgatttg ctcgactac 
gaagaatgg acgtttaat ctcacgtta gccaactct 
aaaagttcg aatgagtct cccgattgg tacaaattg 
tattctttt actgaagct ttcggagtt gataattta 
tcgccagtt gaggttgat aaagttttg tataaaatg 
gcagagaat cactcatta cttgacgat tactttttg 
ttcaaattt cgaaatggt gatgctgga attaaggat 
ggctgtgat gacaagtgc aagaaagat aatctgtgt 
gagattgca acaactgtg tatggagtg gacgtccac 
tgtaatcgt ttcactgat ctgtatgac aaaggacaa 
taa
    mRNA sequence
    • >TC032906 RA
      cgacttacc tacatccat tcattacga tggcgttcg 
cccagttag tttctgctt taattttga gttcgtttt 
tcttaacag taacaccaa ccgaagttc cagaatcgg 
tcacaaatg aaatcaagg aattattca aaacgggac 
aggctccca aaaatcttg ccaaagatt tggaaaatt 
tccgaatgc ctcgcttgt tccgcgaag gcatggaaa 
atacctacg aacaccctg tgatttgct tcttttgca 
atttgttgg tcgatttgg ctatcggtg agcgccggt 
tgggggcaa gtcgtgaag ttctagcca aagaaattg 
agtttattt gtgttcatc tgggtattg agagcgagc 
gcgtttgca cgggagtaa ttgatctga acatagacg 
tttttcttt atatcgttg acaattacc caaatcttg 
actcgcatc gagtttgtg ggtccgttc ttcagtctt 
tgggttgct cacctgggg ataattttg aatggtcaa 
ttgaactcc ctagtggta gttcgccag ttagaccta 
aacccaacg atacgctgg atagtttca aaattttgc 
aattgtctg atattcact atgacccga attataaac 
cgtatggaa atgctgatt gtggtgaac ccatctgtt 
gtcaaactg accaagggg agcctagtt cacccgaaa 
atacatgcg ggtattgga cggattacc gcgaggctg 
atgtgccgt ggcatttga tcgaagaaa cagtcagac 
aggccaaaa ctcaggagt tcgagtacg tttactaca 
ccggtgata tcatcagtc accgggttt gggaaacca 
gcattcgga ataactcgc aatcaatct ccgatcttt 
tcacctact ttaaacaaa gtttcgacg tcccagttt 
acccagttt ttggaaacc acgaacctc atccgcttg 
acctctggc ccactgaaa gcgtcaaag acgagaaat 
tctcagtcc agtggctct ttgagctag tttcaggcc 
cttggtctg aactcgtag gccaagata taagcgaaa 
ctgttctaa aagggggtt actatacag ttagtccaa 
aacccggat ttcgaatta tcgcaatta atggcaatc 
tttgttaca cctacaact ggtggctca ttttcaacg 
atgtcgacc cttacggac agttgcaat ggctgactg 
aaacgcttc ttgaagccg agaaaaaca acgaaagcg 
tccacattt tatcgcacg ttccgtcag gaacgtctg 
aaagtttat cagtttggg ggcgcgaat attccagaa 
ttattgaaa gattcgcaa ataccatca ctggccagt 
tcaatgggc acacacaca gggaccagt ttcacgttt 
attataaca gttcaaacc caacacaac caataggca 
ctgctttta acggggctt cggtaacac cttacaccg 
cctctaacc ccagttata aaatttacg acgtggatt 
cgaccactt ttgatttgc tcgactacg aagaatgga 
cgtttaatc tcacgttag ccaactcta aaagttcga 
atgagtctc ccgattggt acaaattgt attctttta 
ctgaagctt tcggagttg ataatttat cgccagttg 
aggttgata aagttttgt ataaaatgg cagagaatc 
actcattac ttgacgatt actttttgt tcaaatttc 
gaaatggtg atgctggaa ttaaggatg gctgtgatg 
acaagtgca agaaagata atctgtgtg agattgcaa 
caactgtgt atggagtgg acgtccact gtaatcgtt 
tcactgatc tgtatgaca aaggacaat aatgtttaa 
tttatttga tgaacttgg tacctgaat aaaatgctt 
gaaccatt