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Gene information
General information
TC034974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 156990..164513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034974
    iBeetle screen iB_01103

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034974 RA
      MNRQYPPHQ QQFGLPPQE MLGGHTSPL PPGPPPPQG 
      FQNVNLGPQ KPPQPYMYP PGQMPTNPR PVPPSQLPP 
      EQEQKPEQF VPPMPKPGG GVQNGPQFQ NGPPNSINK 
      QLPPQQMPR PGPPPQPYT QPPPPSNDH QGPPAPRQY 
      PTLPGQPPS VPIQPNPML AQPPPPNHF NQPPPNRQF 
      NQPPMSNLN QPPMPNLNH PPMSNLNQP PMPNLNQPP 
      MPNLNQPPP MPGPNQLAN QMQGMNLSG PAPGAYPPG 
      PSMKRATNG DGGPHMPPP LTQQQYMNG QQMRGQQGF 
      GQPPVGGAM GAYPQPAYQ QPQQAKRLD PDQMPSPIQ 
      VTQDDQQNR SGIFCTNQK GLVPPLVTT NFIVQDQGN 
      SSPRFIRSS MYNVPISQD IMKQTAVPF SLVISPMAR 
      TVDQEYPPP IVNFGELGP VRCIRCKAY MCPFMQFID 
      SGRRFQCLF CKATTDVPQ EYFQHLDHT GQRLDRFER 
      PELVLGTYE FVATPEYCR NNTLPKPPA IIFVIDVSY 
      NNIKSGLVH LLCAQIKEI IQHLPVDQG QEKSNMRVG 
      FITYNSSVH FYNIKGNLA APQMLIVGD IQEMFMPLL 
      DGFLCTPEE SGPVIDALM QQIPAMFGD TRETETILL 
      PAILAGLEA LKASECAGK LCVFHSSLP TAEGPGKLK 
      NRDDRKLLG TDKERTVLT PQTQAYNQL GQDCVAAGC 
      SVDLFIFNN AYIDIATIS QVSRLTGGE VHKYTYFQA 
      DIDGERLIK DIITNISRP IAFDAIMRV RTSTGVRPT 
      DFYGHFYMS NTTDMELAA IDCDKAVAV EIKHDDKLS 
      EEEGVYIQA ALLYTSCSG QRRLRVMNL SLKTCSQMS 
      DLYRNCDCD TIINYVSKQ ATYKLYEHN PKAVKDSIV 
      TRAAQILAS YRKNCASPS SAGQLILPE CMKLLPLYV 
      NCLLKSDAL SGGSDMTID DRSYVMQEV MIMDIPTSV 
      NYFYPRLIP LIDLDVSQD PVQIPSPIR CTIDKMSEQ 
      GVYILENGI HMFLWIGLE ANPDFIQKV FGVPSAIQV 
      DVEKVALPE LDNSLSVAV RNIIEEIRI QRHKCMRLT 
      IVRQREKLE PVFKHFLAE DRGTDGSPS YVDFLIHMH 
      KEIRSLLS
    CDS sequence
    • >TC034974 RA
      atgaatcgt caataccct ccacatcag caacagttc 
      gggttgcct cctcaagag atgttaggg gggcacact 
      tctccttta cccccaggc cctccacca ccacaaggt 
      tttcagaat gttaattta gggccccaa aaaccacca 
      caaccttac atgtaccct cctggacaa atgcccaca 
      aaccctagg cctgtgccc ccaagccaa ttaccacca 
      gaacaggag cagaagcct gagcagttt gtgccccca 
      atgcctaaa cctggaggt ggtgttcaa aatgggcct 
      caattccaa aatggacct ccaaactca ataaataaa 
      caactccct ccacaacaa atgcccaga cctggaccc 
      cctcctcaa ccttacact caacctcct ccaccctcc 
      aacgaccat caaggtcct ccagcacct agacaatac 
      ccaacactc cctggacaa cctccttca gtacccatt 
      caaccaaac ccaatgtta gctcaacct ccaccacct 
      aatcacttt aaccaaccg ccacccaac cgtcaattt 
      aatcaacct cccatgtct aatttaaat caacctcca 
      atgcctaat ttaaatcat cctccaatg tctaattta 
      aatcaacct ccaatgcct aatttaaat caacctcca 
      atgcccaat ctaaatcaa ccccctcca atgcctgga 
      cccaaccaa ttagccaat caaatgcaa ggcatgaat 
      ttgagtgga cctgctcct ggggcatat cctccaggt 
      ccttcaatg aaaagggca acgaatgga gatgggggc 
      ccccatatg ccaccccct ttaactcaa caacaatac 
      atgaatgga caacaaatg aggggtcaa caagggttc 
      ggacagccc cctgttgga ggagctatg ggggcttat 
      ccacagcct gcttatcaa cagccacaa caagcgaaa 
      cgacttgat ccagatcaa atgccgagt cctattcaa 
      gtgacccaa gacgatcag caaaaccgc tccggtatt 
      ttttgcacc aatcaaaaa ggtttggtg cctccttta 
      gtaacaacc aactttatt gttcaagat caaggcaat 
      tccagtcca cgatttata cgttctagc atgtacaac 
      gtgccaata tcacaggac ataatgaag caaactgca 
      gttcccttc agtttggtc attagtcca atggctcga 
      acagtcgat caagaatac cctccacct attgtcaat 
      tttggggaa ctaggacca gttcgttgt atcagatgc 
      aaggcctac atgtgccct tttatgcaa tttattgat 
      tcgggaagg cgtttccag tgccttttt tgtaaagca 
      acaactgat gtaccccaa gaatatttc caacatttg 
      gatcacact ggtcaaaga ttggaccgt tttgaacgt 
      ccagaactt gttttgggc acttatgag ttcgttgca 
      actcctgaa tattgtcgg aataatact ttacctaaa 
      ccaccagct atcattttc gttattgat gtttcttac 
      aataacatt aaatcaggt ttagtacat ttattatgt 
      gcacaaatt aaggagatt atccagcat ttaccggtg 
      gatcaagga caggaaaaa agcaatatg cgagttggg 
      tttattaca tacaacagc tcagtgcat ttttacaac 
      attaagggg aacttggct gctcctcaa atgctaatt 
      gttggtgac atccaagaa atgtttatg ccccttttg 
      gacggattt ttatgcaca ccggaagaa tccggtcct 
      gttatcgac gcattgatg caacaaatt ccagcaatg 
      tttggtgac acaagagaa acagaaacc atactttta 
      ccggcgatt ttagcagga ctagaggct ttaaaagcg 
      tccgaatgt gcgggaaaa ttatgcgtt ttccattcg 
      agtttgcca acagctgaa ggacctgga aaattgaaa 
      aatcgtgac gatcgtaaa ttactagga actgataaa 
      gaacgaact gttttaaca ccacaaaca caagcctat 
      aatcagtta ggtcaggat tgtgtagct gccggttgt 
      tcagttgat ctttttatt tttaataac gcatacatt 
      gatattgca acaatcagt caagtgtcg aggttgaca 
      gggggcgaa gttcacaaa tatacatat ttccaggcc 
      gatattgat ggtgagaga ttaataaaa gatattata 
      acgaatatc agcagacct atcgcattt gatgctatt 
      atgagagta cggacgtca actggtgtc agacccact 
      gatttttat gggcatttt tacatgtca aataccaca 
      gatatggaa ctagcagcc attgattgt gataaagca 
      gtggctgtt gaaataaaa cacgacgac aaactatct 
      gaagaagaa ggtgtttac atccaggcg gctttattg 
      tatacgtca tgttcgggt caacgccgc ctgcgcgta 
      atgaatctt tcactgaaa acttgctca caaatgtcc 
      gacctttac cgaaactgt gattgtgat acgataatc 
      aattacgtt tcgaaacag gcaacttat aaattatac 
      gaacataat ccgaaagct gtaaaagac agtatagtg 
      acacgagct gcgcaaatt ttagcaagc tatcgcaaa 
      aactgtgct agtccaagt agcgcaggt cagttgatt 
      ttgcccgaa tgtatgaaa ttattgcct ttgtatgtg 
      aattgtcta ttgaagagc gatgctctg tctggaggc 
      tctgatatg acaatagac gatcggtcg tatgttatg 
      caggaggtc atgattatg gatataccc acgagtgtg 
      aattatttc tatccaagg ttgattcct ttgattgat 
      ttggatgtg agtcaagat cctgttcag attccttcg 
      cctattagg tgtacgata gataaaatg agtgagcaa 
      ggtgtttat atcttggaa aatgggata cacatgttt 
      ttgtggatt ggacttgaa gccaatcct gatttcatc 
      cagaaagtt tttggagtc ccgtctgcc attcaagtg 
      gatgtggaa aaagttgca ttgcctgaa ttggataat 
      tcgttgtcg gttgctgta agaaatatt attgaagaa 
      atcaggata cagagacat aaatgcatg aggttaact 
      attgttcgt cagagagag aagttggag cccgttttt 
      aaacatttc ctagctgaa gatcgtgga acggatgga 
      tctccgagt tatgtcgac tttttaata catatgcac 
      aaagaaatt cgttcgttg ttaagctag
    mRNA sequence
    • >TC034974 RA
      cgcatgcgc tgtttaacc ttacgtggc aaagtgcaa 
      gtgagggtt agagaaatg tcgtttatt tattaattt 
      tttcaagtg ttttgttgg acgatttag tgtacgtgt 
      taatttttt acgtcctgg attacaaag tataaaaaa 
      tgaatcgtc aataccctc cacatcagc aacagttcg 
      ggttgcctc ctcaagaga tgttagggg ggcacactt 
      ctcctttac ccccaggcc ctccaccac cacaaggtt 
      ttcagaatg ttaatttag ggccccaaa aaccaccac 
      aaccttaca tgtaccctc ctggacaaa tgcccacaa 
      accctaggc ctgtgcccc caagccaat taccaccag 
      aacaggagc agaagcctg agcagtttg tgcccccaa 
      tgcctaaac ctggaggtg gtgttcaaa atgggcctc 
      aattccaaa atggacctc caaactcaa taaataaac 
      aactccctc cacaacaaa tgcccagac ctggacccc 
      ctcctcaac cttacactc aacctcctc caccctcca 
      acgaccatc aaggtcctc cagcaccta gacaatacc 
      caacactcc ctggacaac ctccttcag tacccattc 
      aaccaaacc caatgttag ctcaacctc caccaccta 
      atcacttta accaaccgc cacccaacc gtcaattta 
      atcaacctc ccatgtcta atttaaatc aacctccaa 
      tgcctaatt taaatcatc ctccaatgt ctaatttaa 
      atcaacctc caatgccta atttaaatc aacctccaa 
      tgcccaatc taaatcaac cccctccaa tgcctggac 
      ccaaccaat tagccaatc aaatgcaag gcatgaatt 
      tgagtggac ctgctcctg gggcatatc ctccaggtc 
      cttcaatga aaagggcaa cgaatggag atgggggcc 
      cccatatgc caccccctt taactcaac aacaataca 
      tgaatggac aacaaatga ggggtcaac aagggttcg 
      gacagcccc ctgttggag gagctatgg gggcttatc 
      cacagcctg cttatcaac agccacaac aagcgaaac 
      gacttgatc cagatcaaa tgccgagtc ctattcaag 
      tgacccaag acgatcagc aaaaccgct ccggtattt 
      tttgcacca atcaaaaag gtttggtgc ctcctttag 
      taacaacca actttattg ttcaagatc aaggcaatt 
      ccagtccac gatttatac gttctagca tgtacaacg 
      tgccaatat cacaggaca taatgaagc aaactgcag 
      ttcccttca gtttggtca ttagtccaa tggctcgaa 
      cagtcgatc aagaatacc ctccaccta ttgtcaatt 
      ttggggaac taggaccag ttcgttgta tcagatgca 
      aggcctaca tgtgccctt ttatgcaat ttattgatt 
      cgggaaggc gtttccagt gcctttttt gtaaagcaa 
      caactgatg taccccaag aatatttcc aacatttgg 
      atcacactg gtcaaagat tggaccgtt ttgaacgtc 
      cagaacttg ttttgggca cttatgagt tcgttgcaa 
      ctcctgaat attgtcgga ataatactt tacctaaac 
      caccagcta tcattttcg ttattgatg tttcttaca 
      ataacatta aatcaggtt tagtacatt tattatgtg 
      cacaaatta aggagatta tccagcatt taccggtgg 
      atcaaggac aggaaaaaa gcaatatgc gagttgggt 
      ttattacat acaacagct cagtgcatt tttacaaca 
      ttaagggga acttggctg ctcctcaaa tgctaattg 
      ttggtgaca tccaagaaa tgtttatgc cccttttgg 
      acggatttt tatgcacac cggaagaat ccggtcctg 
      ttatcgacg cattgatgc aacaaattc cagcaatgt 
      ttggtgaca caagagaaa cagaaacca tacttttac 
      cggcgattt tagcaggac tagaggctt taaaagcgt 
      ccgaatgtg cgggaaaat tatgcgttt tccattcga 
      gtttgccaa cagctgaag gacctggaa aattgaaaa 
      atcgtgacg atcgtaaat tactaggaa ctgataaag 
      aacgaactg ttttaacac cacaaacac aagcctata 
      atcagttag gtcaggatt gtgtagctg ccggttgtt 
      cagttgatc tttttattt ttaataacg catacattg 
      atattgcaa caatcagtc aagtgtcga ggttgacag 
      ggggcgaag ttcacaaat atacatatt tccaggccg 
      atattgatg gtgagagat taataaaag atattataa 
      cgaatatca gcagaccta tcgcatttg atgctatta 
      tgagagtac ggacgtcaa ctggtgtca gacccactg 
      atttttatg ggcattttt acatgtcaa ataccacag 
      atatggaac tagcagcca ttgattgtg ataaagcag 
      tggctgttg aaataaaac acgacgaca aactatctg 
      aagaagaag gtgtttaca tccaggcgg ctttattgt 
      atacgtcat gttcgggtc aacgccgcc tgcgcgtaa 
      tgaatcttt cactgaaaa cttgctcac aaatgtccg 
      acctttacc gaaactgtg attgtgata cgataatca 
      attacgttt cgaaacagg caacttata aattatacg 
      aacataatc cgaaagctg taaaagaca gtatagtga 
      cacgagctg cgcaaattt tagcaagct atcgcaaaa 
      actgtgcta gtccaagta gcgcaggtc agttgattt 
      tgcccgaat gtatgaaat tattgcctt tgtatgtga 
      attgtctat tgaagagcg atgctctgt ctggaggct 
      ctgatatga caatagacg atcggtcgt atgttatgc 
      aggaggtca tgattatgg atataccca cgagtgtga 
      attatttct atccaaggt tgattcctt tgattgatt 
      tggatgtga gtcaagatc ctgttcaga ttccttcgc 
      ctattaggt gtacgatag ataaaatga gtgagcaag 
      gtgtttata tcttggaaa atgggatac acatgtttt 
      tgtggattg gacttgaag ccaatcctg atttcatcc 
      agaaagttt ttggagtcc cgtctgcca ttcaagtgg 
      atgtggaaa aagttgcat tgcctgaat tggataatt 
      cgttgtcgg ttgctgtaa gaaatatta ttgaagaaa 
      tcaggatac agagacata aatgcatga ggttaacta 
      ttgttcgtc agagagaga agttggagc ccgttttta 
      aacatttcc tagctgaag atcgtggaa cggatggat 
      ctccgagtt atgtcgact ttttaatac atatgcaca 
      aagaaattc gttcgttgt taagctagc aattgagaa 
      gaaaatgaa tttattttc atcatttat tttacttga 
      aatatacta tttttatta ttttttaag gagaaggaa 
      atgtgcgga ttcttgtac tggaaaata aataaaaat 
      acagtttcc acttgaatt attatgtta caatgttac 
      tgtagattg ttattttac ttaaacttg aatgtaaat 
      gtttaatac aacataagc taaagtatg gaattattt 
      tgtatttat atttattgg tgcccattt gagatcatg 
      tatcatact aatacctaa taaatagct gtaagaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY