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Gene information
General information
TC006985 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid908: 44..9207
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006985
    iBeetle screen iB_01104
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006985 RA
      MLLPAQISS TILILMLLH VNAEAPPGY IKNEDSAAK 
      NPPKYEYGY NIQDEKGSS HGKLETRDG IYALGRYYV 
      QNSDSSQNV QYYADDWGY HPFIEYSNV GPHSRTKTQ 
      LLLGVEAVK ALASNKFTP NSLPAKQHV TDIINSKIN 
      NRDGKQAGQ PQLVVAQQN HQTLVEVQQ PQEVVNEQP 
      QQIVVEHQP QNQQIIVER PQHILLDEQ QQQQQQISE 
      QQQQQQISE QQQQQQISE QPQQTPVDD SITVEAISN 
      RQAQLQEQP QLNHVLTQQ EISFQQSNF NQPKSEPQI 
      EEIQIREGI SHQQIPVPQ AQSLPKEND KQIQDNLVF 
      GGNYQQSHS NLGGFGSQH NLLGRRPPN NINRQLQQT 
      SGRLIETTK NLVSGQDVL NINSAIDQS VETSVESST 
      VNSPFESAS LLQEPIVVA DDLEQVKEN VFSTTSSTI 
      ETISTKSFG VTEPSTLLV TPRPVSTNF LAPITAGIQ 
      LTNIKNTDC NQDSESSQK IESTARKEN YVVEIQKSL 
      PYYLGKYEY GSSAVEGDT QSQSGNLEG IAVENIELG 
      KTLLALPGQ PTLNQNNLQ QQQQLPQVS IKENFVDQQ 
      QAQGNHQVK VVTVPVIQT KIVEKPVQV TKYIQTPYP 
      VPVEIRVPI EKHIPVTVE KIVEKKVHV PQPYPVEKI 
      VEKHVPVEV TRFIDRPYP VQVPVATPV PYPVDRVVE 
      KIVKQPYPV EVRVPVPVE KIVEKKVSV PVDRIVEKP 
      VPQFIDRPV QVQVPVPVA QIYTLQLSK ALPPILKPP 
      QQTQSSQKI SVTLRNLNN YRQQNQPLF VQVPNNNNN 
      GYLPPHNCD QHSSYSSNT NSYYTIQAD DYIGLLPPR 
      LQDDEGPTK FRNPRSNFN SNLRLEYGF MPPLRPSQE 
      IDEHGNPIT REKP
    CDS sequence
    • >TC006985 RA
      atgctgctg ccggcacag atttcttct acaattttg 
      atattaatg ttactgcat gtcaatgct gaggcacct 
      cctggctat ataaagaat gaagattca gcagcaaaa 
      aatcctcca aaatacgaa tatggatac aacattcaa 
      gatgagaaa ggctcatct catggtaaa ctagaaact 
      agagatgga atttatgca cttggaaga tactatgta 
      caaaacagt gattcttct caaaacgtt cagtactat 
      gctgatgat tggggttat catcccttt attgaatat 
      agtaatgtg ggaccacac agtcgtaca aaaacacag 
      ttattgcta ggtgtcgaa gctgttaaa gctttagcg 
      tcaaataaa tttactcca aactctcta cctgctaaa 
      caacacgta acagatatt atcaacagc aaaattaac 
      aacagagat ggaaaacaa gcaggacag cctcaatta 
      gttgttgca caacaaaac catcagaca ttagttgaa 
      gtacaacag ccccaagaa gttgtgaac gagcaaccc 
      caacaaatt gtcgtagaa catcaacct caaaatcag 
      caaattata gtcgaacgc ccacaacac attttacta 
      gatgagcaa caacaacaa caacaacag atatcagaa 
      caacaacaa caacaacag atatcagaa caacaacaa 
      caacaacag atatcagaa caaccgcaa caaacacct 
      gtagacgac tcgattaca gttgaagca ataagtaat 
      cggcaagca caacttcaa gaacaacct cagttgaat 
      cacgtacta acacaacaa gaaatttcc tttcaacag 
      tccaatttt aatcaaccg aaatctgag ccccaaatt 
      gaagaaata caaattcga gaaggtatt tcacatcag 
      caaataccc gtacctcaa gcacaaagt cttccaaaa 
      gaaaatgat aaacaaatt caagataat ttagtattt 
      ggtggaaat tatcagcag tctcattca aacttgggt 
      ggttttggt tctcagcat aatctttta ggccgaaga 
      ccaccaaat aacattaac agacagttg cagcaaact 
      tcaggcaga cttattgaa actacaaaa aatctcgtg 
      tcgggacaa gatgtttta aatattaac agtgcaatc 
      gatcaaagc gtggaaaca agtgttgaa agtagtact 
      gttaattca cctttcgag agtgcaagt ttactgcaa 
      gaaccgatt gtagttgca gatgattta gaacaagtc 
      aaagaaaat gtattttca acaacatcc agtactatt 
      gaaacaata tcgacaaaa tcttttggt gtaacagaa 
      ccgtcaaca cttttggtc actcctcga ccagttagt 
      accaatttt ttggcgccc atcacagca ggaattcaa 
      cttacaaac attaaaaac acggattgt aaccaagat 
      tccgaaagc agccagaaa attgaatcc actgctcga 
      aaagaaaat tatgttgtt gaaattcaa aaaagcctt 
      ccatattat ttaggtaaa tacgaatat ggatcaagt 
      gctgttgaa ggagacaca caaagtcag tctggaaat 
      ttagaaggg atagcagtt gaaaacatc gaattgggt 
      aaaacgctt ctagcattg cctggacag ccaacttta 
      aatcagaat aatttgcaa caacaacaa caacttccg 
      caagtgtcg ataaaagaa aactttgtc gatcagcaa 
      caagcgcaa ggcaatcat caggtgaaa gttgtaacg 
      gtaccagtt attcaaacg aaaattgtc gagaaaccc 
      gttcaagtt acaaagtac attcaaaca ccatatccg 
      gttcctgtg gaaataaga gtaccaatc gaaaagcat 
      attcctgtg accgttgaa aaaattgtc gaaaaaaaa 
      gttcacgtg cctcaaccg tatccagtt gaaaaaatt 
      gtggaaaaa catgtacct gttgaagta acaagattt 
      attgacaga ccatatcct gtacaagtt ccagtagca 
      acacctgta ccatatcca gttgatcgg gtcgttgaa 
      aaaattgta aaacaacca tatccagtg gaagtgcgt 
      gttccagtt cccgtagaa aaaattgtc gaaaaaaaa 
      gtttcagtt ccagttgat cgtatagtt gagaaacct 
      gtaccacaa tttatagac agacccgtg caagtgcaa 
      gttccagta cccgttgcc caaatttac actttacaa 
      ctatcaaaa gctttgccc ccaatttta aaaccacca 
      caacagaca caatcgtct cagaaaatt tcagtgact 
      cttagaaat ctgaacaat tatcgacaa caaaatcag 
      cctttattc gtgcaagta ccaaacaac aataataac 
      ggttatttg ccaccacac aactgcgat caacacagc 
      agttacagc agcaataca aattcgtat tacaccata 
      caagcagat gactatatt ggtcttttg ccaccaaga 
      ttacaagac gatgagggt cccacaaaa tttagaaat 
      ccacgctcg aattttaac tcaaatcta agattggaa 
      tacggtttc atgccacct ctaaggcct tcgcaagaa 
      atcgacgaa cacggaaac ccgattacc agggagaaa 
      ccataa
    mRNA sequence
    • >TC006985 RA
      gacctgaca gacctgact tgactgacc tgacctgac 
      ctgacctga cctgacatt acctgacat tacctgaca 
      ttacctgac ctgacctga cctgacctg acctgacct 
      gacctgacc tgacctgac attacctga cattacctg 
      acatgacct gacatgacc tgacatgac cgacctcga 
      gtcggatgg ctaacgcac tgtaaaaaa aaaagaatt 
      aattaatta atttaagtg ttaatttat ggggcaggt 
      gaaaggact tgtgtaagg aatcagatg aaattctga 
      tacatttct aaaagagat cagtcgtga agagcatac 
      tacacatct ctctccagc tgctaaagt cttggtttc 
      caacagtat catagttag tgatagttc tttattggg 
      tgaggtgaa ctttcaaga aatgctgct gccggcaca 
      gatttcttc tacaatttt gatattaat gttactgca 
      tgtcaatgc tgaggcacc tcctggcta tataaagaa 
      tgaagattc agcagcaaa aaatcctcc aaaatacga 
      atatggata caacattca agatgagaa aggctcatc 
      tcatggtaa actagaaac tagagatgg aatttatgc 
      acttggaag atactatgt acaaaacag tgattcttc 
      tcaaaacgt tcagtacta tgctgatga ttggggtta 
      tcatccctt tattgaata tagtaatgt gggaccaca 
      cagtcgtac aaaaacaca gttattgct aggtgtcga 
      agctgttaa agctttagc gtcaaataa atttactcc 
      aaactctct acctgctaa acaacacgt aacagatat 
      tatcaacag caaaattaa caacagaga tggaaaaca 
      agcaggaca gcctcaatt agttgttgc acaacaaaa 
      ccatcagac attagttga agtacaaca gccccaaga 
      agttgtgaa cgagcaacc ccaacaaat tgtcgtaga 
      acatcaacc tcaaaatca gcaaattat agtcgaacg 
      cccacaaca cattttact agatgagca acaacaaca 
      acaacaaca gatatcaga acaacaaca acaacaaca 
      gatatcaga acaacaaca acaacaaca gatatcaga 
      acaaccgca acaaacacc tgtagacga ctcgattac 
      agttgaagc aataagtaa tcggcaagc acaacttca 
      agaacaacc tcagttgaa tcacgtact aacacaaca 
      agaaatttc ctttcaaca gtccaattt taatcaacc 
      gaaatctga gccccaaat tgaagaaat acaaattcg 
      agaaggtat ttcacatca gcaaatacc cgtacctca 
      agcacaaag tcttccaaa agaaaatga taaacaaat 
      tcaagataa tttagtatt tggtggaaa ttatcagca 
      gtctcattc aaacttggg tggttttgg ttctcagca 
      taatctttt aggccgaag accaccaaa taacattaa 
      cagacagtt gcagcaaac ttcaggcag acttattga 
      aactacaaa aaatctcgt gtcgggaca agatgtttt 
      aaatattaa cagtgcaat cgatcaaag cgtggaaac 
      aagtgttga aagtagtac tgttaattc acctttcga 
      gagtgcaag tttactgca agaaccgat tgtagttgc 
      agatgattt agaacaagt caaagaaaa tgtattttc 
      aacaacatc cagtactat tgaaacaat atcgacaaa 
      atcttttgg tgtaacaga accgtcaac acttttggt 
      cactcctcg accagttag taccaattt tttggcgcc 
      catcacagc aggaattca acttacaaa cattaaaaa 
      cacggattg taaccaaga ttccgaaag cagccagaa 
      aattgaatc cactgctcg aaaagaaaa ttatgttgt 
      tgaaattca aaaaagcct tccatatta tttaggtaa 
      atacgaata tggatcaag tgctgttga aggagacac 
      acaaagtca gtctggaaa tttagaagg gatagcagt 
      tgaaaacat cgaattggg taaaacgct tctagcatt 
      gcctggaca gccaacttt aaatcagaa taatttgca 
      acaacaaca acaacttcc gcaagtgtc gataaaaga 
      aaactttgt cgatcagca acaagcgca aggcaatca 
      tcaggtgaa agttgtaac ggtaccagt tattcaaac 
      gaaaattgt cgagaaacc cgttcaagt tacaaagta 
      cattcaaac accatatcc ggttcctgt ggaaataag 
      agtaccaat cgaaaagca tattcctgt gaccgttga 
      aaaaattgt cgaaaaaaa agttcacgt gcctcaacc 
      gtatccagt tgaaaaaat tgtggaaaa acatgtacc 
      tgttgaagt aacaagatt tattgacag accatatcc 
      tgtacaagt tccagtagc aacacctgt accatatcc 
      agttgatcg ggtcgttga aaaaattgt aaaacaacc 
      atatccagt ggaagtgcg tgttccagt tcccgtaga 
      aaaaattgt cgaaaaaaa agtttcagt tccagttga 
      tcgtatagt tgagaaacc tgtaccaca atttataga 
      cagacccgt gcaagtgca agttccagt acccgttgc 
      ccaaattta cactttaca actatcaaa agctttgcc 
      cccaatttt aaaaccacc acaacagac acaatcgtc 
      tcagaaaat ttcagtgac tcttagaaa tctgaacaa 
      ttatcgaca acaaaatca gcctttatt cgtgcaagt 
      accaaacaa caataataa cggttattt gccaccaca 
      caactgcga tcaacacag cagttacag cagcaatac 
      aaattcgta ttacaccat acaagcaga tgactatat 
      tggtctttt gccaccaag attacaaga cgatgaggg 
      tcccacaaa atttagaaa tccacgctc gaattttaa 
      ctcaaatct aagattgga atacggttt catgccacc 
      tctaaggcc ttcgcaaga aatcgacga acacggaaa 
      cccgattac cagggagaa accataatg atgagaaaa 
      tattttcat tagtgatat tttcaaatg ccagacata 
      aaggcattt taaaactaa taagtcact tcattttag 
      ggaaggttt tattgcttt tggttacct accgtattt 
      aggatgttc ctcagggtt ctccataag cgctacatt 
      ttgtttaac tataattca agtgtcatg tcatctgcc 
      atttttact gttaagttt cattattat ctattatat 
      ttatttata tcaaatatt taattttaa ttcaactca 
      tgaataatc ttgtgaata cttaggttg tgtatgtaa 
      ataaataaa caaaaataa g