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Gene information
General information
TC007009 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12052137..12067075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007009
    iBeetle screen iB_01107

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007009 RA
      MYQNACGAA AASAGGCVG TERDCHSLI SKEPPVPAI 
      EHDYSGFDI VKATQYGAF GRVKELVEA GYNVNQRDS 
      ETVTLLHWA AINNRKDII RYFIEKGAE VDAVGGELN 
      ATPLHWATR QGHLDAVVI LMNAGTDPT LRDAEGCSC 
      IHLAAQFGH TALVAYFIA RGISPDLVD RAGMTPLMW 
      SCWKVCSLD PTRLLLTLG ASPNLTDQQ GNTALHWAI 
      LARNTSAIS TLIVQGQAS LNVPNHRGD TPLSMLQTQ 
      LGAIWIGSK VAERIKEAI SSGHTRNIF TKITKDKRL 
      RWWCMVGTP FMAFYIAGS ILGTNLIIL IKLFLLVCL 
      YIVLHYAGQ ILYDDRLMA LLPLSIYLS TKLWMYFTW 
      FIYIMPATN IITTIVFLS SSGILWYCF LKSWLGDAG 
      VISTSQQLR FRTIIELAE QGSGGFEPS TFCSSCLVR 
      RPLRSKHCS VCNKCVARF DHHCPWVAN CIGAKNHKY 
      FIGFLASLV MMCCQMLYG SVKFWQNQT TCNVTTSEG 
      YWKFVLSIA QCDTWVAWV AANTLFHCV WVFTLFVCQ 
      LYQISCLGM TTNERMNRG RYSYIVSNG GKSPFSKGP 
      IKNILEFFD CTCFGVFKP ENKDWLTSY DLDKNIEHQ 
      PLLKNKENF QYV
    CDS sequence
    • >TC007009 RA
      atgtaccaa aacgcttgc ggggcagct gctgcgagt 
      gccggcggt tgtgttggc accgaacgt gattgccac 
      agtttaata tccaaagaa ccaccagtc ccagcaatc 
      gagcacgac tacagcggt ttcgacatc gtcaaagcc 
      actcaatat ggtgccttt ggccgcgtc aaagaacta 
      gtcgaagcc ggctataac gtcaaccag cgagacagc 
      gaaacagtg actttgctc cactgggcg gctatcaac 
      aaccgcaaa gacattatt cgttatttt atcgagaag 
      ggagctgaa gtggacgct gtggggggc gagttaaat 
      gcaaccccc cttcactgg gccacccgc caaggccac 
      ctcgatgcc gtggtgatt ttaatgaac gctggcaca 
      gatccgacg ctcagagac gctgagggt tgttcttgc 
      atacattta gcagcgcag tttggacac acggccctt 
      gtggcgtat tttatcgca agggggatt agtcccgat 
      ttggtggat agggccggc atgacgcct ttgatgtgg 
      tcgtgttgg aaagtttgt agtttggat ccgactaga 
      ttgttgttg acgttaggg gcatccccg aatttgaca 
      gatcagcaa gggaataca gcactacat tgggcgatt 
      ttggctagg aatacgtcg gctatttca acgttgatt 
      gttcaggga caagcaagt ctaaatgta cctaatcat 
      aggggagat acaccactg tcgatgtta caaacacag 
      ttaggggct atttggata ggttccaaa gttgcggaa 
      cggattaaa gaagcgata agtagtggg catacaaga 
      aatattttt acgaaaatt actaaagat aagagatta 
      cgctggtgg tgcatggta ggcaccccc tttatggca 
      ttttacatc gctggttcg attttaggc acaaattta 
      ataatttta atcaagcta tttctatta gtgtgctta 
      tacatagtt ttgcattat gccggtcaa attttatac 
      gacgataga ttaatggca cttctacct cttagtatt 
      tatttatca acaaaatta tggatgtat ttcacatgg 
      tttatttat ataatgcca gccacaaac attataaca 
      acaatcgtc ttcctatct agttcagga atattatgg 
      tattgcttt ctcaagtca tggttaggt gatgctggt 
      gttatttca acttcacaa cagctgaga tttaggact 
      ataattgaa ttggccgaa caaggttca ggtggtttc 
      gaaccgtcg actttttgt tcgtcttgt ttagtgaga 
      agaccgtta agatctaag cattgctca gtgtgcaat 
      aaatgtgtc gcaaggttt gatcatcac tgtccgtgg 
      gttgctaat tgtataggt gctaaaaat cacaaatat 
      ttcatcggt tttttggcc agtttagta atgatgtgt 
      tgtcaaatg ttgtacgga tctgtgaaa ttttggcaa 
      aaccaaact acttgtaac gtcacaaca tctgaaggt 
      tattggaaa tttgtttta tcaattgcg cagtgtgac 
      acgtgggtt gcgtgggtt gctgccaat actttattc 
      cattgtgtt tgggtcttc acattgttt gtttgtcaa 
      ctttatcag atttcgtgt cttggtatg acaacgaac 
      gaaagaatg aacagaggg cgctacagt tacattgta 
      agtaatggc gggaaaagt ccattttca aaaggtccg 
      ataaaaaat attcttgaa tttttcgat tgcacttgt 
      ttcggtgtt tttaaaccg gaaaataaa gactggttg 
      acgagttac gatttagat aagaatatc gaacatcaa 
      ccacttttg aaaaacaaa gagaatttt caatatgtt 
      taa
    mRNA sequence
    • >TC007009 RA
      atacttcta atatggggt ttagacgga acagcctgt 
      ataagtttt gacatgacc agagtcaca gcagttgtt 
      atgacttga cactgacag caatgtgaa atgtcaagt 
      ggagcgttt gcagatttg accgccaga gcgcgctgc 
      aattttaat accacgatt taactaggt tttattata 
      tatcatgtt ttggtaatc actcggcgc atctatatt 
      tcatactgt tgttgttgg gtacttaag aatatgttg 
      caaacttgt cttttttta atatgttta atatgttga 
      aaggtatta tacgtattt agttggagt ttagtgact 
      gagacgctc aaattttta ttttagtaa catttttat 
      gttttaaaa gtgactttg aaatattat aaatatgta 
      atgccaatg aaatttgac ttagaaaac cactccatt 
      ccttgttct aacagattt agaaaagaa tagaaaagt 
      tagtgctaa ttaggtaag tttaatttt taatttgta 
      gtttttaac actatcagc agtgaaagt aagtgaact 
      gtttttgtt ttggtttct agttttttt caattttag 
      tagtaataa tcaggtatg ttcacagtc tctttcaag 
      tagataaga gaagttatt tctagataa ctaacaagt 
      tttaagaac ttgttggtt tcttagact caatattga 
      tatatgaaa aaagttata aataataaa aaagagtat 
      attgagata tccattaaa tgacgtttc aagggtata 
      atttccctt ttcttcaga ttaaacaag tttaataca 
      aagactttg acacacttg agtgtatct ttctagttt 
      taacttctt ttttaatat tggatagtt tcgagtttc 
      ttcaatgtt attattgta cgtattttc atattttct 
      gtttgattt ttacatttg caaatattt ttctctcac 
      taagggccg cttttacaa tcatccgat aaactccgg 
      ataagttac ctgtgttag aaatttata ttaggaata 
      acaggccga catccgtca gataatttt tttgtcttt 
      tacaataca agcaattta tccatggaa taaatgtga 
      catttgagc attattgtg acagttagt ttttaacta 
      aataatgca agaagttaa cgtatttga atcagacag 
      cgatggcaa attaattga gtatgtgga caggttaag 
      agtacctac ttaaacaat gttatccaa aaaaatgtc 
      cataaagtt ctcaaaaag caaacaacc aatgagaac 
      agagtataa atttatcct atagtttat ccggtcgtc 
      gaacgacca gataattta ttacgtagt ttataccgt 
      caattatcc tatcagggg atcatcgga cgattggaa 
      aactgtttt tttaattta ttctaggga taaagttat 
      cgagagttt atctggcga tcgtaaaag cggccctaa 
      gttgttttt ctgtttgtc ttgctttac ttccttttt 
      tttctcatg tgttttttt aacttaaag gaaattatt 
      gcagagagg taaagtttt ttgaccctg taaccgcta 
      ttactttct ctcttttag aacataaac ataactgaa 
      taaagttat agctaatga agttgtcaa caaaaagtt 
      attataact tgtgctata atttaaaaa ttgtttgtt 
      ggaattggt gttacaaaa ataatcaca aaattcatt 
      tcgtagtga ttattgtgt tactgatta tacaggatc 
      attcatttc aacgccaac gtcgcattt taccgaatt 
      attttttta aataagatt gattaaatt ttcaaatag 
      taatgaaaa aactaatta tatttactc tgaccgttt 
      cacccatct taattgtac tcaaatatt gctaaaatg 
      tgtaaatga tcattctaa ttaacggag agctattcg 
      tctcgtcac gaattatcg atttccgcc agtccgaaa 
      cctatattg ttcctatat tgaaaccta acctccatt 
      tgcattaat gtttgtgtc attctcctg tcaaactta 
      atgatcaaa aatttaaaa agatctatt aaaaatgta 
      ccaaaacgc ttgcggggc agctgctgc gagtgccgg 
      cggttgtgt tggcaccga acgtgattg ccacagttt 
      aatatccaa agaaccacc agtcccagc aatcgagca 
      cgactacag cggtttcga catcgtcaa agccactca 
      atatggtgc ctttggccg cgtcaaaga actagtcga 
      agccggcta taacgtcaa ccagcgaga cagcgaaac 
      agtgacttt gctccactg ggcggctat caacaaccg 
      caaagacat tattcgtta ttttatcga gaagggagc 
      tgaagtgga cgctgtggg gggcgagtt aaatgcaac 
      cccccttca ctgggccac ccgccaagg ccacctcga 
      tgccgtggt gattttaat gaacgctgg cacagatcc 
      gacgctcag agacgctga gggttgttc ttgcataca 
      tttagcagc gcagtttgg acacacggc ccttgtggc 
      gtattttat cgcaagggg gattagtcc cgatttggt 
      ggatagggc cggcatgac gcctttgat gtggtcgtg 
      ttggaaagt ttgtagttt ggatccgac tagattgtt 
      gttgacgtt aggggcatc cccgaattt gacagatca 
      gcaagggaa tacagcact acattgggc gattttggc 
      taggaatac gtcggctat ttcaacgtt gattgttca 
      gggacaagc aagtctaaa tgtacctaa tcatagggg 
      agatacacc actgtcgat gttacaaac acagttagg 
      ggctatttg gataggttc caaagttgc ggaacggat 
      taaagaagc gataagtag tgggcatac aagaaatat 
      ttttacgaa aattactaa agataagag attacgctg 
      gtggtgcat ggtaggcac cccctttat ggcatttta 
      catcgctgg ttcgatttt aggcacaaa tttaataat 
      tttaatcaa gctatttct attagtgtg cttatacat 
      agttttgca ttatgccgg tcaaatttt atacgacga 
      tagattaat ggcacttct acctcttag tatttattt 
      atcaacaaa attatggat gtatttcac atggtttat 
      ttatataat gccagccac aaacattat aacaacaat 
      cgtcttcct atctagttc aggaatatt atggtattg 
      ctttctcaa gtcatggtt aggtgatgc tggtgttat 
      ttcaacttc acaacagct gagatttag gactataat 
      tgaattggc cgaacaagg ttcaggtgg tttcgaacc 
      gtcgacttt ttgttcgtc ttgtttagt gagaagacc 
      gttaagatc taagcattg ctcagtgtg caataaatg 
      tgtcgcaag gtttgatca tcactgtcc gtgggttgc 
      taattgtat aggtgctaa aaatcacaa atatttcat 
      cggtttttt ggccagttt agtaatgat gtgttgtca 
      aatgttgta cggatctgt gaaattttg gcaaaacca 
      aactacttg taacgtcac aacatctga aggttattg 
      gaaatttgt tttatcaat tgcgcagtg tgacacgtg 
      ggttgcgtg ggttgctgc caatacttt attccattg 
      tgtttgggt cttcacatt gtttgtttg tcaacttta 
      tcagatttc gtgtcttgg tatgacaac gaacgaaag 
      aatgaacag agggcgcta cagttacat tgtaagtaa 
      tggcgggaa aagtccatt ttcaaaagg tccgataaa 
      aaatattct tgaattttt cgattgcac ttgtttcgg 
      tgtttttaa accggaaaa taaagactg gttgacgag 
      ttacgattt agataagaa tatcgaaca tcaaccact 
      tttgaaaaa caaagagaa ttttcaata tgtttaaat 
      attgattta aaataataa tccaaagct atgactgag 
      acattgaat gattgtcaa tttgtaaag ttagctctc 
      taagcagaa acattcctt tttattttg cattgcttc 
      cgtaaagtg tcatgtaaa tattttaat tgtaaatac 
      gtagtttca ataggattc atttaattg atgcaaaaa 
      gagatcaat atttttgcc tcaatttta tcaatctgc 
      taaaagtat ttttcacta tatctagag acttagtaa 
      aaaatactt taataaata atgattaac tacaataat 
      tcttatcac gtacatgct gtagcattt tcaatctag 
      tcaactttg gcatgtact tagataatg cgataaatg 
      caccaaaat caagtgtca ttctaattc atacaattt 
      cggtgtaaa cttgacatt tcttcacta aaaaatcac 
      ttaaattgt tgctctaaa tcaaaaata tcacgtttt 
      tttttaatt gagtagaca tttagctaa tatttagcc 
      ttttactag tatgttctt tgcgcgtgt tggctttat 
      gtgtaataa gctttaagc gaaaatgta gataacatc 
      acgttttac agtaatgtt tataattaa acactactt 
      gcattgtac attgtacct attatattt tgtatgtaa 
      taattacat tacattcac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY