General information | |||
TC006905 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451351.1: 4063..7264 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC006905 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01100 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06779 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC006905 RA
MEDNPAKRL KSTLSPVFE DVLKSDTFV DKSALMLAV FETDDKPMV LCTAPRRFG KTVNLSMMQ RFLELEVDE NGEEKTKVL KEKDNYKLF SSARFKLKV MENTKFVET HFGQYPIIE INFLCCDDV TSKSDAVNV CRSAISEAF RQHSYLYES KLSPLKDYE KKHCEQWCD EFTYTGIPD DKVIKGLGC LAAYLNKFH KRMVFLLVD EYDSIISKA LYKVKNADP ETELEELEN AASFPIEVI GYALKKIPG IFKGAYITG ILDVGGVAI STLLGNVLR RQFGTNNLC QDFYGFTED EVCTLFNRF AVKKELREK AKAKYNGYL NGSKQCLYN PQTIVQFLG NLSLGEGAL KNYWQQSGY IHNMYKLLE EGSVRRLLY ELLQGVKED FQFTLKPLD LSDLKSLHN ILAKQSLTC REEIVVRFL VSLGYLTYS FDDHKLQIP IPELIDEFM KLLRAYFFE RFGINRELA EDCSASFSK LTVLCGEGD LLAKFKEEL ANFQNSITC LLQRSTLID PLNEATLQS IITLTCSKA GFLFGNEIT IPIHRTKVD IVLFNDKSG IIMELKCGP NTVQDAFKQ IFQNRYYNV FTNKKYFDE RKKSPSLSF VMAMHVDSD NKGSVMCLR REDVIHVLD DLQLDARES SLEGRIEIA VNSMGEPFI NTCCESAIL VS
- >TC006905 RA
atggaggat aatccggcg aaacgttta aaatctacc ctttcaccg gtttttgaa gatgtcttg aaatccgat acttttgtt gataaaagt gcgttgatg ttagcagtt tttgaaacg gacgataaa cctatggta ttgtgtacc gcaccgcgc cgatttggc aaaactgta aacttatcc atgatgcaa cgattcttg gagttggaa gtagatgaa aatggagag gagaaaaca aaggttttg aaagaaaaa gacaattac aaactgttc agttcagca aggttcaag ttaaaagtc atggagaat acgaagttt gtagaaact catttcgga caatatccg ataatcgaa ataaatttt ctctgttgt gatgacgtg acaagcaaa tctgatgca gtgaatgtg tgtagaagc gctatatcg gaagctttt aggcaacac agttacctt tacgaatcc aaattgtca ccgttgaaa gactatgaa aaaaagcat tgcgaacaa tggtgtgac gaatttacc tacacagga attccagat gacaaagta atcaagggg ttgggatgc ttggcagct tatctgaat aagtttcat aaaagaatg gtttttcta ctcgttgac gagtacgac tcgatcatt tcaaaagcg ttgtataaa gtgaagaac gctgatcca gagactgag cttgaagag ctagagaat gctgcctca ttcccaatt gaagtaatt ggatatgct ctaaaaaaa attcctggg atctttaaa ggagcgtat attaccgga attttggat gtgggtggt gtagcgatt tccacccta ttgggtaat gtattgcga cggcaattc ggaaccaac aatctgtgc caagatttc tatggattc actgaagat gaagtttgt actctattt aatcggttt gcagtaaag aaggaattg agagaaaaa gcgaaggct aaatataat gggtattta aatggctcg aaacaatgc ctatataat cctcaaacg attgtgcag tttttggga aatttatct ctgggagaa ggtgcttta aaaaattat tggcaacaa tctggttac attcacaat atgtataaa ctacttgaa gagggtagc gtgagaagg ttgttgtat gaacttcta caaggtgtt aaagaagat tttcaattc acgttaaaa ccgttagac ttaagtgat ctgaaatct ttgcataac atattagct aaacaaagt ttgacttgt cgcgaagaa atagttgtt cgatttctt gtatctcta ggatactta acatattca tttgatgat cacaaactc caaatacct atccctgag ctaattgat gaatttatg aagctgcta cgagcttat ttttttgaa agatttggt attaacaga gaattggct gaagattgt tcagcatca ttttctaag ctaaccgtt ctctgtggt gaaggtgat ttattagca aagttcaaa gaagaatta gcaaatttt caaaatagt ataacctgt ctacttcaa cgttcaaca cttatcgat ccattgaat gaggcaact ttacagagt ataattaca ttgacatgt agtaaagca ggatttctc ttcggaaac gaaataaca attccaatt catagaacc aaagttgat atagtactg tttaatgat aaatctggg ataataatg gaattaaaa tgtggtcca aatactgta caagacgca tttaaacaa atctttcaa aatcggtac tacaatgtt ttcacaaat aagaagtat tttgatgaa cgtaagaaa tctccttct ctttctttt gttatggca atgcatgtg gattctgac aacaaagga tctgtgatg tgcctgcga cgtgaggat gttattcac gttctggat gatctgcaa cttgacgct agagagtca tcgctagaa ggtcgcatt gagatagct gttaattct atgggtgaa cctttcatt aacacttgt tgcgaaagt gcaattctt gtctcttaa
- >TC006905 RA
tgtttattt ttttctgtt ccgtataaa agatttaaa actgttttt gaattgttg gtgtgcttg ttaacagtg tgtagttaa tactatggg tcaatcttg cttgcttta ttttttaca atttagtaa tgaaaaaca gtatttttc gatttattg tgcaaacat taattcgtg gttttcatt cattgttac aggcagttt gtttattat ggaggataa tccggcgaa acgtttaaa atctaccct ttcaccggt ttttgaaga tgtcttgaa atccgatac ttttgttga taaaagtgc gttgatgtt agcagtttt tgaaacgga cgataaacc tatggtatt gtgtaccgc accgcgccg atttggcaa aactgtaaa cttatccat gatgcaacg attcttgga gttggaagt agatgaaaa tggagagga gaaaacaaa ggttttgaa agaaaaaga caattacaa actgttcag ttcagcaag gttcaagtt aaaagtcat ggagaatac gaagtttgt agaaactca tttcggaca atatccgat aatcgaaat aaattttct ctgttgtga tgacgtgac aagcaaatc tgatgcagt gaatgtgtg tagaagcgc tatatcgga agcttttag gcaacacag ttaccttta cgaatccaa attgtcacc gttgaaaga ctatgaaaa aaagcattg cgaacaatg gtgtgacga atttaccta cacaggaat tccagatga caaagtaat caaggggtt gggatgctt ggcagctta tctgaataa gtttcataa aagaatggt ttttctact cgttgacga gtacgactc gatcatttc aaaagcgtt gtataaagt gaagaacgc tgatccaga gactgagct tgaagagct agagaatgc tgcctcatt cccaattga agtaattgg atatgctct aaaaaaaat tcctgggat ctttaaagg agcgtatat taccggaat tttggatgt gggtggtgt agcgatttc caccctatt gggtaatgt attgcgacg gcaattcgg aaccaacaa tctgtgcca agatttcta tggattcac tgaagatga agtttgtac tctatttaa tcggtttgc agtaaagaa ggaattgag agaaaaagc gaaggctaa atataatgg gtatttaaa tggctcgaa acaatgcct atataatcc tcaaacgat tgtgcagtt tttgggaaa tttatctct gggagaagg tgctttaaa aaattattg gcaacaatc tggttacat tcacaatat gtataaact acttgaaga gggtagcgt gagaaggtt gttgtatga acttctaca aggtgttaa agaagattt tcaattcac gttaaaacc gttagactt aagtgatct gaaatcttt gcataacat attagctaa acaaagttt gacttgtcg cgaagaaat agttgttcg atttcttgt atctctagg atacttaac atattcatt tgatgatca caaactcca aatacctat ccctgagct aattgatga atttatgaa gctgctacg agcttattt ttttgaaag atttggtat taacagaga attggctga agattgttc agcatcatt ttctaagct aaccgttct ctgtggtga aggtgattt attagcaaa gttcaaaga agaattagc aaattttca aaatagtat aacctgtct acttcaacg ttcaacact tatcgatcc attgaatga ggcaacttt acagagtat aattacatt gacatgtag taaagcagg atttctctt cggaaacga aataacaat tccaattca tagaaccaa agttgatat agtactgtt taatgataa atctgggat aataatgga attaaaatg tggtccaaa tactgtaca agacgcatt taaacaaat ctttcaaaa tcggtacta caatgtttt cacaaataa gaagtattt tgatgaacg taagaaatc tccttctct ttcttttgt tatggcaat gcatgtgga ttctgacaa caaaggatc tgtgatgtg cctgcgacg tgaggatgt tattcacgt tctggatga tctgcaact tgacgctag agagtcatc gctagaagg tcgcattga gatagctgt taattctat gggtgaacc tttcattaa cacttgttg cgaaagtgc aattcttgt ctcttaaca taccagaga aaccaaaag ctgcggttc agtttttgt ttgaattta aagtagcta tttttaagt tcaacgcgg catgttaat ttcagtttc gatcaacta aatctgttc catttca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
