Status
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Gene information
General information
TC006905 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451351.1: 4063..7264
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC006905
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01100

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      Fertility
      egg chamber mostly not present - Number of animals showing the phenotype: 4

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
      Days post injection

      larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection

      pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
      Days post injection

      iBeetle screen iB_06779

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection

      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      Adult morphology
      prothoracic odoriferous glands darker - Number of animals showing the phenotype: 2 (13 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006905 RA
        MEDNPAKRL KSTLSPVFE DVLKSDTFV DKSALMLAV 
FETDDKPMV LCTAPRRFG KTVNLSMMQ RFLELEVDE 
NGEEKTKVL KEKDNYKLF SSARFKLKV MENTKFVET 
HFGQYPIIE INFLCCDDV TSKSDAVNV CRSAISEAF 
RQHSYLYES KLSPLKDYE KKHCEQWCD EFTYTGIPD 
DKVIKGLGC LAAYLNKFH KRMVFLLVD EYDSIISKA 
LYKVKNADP ETELEELEN AASFPIEVI GYALKKIPG 
IFKGAYITG ILDVGGVAI STLLGNVLR RQFGTNNLC 
QDFYGFTED EVCTLFNRF AVKKELREK AKAKYNGYL 
NGSKQCLYN PQTIVQFLG NLSLGEGAL KNYWQQSGY 
IHNMYKLLE EGSVRRLLY ELLQGVKED FQFTLKPLD 
LSDLKSLHN ILAKQSLTC REEIVVRFL VSLGYLTYS 
FDDHKLQIP IPELIDEFM KLLRAYFFE RFGINRELA 
EDCSASFSK LTVLCGEGD LLAKFKEEL ANFQNSITC 
LLQRSTLID PLNEATLQS IITLTCSKA GFLFGNEIT 
IPIHRTKVD IVLFNDKSG IIMELKCGP NTVQDAFKQ 
IFQNRYYNV FTNKKYFDE RKKSPSLSF VMAMHVDSD 
NKGSVMCLR REDVIHVLD DLQLDARES SLEGRIEIA 
VNSMGEPFI NTCCESAIL VS
      CDS sequence
      • >TC006905 RA
        atggaggat aatccggcg aaacgttta aaatctacc 
ctttcaccg gtttttgaa gatgtcttg aaatccgat 
acttttgtt gataaaagt gcgttgatg ttagcagtt 
tttgaaacg gacgataaa cctatggta ttgtgtacc 
gcaccgcgc cgatttggc aaaactgta aacttatcc 
atgatgcaa cgattcttg gagttggaa gtagatgaa 
aatggagag gagaaaaca aaggttttg aaagaaaaa 
gacaattac aaactgttc agttcagca aggttcaag 
ttaaaagtc atggagaat acgaagttt gtagaaact 
catttcgga caatatccg ataatcgaa ataaatttt 
ctctgttgt gatgacgtg acaagcaaa tctgatgca 
gtgaatgtg tgtagaagc gctatatcg gaagctttt 
aggcaacac agttacctt tacgaatcc aaattgtca 
ccgttgaaa gactatgaa aaaaagcat tgcgaacaa 
tggtgtgac gaatttacc tacacagga attccagat 
gacaaagta atcaagggg ttgggatgc ttggcagct 
tatctgaat aagtttcat aaaagaatg gtttttcta 
ctcgttgac gagtacgac tcgatcatt tcaaaagcg 
ttgtataaa gtgaagaac gctgatcca gagactgag 
cttgaagag ctagagaat gctgcctca ttcccaatt 
gaagtaatt ggatatgct ctaaaaaaa attcctggg 
atctttaaa ggagcgtat attaccgga attttggat 
gtgggtggt gtagcgatt tccacccta ttgggtaat 
gtattgcga cggcaattc ggaaccaac aatctgtgc 
caagatttc tatggattc actgaagat gaagtttgt 
actctattt aatcggttt gcagtaaag aaggaattg 
agagaaaaa gcgaaggct aaatataat gggtattta 
aatggctcg aaacaatgc ctatataat cctcaaacg 
attgtgcag tttttggga aatttatct ctgggagaa 
ggtgcttta aaaaattat tggcaacaa tctggttac 
attcacaat atgtataaa ctacttgaa gagggtagc 
gtgagaagg ttgttgtat gaacttcta caaggtgtt 
aaagaagat tttcaattc acgttaaaa ccgttagac 
ttaagtgat ctgaaatct ttgcataac atattagct 
aaacaaagt ttgacttgt cgcgaagaa atagttgtt 
cgatttctt gtatctcta ggatactta acatattca 
tttgatgat cacaaactc caaatacct atccctgag 
ctaattgat gaatttatg aagctgcta cgagcttat 
ttttttgaa agatttggt attaacaga gaattggct 
gaagattgt tcagcatca ttttctaag ctaaccgtt 
ctctgtggt gaaggtgat ttattagca aagttcaaa 
gaagaatta gcaaatttt caaaatagt ataacctgt 
ctacttcaa cgttcaaca cttatcgat ccattgaat 
gaggcaact ttacagagt ataattaca ttgacatgt 
agtaaagca ggatttctc ttcggaaac gaaataaca 
attccaatt catagaacc aaagttgat atagtactg 
tttaatgat aaatctggg ataataatg gaattaaaa 
tgtggtcca aatactgta caagacgca tttaaacaa 
atctttcaa aatcggtac tacaatgtt ttcacaaat 
aagaagtat tttgatgaa cgtaagaaa tctccttct 
ctttctttt gttatggca atgcatgtg gattctgac 
aacaaagga tctgtgatg tgcctgcga cgtgaggat 
gttattcac gttctggat gatctgcaa cttgacgct 
agagagtca tcgctagaa ggtcgcatt gagatagct 
gttaattct atgggtgaa cctttcatt aacacttgt 
tgcgaaagt gcaattctt gtctcttaa
      mRNA sequence
      • >TC006905 RA
        tgtttattt ttttctgtt ccgtataaa agatttaaa 
actgttttt gaattgttg gtgtgcttg ttaacagtg 
tgtagttaa tactatggg tcaatcttg cttgcttta 
ttttttaca atttagtaa tgaaaaaca gtatttttc 
gatttattg tgcaaacat taattcgtg gttttcatt 
cattgttac aggcagttt gtttattat ggaggataa 
tccggcgaa acgtttaaa atctaccct ttcaccggt 
ttttgaaga tgtcttgaa atccgatac ttttgttga 
taaaagtgc gttgatgtt agcagtttt tgaaacgga 
cgataaacc tatggtatt gtgtaccgc accgcgccg 
atttggcaa aactgtaaa cttatccat gatgcaacg 
attcttgga gttggaagt agatgaaaa tggagagga 
gaaaacaaa ggttttgaa agaaaaaga caattacaa 
actgttcag ttcagcaag gttcaagtt aaaagtcat 
ggagaatac gaagtttgt agaaactca tttcggaca 
atatccgat aatcgaaat aaattttct ctgttgtga 
tgacgtgac aagcaaatc tgatgcagt gaatgtgtg 
tagaagcgc tatatcgga agcttttag gcaacacag 
ttaccttta cgaatccaa attgtcacc gttgaaaga 
ctatgaaaa aaagcattg cgaacaatg gtgtgacga 
atttaccta cacaggaat tccagatga caaagtaat 
caaggggtt gggatgctt ggcagctta tctgaataa 
gtttcataa aagaatggt ttttctact cgttgacga 
gtacgactc gatcatttc aaaagcgtt gtataaagt 
gaagaacgc tgatccaga gactgagct tgaagagct 
agagaatgc tgcctcatt cccaattga agtaattgg 
atatgctct aaaaaaaat tcctgggat ctttaaagg 
agcgtatat taccggaat tttggatgt gggtggtgt 
agcgatttc caccctatt gggtaatgt attgcgacg 
gcaattcgg aaccaacaa tctgtgcca agatttcta 
tggattcac tgaagatga agtttgtac tctatttaa 
tcggtttgc agtaaagaa ggaattgag agaaaaagc 
gaaggctaa atataatgg gtatttaaa tggctcgaa 
acaatgcct atataatcc tcaaacgat tgtgcagtt 
tttgggaaa tttatctct gggagaagg tgctttaaa 
aaattattg gcaacaatc tggttacat tcacaatat 
gtataaact acttgaaga gggtagcgt gagaaggtt 
gttgtatga acttctaca aggtgttaa agaagattt 
tcaattcac gttaaaacc gttagactt aagtgatct 
gaaatcttt gcataacat attagctaa acaaagttt 
gacttgtcg cgaagaaat agttgttcg atttcttgt 
atctctagg atacttaac atattcatt tgatgatca 
caaactcca aatacctat ccctgagct aattgatga 
atttatgaa gctgctacg agcttattt ttttgaaag 
atttggtat taacagaga attggctga agattgttc 
agcatcatt ttctaagct aaccgttct ctgtggtga 
aggtgattt attagcaaa gttcaaaga agaattagc 
aaattttca aaatagtat aacctgtct acttcaacg 
ttcaacact tatcgatcc attgaatga ggcaacttt 
acagagtat aattacatt gacatgtag taaagcagg 
atttctctt cggaaacga aataacaat tccaattca 
tagaaccaa agttgatat agtactgtt taatgataa 
atctgggat aataatgga attaaaatg tggtccaaa 
tactgtaca agacgcatt taaacaaat ctttcaaaa 
tcggtacta caatgtttt cacaaataa gaagtattt 
tgatgaacg taagaaatc tccttctct ttcttttgt 
tatggcaat gcatgtgga ttctgacaa caaaggatc 
tgtgatgtg cctgcgacg tgaggatgt tattcacgt 
tctggatga tctgcaact tgacgctag agagtcatc 
gctagaagg tcgcattga gatagctgt taattctat 
gggtgaacc tttcattaa cacttgttg cgaaagtgc 
aattcttgt ctcttaaca taccagaga aaccaaaag 
ctgcggttc agtttttgt ttgaattta aagtagcta 
tttttaagt tcaacgcgg catgttaat ttcagtttc 
gatcaacta aatctgttc catttca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY