Status
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Gene information
General information
TC006905 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451351.1: 4063..7264
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006905
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01100

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      Fertility
      egg chamber mostly not present - Number of animals showing the phenotype: 4

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection


      pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
      Days post injection


      iBeetle screen iB_06779

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      Adult morphology
      prothoracic odoriferous glands darker - Number of animals showing the phenotype: 2 (13 dpi)
      Days post injection



      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006905 RA
        MEDNPAKRL KSTLSPVFE DVLKSDTFV DKSALMLAV 
        FETDDKPMV LCTAPRRFG KTVNLSMMQ RFLELEVDE 
        NGEEKTKVL KEKDNYKLF SSARFKLKV MENTKFVET 
        HFGQYPIIE INFLCCDDV TSKSDAVNV CRSAISEAF 
        RQHSYLYES KLSPLKDYE KKHCEQWCD EFTYTGIPD 
        DKVIKGLGC LAAYLNKFH KRMVFLLVD EYDSIISKA 
        LYKVKNADP ETELEELEN AASFPIEVI GYALKKIPG 
        IFKGAYITG ILDVGGVAI STLLGNVLR RQFGTNNLC 
        QDFYGFTED EVCTLFNRF AVKKELREK AKAKYNGYL 
        NGSKQCLYN PQTIVQFLG NLSLGEGAL KNYWQQSGY 
        IHNMYKLLE EGSVRRLLY ELLQGVKED FQFTLKPLD 
        LSDLKSLHN ILAKQSLTC REEIVVRFL VSLGYLTYS 
        FDDHKLQIP IPELIDEFM KLLRAYFFE RFGINRELA 
        EDCSASFSK LTVLCGEGD LLAKFKEEL ANFQNSITC 
        LLQRSTLID PLNEATLQS IITLTCSKA GFLFGNEIT 
        IPIHRTKVD IVLFNDKSG IIMELKCGP NTVQDAFKQ 
        IFQNRYYNV FTNKKYFDE RKKSPSLSF VMAMHVDSD 
        NKGSVMCLR REDVIHVLD DLQLDARES SLEGRIEIA 
        VNSMGEPFI NTCCESAIL VS
      CDS sequence
      • >TC006905 RA
        atggaggat aatccggcg aaacgttta aaatctacc 
        ctttcaccg gtttttgaa gatgtcttg aaatccgat 
        acttttgtt gataaaagt gcgttgatg ttagcagtt 
        tttgaaacg gacgataaa cctatggta ttgtgtacc 
        gcaccgcgc cgatttggc aaaactgta aacttatcc 
        atgatgcaa cgattcttg gagttggaa gtagatgaa 
        aatggagag gagaaaaca aaggttttg aaagaaaaa 
        gacaattac aaactgttc agttcagca aggttcaag 
        ttaaaagtc atggagaat acgaagttt gtagaaact 
        catttcgga caatatccg ataatcgaa ataaatttt 
        ctctgttgt gatgacgtg acaagcaaa tctgatgca 
        gtgaatgtg tgtagaagc gctatatcg gaagctttt 
        aggcaacac agttacctt tacgaatcc aaattgtca 
        ccgttgaaa gactatgaa aaaaagcat tgcgaacaa 
        tggtgtgac gaatttacc tacacagga attccagat 
        gacaaagta atcaagggg ttgggatgc ttggcagct 
        tatctgaat aagtttcat aaaagaatg gtttttcta 
        ctcgttgac gagtacgac tcgatcatt tcaaaagcg 
        ttgtataaa gtgaagaac gctgatcca gagactgag 
        cttgaagag ctagagaat gctgcctca ttcccaatt 
        gaagtaatt ggatatgct ctaaaaaaa attcctggg 
        atctttaaa ggagcgtat attaccgga attttggat 
        gtgggtggt gtagcgatt tccacccta ttgggtaat 
        gtattgcga cggcaattc ggaaccaac aatctgtgc 
        caagatttc tatggattc actgaagat gaagtttgt 
        actctattt aatcggttt gcagtaaag aaggaattg 
        agagaaaaa gcgaaggct aaatataat gggtattta 
        aatggctcg aaacaatgc ctatataat cctcaaacg 
        attgtgcag tttttggga aatttatct ctgggagaa 
        ggtgcttta aaaaattat tggcaacaa tctggttac 
        attcacaat atgtataaa ctacttgaa gagggtagc 
        gtgagaagg ttgttgtat gaacttcta caaggtgtt 
        aaagaagat tttcaattc acgttaaaa ccgttagac 
        ttaagtgat ctgaaatct ttgcataac atattagct 
        aaacaaagt ttgacttgt cgcgaagaa atagttgtt 
        cgatttctt gtatctcta ggatactta acatattca 
        tttgatgat cacaaactc caaatacct atccctgag 
        ctaattgat gaatttatg aagctgcta cgagcttat 
        ttttttgaa agatttggt attaacaga gaattggct 
        gaagattgt tcagcatca ttttctaag ctaaccgtt 
        ctctgtggt gaaggtgat ttattagca aagttcaaa 
        gaagaatta gcaaatttt caaaatagt ataacctgt 
        ctacttcaa cgttcaaca cttatcgat ccattgaat 
        gaggcaact ttacagagt ataattaca ttgacatgt 
        agtaaagca ggatttctc ttcggaaac gaaataaca 
        attccaatt catagaacc aaagttgat atagtactg 
        tttaatgat aaatctggg ataataatg gaattaaaa 
        tgtggtcca aatactgta caagacgca tttaaacaa 
        atctttcaa aatcggtac tacaatgtt ttcacaaat 
        aagaagtat tttgatgaa cgtaagaaa tctccttct 
        ctttctttt gttatggca atgcatgtg gattctgac 
        aacaaagga tctgtgatg tgcctgcga cgtgaggat 
        gttattcac gttctggat gatctgcaa cttgacgct 
        agagagtca tcgctagaa ggtcgcatt gagatagct 
        gttaattct atgggtgaa cctttcatt aacacttgt 
        tgcgaaagt gcaattctt gtctcttaa
      mRNA sequence
      • >TC006905 RA
        tgtttattt ttttctgtt ccgtataaa agatttaaa 
        actgttttt gaattgttg gtgtgcttg ttaacagtg 
        tgtagttaa tactatggg tcaatcttg cttgcttta 
        ttttttaca atttagtaa tgaaaaaca gtatttttc 
        gatttattg tgcaaacat taattcgtg gttttcatt 
        cattgttac aggcagttt gtttattat ggaggataa 
        tccggcgaa acgtttaaa atctaccct ttcaccggt 
        ttttgaaga tgtcttgaa atccgatac ttttgttga 
        taaaagtgc gttgatgtt agcagtttt tgaaacgga 
        cgataaacc tatggtatt gtgtaccgc accgcgccg 
        atttggcaa aactgtaaa cttatccat gatgcaacg 
        attcttgga gttggaagt agatgaaaa tggagagga 
        gaaaacaaa ggttttgaa agaaaaaga caattacaa 
        actgttcag ttcagcaag gttcaagtt aaaagtcat 
        ggagaatac gaagtttgt agaaactca tttcggaca 
        atatccgat aatcgaaat aaattttct ctgttgtga 
        tgacgtgac aagcaaatc tgatgcagt gaatgtgtg 
        tagaagcgc tatatcgga agcttttag gcaacacag 
        ttaccttta cgaatccaa attgtcacc gttgaaaga 
        ctatgaaaa aaagcattg cgaacaatg gtgtgacga 
        atttaccta cacaggaat tccagatga caaagtaat 
        caaggggtt gggatgctt ggcagctta tctgaataa 
        gtttcataa aagaatggt ttttctact cgttgacga 
        gtacgactc gatcatttc aaaagcgtt gtataaagt 
        gaagaacgc tgatccaga gactgagct tgaagagct 
        agagaatgc tgcctcatt cccaattga agtaattgg 
        atatgctct aaaaaaaat tcctgggat ctttaaagg 
        agcgtatat taccggaat tttggatgt gggtggtgt 
        agcgatttc caccctatt gggtaatgt attgcgacg 
        gcaattcgg aaccaacaa tctgtgcca agatttcta 
        tggattcac tgaagatga agtttgtac tctatttaa 
        tcggtttgc agtaaagaa ggaattgag agaaaaagc 
        gaaggctaa atataatgg gtatttaaa tggctcgaa 
        acaatgcct atataatcc tcaaacgat tgtgcagtt 
        tttgggaaa tttatctct gggagaagg tgctttaaa 
        aaattattg gcaacaatc tggttacat tcacaatat 
        gtataaact acttgaaga gggtagcgt gagaaggtt 
        gttgtatga acttctaca aggtgttaa agaagattt 
        tcaattcac gttaaaacc gttagactt aagtgatct 
        gaaatcttt gcataacat attagctaa acaaagttt 
        gacttgtcg cgaagaaat agttgttcg atttcttgt 
        atctctagg atacttaac atattcatt tgatgatca 
        caaactcca aatacctat ccctgagct aattgatga 
        atttatgaa gctgctacg agcttattt ttttgaaag 
        atttggtat taacagaga attggctga agattgttc 
        agcatcatt ttctaagct aaccgttct ctgtggtga 
        aggtgattt attagcaaa gttcaaaga agaattagc 
        aaattttca aaatagtat aacctgtct acttcaacg 
        ttcaacact tatcgatcc attgaatga ggcaacttt 
        acagagtat aattacatt gacatgtag taaagcagg 
        atttctctt cggaaacga aataacaat tccaattca 
        tagaaccaa agttgatat agtactgtt taatgataa 
        atctgggat aataatgga attaaaatg tggtccaaa 
        tactgtaca agacgcatt taaacaaat ctttcaaaa 
        tcggtacta caatgtttt cacaaataa gaagtattt 
        tgatgaacg taagaaatc tccttctct ttcttttgt 
        tatggcaat gcatgtgga ttctgacaa caaaggatc 
        tgtgatgtg cctgcgacg tgaggatgt tattcacgt 
        tctggatga tctgcaact tgacgctag agagtcatc 
        gctagaagg tcgcattga gatagctgt taattctat 
        gggtgaacc tttcattaa cacttgttg cgaaagtgc 
        aattcttgt ctcttaaca taccagaga aaccaaaag 
        ctgcggttc agtttttgt ttgaattta aagtagcta 
        tttttaagt tcaacgcgg catgttaat ttcagtttc 
        gatcaacta aatctgttc catttca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY