| General information | |||
| TC034995 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451448.1: 161026..217921 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034995 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01101 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034995 RA
MVLEPKFVD YGSIHPTAD VPQLQQHNS SHALLSPRS VLSLPPLDD EEPPNTSKG TNMTQGDLY QRQPLLPNP GKSDKWGHD AHNSSGQEY YEDEDDIIQ KTNESNGVI KINMPPPIR EEPRFPQER WKTLLAAII MAFNFVLTL TSLSLVHER VPDRKVYGP LPDVFLDNV PAFDWALDV SEYIIIVCV NSCIISIFF HKHRFIVFR RIFLIMSLL YMYRSITMY VTVLPIASK TYFCSPKSN ETSPLTIAK RVLSLMSGF GLSVNGKHV YCGDFIYSG HTVILVLSY LFIAEYTPK KLYPLHWFY WVLGIVGVV MLQLSHGHY TVDVIIAYY VTTRIFWTY HTLANNVGL KQHSPNNLL GREWWYCFF LYFEGNVGG PIPRQFDWP LPWPRRFHS KSRDS
- >TC034995 RB
MIRPSCVDD NTCLLRHCV GDGDETQAH LSWSSWSST PGFNPSPKK SVRLLPVPR VTASRTQFE SNRLQHDPV NTGIFIVNS TFGHCIMVK WCAVSTWDY FRMVLEPKF VDYGSIHPT ADVPQLQQH NSSHALLSP RSVLSLPPL DDEEPPNTS KGTNMTQGD LYQRQPLLP NPGKSDKWG HDAHNSSGQ EYYEDEDDI IQKTNESNG VIKINMPPP IREEPRFPQ ERWKTLLAA IIMAFNFVL TLTSLSLVH ERVPDRKVY GPLPDVFLD NVPAFDWAL DVSEYIIIV CVNSCIISI FFHKHRFIV FRRIFLIMS LLYMYRSIT MYVTVLPIA SKTYFCSPK SNETSPLTI AKRVLSLMS GFGLSVNGK HVYCGDFIY SGHTVILVL SYLFIAEYT PKKLYPLHW FYWVLGIVG VVMLQLSHG HYTVDVIIA YYVTTRIFW TYHTLANNV GLKQHSPNN LLGREWWYC FFLYFEGNV GGPIPRQFD WPLPWPRRF HSKSRDS
- >TC034995 RA
atggtatta gagcccaag ttcgttgac tatggtagt atccatcca acagcagat gtgcctcag cttcaacag cataactct tcacatgcc ttattgagc ccacgctca gtcctatct ctacctcct ctcgacgac gaggaaccg ccaaatacc tccaaggga acgaatatg acgcaagga gatttatat caaagacag cctttattg cctaatccg ggaaagtcc gataaatgg ggccacgac gcacacaat tcctccggc caggagtac tacgaggac gaagacgat atcattcaa aaaacaaac gagagcaat ggcgttatc aaaatcaac atgccacct cctatacgg gaagaaccg aggttcccg caggaacga tggaaaacc ctgttagct gcgataatt atggccttt aacttcgta ctgacgctc acgtctttg tcacttgta catgaacgg gtacccgat cgaaaggta tatggtccc cttcctgat gtgttcctc gacaacgta cctgctttc gactgggct ttagatgta tcagagtat attatcata gtttgtgta aattcatgt atcatctct atctttttt cacaagcat aggtttata gtatttagg cggatattt ttaataatg tctctactg tacatgtac cgatccatc accatgtac gtcacggtg ctaccaatc gcaagtaaa acgtatttt tgttcacca aaatcaaac gaaacgtct cctttaacg atcgccaag cgagtcctt agcctcatg tccgggttc ggtttgtcc gtgaacggt aaacacgtt tattgcggg gattttatc tacagcggc catacagtc attttagta cttagttac ctcttcatt gcagaatac actccgaaa aaattatat cccctacat tggttttat tgggtgttg ggaatcgtg ggggttgtg atgcttcaa ctgtcacac ggacactac acggtggac gtcatcata gcgtattat gttacaact aggattttt tggacttat cacacactt gccaacaac gtcgggctt aaacaacat tcgccaaat aatttgttg ggccgcgag tggtggtac tgtttcttt ctgtatttc gaagggaac gttggaggt cccatcccg agacagttc gactggccc ctgccgtgg cctcggcgg tttcactcg aaaagtcgg gatagttag
- >TC034995 RB
atgattcga cctagttgc gtggatgac aacacctgt ttgcttcga cactgtgtt ggcgacgga gacgaaacg caagcgcac ctgtcctgg tcgtcctgg tcgtcaaca ccaggattt aacccatcg cccaagaag tccgtccgt ctccttcca gtgccgcgc gtaactgct agcaggaca cagttcgaa tcgaatcga ctacaacat gacccagta aataccggt atttttatt gttaacagc acctttgga cactgcata atggtgaag tggtgtgct gttagcact tgggattat tttagaatg gtattagag cccaagttc gttgactat ggtagtatc catccaaca gcagatgtg cctcagctt caacagcat aactcttca catgcctta ttgagccca cgctcagtc ctatctcta cctcctctc gacgacgag gaaccgcca aatacctcc aagggaacg aatatgacg caaggagat ttatatcaa agacagcct ttattgcct aatccggga aagtccgat aaatggggc cacgacgca cacaattcc tccggccag gagtactac gaggacgaa gacgatatc attcaaaaa acaaacgag agcaatggc gttatcaaa atcaacatg ccacctcct atacgggaa gaaccgagg ttcccgcag gaacgatgg aaaaccctg ttagctgcg ataattatg gcctttaac ttcgtactg acgctcacg tctttgtca cttgtacat gaacgggta cccgatcga aaggtatat ggtcccctt cctgatgtg ttcctcgac aacgtacct gctttcgac tgggcttta gatgtatca gagtatatt atcatagtt tgtgtaaat tcatgtatc atctctatc ttttttcac aagcatagg tttatagta tttaggcgg atattttta ataatgtct ctactgtac atgtaccga tccatcacc atgtacgtc acggtgcta ccaatcgca agtaaaacg tatttttgt tcaccaaaa tcaaacgaa acgtctcct ttaacgatc gccaagcga gtccttagc ctcatgtcc gggttcggt ttgtccgtg aacggtaaa cacgtttat tgcggggat tttatctac agcggccat acagtcatt ttagtactt agttacctc ttcattgca gaatacact ccgaaaaaa ttatatccc ctacattgg ttttattgg gtgttggga atcgtgggg gttgtgatg cttcaactg tcacacgga cactacacg gtggacgtc atcatagcg tattatgtt acaactagg attttttgg acttatcac acacttgcc aacaacgtc gggcttaaa caacattcg ccaaataat ttgttgggc cgcgagtgg tggtactgt ttctttctg tatttcgaa gggaacgtt ggaggtccc atcccgaga cagttcgac tggcccctg ccgtggcct cggcggttt cactcgaaa agtcgggat agttag
- >TC034995 RA
cgcgcagac gggcatgtt agtggcatt cgtttcaat ctcgcgtct ttatttttt ttctttagt aaaaacgtc aaaaaagtg tcagtggtg acatcgcga ctaaattaa agcacatgt tttaaatat cgggcgcta cattttccg ggcgagaaa tttcttttg acaaaattt aggttatgt gtgaaatgc acctgtcgt acgttgttg ctgtctcgg cgaaagaaa gcgaagtca tctggtacg ctaattgag gaggtttat tggcaagtt caaagttct tctctaccc tggatggaa aaagtgcgg atacaatca tcgttcagc tatgtctag ttgacaagc accggattt gttagaatg gtattagag cccaagttc gttgactat ggtagtatc catccaaca gcagatgtg cctcagctt caacagcat aactcttca catgcctta ttgagccca cgctcagtc ctatctcta cctcctctc gacgacgag gaaccgcca aatacctcc aagggaacg aatatgacg caaggagat ttatatcaa agacagcct ttattgcct aatccggga aagtccgat aaatggggc cacgacgca cacaattcc tccggccag gagtactac gaggacgaa gacgatatc attcaaaaa acaaacgag agcaatggc gttatcaaa atcaacatg ccacctcct atacgggaa gaaccgagg ttcccgcag gaacgatgg aaaaccctg ttagctgcg ataattatg gcctttaac ttcgtactg acgctcacg tctttgtca cttgtacat gaacgggta cccgatcga aaggtatat ggtcccctt cctgatgtg ttcctcgac aacgtacct gctttcgac tgggcttta gatgtatca gagtatatt atcatagtt tgtgtaaat tcatgtatc atctctatc ttttttcac aagcatagg tttatagta tttaggcgg atattttta ataatgtct ctactgtac atgtaccga tccatcacc atgtacgtc acggtgcta ccaatcgca agtaaaacg tatttttgt tcaccaaaa tcaaacgaa acgtctcct ttaacgatc gccaagcga gtccttagc ctcatgtcc gggttcggt ttgtccgtg aacggtaaa cacgtttat tgcggggat tttatctac agcggccat acagtcatt ttagtactt agttacctc ttcattgca gaatacact ccgaaaaaa ttatatccc ctacattgg ttttattgg gtgttggga atcgtgggg gttgtgatg cttcaactg tcacacgga cactacacg gtggacgtc atcatagcg tattatgtt acaactagg attttttgg acttatcac acacttgcc aacaacgtc gggcttaaa caacattcg ccaaataat ttgttgggc cgcgagtgg tggtactgt ttctttctg tatttcgaa gggaacgtt ggaggtccc atcccgaga cagttcgac tggcccctg ccgtggcct cggcggttt cactcgaaa agtcgggat agttagtgc agtgtgtgt ttgaaaaag tggtggaga actttatca aattttatt atatgtgat aaaaataat gtgtagcgc cttaaactg acttgctct ttatacata tttatctat ttacctata catactgtt agatctttc tgatatttt tattacatc agtgtattt cagtggaac agcaggaca taaacggtt ttacaaggg tgggttcat gatgtattt tttgagttg cattttcgg aacgtcttt tgtatttta gttaggact gccgcgagc agaacacac gatttaata aggaaacga aattaaagc aaaattgtg ccaagtaac gtaagaatc tctgaaaag tgaaaaaac tttttaatg taattttct tattaaatc tttcgattg aatcaaagt tccattttt aatttgtga taataacag tagaatgca gtgttttta aatgaaatt gttagctag gaacctatt ttgttacca gtgtacaat gaagactac acttacata tttacagac tttgcgann nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnn
- >TC034995 RB
tacactaaa aaatgattc gacctagtt gcgtggatg acaacacct gtttgcttc gacactgtg ttggcgacg gagacgaaa cgcaagcgc acctgtcct ggtcgtcct ggtcgtcaa caccaggat ttaacccat cgcccaaga agtccgtcc gtctccttc cagtgccgc gcgtaactg ctagcagga cacagttcg aatcgaatc gactacaac atgacccag taaataccg gtattttta ttgttaaca gcacctttg gacactgca taatggtga agtggtgtg ctgttagca cttgggatt attttagaa tggtattag agcccaagt tcgttgact atggtagta tccatccaa cagcagatg tgcctcagc ttcaacagc ataactctt cacatgcct tattgagcc cacgctcag tcctatctc tacctcctc tcgacgacg aggaaccgc caaatacct ccaagggaa cgaatatga cgcaaggag atttatatc aaagacagc ctttattgc ctaatccgg gaaagtccg ataaatggg gccacgacg cacacaatt cctccggcc aggagtact acgaggacg aagacgata tcattcaaa aaacaaacg agagcaatg gcgttatca aaatcaaca tgccacctc ctatacggg aagaaccga ggttcccgc aggaacgat ggaaaaccc tgttagctg cgataatta tggccttta acttcgtac tgacgctca cgtctttgt cacttgtac atgaacggg tacccgatc gaaaggtat atggtcccc ttcctgatg tgttcctcg acaacgtac ctgctttcg actgggctt tagatgtat cagagtata ttatcatag tttgtgtaa attcatgta tcatctcta tcttttttc acaagcata ggtttatag tatttaggc ggatatttt taataatgt ctctactgt acatgtacc gatccatca ccatgtacg tcacggtgc taccaatcg caagtaaaa cgtattttt gttcaccaa aatcaaacg aaacgtctc ctttaacga tcgccaagc gagtcctta gcctcatgt ccgggttcg gtttgtccg tgaacggta aacacgttt attgcgggg attttatct acagcggcc atacagtca ttttagtac ttagttacc tcttcattg cagaataca ctccgaaaa aattatatc ccctacatt ggttttatt gggtgttgg gaatcgtgg gggttgtga tgcttcaac tgtcacacg gacactaca cggtggacg tcatcatag cgtattatg ttacaacta ggatttttt ggacttatc acacacttg ccaacaacg tcgggctta aacaacatt cgccaaata atttgttgg gccgcgagt ggtggtact gtttctttc tgtatttcg aagggaacg ttggaggtc ccatcccga gacagttcg actggcccc tgccgtggc ctcggcggt ttcactcga aaagtcggg atagttagt gcagtgtgt gtttgaaaa agtggtgga gaactttat caaatttta ttatatgtg ataaaaata atgtgtagc gccttaaac tgacttgct ctttataca tatttatct atttaccta tacatactg ttagatctt tctgatatt tttattaca tcagtgtat ttcagtgga acagcagga cataaacgg ttttacaag ggtgggttc atgatgtat tttttgagt tgcattttc ggaacgtct tttgtattt tagttagga ctgccgcga gcagaacac acgatttaa taaggaaac gaaattaaa gcaaaattg tgccaagta acgtaagaa tctctgaaa agtgaaaaa actttttaa tgtaatttt cttattaaa tctttcgat tgaatcaaa gttccattt ttaatttgt gataataac agtagaatg cagtgtttt taaatgaaa ttgttagct aggaaccta ttttgttac cagtgtaca atgaagact acacttaca tatttacag actttgcga nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnn
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
