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Gene information
General information
TC006853 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 48378..68165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006853
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01098

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 2
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    dorsal ridge row mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet seta partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    vertex triplet vertex triplet ventral not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labium position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs pretarsus slightly shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdominal segment 1 cuticle ectopically with protrusion - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 5

    wings not closed - Number of animals showing the phenotype: 5

    wings potentially shortened - Number of animals showing the phenotype: 5
    iBeetle screen iB_04227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva bristle potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bristle potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006853 RA
      MSQRAKRPN RSDDGSVPV KRRKRTAAE EEEAASLAV 
WQPRSNDLK SIYNRTTTE APAELFRKD LISAMKLPD 
SEPLASNEY WVIVDQWKQ EWERGVQVP VNPDSLPEP 
SVTVTQNTP LKLQHEFKL PKNKYIRIT KDENFKIEE 
HALSNAPAQ SEAACSYDL DECDVSWLR ILNSERSQC 
GQSPINSEQ LERVIEQFE LCCWDKIQS ILRNEEGLG 
IEYDENVIC DVCRSPDSE EGNEMVFCD SCNICVHQA 
CYGITRIPE GQWLCCTCH LSKRPKCVL CPNKGGAMK 
CTRSGQKWA HVSCALWIP EVSIGCVEK MEPITKISS 
IPQSRWALI CVLCRERVG ACIQCSVKT CKTAYHVTC 
AFKHGLEMR AIIEDENAD DGVKLRSYC EKHSKSSKN 
KEKSVCSGS EDDDSRRKK RKDMTSEEK NQARAARLQ 
EIESEFFKH VSIKDVSIH LDVDNDALH HIYNYWKLK 
RKAGNNKPL LPPKSEDVD MLSHKREQA DMEKMKMFV 
QLRQDLERV RNLCYMVSR REKLSRSFF RLREQTFYK 
QTAVLEAAN NLPNVVVQA VIEANHGPS IYDRLYSHE 
DAEDHTSDF ERILTRIAG VKSPTDSGD EKRTELNGL 
FKDKNPYKK IYFNGSSNR RSGSLYGSS SSSDEKDRL 
NAATTSEDE DKSKKKSSS SVRKPNNNN NKASDRRRK 
RHSASSKIE SSSEEDSKQ KTKKTWGQF SRLKQVERE 
LGYSGSDSD ELMPIRASQ KSDTKQMSS IYSDSDSSD 
VSMTKASDS QQNKLRTKA AVKEFSHKP STSNKTSPE 
NKKNKKKES PTKKKDYDP SDLIVPQRQ AAKKATENL 
KSTTTRVVK EQMDEPSVK VEEKPAKLP KPKAKTKET 
VKEVKEVKK ESAVKTSDV FDLDKETDN AEILAYVPQ 
RQAAKKAAE HIKSGLGKP VAPSSVDEL EKNKLESTV 
VKKKEAEKQ QPAKVPESK RKSSSNSSS SSSSSSSTS 
SSTSSSSDS DDEVPPSQQ QAQQPRSES LFSNYSSKE 
CRETAKRTN ASKDWPFLD KGAKSAAAA ASSSSTDSS 
DSSRPTSPI ITTTTTTKT TSPDKKSTT TQREEQTRM 
RGKGRPATT AARGRPRQS SKSGDRVGD ANVRLRNLS 
DSQRGDAKC RKGKQDEVA EKETKSVNK LQSPVRKTD 
KKLTESKES SEVSNLEKE ILERKAGKQ GSSKTKSTL 
DRLFGSPPD KKDEKKPLN KTTKPTKPE RVKSTVKEN 
KEPEKIASP VKKSPVKSK NIDEIFTQK TVLNENKIE 
VKEKEKHPT PKREEIEPA PKIEDFKQN NEKLTEPEK 
KLPHSRSIF SPPHLKDNL LDFDNLDDG FGISKDEEI 
MKGPLTFNF GNTSLFKED SKEDSARET LNLVEKLRM 
EMSKKSTSC DVDDGASVS SSTKNECDR TEFAEQVVP 
NCVAEKNDA KVDDVPPPT VNLEMRVEV TEAKGFADR 
GQDYRFMSC EADVPLEQN KNIPIQQHH GLSDHGISG 
QGDERWVPP SEAYTAAPP IDPSNYGSE SQQPLQTPQ 
HFMQQYSLN KTQPLLNAI PPQIEMRLH DDTLKNPSP 
TLHDRRISQ TPLNEDCMP SPSPYADIH PQAKWADSE 
IMPNRRSSS SSAASTSSS CSRRNDDDD LKLRPDVMM 
VNHQALEIG YLHPFPPEL FPTLSDSSQ FVSPVSLLH 
PPPLNPSLA FPSTAPAML PPAFAPFPP AQVMAPTLS 
KPLEDVPHC TAAFTSSQH NMALTAAIA NLPPSLPKE 
VEALPEDEV VPTTQSNVL SSPSPSIKS NSSGKKSPS 
KPTRTSARV TSQGKSPKQ DVVKPSGRG RGEAKRNSV 
KSNAARGRG RARGRGRAH SHNDHEFIS NTIHNKLVG 
TVYDLDFDD DISSENVDL KSMRERRKS VDVQKTELK 
ESSDSPSQK KYNDLRELR PPSPVKDTT PKPAEVEPI 
FPDIVQPVL PGPVDMRTY NTNFDQSYN EANLLRAFD 
SGTTEPPVN EEIDEEFEK ELQTALTTP SKKPDEPPA 
NIKVSLSDS RNQLKVKIK GPIANYSST IPPLPPPVT 
VSSISNNVI SNNSIGVSS GTSNLRRMR KKELLRQYW 
TQDMNMDDS SCAPGFNAT PTVPPVNRT IITIPKAVA 
SMTSIPTKD DYRDYRTDD VIETKQHRK EAKSRPGAL 
SRELRQLDL SLDEDALHE RRRSIGSNN STNYDSGTN 
KRRGRPPRP SQQTTGTPK IKIKIGNSI VGKIDDKRD 
RIRPPKKRL ANINMPSVE DLKRESMKF RKLVMADFD 
EGKEKRKKK DKSEKRKRK KAKPEVRII SNESQNKLI 
IRIGKKGER TSGGEEGGA PIKLKLARC QEGSGYVMK 
SEEAAQSPP PPPPPPPPT LGPPPLPLN KDCEVR
    CDS sequence
    • >TC006853 RA
      atgtcacag agagcaaaa aggcctaac cgctcagac 
gatggatca gttcctgtt aaaaggcgc aaacgtacg 
gcggcggag gaggaggaa gcggcctcc ttggcggtg 
tggcagccc cgctccaac gacctgaag agcatctac 
aaccggacg acgaccgag gcgccggcc gagctcttc 
cgcaaagac ctgatcagt gcgatgaag ttgcccgat 
tcggagccg ctggcatcg aatgagtac tgggttatt 
gtcgatcag tggaagcag gagtgggaa aggggggtc 
caggtgcca gtcaatcca gattccttg ccggaacct 
agcgtcact gtcacacag aacacgcca ctcaagctg 
caacatgag ttcaaactg ccaaaaaac aagtacatc 
cgcatcacc aaggacgag aacttcaaa atcgaggag 
cacgcccta tccaacgcc ccggctcaa tcggaggcg 
gcttgctcg tacgacctg gacgaatgc gacgtctcc 
tggctgcgc atcctcaac tcggagcgc tcccaatgc 
ggccaatca ccgatcaac tccgagcaa ctcgagcgc 
gtaatcgag caattcgag ctatgctgc tgggacaaa 
atccagtcg attttacgc aacgaagaa ggcctgggc 
atcgaatac gacgagaac gtcatctgc gacgtctgt 
cgctccccc gactccgaa gaaggcaac gagatggtc 
ttctgcgac agctgcaac atctgcgtc caccaggcc 
tgctacggc atcacccgg atacccgaa ggccaatgg 
ctgtgctgc acgtgccac ctcagcaag cggccgaaa 
tgcgtgctc tgccccaac aaagggggc gccatgaag 
tgcacgcgc tccggccag aagtgggcg cacgtctcg 
tgcgccctc tggataccc gaagtgagc atcggctgt 
gtggagaaa atggaaccc ataacgaaa atctcaagc 
attccgcag agccgatgg gcgctgatc tgtgtcctg 
tgccgggag agggtcggc gcctgcatc cagtgctcg 
gtcaagacg tgcaaaacg gcgtatcat gtcacctgt 
gcgttcaaa cacggcttg gaaatgcga gcgattatc 
gaagacgag aacgccgat gacggagtt aagttgcgc 
tcatattgc gagaaacac agcaagtcg agcaagaac 
aaggagaag agtgtgtgt tcggggtcg gaggacgac 
gattcgcgc cggaaaaag cgcaaagac atgacttcg 
gaggagaag aaccaggca cgtgctgca cggttacag 
gagatcgag tcggagttt ttcaagcac gtgtcgatt 
aaagacgtc agtatccac ctggacgtg gacaacgac 
gccctccac cacatctac aactactgg aagttgaag 
cgcaaagcc gggaacaac aagccgctg ctgccgccg 
aagtccgag gacgttgat atgctctct cataaacgc 
gaacaggcc gatatggag aagatgaag atgtttgtg 
caattgaga caggacttg gaacgggtg cggaatttg 
tgttatatg gtgagtcgg agagagaaa ctgtcgagg 
agtttcttt cgattgcgc gaacagacg ttttataaa 
cagacggct gtgcttgaa gctgctaac aatttgccg 
aatgttgtg gtacaagct gtgattgaa gccaatcat 
ggaccgtcg atctatgat cggttgtat tcgcatgaa 
gatgcagag gatcatacg agcgatttt gagaggatt 
ttgacgagg attgctggg gttaagtcg ccgacggat 
tcaggggat gagaaacgg acagagctt aatgggctt 
tttaaggac aagaatcca tacaaaaag atttacttc 
aatggttct tcgaatcga cgcagcggc agtttgtac 
ggcagtagt tcgagtagt gacgaaaag gaccgcttg 
aacgcggca acaacttcc gaagatgaa gacaaaagt 
aaaaagaaa tcatcttca tcggtccga aaacctaac 
aacaataac aacaaagcg tccgatcgc cgacgaaaa 
agacacagt gcatctagt aaaatcgaa agtagtagt 
gaggaggat tccaaacag aaaacgaaa aagacttgg 
ggacaattc tcccgctta aagcaagtg gaacgcgaa 
ttaggttat tcgggtagt gacagtgac gaactaatg 
ccaatacgt gcaagccaa aaatcagac actaaacaa 
atgtcgtca atctattcg gatagtgac agttcggac 
gtatcaatg accaaagcg agtgattcc caacaaaat 
aaactacga acgaaagca gcagtcaaa gagttttca 
cacaaaccg tcaacgtcc aacaaaaca agtcccgaa 
aacaaaaag aacaagaaa aaagaaagc cccacaaag 
aaaaaagat tacgaccca tctgatctc attgtaccg 
cagcgacaa gcggccaaa aaagcaacc gaaaatctc 
aaatcgaca acaacgcga gttgtcaaa gaacaaatg 
gacgaacca agcgtaaaa gttgaagaa aaacccgcc 
aaattgccc aaacctaaa gcaaaaact aaagaaacc 
gtaaaggaa gtgaaagaa gtgaagaag gaatcagcg 
gtgaaaacg agcgatgtg ttcgatctc gataaagaa 
acggacaat gcggagatt ctcgcctac gttccgcaa 
cgtcaagct gcgaaaaaa gccgctgaa cacataaaa 
agtgggctg ggtaagccc gtggcgccc tctagtgtt 
gacgaattg gagaaaaac aaattggag agtacagtg 
gtcaagaaa aaggaggcg gaaaaacaa cagccggct 
aaagtccca gaaagcaaa cgcaagtct tcctcaaac 
agcagttcg tcttcctcg tctagttct tccacgtcg 
tcttcgacg tcgtcgagt tcagatagt gatgatgag 
gttccacca agtcagcaa caagcgcaa caaccgcga 
tcggagagt cttttctcg aattactcg tcgaaagag 
tgtcgggaa acggctaag cggacgaac gccagcaag 
gactggccc tttcttgac aagggtgcc aagtctgca 
gcagcagcg gcgtccagc tcaagcacc gactcgagt 
gattctagc cgtcccacc tccccaatc atcacaaca 
acaacaaca acaaaaact actagtccc gacaagaag 
tcaacaact actcaaaga gaggaacag acgagaatg 
cgaggaaaa ggcagacca gcaacaaca gcagctaga 
gggaggccg aggcaatca tctaaatcc ggtgatcgg 
gtcggagat gctaatgtc aggcttaga aatttgtct 
gatagccaa cgtggcgat gctaagtgt cggaaggga 
aaacaggat gaggtggcc gagaaagag acaaagtcc 
gttaacaaa ctacagtct ccggtccgg aaaacggac 
aaaaaacta accgaaagc aaagaatcg tcggaagtc 
agtaatctc gaaaaggaa atactcgaa cgaaaagct 
ggaaaacag ggctcatcc aaaacgaaa tcaactctc 
gaccggtta ttcggttca ccacctgac aaaaaggac 
gaaaagaaa ccgttaaac aaaacgaca aaaccgacc 
aaacctgaa cgagtaaaa tcaacagta aaggaaaac 
aaagagccg gaaaaaata gcatcaccc gtcaaaaaa 
tcgccggtt aaaagcaaa aacatcgat gagattttc 
acacaaaag actgtccta aacgagaat aaaatcgag 
gttaaagaa aaggaaaaa cacccaact ccgaagcgt 
gaagaaatc gaaccggcg ccaaaaatc gaagatttc 
aaacaaaac aacgagaaa ctcaccgaa ccggagaag 
aaattgccg cacagtcgg tcaattttc tcgccaccg 
catctcaaa gacaatttg cttgatttc gataatttg 
gacgacggt tttggtatt tccaaagac gaggagata 
atgaaaggc cccctgacg ttcaatttc ggcaacact 
agtcttttt aaggaagac tcaaaggaa gacagtgca 
cgtgaaacg ctaaatctt gtcgaaaaa cttcgcatg 
gaaatgtcg aaaaaatcg accagttgt gatgttgac 
gatggtgca agtgtttcg tcgagtact aaaaacgaa 
tgtgatcgg actgagttt gccgaacaa gtggtgccg 
aattgtgtg gcggaaaag aacgatgcg aaagtggat 
gatgtgccg ccgccgact gtgaacttg gagatgcgt 
gtggaggtg acggaagcg aaaggtttc gccgataga 
gggcaggac tatcggttt atgagttgt gaggcggat 
gttccattg gaacagaac aagaacatt ccgattcaa 
cagcatcat gggcttagt gatcatggg attagtggg 
cagggtgat gagagatgg gtgccaccc agcgaggcg 
tatacggca gcgccacct atagaccct tcgaattac 
ggcagcgag agccaacaa cctcttcaa acccctcaa 
cacttcatg cagcaatac tcactcaac aagactcaa 
cccctcctc aacgccatt cctccccag atcgaaatg 
cgcctccac gacgacacc ctcaaaaac ccatccccc 
acgttacac gaccgtcga atctcacaa acccccctc 
aacgaagac tgcatgccc agcccatcg ccgtacgcc 
gacatccac ccccaggcc aaatgggcc gacagcgaa 
atcatgccc aaccgacgt tcttcctcc agctcggcg 
gcctccacc tccagctcg tgttcgcgg cgaaacgac 
gacgacgac ctcaaactg cggcccgac gtcatgatg 
gtcaaccac caggctctc gaaatcggc tacctgcac 
ccgtttcca cccgaattg ttcccgact ttgtccgat 
tcctcgcaa tttgtcagt ccggtaagt ctcctccat 
cctcctcct ttgaacccc tcgctagcg tttccttcg 
accgctccg gcgatgtta ccgccggct tttgcccca 
tttccccca gctcaggtg atggcgccc acgctgtcc 
aagccgctg gaggatgtg ccgcattgc acggccgcc 
ttcacgtcc tcgcagcat aatatggcg ttgacggcg 
gccattgcg aatctgccg ccgagtttg cccaaggaa 
gtcgaggcg ctgcctgag gatgaggtc gttccgacg 
actcagagt aatgttttg tcgtcacca agtccttca 
attaagtcg aatagttcg gggaaaaaa tcgccaagt 
aagccgaca cggacgtcg gcacgggtc acctcgcag 
gggaagagc cccaagcaa gacgtggtc aagccgagc 
gggagaggg cgaggtgaa gcgaagaga aattcggtt 
aagtcgaat gctgcaaga gggcgtggc agggctaga 
ggcaggggt agagcacac tctcacaat gatcatgag 
tttatcagc aatacgatt cataataag ttggtgggg 
acggtgtac gatctggac tttgatgac gacatttcg 
agcgaaaac gtcgatttg aaatcgatg agggaacgc 
cgaaagtca gtcgatgtc caaaagacc gaactgaag 
gaaagttcc gactcacca tcgcagaaa aaatacaat 
gacttgaga gagttgcga ccgccgagc ccagtcaag 
gacacaacg ccgaaaccg gccgaagtc gaaccgatc 
ttccccgac atcgtccag ccggtatta ccgggccct 
gtagacatg cgcacctac aacaccaac ttcgaccaa 
agctacaac gaagccaac ctcctccga gcgttcgac 
tccgggaca acggaacca ccagtcaac gaggagatt 
gacgaggag ttcgagaaa gaactccag acggctttg 
acaactccg agtaaaaaa ccggacgaa cctcccgcc 
aatatcaaa gtttcgtta tcggactct cgtaatcaa 
ctcaaagtc aagattaag ggccccatc gccaactac 
tcctcgacc ataccgccg ctacctccc ccggtcacc 
gtttcgagc atttcgaac aacgtcatc agtaacaat 
tcgatcggg gtcagttcg ggaacgtcg aacttgcga 
cgcatgcgc aaaaaagaa ctgctacga cagtactgg 
acgcaggat atgaacatg gatgattcg tcctgtgcg 
cctggtttt aacgctact ccaaccgta cctcccgtc 
aatcgtact attatcact atacctaag gctgtggct 
tcaatgacc agtattccg acaaaagac gattatcgc 
gactaccga accgacgat gtcatcgaa acaaaacaa 
caccgcaag gaggccaag tcacgaccg ggcgccctc 
tcgcgtgaa ctgcgccaa ctagacctg tccttggac 
gaagacgcc ttgcatgaa cgccggcga agtatcgga 
agcaacaat tcgacgaac tacgactct gggacgaac 
aaacgaaga gggcgcccc ccgcgcccg tcgcagcaa 
acaaccggg actccgaaa atcaaaatt aagattggc 
aatagtata gttggtaag attgacgat aaacgggat 
cggataaga ccgccgaag aaacgcctc gccaatata 
aacatgccg agtgtcgaa gacctgaaa cgcgagagt 
atgaagttc cggaagttg gtcatggcc gacttcgac 
gagggtaag gagaagcgg aagaagaaa gacaaaagt 
gagaaacgt aagcggaaa aaggcgaaa cccgaagtg 
cggataatc tcgaatgag agccagaac aagttgatt 
atacgtatc gggaaaaag ggtgagcgg actagtggt 
ggtgaggag ggtggtgct ccgattaag ttgaagttg 
gcgcggtgt caggagggc agcgggtat gtgatgaag 
agcgaggag gcggcgcag tcgccgccg ccgccaccg 
ccgccgccg cccccgacg ttgggcccg ccgccgctg 
cccctcaac aaagactgt gaagttaga tga
    mRNA sequence
    • >TC006853 RA
      tgtcgatgg tggcgccag ctacattgt caatgttgt 
taggcttac caaatggag tctaatgga gtcgattta 
ataaaaatt gcctgaaaa tcaatcgaa acgatggaa 
aacctcaaa gaaatggtt aggcagcgt cgacacact 
gcctgcaca ataaggtgc gatgtcaca gagagcaaa 
aaggcctaa ccgctcaga cgatggatc agttcctgt 
taaaaggcg caaacgtac ggcggcgga ggaggagga 
agcggcctc cttggcggt gtggcagcc ccgctccaa 
cgacctgaa gagcatcta caaccggac gacgaccga 
ggcgccggc cgagctctt ccgcaaaga cctgatcag 
tgcgatgaa gttgcccga ttcggagcc gctggcatc 
gaatgagta ctgggttat tgtcgatca gtggaagca 
ggagtggga aaggggggt ccaggtgcc agtcaatcc 
agattcctt gccggaacc tagcgtcac tgtcacaca 
gaacacgcc actcaagct gcaacatga gttcaaact 
gccaaaaaa caagtacat ccgcatcac caaggacga 
gaacttcaa aatcgagga gcacgccct atccaacgc 
cccggctca atcggaggc ggcttgctc gtacgacct 
ggacgaatg cgacgtctc ctggctgcg catcctcaa 
ctcggagcg ctcccaatg cggccaatc accgatcaa 
ctccgagca actcgagcg cgtaatcga gcaattcga 
gctatgctg ctgggacaa aatccagtc gattttacg 
caacgaaga aggcctggg catcgaata cgacgagaa 
cgtcatctg cgacgtctg tcgctcccc cgactccga 
agaaggcaa cgagatggt cttctgcga cagctgcaa 
catctgcgt ccaccaggc ctgctacgg catcacccg 
gatacccga aggccaatg gctgtgctg cacgtgcca 
cctcagcaa gcggccgaa atgcgtgct ctgccccaa 
caaaggggg cgccatgaa gtgcacgcg ctccggcca 
gaagtgggc gcacgtctc gtgcgccct ctggatacc 
cgaagtgag catcggctg tgtggagaa aatggaacc 
cataacgaa aatctcaag cattccgca gagccgatg 
ggcgctgat ctgtgtcct gtgccggga gagggtcgg 
cgcctgcat ccagtgctc ggtcaagac gtgcaaaac 
ggcgtatca tgtcacctg tgcgttcaa acacggctt 
ggaaatgcg agcgattat cgaagacga gaacgccga 
tgacggagt taagttgcg ctcatattg cgagaaaca 
cagcaagtc gagcaagaa caaggagaa gagtgtgtg 
ttcggggtc ggaggacga cgattcgcg ccggaaaaa 
gcgcaaaga catgacttc ggaggagaa gaaccaggc 
acgtgctgc acggttaca ggagatcga gtcggagtt 
tttcaagca cgtgtcgat taaagacgt cagtatcca 
cctggacgt ggacaacga cgccctcca ccacatcta 
caactactg gaagttgaa gcgcaaagc cgggaacaa 
caagccgct gctgccgcc gaagtccga ggacgttga 
tatgctctc tcataaacg cgaacaggc cgatatgga 
gaagatgaa gatgtttgt gcaattgag acaggactt 
ggaacgggt gcggaattt gtgttatat ggtgagtcg 
gagagagaa actgtcgag gagtttctt tcgattgcg 
cgaacagac gttttataa acagacggc tgtgcttga 
agctgctaa caatttgcc gaatgttgt ggtacaagc 
tgtgattga agccaatca tggaccgtc gatctatga 
tcggttgta ttcgcatga agatgcaga ggatcatac 
gagcgattt tgagaggat tttgacgag gattgctgg 
ggttaagtc gccgacgga ttcagggga tgagaaacg 
gacagagct taatgggct ttttaagga caagaatcc 
atacaaaaa gatttactt caatggttc ttcgaatcg 
acgcagcgg cagtttgta cggcagtag ttcgagtag 
tgacgaaaa ggaccgctt gaacgcggc aacaacttc 
cgaagatga agacaaaag taaaaagaa atcatcttc 
atcggtccg aaaacctaa caacaataa caacaaagc 
gtccgatcg ccgacgaaa aagacacag tgcatctag 
taaaatcga aagtagtag tgaggagga ttccaaaca 
gaaaacgaa aaagacttg gggacaatt ctcccgctt 
aaagcaagt ggaacgcga attaggtta ttcgggtag 
tgacagtga cgaactaat gccaatacg tgcaagcca 
aaaatcaga cactaaaca aatgtcgtc aatctattc 
ggatagtga cagttcgga cgtatcaat gaccaaagc 
gagtgattc ccaacaaaa taaactacg aacgaaagc 
agcagtcaa agagttttc acacaaacc gtcaacgtc 
caacaaaac aagtcccga aaacaaaaa gaacaagaa 
aaaagaaag ccccacaaa gaaaaaaga ttacgaccc 
atctgatct cattgtacc gcagcgaca agcggccaa 
aaaagcaac cgaaaatct caaatcgac aacaacgcg 
agttgtcaa agaacaaat ggacgaacc aagcgtaaa 
agttgaaga aaaacccgc caaattgcc caaacctaa 
agcaaaaac taaagaaac cgtaaagga agtgaaaga 
agtgaagaa ggaatcagc ggtgaaaac gagcgatgt 
gttcgatct cgataaaga aacggacaa tgcggagat 
tctcgccta cgttccgca acgtcaagc tgcgaaaaa 
agccgctga acacataaa aagtgggct gggtaagcc 
cgtggcgcc ctctagtgt tgacgaatt ggagaaaaa 
caaattgga gagtacagt ggtcaagaa aaaggaggc 
ggaaaaaca acagccggc taaagtccc agaaagcaa 
acgcaagtc ttcctcaaa cagcagttc gtcttcctc 
gtctagttc ttccacgtc gtcttcgac gtcgtcgag 
ttcagatag tgatgatga ggttccacc aagtcagca 
acaagcgca acaaccgcg atcggagag tcttttctc 
gaattactc gtcgaaaga gtgtcggga aacggctaa 
gcggacgaa cgccagcaa ggactggcc ctttcttga 
caagggtgc caagtctgc agcagcagc ggcgtccag 
ctcaagcac cgactcgag tgattctag ccgtcccac 
ctccccaat catcacaac aacaacaac aacaaaaac 
tactagtcc cgacaagaa gtcaacaac tactcaaag 
agaggaaca gacgagaat gcgaggaaa aggcagacc 
agcaacaac agcagctag agggaggcc gaggcaatc 
atctaaatc cggtgatcg ggtcggaga tgctaatgt 
caggcttag aaatttgtc tgatagcca acgtggcga 
tgctaagtg tcggaaggg aaaacagga tgaggtggc 
cgagaaaga gacaaagtc cgttaacaa actacagtc 
tccggtccg gaaaacgga caaaaaact aaccgaaag 
caaagaatc gtcggaagt cagtaatct cgaaaagga 
aatactcga acgaaaagc tggaaaaca gggctcatc 
caaaacgaa atcaactct cgaccggtt attcggttc 
accacctga caaaaagga cgaaaagaa accgttaaa 
caaaacgac aaaaccgac caaacctga acgagtaaa 
atcaacagt aaaggaaaa caaagagcc ggaaaaaat 
agcatcacc cgtcaaaaa atcgccggt taaaagcaa 
aaacatcga tgagatttt cacacaaaa gactgtcct 
aaacgagaa taaaatcga ggttaaaga aaaggaaaa 
acacccaac tccgaagcg tgaagaaat cgaaccggc 
gccaaaaat cgaagattt caaacaaaa caacgagaa 
actcaccga accggagaa gaaattgcc gcacagtcg 
gtcaatttt ctcgccacc gcatctcaa agacaattt 
gcttgattt cgataattt ggacgacgg ttttggtat 
ttccaaaga cgaggagat aatgaaagg ccccctgac 
gttcaattt cggcaacac tagtctttt taaggaaga 
ctcaaagga agacagtgc acgtgaaac gctaaatct 
tgtcgaaaa acttcgcat ggaaatgtc gaaaaaatc 
gaccagttg tgatgttga cgatggtgc aagtgtttc 
gtcgagtac taaaaacga atgtgatcg gactgagtt 
tgccgaaca agtggtgcc gaattgtgt ggcggaaaa 
gaacgatgc gaaagtgga tgatgtgcc gccgccgac 
tgtgaactt ggagatgcg tgtggaggt gacggaagc 
gaaaggttt cgccgatag agggcagga ctatcggtt 
tatgagttg tgaggcgga tgttccatt ggaacagaa 
caagaacat tccgattca acagcatca tgggcttag 
tgatcatgg gattagtgg gcagggtga tgagagatg 
ggtgccacc cagcgaggc gtatacggc agcgccacc 
tatagaccc ttcgaatta cggcagcga gagccaaca 
acctcttca aacccctca acacttcat gcagcaata 
ctcactcaa caagactca acccctcct caacgccat 
tcctcccca gatcgaaat gcgcctcca cgacgacac 
cctcaaaaa cccatcccc cacgttaca cgaccgtcg 
aatctcaca aacccccct caacgaaga ctgcatgcc 
cagcccatc gccgtacgc cgacatcca cccccaggc 
caaatgggc cgacagcga aatcatgcc caaccgacg 
ttcttcctc cagctcggc ggcctccac ctccagctc 
gtgttcgcg gcgaaacga cgacgacga cctcaaact 
gcggcccga cgtcatgat ggtcaacca ccaggctct 
cgaaatcgg ctacctgca cccgtttcc acccgaatt 
gttcccgac tttgtccga ttcctcgca atttgtcag 
tccggtaag tctcctcca tcctcctcc tttgaaccc 
ctcgctagc gtttccttc gaccgctcc ggcgatgtt 
accgccggc ttttgcccc atttccccc agctcaggt 
gatggcgcc cacgctgtc caagccgct ggaggatgt 
gccgcattg cacggccgc cttcacgtc ctcgcagca 
taatatggc gttgacggc ggccattgc gaatctgcc 
gccgagttt gcccaagga agtcgaggc gctgcctga 
ggatgaggt cgttccgac gactcagag taatgtttt 
gtcgtcacc aagtccttc aattaagtc gaatagttc 
ggggaaaaa atcgccaag taagccgac acggacgtc 
ggcacgggt cacctcgca ggggaagag ccccaagca 
agacgtggt caagccgag cgggagagg gcgaggtga 
agcgaagag aaattcggt taagtcgaa tgctgcaag 
agggcgtgg cagggctag aggcagggg tagagcaca 
ctctcacaa tgatcatga gtttatcag caatacgat 
tcataataa gttggtggg gacggtgta cgatctgga 
ctttgatga cgacatttc gagcgaaaa cgtcgattt 
gaaatcgat gagggaacg ccgaaagtc agtcgatgt 
ccaaaagac cgaactgaa ggaaagttc cgactcacc 
atcgcagaa aaaatacaa tgacttgag agagttgcg 
accgccgag cccagtcaa ggacacaac gccgaaacc 
ggccgaagt cgaaccgat cttccccga catcgtcca 
gccggtatt accgggccc tgtagacat gcgcaccta 
caacaccaa cttcgacca aagctacaa cgaagccaa 
cctcctccg agcgttcga ctccgggac aacggaacc 
accagtcaa cgaggagat tgacgagga gttcgagaa 
agaactcca gacggcttt gacaactcc gagtaaaaa 
accggacga acctcccgc caatatcaa agtttcgtt 
atcggactc tcgtaatca actcaaagt caagattaa 
gggccccat cgccaacta ctcctcgac cataccgcc 
gctacctcc cccggtcac cgtttcgag catttcgaa 
caacgtcat cagtaacaa ttcgatcgg ggtcagttc 
gggaacgtc gaacttgcg acgcatgcg caaaaaaga 
actgctacg acagtactg gacgcagga tatgaacat 
ggatgattc gtcctgtgc gcctggttt taacgctac 
tccaaccgt acctcccgt caatcgtac tattatcac 
tatacctaa ggctgtggc ttcaatgac cagtattcc 
gacaaaaga cgattatcg cgactaccg aaccgacga 
tgtcatcga aacaaaaca acaccgcaa ggaggccaa 
gtcacgacc gggcgccct ctcgcgtga actgcgcca 
actagacct gtccttgga cgaagacgc cttgcatga 
acgccggcg aagtatcgg aagcaacaa ttcgacgaa 
ctacgactc tgggacgaa caaacgaag agggcgccc 
cccgcgccc gtcgcagca aacaaccgg gactccgaa 
aatcaaaat taagattgg caatagtat agttggtaa 
gattgacga taaacggga tcggataag accgccgaa 
gaaacgcct cgccaatat aaacatgcc gagtgtcga 
agacctgaa acgcgagag tatgaagtt ccggaagtt 
ggtcatggc cgacttcga cgagggtaa ggagaagcg 
gaagaagaa agacaaaag tgagaaacg taagcggaa 
aaaggcgaa acccgaagt gcggataat ctcgaatga 
gagccagaa caagttgat tatacgtat cgggaaaaa 
gggtgagcg gactagtgg tggtgagga gggtggtgc 
tccgattaa gttgaagtt ggcgcggtg tcaggaggg 
cagcgggta tgtgatgaa gagcgagga ggcggcgca 
gtcgccgcc gccgccacc gccgccgcc gcccccgac 
gttgggccc gccgccgct gcccctcaa caaagactg 
tgaagttag atgatagtc gtgtaccat tgatctgta 
atataagta taggtgacc agtgaggca ggttgtaca 
gacgctagc tgctacttc acctgtttt aaatccacg 
ttgttaagc tgtctagtg tattattat gatgtgtag 
gctaagcga gcacatgta tgtcattaa tttcagtgt 
acaaagtca attacagcc gcaaacgtg taaccatat 
ttttgtaca tattgtaag ttatggtat ttgtgaata 
taaatagta ctaatttat atatattaa tatcattta 
aaatgatta actcgtgtt gacaatttt gatagctag 
aatcgttat agagtttca tcagatatt gtacagaac 
aaatggctt gagtgcaat ctttttatc ggttgtttg 
ttgtgagag agccattat tttattatt gtacagtct 
ttattattt taaacgtgc gtctagcga cttgtacta 
taaat
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