| General information | |||
| TC007009 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 12052137..12067075 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007009 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01107 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007009 RA
MYQNACGAA AASAGGCVG TERDCHSLI SKEPPVPAI EHDYSGFDI VKATQYGAF GRVKELVEA GYNVNQRDS ETVTLLHWA AINNRKDII RYFIEKGAE VDAVGGELN ATPLHWATR QGHLDAVVI LMNAGTDPT LRDAEGCSC IHLAAQFGH TALVAYFIA RGISPDLVD RAGMTPLMW SCWKVCSLD PTRLLLTLG ASPNLTDQQ GNTALHWAI LARNTSAIS TLIVQGQAS LNVPNHRGD TPLSMLQTQ LGAIWIGSK VAERIKEAI SSGHTRNIF TKITKDKRL RWWCMVGTP FMAFYIAGS ILGTNLIIL IKLFLLVCL YIVLHYAGQ ILYDDRLMA LLPLSIYLS TKLWMYFTW FIYIMPATN IITTIVFLS SSGILWYCF LKSWLGDAG VISTSQQLR FRTIIELAE QGSGGFEPS TFCSSCLVR RPLRSKHCS VCNKCVARF DHHCPWVAN CIGAKNHKY FIGFLASLV MMCCQMLYG SVKFWQNQT TCNVTTSEG YWKFVLSIA QCDTWVAWV AANTLFHCV WVFTLFVCQ LYQISCLGM TTNERMNRG RYSYIVSNG GKSPFSKGP IKNILEFFD CTCFGVFKP ENKDWLTSY DLDKNIEHQ PLLKNKENF QYV
- >TC007009 RA
atgtaccaa aacgcttgc ggggcagct gctgcgagt gccggcggt tgtgttggc accgaacgt gattgccac agtttaata tccaaagaa ccaccagtc ccagcaatc gagcacgac tacagcggt ttcgacatc gtcaaagcc actcaatat ggtgccttt ggccgcgtc aaagaacta gtcgaagcc ggctataac gtcaaccag cgagacagc gaaacagtg actttgctc cactgggcg gctatcaac aaccgcaaa gacattatt cgttatttt atcgagaag ggagctgaa gtggacgct gtggggggc gagttaaat gcaaccccc cttcactgg gccacccgc caaggccac ctcgatgcc gtggtgatt ttaatgaac gctggcaca gatccgacg ctcagagac gctgagggt tgttcttgc atacattta gcagcgcag tttggacac acggccctt gtggcgtat tttatcgca agggggatt agtcccgat ttggtggat agggccggc atgacgcct ttgatgtgg tcgtgttgg aaagtttgt agtttggat ccgactaga ttgttgttg acgttaggg gcatccccg aatttgaca gatcagcaa gggaataca gcactacat tgggcgatt ttggctagg aatacgtcg gctatttca acgttgatt gttcaggga caagcaagt ctaaatgta cctaatcat aggggagat acaccactg tcgatgtta caaacacag ttaggggct atttggata ggttccaaa gttgcggaa cggattaaa gaagcgata agtagtggg catacaaga aatattttt acgaaaatt actaaagat aagagatta cgctggtgg tgcatggta ggcaccccc tttatggca ttttacatc gctggttcg attttaggc acaaattta ataatttta atcaagcta tttctatta gtgtgctta tacatagtt ttgcattat gccggtcaa attttatac gacgataga ttaatggca cttctacct cttagtatt tatttatca acaaaatta tggatgtat ttcacatgg tttatttat ataatgcca gccacaaac attataaca acaatcgtc ttcctatct agttcagga atattatgg tattgcttt ctcaagtca tggttaggt gatgctggt gttatttca acttcacaa cagctgaga tttaggact ataattgaa ttggccgaa caaggttca ggtggtttc gaaccgtcg actttttgt tcgtcttgt ttagtgaga agaccgtta agatctaag cattgctca gtgtgcaat aaatgtgtc gcaaggttt gatcatcac tgtccgtgg gttgctaat tgtataggt gctaaaaat cacaaatat ttcatcggt tttttggcc agtttagta atgatgtgt tgtcaaatg ttgtacgga tctgtgaaa ttttggcaa aaccaaact acttgtaac gtcacaaca tctgaaggt tattggaaa tttgtttta tcaattgcg cagtgtgac acgtgggtt gcgtgggtt gctgccaat actttattc cattgtgtt tgggtcttc acattgttt gtttgtcaa ctttatcag atttcgtgt cttggtatg acaacgaac gaaagaatg aacagaggg cgctacagt tacattgta agtaatggc gggaaaagt ccattttca aaaggtccg ataaaaaat attcttgaa tttttcgat tgcacttgt ttcggtgtt tttaaaccg gaaaataaa gactggttg acgagttac gatttagat aagaatatc gaacatcaa ccacttttg aaaaacaaa gagaatttt caatatgtt taa
- >TC007009 RA
atacttcta atatggggt ttagacgga acagcctgt ataagtttt gacatgacc agagtcaca gcagttgtt atgacttga cactgacag caatgtgaa atgtcaagt ggagcgttt gcagatttg accgccaga gcgcgctgc aattttaat accacgatt taactaggt tttattata tatcatgtt ttggtaatc actcggcgc atctatatt tcatactgt tgttgttgg gtacttaag aatatgttg caaacttgt cttttttta atatgttta atatgttga aaggtatta tacgtattt agttggagt ttagtgact gagacgctc aaattttta ttttagtaa catttttat gttttaaaa gtgactttg aaatattat aaatatgta atgccaatg aaatttgac ttagaaaac cactccatt ccttgttct aacagattt agaaaagaa tagaaaagt tagtgctaa ttaggtaag tttaatttt taatttgta gtttttaac actatcagc agtgaaagt aagtgaact gtttttgtt ttggtttct agttttttt caattttag tagtaataa tcaggtatg ttcacagtc tctttcaag tagataaga gaagttatt tctagataa ctaacaagt tttaagaac ttgttggtt tcttagact caatattga tatatgaaa aaagttata aataataaa aaagagtat attgagata tccattaaa tgacgtttc aagggtata atttccctt ttcttcaga ttaaacaag tttaataca aagactttg acacacttg agtgtatct ttctagttt taacttctt ttttaatat tggatagtt tcgagtttc ttcaatgtt attattgta cgtattttc atattttct gtttgattt ttacatttg caaatattt ttctctcac taagggccg cttttacaa tcatccgat aaactccgg ataagttac ctgtgttag aaatttata ttaggaata acaggccga catccgtca gataatttt tttgtcttt tacaataca agcaattta tccatggaa taaatgtga catttgagc attattgtg acagttagt ttttaacta aataatgca agaagttaa cgtatttga atcagacag cgatggcaa attaattga gtatgtgga caggttaag agtacctac ttaaacaat gttatccaa aaaaatgtc cataaagtt ctcaaaaag caaacaacc aatgagaac agagtataa atttatcct atagtttat ccggtcgtc gaacgacca gataattta ttacgtagt ttataccgt caattatcc tatcagggg atcatcgga cgattggaa aactgtttt tttaattta ttctaggga taaagttat cgagagttt atctggcga tcgtaaaag cggccctaa gttgttttt ctgtttgtc ttgctttac ttccttttt tttctcatg tgttttttt aacttaaag gaaattatt gcagagagg taaagtttt ttgaccctg taaccgcta ttactttct ctcttttag aacataaac ataactgaa taaagttat agctaatga agttgtcaa caaaaagtt attataact tgtgctata atttaaaaa ttgtttgtt ggaattggt gttacaaaa ataatcaca aaattcatt tcgtagtga ttattgtgt tactgatta tacaggatc attcatttc aacgccaac gtcgcattt taccgaatt attttttta aataagatt gattaaatt ttcaaatag taatgaaaa aactaatta tatttactc tgaccgttt cacccatct taattgtac tcaaatatt gctaaaatg tgtaaatga tcattctaa ttaacggag agctattcg tctcgtcac gaattatcg atttccgcc agtccgaaa cctatattg ttcctatat tgaaaccta acctccatt tgcattaat gtttgtgtc attctcctg tcaaactta atgatcaaa aatttaaaa agatctatt aaaaatgta ccaaaacgc ttgcggggc agctgctgc gagtgccgg cggttgtgt tggcaccga acgtgattg ccacagttt aatatccaa agaaccacc agtcccagc aatcgagca cgactacag cggtttcga catcgtcaa agccactca atatggtgc ctttggccg cgtcaaaga actagtcga agccggcta taacgtcaa ccagcgaga cagcgaaac agtgacttt gctccactg ggcggctat caacaaccg caaagacat tattcgtta ttttatcga gaagggagc tgaagtgga cgctgtggg gggcgagtt aaatgcaac cccccttca ctgggccac ccgccaagg ccacctcga tgccgtggt gattttaat gaacgctgg cacagatcc gacgctcag agacgctga gggttgttc ttgcataca tttagcagc gcagtttgg acacacggc ccttgtggc gtattttat cgcaagggg gattagtcc cgatttggt ggatagggc cggcatgac gcctttgat gtggtcgtg ttggaaagt ttgtagttt ggatccgac tagattgtt gttgacgtt aggggcatc cccgaattt gacagatca gcaagggaa tacagcact acattgggc gattttggc taggaatac gtcggctat ttcaacgtt gattgttca gggacaagc aagtctaaa tgtacctaa tcatagggg agatacacc actgtcgat gttacaaac acagttagg ggctatttg gataggttc caaagttgc ggaacggat taaagaagc gataagtag tgggcatac aagaaatat ttttacgaa aattactaa agataagag attacgctg gtggtgcat ggtaggcac cccctttat ggcatttta catcgctgg ttcgatttt aggcacaaa tttaataat tttaatcaa gctatttct attagtgtg cttatacat agttttgca ttatgccgg tcaaatttt atacgacga tagattaat ggcacttct acctcttag tatttattt atcaacaaa attatggat gtatttcac atggtttat ttatataat gccagccac aaacattat aacaacaat cgtcttcct atctagttc aggaatatt atggtattg ctttctcaa gtcatggtt aggtgatgc tggtgttat ttcaacttc acaacagct gagatttag gactataat tgaattggc cgaacaagg ttcaggtgg tttcgaacc gtcgacttt ttgttcgtc ttgtttagt gagaagacc gttaagatc taagcattg ctcagtgtg caataaatg tgtcgcaag gtttgatca tcactgtcc gtgggttgc taattgtat aggtgctaa aaatcacaa atatttcat cggtttttt ggccagttt agtaatgat gtgttgtca aatgttgta cggatctgt gaaattttg gcaaaacca aactacttg taacgtcac aacatctga aggttattg gaaatttgt tttatcaat tgcgcagtg tgacacgtg ggttgcgtg ggttgctgc caatacttt attccattg tgtttgggt cttcacatt gtttgtttg tcaacttta tcagatttc gtgtcttgg tatgacaac gaacgaaag aatgaacag agggcgcta cagttacat tgtaagtaa tggcgggaa aagtccatt ttcaaaagg tccgataaa aaatattct tgaattttt cgattgcac ttgtttcgg tgtttttaa accggaaaa taaagactg gttgacgag ttacgattt agataagaa tatcgaaca tcaaccact tttgaaaaa caaagagaa ttttcaata tgtttaaat attgattta aaataataa tccaaagct atgactgag acattgaat gattgtcaa tttgtaaag ttagctctc taagcagaa acattcctt tttattttg cattgcttc cgtaaagtg tcatgtaaa tattttaat tgtaaatac gtagtttca ataggattc atttaattg atgcaaaaa gagatcaat atttttgcc tcaatttta tcaatctgc taaaagtat ttttcacta tatctagag acttagtaa aaaatactt taataaata atgattaac tacaataat tcttatcac gtacatgct gtagcattt tcaatctag tcaactttg gcatgtact tagataatg cgataaatg caccaaaat caagtgtca ttctaattc atacaattt cggtgtaaa cttgacatt tcttcacta aaaaatcac ttaaattgt tgctctaaa tcaaaaata tcacgtttt tttttaatt gagtagaca tttagctaa tatttagcc ttttactag tatgttctt tgcgcgtgt tggctttat gtgtaataa gctttaagc gaaaatgta gataacatc acgttttac agtaatgtt tataattaa acactactt gcattgtac attgtacct attatattt tgtatgtaa taattacat tacattcac
