Status
Please wait ...
Gene information
General information
TC006901 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450753.1: 1655..3635
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006901
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01099

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    mature egg deposition blocked number 1st lateral oviduct: 3 - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006901 RA
      MDFGTLLHT AQKNEKGSK KEPVRYYST KFEPPKKEQ 
      RSKQLSENI KKFLAKKEA EEREKKLEA LKKKEELLA 
      LRAKDKKAT RRVNVMLKR TKSANQSVL QDAVDANNT 
      SVTLAGPIQ PDEDDYGYV SQEAAAFYN KMMEKYSKM 
      PDEPKFGLN KKKKISTDL NSTKERVKA AIEREREEA 
      NMPHKRRRK HKEEEEEIE NEKQEVAPK PKPKPAAPP 
      PLNFNDLLK LAEKKQFEP IIIEQKPKE EEKLFTKKQ 
      KKELEREKE WRERKEGKL PPLEKPAGN KIPKVNNGD 
      KAQALAKKN STDLIKKSQ VKVPEPTKK IVSNNQPIV 
      DKKPVPSYT KPGSSSLNK VKDLNDVRK REAPKSGGR 
      DVVAPKKPQ LELKPKQFP PKDLKPKQF PPPDVRRKE 
      FPPRDVKRK PLVNKRRIL DDDEYDSEM DDFIDDGPE 
      EGEDYSKYI SEIFGYDKS KYRNVDDDI DNMESTFAQ 
      QMREEVIST KIGIMEDLE DMKKEEEEK RRKALMKKK 
      RL
    CDS sequence
    • >TC006901 RA
      atggatttt ggtacgctt ttgcacacc gcccaaaaa 
      aatgagaaa ggctccaag aaagagccc gtcaggtac 
      tacagtacc aaattcgag cctccaaag aaggaacaa 
      cgcagtaaa caattgtcg gaaaacatc aaaaagttt 
      ttagcgaag aaggaggcc gaagagagg gaaaagaaa 
      ctcgaagct ttgaagaaa aaggaggaa ttgttggct 
      ctgagggct aaggacaaa aaagcaaca agacgggtt 
      aatgtgatg ttaaagcgt actaaatct gcaaaccaa 
      tccgtgctt caagacgct gttgatgct aacaacact 
      tcggtgact ttagccggc cccattcag ccagacgag 
      gatgactat ggctatgtc tcccaggaa gcggcagcg 
      ttttacaat aaaatgatg gaaaagtac agcaaaatg 
      ccagacgag cccaaattt ggcttaaac aagaagaag 
      aaaataagc acggattta aacagcact aaggagcgg 
      gtcaaggct gctattgag agagaacgg gaggaggca 
      aacatgccc cacaaaagg cgaagaaag cacaaagag 
      gaagaggaa gaaattgag aatgagaag caggaagtg 
      gccccaaag cctaaaccg aaacctgca gcgccgccc 
      cctttaaac ttcaatgac ttgctgaaa ctggccgag 
      aagaagcaa ttcgaaccc ataataatt gaacaaaag 
      ccaaaggaa gaggagaaa ttgtttacg aagaagcag 
      aaaaaggag ctggagagg gagaaggaa tggagggag 
      cggaaggag ggaaaactt ccgcctttg gagaaacca 
      gctgggaac aaaatcccc aaagtgaac aacggggat 
      aaagcgcaa gctttggca aagaaaaat tcaacagat 
      ctgattaag aaaagtcaa gttaaggtg ccagaaccc 
      acaaagaaa attgtgtca aataaccag cccattgtt 
      gataagaaa cctgtgcct tcctacacg aaaccgggg 
      tcaagtagt ttaaacaaa gtcaaagat ttgaatgat 
      gtgagaaag agagaagcg ccaaaatcg ggtgggaga 
      gacgttgtt gcacctaaa aaacctcaa cttgagctt 
      aaacctaaa caatttccc ccgaaagat cttaaaccg 
      aagcagttc ccgcctcct gatgtcagg agaaaagag 
      tttccgcct cgtgatgtt aagagaaaa ccgcttgtc 
      aataaaagg cgtattttg gatgatgat gagtacgat 
      tctgaaatg gatgacttt attgatgac gggccggaa 
      gaaggcgaa gattactct aaatacatt agcgagatt 
      tttggatat gataaatcg aaatataga aatgttgac 
      gatgatatt gataatatg gaatcaacg tttgcacag 
      caaatgagg gaagaagtg atcagtaca aaaattggt 
      atcatggag gatttagaa gatatgaag aaagaggaa 
      gaagagaaa aggcggaaa gccttgatg aaaaaaaag 
      agattataa
    mRNA sequence
    • >TC006901 RA
      ataatggcg catgatgac atgtacgac gtaaaagtg 
      aaatatgtc atgacataa atcggattt ttctcagtt 
      tttttgagc aattagcac ggaaaaaca tggattttg 
      gtacgcttt tgcacaccg cccaaaaaa atgagaaag 
      gctccaaga aagagcccg tcaggtact acagtacca 
      aattcgagc ctccaaaga aggaacaac gcagtaaac 
      aattgtcgg aaaacatca aaaagtttt tagcgaaga 
      aggaggccg aagagaggg aaaagaaac tcgaagctt 
      tgaagaaaa aggaggaat tgttggctc tgagggcta 
      aggacaaaa aagcaacaa gacgggtta atgtgatgt 
      taaagcgta ctaaatctg caaaccaat ccgtgcttc 
      aagacgctg ttgatgcta acaacactt cggtgactt 
      tagccggcc ccattcagc cagacgagg atgactatg 
      gctatgtct cccaggaag cggcagcgt tttacaata 
      aaatgatgg aaaagtaca gcaaaatgc cagacgagc 
      ccaaatttg gcttaaaca agaagaaga aaataagca 
      cggatttaa acagcacta aggagcggg tcaaggctg 
      ctattgaga gagaacggg aggaggcaa acatgcccc 
      acaaaaggc gaagaaagc acaaagagg aagaggaag 
      aaattgaga atgagaagc aggaagtgg ccccaaagc 
      ctaaaccga aacctgcag cgccgcccc ctttaaact 
      tcaatgact tgctgaaac tggccgaga agaagcaat 
      tcgaaccca taataattg aacaaaagc caaaggaag 
      aggagaaat tgtttacga agaagcaga aaaaggagc 
      tggagaggg agaaggaat ggagggagc ggaaggagg 
      gaaaacttc cgcctttgg agaaaccag ctgggaaca 
      aaatcccca aagtgaaca acggggata aagcgcaag 
      ctttggcaa agaaaaatt caacagatc tgattaaga 
      aaagtcaag ttaaggtgc cagaaccca caaagaaaa 
      ttgtgtcaa ataaccagc ccattgttg ataagaaac 
      ctgtgcctt cctacacga aaccggggt caagtagtt 
      taaacaaag tcaaagatt tgaatgatg tgagaaaga 
      gagaagcgc caaaatcgg gtgggagag acgttgttg 
      cacctaaaa aacctcaac ttgagctta aacctaaac 
      aatttcccc cgaaagatc ttaaaccga agcagttcc 
      cgcctcctg atgtcagga gaaaagagt ttccgcctc 
      gtgatgtta agagaaaac cgcttgtca ataaaaggc 
      gtattttgg atgatgatg agtacgatt ctgaaatgg 
      atgacttta ttgatgacg ggccggaag aaggcgaag 
      attactcta aatacatta gcgagattt ttggatatg 
      ataaatcga aatatagaa atgttgacg atgatattg 
      ataatatgg aatcaacgt ttgcacagc aaatgaggg 
      aagaagtga tcagtacaa aaattggta tcatggagg 
      atttagaag atatgaaga aagaggaag aagagaaaa 
      ggcggaaag ccttgatga aaaaaaaga gattataat 
      tgtagctgt tgtgattgt ggcatgact ttgtgatag 
      gtgattgat aacttttag gaacacttg aaattgtaa 
      tttttattg ttatccttt tattacgga aaatttaaa 
      ctttaatat atactagac cgccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY