| General information | |||
| TC032906
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 11297832..11303801 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032906 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01112 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC032906 RA
MAFAQLVSA LILSSFFLT VTPTEVPES VTNEIKELF KTGQAPKNL AKDLENFRM PRLFREGME NTYEHPVIC FFCNLLVDL AIGERRLGA SREVLAKEI EFICVHLGI ESERVCTGV IDLNIDVFL YIVDNYPNL DSHRVCGSV LQSLGCSPG DNFEWSIEL PSGSSPVRP KPNDTLDSF KILQLSDIH YDPNYKPYG NADCGEPIC CQTDQGEPS SPENTCGYW TDYREADVP WHLIEETVR QAKTQEFEY VYYTGDIIS HRVWETSIR NNSQSISDL FTYFKQSFD VPVYPVFGN HEPHPLDLW PTESVKDEK FSVQWLFEL VSGPWSELV GQDISETVL KGGYYTVSP KPGFRIIAI NGNLCYTYN WWLIFNDVD PYGQLQWLT ETLLEAEKN NESVHILSH VPSGTSESL SVWGREYSR IIERFANTI TGQFNGHTH RDQFHVYYN SSNPTQPIG TAFNGASVT PYTASNPSY KIYDVDSTT FDLLDYEEW TFNLTLANS KSSNESPDW YKLYSFTEA FGVDNLSPV EVDKVLYKM AENHSLLDD YFLFKFRNG DAGIKDGCD DKCKKDNLC EIATTVYGV DVHCNRFTD LYDKGQ
- >TC032906 RA
atggcgttc gcccagtta gtttctgct ttaattttg agttcgttt ttcttaaca gtaacacca accgaagtt ccagaatcg gtcacaaat gaaatcaag gaattattc aaaacggga caggctccc aaaaatctt gccaaagat ttggaaaat ttccgaatg cctcgcttg ttccgcgaa ggcatggaa aatacctac gaacaccct gtgatttgc ttcttttgc aatttgttg gtcgatttg gctatcggt gagcgccgg ttgggggca agtcgtgaa gttctagcc aaagaaatt gagtttatt tgtgttcat ctgggtatt gagagcgag cgcgtttgc acgggagta attgatctg aacatagac gtttttctt tatatcgtt gacaattac ccaaatctt gactcgcat cgagtttgt gggtccgtt cttcagtct ttgggttgc tcacctggg gataatttt gaatggtca attgaactc cctagtggt agttcgcca gttagacct aaacccaac gatacgctg gatagtttc aaaattttg caattgtct gatattcac tatgacccg aattataaa ccgtatgga aatgctgat tgtggtgaa cccatctgt tgtcaaact gaccaaggg gagcctagt tcacccgaa aatacatgc gggtattgg acggattac cgcgaggct gatgtgccg tggcatttg atcgaagaa acagtcaga caggccaaa actcaggag ttcgagtac gtttactac accggtgat atcatcagt caccgggtt tgggaaacc agcattcgg aataactcg caatcaatc tccgatctt ttcacctac tttaaacaa agtttcgac gtcccagtt tacccagtt tttggaaac cacgaacct catccgctt gacctctgg cccactgaa agcgtcaaa gacgagaaa ttctcagtc cagtggctc tttgagcta gtttcaggc ccttggtct gaactcgta ggccaagat ataagcgaa actgttcta aaagggggt tactataca gttagtcca aaacccgga tttcgaatt atcgcaatt aatggcaat ctttgttac acctacaac tggtggctc attttcaac gatgtcgac ccttacgga cagttgcaa tggctgact gaaacgctt cttgaagcc gagaaaaac aacgaaagc gtccacatt ttatcgcac gttccgtca ggaacgtct gaaagttta tcagtttgg gggcgcgaa tattccaga attattgaa agattcgca aataccatc actggccag ttcaatggg cacacacac agggaccag tttcacgtt tattataac agttcaaac ccaacacaa ccaataggc actgctttt aacggggct tcggtaaca ccttacacc