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Gene information
General information
TC007009 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12052137..12067075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007009
    iBeetle screen iB_01107

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007009 RA
      MYQNACGAA AASAGGCVG TERDCHSLI SKEPPVPAI 
EHDYSGFDI VKATQYGAF GRVKELVEA GYNVNQRDS 
ETVTLLHWA AINNRKDII RYFIEKGAE VDAVGGELN 
ATPLHWATR QGHLDAVVI LMNAGTDPT LRDAEGCSC 
IHLAAQFGH TALVAYFIA RGISPDLVD RAGMTPLMW 
SCWKVCSLD PTRLLLTLG ASPNLTDQQ GNTALHWAI 
LARNTSAIS TLIVQGQAS LNVPNHRGD TPLSMLQTQ 
LGAIWIGSK VAERIKEAI SSGHTRNIF TKITKDKRL 
RWWCMVGTP FMAFYIAGS ILGTNLIIL IKLFLLVCL 
YIVLHYAGQ ILYDDRLMA LLPLSIYLS TKLWMYFTW 
FIYIMPATN IITTIVFLS SSGILWYCF LKSWLGDAG 
VISTSQQLR FRTIIELAE QGSGGFEPS TFCSSCLVR 
RPLRSKHCS VCNKCVARF DHHCPWVAN CIGAKNHKY 
FIGFLASLV MMCCQMLYG SVKFWQNQT TCNVTTSEG 
YWKFVLSIA QCDTWVAWV AANTLFHCV WVFTLFVCQ 
LYQISCLGM TTNERMNRG RYSYIVSNG GKSPFSKGP 
IKNILEFFD CTCFGVFKP ENKDWLTSY DLDKNIEHQ 
PLLKNKENF QYV
    CDS sequence
    • >TC007009 RA
      atgtaccaa aacgcttgc ggggcagct gctgcgagt 
gccggcggt tgtgttggc accgaacgt gattgccac 
agtttaata tccaaagaa ccaccagtc ccagcaatc 
gagcacgac tacagcggt ttcgacatc gtcaaagcc 
actcaatat ggtgccttt ggccgcgtc aaagaacta 
gtcgaagcc ggctataac gtcaaccag cgagacagc 
gaaacagtg actttgctc cactgggcg gctatcaac 
aaccgcaaa gacattatt cgttatttt atcgagaag 
ggagctgaa gtggacgct gtggggggc gagttaaat 
gcaaccccc cttcactgg gccacccgc caaggccac 
ctcgatgcc gtggtgatt ttaatgaac gctggcaca 
gatccgacg ctcagagac gctgagggt tgttcttgc 
atacattta gcagcgcag tttggacac acggccctt 
gtggcgtat tttatcgca agggggatt agtcccgat 
ttggtggat agggccggc atgacgcct ttgatgtgg 
tcgtgttgg aaagtttgt agtttggat ccgactaga 
ttgttgttg acgttaggg gcatccccg aatttgaca 
gatcagcaa gggaataca gcactacat tgggcgatt 
ttggctagg aatacgtcg gctatttca acgttgatt 
gttcaggga caagcaagt ctaaatgta cctaatcat 
aggggagat acaccactg tcgatgtta caaacacag 
ttaggggct atttggata ggttccaaa gttgcggaa 
cggattaaa gaagcgata agtagtggg catacaaga 
aatattttt acgaaaatt actaaagat aagagatta 
cgctggtgg tgcatggta ggcaccccc tttatggca 
ttttacatc gctggttcg attttaggc acaaattta 
ataatttta atcaagcta tttctatta gtgtgctta 
tacatagtt ttgcattat gccggtcaa attttatac 
gacgataga ttaatggca cttctacct cttagtatt 
tatttatca acaaaatta tggatgtat ttcacatgg 
tttatttat ataatgcca gccacaaac attataaca 
acaatcgtc ttcctatct agttcagga atattatgg 
tattgcttt ctcaagtca tggttaggt gatgctggt 
gttatttca acttcacaa cagctgaga tttaggact 
ataattgaa ttggccgaa caaggttca ggtggtttc 
gaaccgtcg actttttgt tcgtcttgt ttagtgaga 
agaccgtta agatctaag cattgctca gtgtgcaat 
aaatgtgtc gcaaggttt gatcatcac tgtccgtgg 
gttgctaat tgtataggt gctaaaaat cacaaatat 
ttcatcggt tttttggcc agtttagta atgatgtgt 
tgtcaaatg ttgtacgga tctgtgaaa ttttggcaa 
aaccaaact acttgtaac gtcacaaca tctgaaggt 
tattggaaa tttgtttta tcaattgcg cagtgtgac 
acgtgggtt gcgtgggtt gctgccaat actttattc 
cattgtgtt tgggtcttc acattgttt gtttgtcaa 
ctttatcag atttcgtgt cttggtatg acaacgaac 
gaaagaatg aacagaggg cgctacagt tacattgta 
agtaatggc gggaaaagt ccattttca aaaggtccg 
ataaaaaat attcttgaa tttttcgat tgcacttgt 
ttcggtgtt tttaaaccg gaaaataaa gactggttg 
acgagttac gatttagat aagaatatc gaacatcaa 
ccacttttg aaaaacaaa gagaatttt caatatgtt 
taa
    mRNA sequence
    • >TC007009 RA
      atacttcta atatggggt ttagacgga acagcctgt 
ataagtttt gacatgacc agagtcaca gcagttgtt 
atgacttga cactgacag caatgtgaa atgtcaagt 
ggagcgttt gcagatttg accgccaga gcgcgctgc 
aattttaat accacgatt taactaggt tttattata 
tatcatgtt ttggtaatc actcggcgc atctatatt 
tcatactgt tgttgttgg gtacttaag aatatgttg 
caaacttgt cttttttta atatgttta atatgttga 
aaggtatta tacgtattt agttggagt ttagtgact 
gagacgctc aaattttta ttttagtaa catttttat 
gttttaaaa gtgactttg aaatattat aaatatgta 
atgccaatg aaatttgac ttagaaaac cactccatt 
ccttgttct aacagattt agaaaagaa tagaaaagt 
tagtgctaa ttaggtaag tttaatttt taatttgta 
gtttttaac actatcagc agtgaaagt aagtgaact 
gtttttgtt ttggtttct agttttttt caattttag 
tagtaataa tcaggtatg ttcacagtc tctttcaag 
tagataaga gaagttatt tctagataa ctaacaagt 
tttaagaac ttgttggtt tcttagact caatattga 
tatatgaaa aaagttata aataataaa aaagagtat 
attgagata tccattaaa tgacgtttc aagggtata 
atttccctt ttcttcaga ttaaacaag tttaataca 
aagactttg acacacttg agtgtatct ttctagttt 
taacttctt ttttaatat tggatagtt tcgagtttc 
ttcaatgtt attattgta cgtattttc atattttct 
gtttgattt ttacatttg caaatattt ttctctcac 
taagggccg cttttacaa tcatccgat aaactccgg 
ataagttac ctgtgttag aaatttata ttaggaata 
acaggccga catccgtca gataatttt tttgtcttt 
tacaataca agcaattta tccatggaa taaatgtga 
catttgagc attattgtg acagttagt ttttaacta 
aataatgca agaagttaa cgtatttga atcagacag 
cgatggcaa attaattga gtatgtgga caggttaag 
agtacctac ttaaacaat gttatccaa aaaaatgtc 
cataaagtt ctcaaaaag caaacaacc aatgagaac 
agagtataa atttatcct atagtttat ccggtcgtc 
gaacgacca gataattta ttacgtagt ttataccgt 
caattatcc tatcagggg atcatcgga cgattggaa 
aactgtttt tttaattta ttctaggga taaagttat 
cgagagttt atctggcga tcgtaaaag cggccctaa 
gttgttttt ctgtttgtc ttgctttac ttccttttt 
tttctcatg tgttttttt aacttaaag gaaattatt 
gcagagagg taaagtttt ttgaccctg taaccgcta 
ttactttct ctcttttag aacataaac ataactgaa 
taaagttat agctaatga agttgtcaa caaaaagtt 
attataact tgtgctata atttaaaaa ttgtttgtt 
ggaattggt gttacaaaa ataatcaca aaattcatt 
tcgtagtga ttattgtgt tactgatta tacaggatc 
attcatttc aacgccaac gtcgcattt taccgaatt 
attttttta aataagatt gattaaatt ttcaaatag 
taatgaaaa aactaatta tatttactc tgaccgttt 
cacccatct taattgtac tcaaatatt gctaaaatg 
tgtaaatga tcattctaa ttaacggag agctattcg 
tctcgtcac gaattatcg atttccgcc agtccgaaa 
cctatattg ttcctatat tgaaaccta acctccatt 
tgcattaat gtttgtgtc attctcctg tcaaactta 
atgatcaaa aatttaaaa agatctatt aaaaatgta 
ccaaaacgc ttgcggggc agctgctgc gagtgccgg 
cggttgtgt tggcaccga acgtgattg ccacagttt 
aatatccaa agaaccacc agtcccagc aatcgagca 
cgactacag cggtttcga catcgtcaa agccactca 
atatggtgc ctttggccg cgtcaaaga actagtcga 
agccggcta taacgtcaa ccagcgaga cagcgaaac 
agtgacttt gctccactg ggcggctat caacaaccg 
caaagacat tattcgtta ttttatcga gaagggagc 
tgaagtgga cgctgtggg gggcgagtt aaatgcaac 
cccccttca ctgggccac ccgccaagg ccacctcga 
tgccgtggt gattttaat gaacgctgg cacagatcc 
gacgctcag agacgctga gggttgttc ttgcataca 
tttagcagc gcagtttgg acacacggc ccttgtggc 
gtattttat cgcaagggg gattagtcc cgatttggt 
ggatagggc cggcatgac gcctttgat gtggtcgtg 
ttggaaagt ttgtagttt ggatccgac tagattgtt 
gttgacgtt aggggcatc cccgaattt gacagatca 
gcaagggaa tacagcact acattgggc gattttggc 
taggaatac gtcggctat ttcaacgtt gattgttca 
gggacaagc aagtctaaa tgtacctaa tcatagggg 
agatacacc actgtcgat gttacaaac acagttagg 
ggctatttg gataggttc caaagttgc ggaacggat 
taaagaagc gataagtag tgggcatac aagaaatat 
ttttacgaa aattactaa agataagag attacgctg 
gtggtgcat ggtaggcac cccctttat ggcatttta 
catcgctgg ttcgatttt aggcacaaa tttaataat 
tttaatcaa gctatttct attagtgtg cttatacat 
agttttgca ttatgccgg tcaaatttt atacgacga 
tagattaat ggcacttct acctcttag tatttattt 
atcaacaaa attatggat gtatttcac atggtttat 
ttatataat gccagccac aaacattat aacaacaat 
cgtcttcct atctagttc aggaatatt atggtattg 
ctttctcaa gtcatggtt aggtgatgc tggtgttat 
ttcaacttc acaacagct gagatttag gactataat 
tgaattggc cgaacaagg ttcaggtgg tttcgaacc 
gtcgacttt ttgttcgtc ttgtttagt gagaagacc 
gttaagatc taagcattg ctcagtgtg caataaatg 
tgtcgcaag gtttgatca tcactgtcc gtgggttgc 
taattgtat aggtgctaa aaatcacaa atatttcat 
cggtttttt ggccagttt agtaatgat gtgttgtca 
aatgttgta cggatctgt gaaattttg gcaaaacca 
aactacttg taacgtcac aacatctga aggttattg 
gaaatttgt tttatcaat tgcgcagtg tgacacgtg 
ggttgcgtg ggttgctgc caatacttt attccattg 
tgtttgggt cttcacatt gtttgtttg tcaacttta 
tcagatttc gtgtcttgg tatgacaac gaacgaaag 
aatgaacag agggcgcta cagttacat tgtaagtaa 
tggcgggaa aagtccatt ttcaaaagg tccgataaa 
aaatattct tgaattttt cgattgcac ttgtttcgg 
tgtttttaa accggaaaa taaagactg gttgacgag 
ttacgattt agataagaa tatcgaaca tcaaccact 
tttgaaaaa caaagagaa ttttcaata tgtttaaat 
attgattta aaataataa tccaaagct atgactgag 
acattgaat gattgtcaa tttgtaaag ttagctctc 
taagcagaa acattcctt tttattttg cattgcttc 
cgtaaagtg tcatgtaaa tattttaat tgtaaatac 
gtagtttca ataggattc atttaattg atgcaaaaa 
gagatcaat atttttgcc tcaatttta tcaatctgc 
taaaagtat ttttcacta tatctagag acttagtaa 
aaaatactt taataaata atgattaac tacaataat 
tcttatcac gtacatgct gtagcattt tcaatctag 
tcaactttg gcatgtact tagataatg cgataaatg 
caccaaaat caagtgtca ttctaattc atacaattt 
cggtgtaaa cttgacatt tcttcacta aaaaatcac 
ttaaattgt tgctctaaa tcaaaaata tcacgtttt 
tttttaatt gagtagaca tttagctaa tatttagcc 
ttttactag tatgttctt tgcgcgtgt tggctttat 
gtgtaataa gctttaagc gaaaatgta gataacatc 
acgttttac agtaatgtt tataattaa acactactt 
gcattgtac attgtacct attatattt tgtatgtaa 
taattacat tacattcac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY