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Gene information
General information
TC007006 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12117772..12124190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007006
    iBeetle screen iB_01106

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
    ovary size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Pupal morphology
    wings size decreased - Number of animals showing the phenotype: 2

    legs orientation irregular - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007006 RA
      MALHITKQL LTICKPQTR LLSGLPKLK QTEAPCVIS 
      SRRPRLNHY LNMIYQNFD EIPLVSKGW NHSKSKGDS 
      FTVKPVSTP DAQTIPLNS LNIDSKLIE ALKKRNIET 
      ATDFQANAF SLFDKNKHL LLAAETGSG KTIAYLLPI 
      ICNLITNKT PKLNTPQAL ILVPNRELA YQVGEVAEA 
      LAESLLNVK IIVGGRTKK IMMNPEFGE VDILIGTPG 
      ALGKLCTVG VYKLEEVQF CVLDEADTL IDDSFVDRI 
      VSIIRRMPL AKTILVSAT MPKTLPEAL KPIESNMEQ 
      LVSPKLHKP LLNITQKFM RITRSQKPS HLLQIAKSN 
      KRPMMIFTN RNETCNWLA MFLRENGVS CANINGDMN 
      YAIRIEQWN RFIRGETMI LSATDVGSR GLDTTQVEH 
      VLNYDFPSY AADYLHRIG RIGRLGSSK MCKATNFIS 
      GPEEVRLVQ QIELAIRKN QPLQNVDGN ITNIVQKKI 
      QKGMRQAM
    CDS sequence
    • >TC007006 RA
      atggctctg cacattacc aaacaactt ctcacaata 
      tgtaagccc caaacgaga ctcttgtcg ggcttaccg 
      aagttgaaa caaacagaa gccccatgt gtcataagc 
      tccagacgt ccaagactc aaccactat ttgaacatg 
      atttaccag aacttcgac gaaatccct ttggtttca 
      aaaggctgg aaccactcg aaatctaaa ggcgattcc 
      ttcactgtt aaacctgta agtacccct gacgcgcaa 
      accatccct ttgaattca ctaaacatt gattcaaaa 
      ctaattgag gcgctaaaa aaacgaaac attgaaaca 
      gccactgat tttcaagcc aacgctttc tccttattc 
      gacaagaac aaacatttg ctgcttgca gccgagaca 
      gggtctggt aaaactatt gcatattta ctaccaata 
      atttgtaat ttaattacg aataaaacc ccaaaattg 
      aacacacct caagctttg attcttgtc cctaatcga 
      gaattggcg tatcaggta ggcgaagtg gccgaggca 
      cttgctgag tcactcctc aatgtcaaa ataatagta 
      gggggaagg actaaaaaa atcatgatg aaccccgaa 
      tttggggaa gttgatatt ttgattggc acacctggc 
      gcgttaggg aaattgtgc accgtaggg gtgtacaaa 
      ctcgaagag gtacaattt tgtgttttg gacgaagct 
      gatactcta attgatgat agttttgtt gatcgaatt 
      gtgtcaata atacgtcga atgcctcta gccaaaaca 
      atacttgtc tcagccacg atgccgaaa accttaccc 
      gaagcctta aaaccaatc gaatcaaat atggaacag 
      ctagtttcg ccaaaattg cataaaccc ctcctgaac 
      attacgcag aagtttatg cgcattaca cgatcgcaa 
      aaaccgtcg catttatta caaatcgcc aaaagtaac 
      aaacgccca atgatgatt ttcaccaat cgcaacgag 
      acgtgcaat tggttggcg atgtttttg cgcgaaaat 
      ggtgtttcc tgtgcaaat atcaacggg gatatgaat 
      tatgcaatc agaatagaa caatggaat cggtttata 
      agaggcgag actatgatt ttgtctgcg acagatgtt 
      gggtcaaga gggttggat acgacgcaa gttgagcat 
      gtgctaaat tacgatttt ccctcgtat gctgctgat 
      tatttgcat aggattgga cgaattgga aggttgggg 
      agttcaaag atgtgtaaa gcaacgaat tttatttcg 
      gggcctgag gaagttcga ttggtgcaa cagattgag 
      ttggcaatt cggaaaaat caacctctt caaaatgtc 
      gatggaaac ataacaaat attgtacaa aaaaagata 
      caaaaaggg atgcgtcaa gcgatgtaa
    mRNA sequence
    • >TC007006 RA
      tggaaaata atgtgtcac gcgtcagaa atagtaagg 
      ttaggttcg tctaacctc gaaatggct ctgcacatt 
      accaaacaa cttctcaca atatgtaag ccccaaacg 
      agactcttg tcgggctta ccgaagttg aaacaaaca 
      gaagcccca tgtgtcata agctccaga cgtccaaga 
      ctcaaccac tatttgaac atgatttac cagaacttc 
      gacgaaatc cctttggtt tcaaaaggc tggaaccac 
      tcgaaatct aaaggcgat tccttcact gttaaacct 
      gtaagtacc cctgacgcg caaaccatc cctttgaat 
      tcactaaac attgattca aaactaatt gaggcgcta 
      aaaaaacga aacattgaa acagccact gattttcaa 
      gccaacgct ttctcctta ttcgacaag aacaaacat 
      ttgctgctt gcagccgag acagggtct ggtaaaact 
      attgcatat ttactacca ataatttgt aatttaatt 
      acgaataaa accccaaaa ttgaacaca cctcaagct 
      ttgattctt gtccctaat cgagaattg gcgtatcag 
      gtaggcgaa gtggccgag gcacttgct gagtcactc 
      ctcaatgtc aaaataata gtaggggga aggactaaa 
      aaaatcatg atgaacccc gaatttggg gaagttgat 
      attttgatt ggcacacct ggcgcgtta gggaaattg 
      tgcaccgta ggggtgtac aaactcgaa gaggtacaa 
      ttttgtgtt ttggacgaa gctgatact ctaattgat 
      gatagtttt gttgatcga attgtgtca ataatacgt 
      cgaatgcct ctagccaaa acaatactt gtctcagcc 
      acgatgccg aaaacctta cccgaagcc ttaaaacca 
      atcgaatca aatatggaa cagctagtt tcgccaaaa 
      ttgcataaa cccctcctg aacattacg cagaagttt 
      atgcgcatt acacgatcg caaaaaccg tcgcattta 
      ttacaaatc gccaaaagt aacaaacgc ccaatgatg 
      attttcacc aatcgcaac gagacgtgc aattggttg 
      gcgatgttt ttgcgcgaa aatggtgtt tcctgtgca 
      aatatcaac ggggatatg aattatgca atcagaata 
      gaacaatgg aatcggttt ataagaggc gagactatg 
      attttgtct gcgacagat gttgggtca agagggttg 
      gatacgacg caagttgag catgtgcta aattacgat 
      tttccctcg tatgctgct gattatttg cataggatt 
      ggacgaatt ggaaggttg gggagttca aagatgtgt 
      aaagcaacg aattttatt tcggggcct gaggaagtt 
      cgattggtg caacagatt gagttggca attcggaaa 
      aatcaacct cttcaaaat gtcgatgga aacataaca 
      aatattgta caaaaaaag atacaaaaa gggatgcgt 
      caagcgatg taaaaactg ttttattct aaatataag 
      ttaccaaag atcgtgttt atttattgt cctcactgt 
      cgctatcac tcgatgata actcttcgt ctatttcaa 
      gtctgtttc tattcacta acaaatcac tctcatcct 
      cttggtcct ttttattgt cagaaaatt caggtggcc 
      atacgttgt cttctttca aacatttga tgtgttcat 
      tcgttaata ttcgcacta tttcagctt tgactttat 
      cgccttcgg tgattggtt caacaagga cgtaactgc 
      cacgtttca cccatatat ttttacgga attttgttg 
      gcatggaca ccaaataag tggattggt ttggtgcca 
      gaacttcgt gtaagttgt tgcctttgg tgctaatta 
      ttcggacga tggtttggt tttcagttg gtaaagaca 
      tgtcatcgt ctagtgctt ctttgaaaa cgtgtttcc 
      gcttggtct tgcgggaca ttctgcaat gtaaagctt 
      tctatcaac aacaaaata aattataca ttcataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY