Status
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Gene information
General information
TC034995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 161026..217921
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034995
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01101

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034995 RA
      MVLEPKFVD YGSIHPTAD VPQLQQHNS SHALLSPRS 
      VLSLPPLDD EEPPNTSKG TNMTQGDLY QRQPLLPNP 
      GKSDKWGHD AHNSSGQEY YEDEDDIIQ KTNESNGVI 
      KINMPPPIR EEPRFPQER WKTLLAAII MAFNFVLTL 
      TSLSLVHER VPDRKVYGP LPDVFLDNV PAFDWALDV 
      SEYIIIVCV NSCIISIFF HKHRFIVFR RIFLIMSLL 
      YMYRSITMY VTVLPIASK TYFCSPKSN ETSPLTIAK 
      RVLSLMSGF GLSVNGKHV YCGDFIYSG HTVILVLSY 
      LFIAEYTPK KLYPLHWFY WVLGIVGVV MLQLSHGHY 
      TVDVIIAYY VTTRIFWTY HTLANNVGL KQHSPNNLL 
      GREWWYCFF LYFEGNVGG PIPRQFDWP LPWPRRFHS 
      KSRDS
    • >TC034995 RB
      MIRPSCVDD NTCLLRHCV GDGDETQAH LSWSSWSST 
      PGFNPSPKK SVRLLPVPR VTASRTQFE SNRLQHDPV 
      NTGIFIVNS TFGHCIMVK WCAVSTWDY FRMVLEPKF 
      VDYGSIHPT ADVPQLQQH NSSHALLSP RSVLSLPPL 
      DDEEPPNTS KGTNMTQGD LYQRQPLLP NPGKSDKWG 
      HDAHNSSGQ EYYEDEDDI IQKTNESNG VIKINMPPP 
      IREEPRFPQ ERWKTLLAA IIMAFNFVL TLTSLSLVH 
      ERVPDRKVY GPLPDVFLD NVPAFDWAL DVSEYIIIV 
      CVNSCIISI FFHKHRFIV FRRIFLIMS LLYMYRSIT 
      MYVTVLPIA SKTYFCSPK SNETSPLTI AKRVLSLMS 
      GFGLSVNGK HVYCGDFIY SGHTVILVL SYLFIAEYT 
      PKKLYPLHW FYWVLGIVG VVMLQLSHG HYTVDVIIA 
      YYVTTRIFW TYHTLANNV GLKQHSPNN LLGREWWYC 
      FFLYFEGNV GGPIPRQFD WPLPWPRRF HSKSRDS
    CDS sequence
    • >TC034995 RA
      atggtatta gagcccaag ttcgttgac tatggtagt 
      atccatcca acagcagat gtgcctcag cttcaacag 
      cataactct tcacatgcc ttattgagc ccacgctca 
      gtcctatct ctacctcct ctcgacgac gaggaaccg 
      ccaaatacc tccaaggga acgaatatg acgcaagga 
      gatttatat caaagacag cctttattg cctaatccg 
      ggaaagtcc gataaatgg ggccacgac gcacacaat 
      tcctccggc caggagtac tacgaggac gaagacgat 
      atcattcaa aaaacaaac gagagcaat ggcgttatc 
      aaaatcaac atgccacct cctatacgg gaagaaccg 
      aggttcccg caggaacga tggaaaacc ctgttagct 
      gcgataatt atggccttt aacttcgta ctgacgctc 
      acgtctttg tcacttgta catgaacgg gtacccgat 
      cgaaaggta tatggtccc cttcctgat gtgttcctc 
      gacaacgta cctgctttc gactgggct ttagatgta 
      tcagagtat attatcata gtttgtgta aattcatgt 
      atcatctct atctttttt cacaagcat aggtttata 
      gtatttagg cggatattt ttaataatg tctctactg 
      tacatgtac cgatccatc accatgtac gtcacggtg 
      ctaccaatc gcaagtaaa acgtatttt tgttcacca 
      aaatcaaac gaaacgtct cctttaacg atcgccaag 
      cgagtcctt agcctcatg tccgggttc ggtttgtcc 
      gtgaacggt aaacacgtt tattgcggg gattttatc 