gcctctaac cccagttat aaaatttac gacgtggat tcgaccact tttgatttg ctcgactac gaagaatgg acgtttaat ctcacgtta gccaactct aaaagttcg aatgagtct cccgattgg tacaaattg tattctttt actgaagct ttcggagtt gataattta tcgccagtt gaggttgat aaagttttg tataaaatg gcagagaat cactcatta cttgacgat tactttttg ttcaaattt cgaaatggt gatgctgga attaaggat ggctgtgat gacaagtgc aagaaagat aatctgtgt gagattgca acaactgtg tatggagtg gacgtccac tgtaatcgt ttcactgat ctgtatgac aaaggacaa taa
- >TC032906 RA
cgacttacc tacatccat tcattacga tggcgttcg cccagttag tttctgctt taattttga gttcgtttt tcttaacag taacaccaa ccgaagttc cagaatcgg tcacaaatg aaatcaagg aattattca aaacgggac aggctccca aaaatcttg ccaaagatt tggaaaatt tccgaatgc ctcgcttgt tccgcgaag gcatggaaa atacctacg aacaccctg tgatttgct tcttttgca atttgttgg tcgatttgg ctatcggtg agcgccggt tgggggcaa gtcgtgaag ttctagcca aagaaattg agtttattt gtgttcatc tgggtattg agagcgagc gcgtttgca cgggagtaa ttgatctga acatagacg tttttcttt atatcgttg acaattacc caaatcttg actcgcatc gagtttgtg ggtccgttc ttcagtctt tgggttgct cacctgggg ataattttg aatggtcaa ttgaactcc ctagtggta gttcgccag ttagaccta aacccaacg atacgctgg atagtttca aaattttgc aattgtctg atattcact atgacccga attataaac cgtatggaa atgctgatt gtggtgaac ccatctgtt gtcaaactg accaagggg agcctagtt cacccgaaa atacatgcg ggtattgga cggattacc gcgaggctg atgtgccgt ggcatttga tcgaagaaa cagtcagac aggccaaaa ctcaggagt tcgagtacg tttactaca ccggtgata tcatcagtc accgggttt gggaaacca gcattcgga ataactcgc aatcaatct ccgatcttt tcacctact ttaaacaaa gtttcgacg tcccagttt acccagttt ttggaaacc acgaacctc atccgcttg acctctggc ccactgaaa gcgtcaaag acgagaaat tctcagtcc agtggctct ttgagctag tttcaggcc cttggtctg aactcgtag gccaagata taagcgaaa ctgttctaa aagggggtt actatacag ttagtccaa aacccggat ttcgaatta tcgcaatta atggcaatc tttgttaca cctacaact ggtggctca ttttcaacg atgtcgacc cttacggac agttgcaat ggctgactg aaacgcttc ttgaagccg agaaaaaca acgaaagcg tccacattt tatcgcacg ttccgtcag gaacgtctg aaagtttat cagtttggg ggcgcgaat attccagaa ttattgaaa gattcgcaa ataccatca ctggccagt tcaatgggc acacacaca gggaccagt ttcacgttt attataaca gttcaaacc caacacaac caataggca ctgctttta acggggctt cggtaacac cttacaccg cctctaacc ccagttata aaatttacg acgtggatt cgaccactt ttgatttgc tcgactacg aagaatgga cgtttaatc tcacgttag ccaactcta aaagttcga atgagtctc ccgattggt acaaattgt attctttta ctgaagctt tcggagttg ataatttat cgccagttg aggttgata aagttttgt ataaaatgg cagagaatc actcattac ttgacgatt actttttgt tcaaatttc gaaatggtg atgctggaa ttaaggatg gctgtgatg acaagtgca agaaagata atctgtgtg agattgcaa caactgtgt atggagtgg acgtccact gtaatcgtt tcactgatc tgtatgaca aaggacaat aatgtttaa tttatttga tgaacttgg tacctgaat aaaatgctt gaaccatt
