General information | |||
TC007006 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 12117772..12124190 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007006 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01106 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)ovary size decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC007006 RA
MALHITKQL LTICKPQTR LLSGLPKLK QTEAPCVIS SRRPRLNHY LNMIYQNFD EIPLVSKGW NHSKSKGDS FTVKPVSTP DAQTIPLNS LNIDSKLIE ALKKRNIET ATDFQANAF SLFDKNKHL LLAAETGSG KTIAYLLPI ICNLITNKT PKLNTPQAL ILVPNRELA YQVGEVAEA LAESLLNVK IIVGGRTKK IMMNPEFGE VDILIGTPG ALGKLCTVG VYKLEEVQF CVLDEADTL IDDSFVDRI VSIIRRMPL AKTILVSAT MPKTLPEAL KPIESNMEQ LVSPKLHKP LLNITQKFM RITRSQKPS HLLQIAKSN KRPMMIFTN RNETCNWLA MFLRENGVS CANINGDMN YAIRIEQWN RFIRGETMI LSATDVGSR GLDTTQVEH VLNYDFPSY AADYLHRIG RIGRLGSSK MCKATNFIS GPEEVRLVQ QIELAIRKN QPLQNVDGN ITNIVQKKI QKGMRQAM
- >TC007006 RA
atggctctg cacattacc aaacaactt ctcacaata tgtaagccc caaacgaga ctcttgtcg ggcttaccg aagttgaaa caaacagaa gccccatgt gtcataagc tccagacgt ccaagactc aaccactat ttgaacatg atttaccag aacttcgac gaaatccct ttggtttca aaaggctgg aaccactcg aaatctaaa ggcgattcc ttcactgtt aaacctgta agtacccct gacgcgcaa accatccct ttgaattca ctaaacatt gattcaaaa ctaattgag gcgctaaaa aaacgaaac attgaaaca gccactgat tttcaagcc aacgctttc tccttattc gacaagaac aaacatttg ctgcttgca gccgagaca gggtctggt aaaactatt gcatattta ctaccaata atttgtaat ttaattacg aataaaacc ccaaaattg aacacacct caagctttg attcttgtc cctaatcga gaattggcg tatcaggta ggcgaagtg gccgaggca cttgctgag tcactcctc aatgtcaaa ataatagta gggggaagg actaaaaaa atcatgatg aaccccgaa tttggggaa gttgatatt ttgattggc acacctggc gcgttaggg aaattgtgc accgtaggg gtgtacaaa ctcgaagag gtacaattt tgtgttttg gacgaagct gatactcta attgatgat agttttgtt gatcgaatt gtgtcaata atacgtcga atgcctcta gccaaaaca atacttgtc tcagccacg atgccgaaa accttaccc gaagcctta aaaccaatc gaatcaaat atggaacag ctagtttcg ccaaaattg cataaaccc ctcctgaac attacgcag aagtttatg cgcattaca cgatcgcaa aaaccgtcg catttatta caaatcgcc aaaagtaac aaacgccca atgatgatt ttcaccaat cgcaacgag acgtgcaat tggttggcg atgtttttg cgcgaaaat ggtgtttcc tgtgcaaat atcaacggg gatatgaat tatgcaatc agaatagaa caatggaat cggtttata agaggcgag actatgatt ttgtctgcg acagatgtt gggtcaaga gggttggat acgacgcaa gttgagcat gtgctaaat tacgatttt ccctcgtat gctgctgat tatttgcat aggattgga cgaattgga aggttgggg agttcaaag atgtgtaaa gcaacgaat tttatttcg gggcctgag gaagttcga ttggtgcaa cagattgag ttggcaatt cggaaaaat caacctctt caaaatgtc gatggaaac ataacaaat attgtacaa aaaaagata caaaaaggg atgcgtcaa gcgatgtaa
- >TC007006 RA
tggaaaata atgtgtcac gcgtcagaa atagtaagg ttaggttcg tctaacctc gaaatggct ctgcacatt accaaacaa cttctcaca atatgtaag ccccaaacg agactcttg tcgggctta ccgaagttg aaacaaaca gaagcccca tgtgtcata agctccaga cgtccaaga ctcaaccac tatttgaac atgatttac cagaacttc gacgaaatc cctttggtt tcaaaaggc tggaaccac tcgaaatct aaaggcgat tccttcact gttaaacct gtaagtacc cctgacgcg caaaccatc cctttgaat tcactaaac attgattca aaactaatt gaggcgcta aaaaaacga aacattgaa acagccact gattttcaa gccaacgct ttctcctta ttcgacaag aacaaacat ttgctgctt gcagccgag acagggtct ggtaaaact attgcatat ttactacca ataatttgt aatttaatt acgaataaa accccaaaa ttgaacaca cctcaagct ttgattctt gtccctaat cgagaattg gcgtatcag gtaggcgaa gtggccgag gcacttgct gagtcactc ctcaatgtc aaaataata gtaggggga aggactaaa aaaatcatg atgaacccc gaatttggg gaagttgat attttgatt ggcacacct ggcgcgtta gggaaattg tgcaccgta ggggtgtac aaactcgaa gaggtacaa ttttgtgtt ttggacgaa gctgatact ctaattgat gatagtttt gttgatcga attgtgtca ataatacgt cgaatgcct ctagccaaa acaatactt gtctcagcc acgatgccg aaaacctta cccgaagcc ttaaaacca atcgaatca aatatggaa cagctagtt tcgccaaaa ttgcataaa cccctcctg aacattacg cagaagttt atgcgcatt acacgatcg caaaaaccg tcgcattta ttacaaatc gccaaaagt aacaaacgc ccaatgatg attttcacc aatcgcaac gagacgtgc aattggttg gcgatgttt ttgcgcgaa aatggtgtt tcctgtgca aatatcaac ggggatatg aattatgca atcagaata gaacaatgg aatcggttt ataagaggc gagactatg attttgtct gcgacagat gttgggtca agagggttg gatacgacg caagttgag catgtgcta aattacgat tttccctcg tatgctgct gattatttg cataggatt ggacgaatt ggaaggttg gggagttca aagatgtgt aaagcaacg aattttatt tcggggcct gaggaagtt cgattggtg caacagatt gagttggca attcggaaa aatcaacct cttcaaaat gtcgatgga aacataaca aatattgta caaaaaaag atacaaaaa gggatgcgt caagcgatg taaaaactg ttttattct aaatataag ttaccaaag atcgtgttt atttattgt cctcactgt cgctatcac tcgatgata actcttcgt ctatttcaa gtctgtttc tattcacta acaaatcac tctcatcct cttggtcct ttttattgt cagaaaatt caggtggcc atacgttgt cttctttca aacatttga tgtgttcat tcgttaata ttcgcacta tttcagctt tgactttat cgccttcgg tgattggtt caacaagga cgtaactgc cacgtttca cccatatat ttttacgga attttgttg gcatggaca ccaaataag tggattggt ttggtgcca gaacttcgt gtaagttgt tgcctttgg tgctaatta ttcggacga tggtttggt tttcagttg gtaaagaca tgtcatcgt ctagtgctt ctttgaaaa cgtgtttcc gcttggtct tgcgggaca ttctgcaat gtaaagctt tctatcaac aacaaaata aattataca ttcataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