      tacagcggc catacagtc attttagta cttagttac 
      ctcttcatt gcagaatac actccgaaa aaattatat 
      cccctacat tggttttat tgggtgttg ggaatcgtg 
      ggggttgtg atgcttcaa ctgtcacac ggacactac 
      acggtggac gtcatcata gcgtattat gttacaact 
      aggattttt tggacttat cacacactt gccaacaac 
      gtcgggctt aaacaacat tcgccaaat aatttgttg 
      ggccgcgag tggtggtac tgtttcttt ctgtatttc 
      gaagggaac gttggaggt cccatcccg agacagttc 
      gactggccc ctgccgtgg cctcggcgg tttcactcg 
      aaaagtcgg gatagttag
    • >TC034995 RB
      atgattcga cctagttgc gtggatgac aacacctgt 
      ttgcttcga cactgtgtt ggcgacgga gacgaaacg 
      caagcgcac ctgtcctgg tcgtcctgg tcgtcaaca 
      ccaggattt aacccatcg cccaagaag tccgtccgt 
      ctccttcca gtgccgcgc gtaactgct agcaggaca 
      cagttcgaa tcgaatcga ctacaacat gacccagta 
      aataccggt atttttatt gttaacagc acctttgga 
      cactgcata atggtgaag tggtgtgct gttagcact 
      tgggattat tttagaatg gtattagag cccaagttc 
      gttgactat ggtagtatc catccaaca gcagatgtg 
      cctcagctt caacagcat aactcttca catgcctta 
      ttgagccca cgctcagtc ctatctcta cctcctctc 
      gacgacgag gaaccgcca aatacctcc aagggaacg 
      aatatgacg caaggagat ttatatcaa agacagcct 
      ttattgcct aatccggga aagtccgat aaatggggc 
      cacgacgca cacaattcc tccggccag gagtactac 
      gaggacgaa gacgatatc attcaaaaa acaaacgag 
      agcaatggc gttatcaaa atcaacatg ccacctcct 
      atacgggaa gaaccgagg ttcccgcag gaacgatgg 
      aaaaccctg ttagctgcg ataattatg gcctttaac 
      ttcgtactg acgctcacg tctttgtca cttgtacat 
      gaacgggta cccgatcga aaggtatat ggtcccctt 
      cctgatgtg ttcctcgac aacgtacct gctttcgac 
      tgggcttta gatgtatca gagtatatt atcatagtt 
      tgtgtaaat tcatgtatc atctctatc ttttttcac 
      aagcatagg tttatagta tttaggcgg atattttta 
      ataatgtct ctactgtac atgtaccga tccatcacc 
      atgtacgtc acggtgcta ccaatcgca agtaaaacg 
      tatttttgt tcaccaaaa tcaaacgaa acgtctcct 
      ttaacgatc gccaagcga gtccttagc ctcatgtcc 
      gggttcggt ttgtccgtg aacggtaaa cacgtttat 
      tgcggggat tttatctac agcggccat acagtcatt 
      ttagtactt agttacctc ttcattgca gaatacact 
      ccgaaaaaa ttatatccc ctacattgg ttttattgg 
      gtgttggga atcgtgggg gttgtgatg cttcaactg 
      tcacacgga cactacacg gtggacgtc atcatagcg 
      tattatgtt acaactagg attttttgg acttatcac 
      acacttgcc aacaacgtc gggcttaaa caacattcg 
      ccaaataat ttgttgggc cgcgagtgg tggtactgt 
      ttctttctg tatttcgaa gggaacgtt ggaggtccc 
      atcccgaga cagttcgac tggcccctg ccgtggcct 
      cggcggttt cactcgaaa agtcgggat agttag
    mRNA sequence
    • >TC034995 RA
      cgcgcagac gggcatgtt agtggcatt cgtttcaat 
      ctcgcgtct ttatttttt ttctttagt aaaaacgtc 
      aaaaaagtg tcagtggtg acatcgcga ctaaattaa 
      agcacatgt tttaaatat cgggcgcta cattttccg 
      ggcgagaaa tttcttttg acaaaattt aggttatgt 
      gtgaaatgc acctgtcgt acgttgttg ctgtctcgg 
      cgaaagaaa gcgaagtca tctggtacg ctaattgag 
      gaggtttat tggcaagtt caaagttct tctctaccc 
      tggatggaa aaagtgcgg atacaatca tcgttcagc 
      tatgtctag ttgacaagc accggattt gttagaatg 
      gtattagag cccaagttc gttgactat ggtagtatc 
      catccaaca gcagatgtg cctcagctt caacagcat 
      aactcttca catgcctta ttgagccca cgctcagtc 
      ctatctcta cctcctctc gacgacgag gaaccgcca 
      aatacctcc aagggaacg aatatgacg caaggagat 
      ttatatcaa agacagcct ttattgcct aatccggga 
      aagtccgat aaatggggc cacgacgca cacaattcc 
      tccggccag gagtactac gaggacgaa gacgatatc 
      attcaaaaa acaaacgag agcaatggc gttatcaaa 
      atcaacatg ccacctcct atacgggaa gaaccgagg 
      ttcccgcag gaacgatgg aaaaccctg ttagctgcg 
      ataattatg gcctttaac ttcgtactg acgctcacg 
      tctttgtca cttgtacat gaacgggta cccgatcga 
      aaggtatat ggtcccctt cctgatgtg ttcctcgac 
      aacgtacct gctttcgac tgggcttta gatgtatca 
      gagtatatt atcatagtt tgtgtaaat tcatgtatc 
      atctctatc ttttttcac aagcatagg tttatagta 
      tttaggcgg atattttta ataatgtct ctactgtac 
      atgtaccga tccatcacc atgtacgtc acggtgcta 
      ccaatcgca agtaaaacg tatttttgt tcaccaaaa 
      tcaaacgaa acgtctcct ttaacgatc gccaagcga 
      gtccttagc ctcatgtcc gggttcggt ttgtccgtg 
      aacggtaaa cacgtttat tgcggggat tttatctac 
      agcggccat acagtcatt ttagtactt agttacctc 
      ttcattgca gaatacact ccgaaaaaa ttatatccc 
      ctacattgg ttttattgg gtgttggga atcgtgggg 
      gttgtgatg cttcaactg tcacacgga cactacacg 
      gtggacgtc atcatagcg tattatgtt acaactagg 
      attttttgg acttatcac acacttgcc aacaacgtc 
      gggcttaaa caacattcg ccaaataat ttgttgggc 
      cgcgagtgg tggtactgt ttctttctg tatttcgaa 
      gggaacgtt ggaggtccc atcccgaga cagttcgac 
      tggcccctg ccgtggcct cggcggttt cactcgaaa 
      agtcgggat agttagtgc agtgtgtgt ttgaaaaag 
      tggtggaga actttatca aattttatt atatgtgat 
      aaaaataat gtgtagcgc cttaaactg acttgctct 
      ttatacata tttatctat ttacctata catactgtt 
      agatctttc tgatatttt tattacatc agtgtattt 
      cagtggaac agcaggaca taaacggtt ttacaaggg 
      tgggttcat gatgtattt tttgagttg cattttcgg 
      aacgtcttt tgtatttta gttaggact gccgcgagc 
      agaacacac gatttaata aggaaacga aattaaagc 
      aaaattgtg ccaagtaac gtaagaatc tctgaaaag 
      tgaaaaaac tttttaatg taattttct tattaaatc 
      tttcgattg aatcaaagt tccattttt aatttgtga 
      taataacag tagaatgca gtgttttta aatgaaatt 
      gttagctag gaacctatt ttgttacca gtgtacaat 
      gaagactac acttacata tttacagac tttgcgann 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnn
    • >TC034995 RB
      tacactaaa aaatgattc gacctagtt gcgtggatg 
      acaacacct gtttgcttc gacactgtg ttggcgacg 
      gagacgaaa cgcaagcgc acctgtcct ggtcgtcct 
      ggtcgtcaa caccaggat ttaacccat cgcccaaga 
      agtccgtcc gtctccttc cagtgccgc gcgtaactg 
      ctagcagga cacagttcg aatcgaatc gactacaac 
      atgacccag taaataccg gtattttta ttgttaaca 
      gcacctttg gacactgca taatggtga agtggtgtg 
      ctgttagca cttgggatt attttagaa tggtattag 
      agcccaagt tcgttgact atggtagta tccatccaa 
      cagcagatg tgcctcagc ttcaacagc ataactctt 
      cacatgcct tattgagcc cacgctcag tcctatctc 
      tacctcctc tcgacgacg aggaaccgc caaatacct 
      ccaagggaa cgaatatga cgcaaggag atttatatc 
      aaagacagc ctttattgc ctaatccgg gaaagtccg 
      ataaatggg gccacgacg cacacaatt cctccggcc 
      aggagtact acgaggacg aagacgata tcattcaaa 
      aaacaaacg agagcaatg gcgttatca aaatcaaca 
      tgccacctc ctatacggg aagaaccga ggttcccgc 
      aggaacgat ggaaaaccc tgttagctg cgataatta 
      tggccttta acttcgtac tgacgctca cgtctttgt 
      cacttgtac atgaacggg tacccgatc gaaaggtat 
      atggtcccc ttcctgatg tgttcctcg acaacgtac 
      ctgctttcg actgggctt tagatgtat cagagtata 
      ttatcatag tttgtgtaa attcatgta tcatctcta 
      tcttttttc acaagcata ggtttatag tatttaggc 
      ggatatttt taataatgt ctctactgt acatgtacc 
      gatccatca ccatgtacg tcacggtgc taccaatcg 
      caagtaaaa cgtattttt gttcaccaa aatcaaacg 
      aaacgtctc ctttaacga tcgccaagc gagtcctta 
      gcctcatgt ccgggttcg gtttgtccg tgaacggta 
      aacacgttt attgcgggg attttatct acagcggcc 
      atacagtca ttttagtac ttagttacc tcttcattg 
      cagaataca ctccgaaaa aattatatc ccctacatt 
      ggttttatt gggtgttgg gaatcgtgg gggttgtga 
      tgcttcaac tgtcacacg gacactaca cggtggacg 
      tcatcatag cgtattatg ttacaacta ggatttttt 
      ggacttatc acacacttg ccaacaacg tcgggctta 
      aacaacatt cgccaaata atttgttgg gccgcgagt 
      ggtggtact gtttctttc tgtatttcg aagggaacg 
      ttggaggtc ccatcccga gacagttcg actggcccc 
      tgccgtggc ctcggcggt ttcactcga aaagtcggg 
      atagttagt gcagtgtgt gtttgaaaa agtggtgga 
      gaactttat caaatttta ttatatgtg ataaaaata 
      atgtgtagc gccttaaac tgacttgct ctttataca 
      tatttatct atttaccta tacatactg ttagatctt 
      tctgatatt tttattaca tcagtgtat ttcagtgga 
      acagcagga cataaacgg ttttacaag ggtgggttc 
      atgatgtat tttttgagt tgcattttc ggaacgtct 
      tttgtattt tagttagga ctgccgcga gcagaacac 
      acgatttaa taaggaaac gaaattaaa gcaaaattg 
      tgccaagta acgtaagaa tctctgaaa agtgaaaaa 
      actttttaa tgtaatttt cttattaaa tctttcgat 
      tgaatcaaa gttccattt ttaatttgt gataataac 
      agtagaatg cagtgtttt taaatgaaa ttgttagct 
      aggaaccta ttttgttac cagtgtaca atgaagact 
      acacttaca tatttacag actttgcga nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnn
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