Status
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Gene information
General information
TC007010 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12009532..12024231
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007010
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01108

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007010 RA
      MEEISDLSN KKLKKLAKF SPQESQVIT TLILDDNEL 
      QRLDNIDSL FRLQKLSAV RNQLLRMYG VCRLHSLHT 
      LNLAHNGIL TIEGLKDLV NLKWLCLAG NSIKTIEHL 
      NTNTNLEHL DLSENNITH INDLAFLKD LKELFLHNN 
      KINHLRQSD RFLPTSLIT LTLANNNIE DLNEISQLV 
      HLVNLQNIS IANNPCVNF GNTNIGFDY RPFVINWCL 
      NIKTIDGYV VDAIESLRA EWLYSQGRG RHFRVGDQK 
      DLVQYLASV CPLTGEALE TEEDRKLRL ILSKAQHHQ 
      QQLREQSTG SSNPSPLTR KKLHGAKHS PRLNSARAT 
      AKLRSPDRM SSSCYGNAN VMENSNIMS QSLDPTILR 
      QSVTPSKTP SEKRLDDEP VNSPLQALS KMVPVPESL 
      MSPDYRPAP ILGKTVLTS VNNKKLPQA VRSPKLGRN 
      LHNKNKPQA NDGGKSSSP SPARARKAM NHENNANKT 
      SILRKSLSS DDESEVFVS KLQKIQIRA EEKRIMNQK 
      DAEILNNCN SEDRVEQAA VFIQKMWRG YFTRNRNKE 
      VQEIFRTLQ AQRANQYIQ QLTDDMETT KAALESERK 
      IQMLQTEAI SALWKQMSA LQPNSGQSS TANMTNLST 
      DNVEVVKEL AKTCTVLQN QIQQLQSSM QDIVKVMTV 
      FSQTTSINP NGIATQTEI VAVHTPQGE AAKVFPFHK 
      QTRPSSLPL PINQRKKDN VNTELQQYA DNLVDGVIK 
      TVSETRADC VGSDLTEIT VTDTAKMDE TQVSN
    CDS sequence
    • >TC007010 RA
      atggaggaa atatcagat ttgtcgaac aagaaactc 
      aaaaaactc gccaaattc tcgcctcag gaatcgcaa 
      gtgataaca acattgata ctcgacgat aacgaactg 
      caacgattg gataacatc gactcgttg tttcggcta 
      cagaagctg tcagcagtg cgcaatcaa ttgttgcgc 
      atgtatggc gtatgtcgc ctccattct ctgcacact 
      ttgaatctc gctcataac ggaatactc acaatcgag 
      gggctcaag gatctggtt aatttaaag tggctttgc 
      ttggctggg aatagtata aaaactatt gaacatctc 
      aatactaac acgaattta gagcattta gatttgtcg 
      gagaataac attacgcac atcaatgat ttagcattc 
      ttgaaagat ttaaaggag ctttttcta cacaataac 
      aaaattaat catcttaga cagagtgat cgtttcctg 
      cctactagt ttaataacc ctgactttg gctaataat 
      aacattgag gatttgaat gaaatttct cagcttgtt 
      catcttgtt aatctacaa aatatttcc atcgctaat 
      aacccttgt gttaacttt ggcaacact aacattgga 
      tttgattac cgtccgttt gtgatcaac tggtgtttg 
      aacattaaa acaattgat ggttatgta gtggatgct 
      attgaaagt ttaagagct gagtggttg tattcacaa 
      ggtaggggt cgtcatttt cgagttggc gatcaaaaa 
      gatcttgtt cagtattta gcaagcgtt tgtccactc 
      actggtgaa gctcttgaa acagaagag gatcgaaaa 
      ctacgttta attttgagt aaagcacaa catcaccaa 
      caacaatta agggaacaa tctactgga tcttcaaat 
      ccttcacct ctgacaagg aaaaagttg catggagcg 
      aaacattcg ccaaggtta aattctgct cgagctact 
      gctaagtta agatcacca gatcgcatg tcttcaagc 
      tgttatgga aatgcaaac gttatggaa aattcaaat 
      attatgagt caaagttta gatccaaca attttaagg 
      caaagtgtt actccgtca aaaacacca agcgaaaaa 
      cgtttagat gatgagcct gttaatagt ccccttcaa 
      gctctttca aagatggtt ccagtacca gaatctcta 
      atgagtcca gattaccgt ccagctcca attcttggt 
      aaaactgtc ttaacttca gtcaataat aaaaaatta 
      cctcaagct gtgcgatca cctaaactt ggtcgaaat 
      ttacataac aagaataaa ccacaagct aatgatgga 
      ggaaagagc agttcacct tcgcctgct agagcaagg 
      aaagcaatg aatcatgaa aataatgcc aataagact 
      tcaattttg cgaaagtct ctaagtagt gatgacgag 
      tcggaagta tttgtttca aaattgcaa aaaattcaa 
      ataagagcc gaagaaaaa cgaataatg aatcaaaaa 
      gacgccgaa attttaaat aattgcaac agcgaagac 
      cgagttgaa caagcagct gtttttata caaaaaatg 
      tggcgagga tactttacc cgaaacaga aacaaagaa 
      gtacaagaa atttttcga acattacaa gcacaaaga 
      gcaaatcaa tacatacaa caattgact gacgatatg 
      gaaacaact aaagctgca ttagaaagt gaacgaaaa 
      attcaaatg ttacaaact gaagcaata agcgccctc 
      tggaagcaa atgtcagct ttacaacca aatagtggc 
      caatcaagt actgccaat atgacaaat ttatcgaca 
      gataatgta gaagttgta aaagagttg gccaaaacg 
      tgtactgtt ttgcaaaat caaattcaa caattacaa 
      agttcaatg caagatatt gtcaaagtt atgactgtt 
      tttagtcaa acgacttca attaatccc aatggaatt 
      gcgactcaa actgaaatt gtggctgta catacgcca 
      caaggggaa gctgcaaaa gtttttccc tttcacaaa 
      caaacccgg ccatcttct cttccactg ccgatcaac 
      caacgcaaa aaagacaac gtcaacacg gaattgcaa 
      cagtacgct gataatcta gtagatggt gttattaaa 
      acagtgtcc gaaacaaga gctgattgt gttggaagt 
      gacctgacc gaaataaca gtcaccgat acagcgaaa 
      atggacgaa acacaagta tcaaactga
    mRNA sequence
    • >TC007010 RA
      tgatcgtta ttgtttata cggaatata atccaacac 
      atgcactta cattttcta accaatatc gtaaacttc 
      tataagtga aaggaacac aacgattcc tgctcacaa 
      ttttaaaat gtggaacaa tttaataat cagctgatc 
      atcgccgtc cttctatta gcgccatca cctacctgt 
      caataatat ttagcaaat aaaaaaatg cgtataagt 
      taaatataa atggaggaa atatcagat ttgtcgaac 
      aagaaactc aaaaaactc gccaaattc tcgcctcag 
      gaatcgcaa gtgataaca acattgata ctcgacgat 
      aacgaactg caacgattg gataacatc gactcgttg 
      tttcggcta cagaagctg tcagcagtg cgcaatcaa 
      ttgttgcgc atgtatggc gtatgtcgc ctccattct 
      ctgcacact ttgaatctc gctcataac ggaatactc 
      acaatcgag gggctcaag gatctggtt aatttaaag 
      tggctttgc ttggctggg aatagtata aaaactatt 
      gaacatctc aatactaac acgaattta gagcattta 
      gatttgtcg gagaataac attacgcac atcaatgat 
      ttagcattc ttgaaagat ttaaaggag ctttttcta 
      cacaataac aaaattaat catcttaga cagagtgat 
      cgtttcctg cctactagt ttaataacc ctgactttg 
      gctaataat aacattgag gatttgaat gaaatttct 
      cagcttgtt catcttgtt aatctacaa aatatttcc 
      atcgctaat aacccttgt gttaacttt ggcaacact 
      aacattgga tttgattac cgtccgttt gtgatcaac 
      tggtgtttg aacattaaa acaattgat ggttatgta 
      gtggatgct attgaaagt ttaagagct gagtggttg 
      tattcacaa ggtaggggt cgtcatttt cgagttggc 
      gatcaaaaa gatcttgtt cagtattta gcaagcgtt 
      tgtccactc actggtgaa gctcttgaa acagaagag 
      gatcgaaaa ctacgttta attttgagt aaagcacaa 
      catcaccaa caacaatta agggaacaa tctactgga 
      tcttcaaat ccttcacct ctgacaagg aaaaagttg 
      catggagcg aaacattcg ccaaggtta aattctgct 
      cgagctact gctaagtta agatcacca gatcgcatg 
      tcttcaagc tgttatgga aatgcaaac gttatggaa 
      aattcaaat attatgagt caaagttta gatccaaca 
      attttaagg caaagtgtt actccgtca aaaacacca 
      agcgaaaaa cgtttagat gatgagcct gttaatagt 
      ccccttcaa gctctttca aagatggtt ccagtacca 
      gaatctcta atgagtcca gattaccgt ccagctcca 
      attcttggt aaaactgtc ttaacttca gtcaataat 
      aaaaaatta cctcaagct gtgcgatca cctaaactt 
      ggtcgaaat ttacataac aagaataaa ccacaagct 
      aatgatgga ggaaagagc agttcacct tcgcctgct 
      agagcaagg aaagcaatg aatcatgaa aataatgcc 
      aataagact tcaattttg cgaaagtct ctaagtagt 
      gatgacgag tcggaagta tttgtttca aaattgcaa 
      aaaattcaa ataagagcc gaagaaaaa cgaataatg 
      aatcaaaaa gacgccgaa attttaaat aattgcaac 
      agcgaagac cgagttgaa caagcagct gtttttata 
      caaaaaatg tggcgagga tactttacc cgaaacaga 
      aacaaagaa gtacaagaa atttttcga acattacaa 
      gcacaaaga gcaaatcaa tacatacaa caattgact 
      gacgatatg gaaacaact aaagctgca ttagaaagt 
      gaacgaaaa attcaaatg ttacaaact gaagcaata 
      agcgccctc tggaagcaa atgtcagct ttacaacca 
      aatagtggc caatcaagt actgccaat atgacaaat 
      ttatcgaca gataatgta gaagttgta aaagagttg 
      gccaaaacg tgtactgtt ttgcaaaat caaattcaa 
      caattacaa agttcaatg caagatatt gtcaaagtt 
      atgactgtt tttagtcaa acgacttca attaatccc 
      aatggaatt gcgactcaa actgaaatt gtggctgta 
      catacgcca caaggggaa gctgcaaaa gtttttccc 
      tttcacaaa caaacccgg ccatcttct cttccactg 
      ccgatcaac caacgcaaa aaagacaac gtcaacacg 
      gaattgcaa cagtacgct gataatcta gtagatggt 
      gttattaaa acagtgtcc gaaacaaga gctgattgt 
      gttggaagt gacctgacc gaaataaca gtcaccgat 
      acagcgaaa atggacgaa acacaagta tcaaactga 
      aaatacatt aagtactaa ttttattta agtgacaga 
      gttgttgtc gtgtgtact acagaggtt tgacacacg 
      ctgtattta aaaatcagt gtgacgcaa attgttttt 
      gtaccaaat ataatttta ttattatac acagacacg 
      tttagattg gagcttttc ttgtgtgca tacaaagta 
      caaattgtc tttttgaaa gaataccga aatgtaatg 
      attgtggta ctctctcaa atgttcaag acatttcga 
      ggtccgcac ctacattaa aaaattcaa tttttaagc 
      tttaataaa tgttgaaac atcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY