| General information | |||
| TC006846 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6203356..6221214 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006846 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01097 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Pupal morphology
- >TC006846 RA
MFHLFTTVT LLLASFDIS LCQRKVSRN VALGGEVKT VANFNCPEE FGYYPHPND CTQYYVCVF GGALLESCT GGLMYSHEL QTCDWPRNV GCDGAEISG PVAATSASP PSSRTREES RTRYTPPTP PPAQPAAIV TSRGQPRQL QHNQQEIIK QRQQQQLYA DAEETLPPA EEVESDRQQ RVYRGQPST IGQVQKDRD GLRHSNAIP TYSGRSERI GVISFGTQQ QQQQQQPQQ QYSTIDDRL SNDLTLKRR RKRQTQRRE PNKLKHTID YYRHENLML PQHTHIINH NTSSIKITN PNINLSTAS TTSTPMYSD SSKEEESNK ATSDYPDFP EQFFGPPAS YFNSKNKYE SIENPFANP HFDFDKFLS GLREEPPTT TVSSPVNKL KSKPKTTTP PKTQPSDYY YEYEDDNEG RRESNDAPK KIYSSQIKP TLKTLPKPT NDYYYEEEY DYPQKNNNK PVNVSKNGE MKYSAKYQG KPLKAIKTT RVPQIATTS MRPTTKQFL TSETFASTS TVSSTTQKP KYTTRRKSK LTVPTTRSR SKPRQRLTD DDKKIRVDT SSTAPVQPS AISTATATA RALYNSSQY NSNNYDPYY ALYDDDVEL YRDDDYSQQ YNNNNNNAA QSQTPHYRR TPSPAIQST QEEAPKRGN SGYIQNNYN NYQPNSNSD YNDDNSYNS QVEDQNAYR QVNRQPTRT STRGRGSTH YANGGNIVN NEVTVTVNR GTPASRPRP TLKPSTSIV SKASEFIDI YRYPPSRPA PIYPQPQPD KTAAKCRKD VCLLPDCSC GGKEIPGDL PVEQVPQLV LLTFDDSVN DLNKGLYSD LFEKGRTNP NGCPIAATF YVSHEWTDY SQVQNLYSD GHEIASHTV SHSFGEQFS QKKWTREVA GQREILSAY GGVHLEDVR GMRAPFLSV GGNKMFKML YDSNFTYDS SMPIYENKP PSWPYTLDY KLFHDCMIP PCPTRSYPG VWEVPMVMW QDLNGGRCS MGDACSNPP DAEGVFKML TKNFQRHYT TNRAPFGLF YHAAWFTQP HHKEGFINF LDSILEMKD VWLVTNWQA IQWVRDPTP ASRLNSFQP FQCDFSNRP KRCNNPKVC NLWHKSGVR YMRTCQPCP DIYPWTGNT GIRSSRIDN DIED
- >TC006846 RB
MFHLFTTVT LLLASFDIS LCQRKVSRN VALGGEVKT VANFNCPEE FGYYPHPND CTQYYVCVF GGALLESCT GGLMYSHEL QTCDWPRNV GCDGAEISG PVAATSASP PSSRTREES RTRYTPPTP PPAQPAAIV TSRGQPRQL QHNQQEIIK QRQQQQLYA DAEETLPPA EEVESDRQQ RVYRGQPST IGQVQKDRD GLRHSNAIP TYSGRSERI GVISFGTQQ QQQQQQPQQ QYSTIDDRL SNDLTLKRR RKRQTQRRE PNKLKHTID YYRHENLML PQHTHIINH NTSSIKITN PNINLSTAS TTSTPMYSD SSKEEESNK ATSDYPDFP EQFFGPPAS YFNSKNKYE SIENPFANP HFDFDKFLS GLREEPPTT TVSSPVNKL KSKPKTTTP PKTQPSDYY YEYEDDNEG RRESNDAPK KIYSSQIKP TLKTLPKPT NDYYYEEEY DYPQKNNNK PVNVSKNGE MKYSAKYQG KPLKAIKTT RVPQIATTS MRPTTKQFL TSETFASTS TVSSTTQKP KYTTRRKSK LTVPTTRSR SKPRQRLTD DDKKIRVDT SSTAPVQPS AISTATATA RALYNSSQY NSNNYDPYY ALYDDDVEL YRDDDYSQQ YNNNNNNAA QSQTPHYRR TPSPAIQST QEEAPKRGN SGYIQNNYN NYQPNSNSD YNDDNSYNS QVEDQNAYR QVNRQPTRT STRGRGSTH YANGGNIVN NEVTVTVNR GTPASRPRP TLKPSTSIV SKASEFIDI YRYPPSRPA PIYPQPQPD KTAAKCRKD VCLLPDCSC GGKEIPGNL PVEEVPQIV LLTFDDAVN DLNKQYYIE LFESGRRNP NGCPITATF YVSHEWTDY SQVQNLYAD GHEMASHTV SHSFGEQFS QKKWTREVA GQREILSAY GGVHLEDVR GMRAPFLSV GGNKMFKML YDSNFTYDS SMPIYENKP PSWPYTLDY KLFHDCMIP PCPTRSYPG VWEVPMVMW QDLNGGRCS MGDACSNPP DAEGVFKML TKNFQRHYT TNRAPFGLF YHAAWFTQP HHKEGFINF LDSILEMKD VWLVTNWQA IQWVRDPTP ASRLNSFQP FQCDFSNRP KRCNNPKVC NLWHKSGVR YMRTCQPCP DIYPWTGNT GIRSSRIDN DIED
- >TC006846 RA
atgtttcat ctatttacc accgttacg cttcttctg gcctctttt gatatttct ttatgtcag cggaaggtc agcagaaat gtagcctta ggaggtgaa gtcaaaact gtcgcaaat tttaactgt ccggaggaa tttggttac taccctcac cccaatgac tgcacgcag tactacgta tgtgtcttc ggaggagct ttgctcgaa tcttgcaca ggtggactc atgtacagc cacgaattg caaacgtgt gactggcca agaaacgta ggatgtgac ggtgccgaa atatcaggt ccagtagca gcgacatca gcgagtcca cccagcagc aggactcgg gaggaatct agaacacgc tacacaccc ccaacacca cccccagct cagccggcg gcaatcgtc acctcccga ggacaaccc agacaactt cagcacaac caacaagaa ataataaaa caacgccaa cagcagcag ctctatgcg gatgcagaa gagactctt ccacccgca gaagaggtc gaaagcgat cgccaacaa agagtctat cggggccaa ccatcaact attggccaa gttcaaaag gacagagat gggttaaga cattcaaat gcaattccg acttacagt ggacgcagt gaacgcatt ggcgttatt tcttttggt actcaacag caacaacaa caacagcaa ccacaacaa cagtacagc acaattgac gacagactt agtaatgac ttgactttg aagaggaga cgtaaaaga cagacgcaa cgaagagaa cccaacaaa ctcaagcac actatagat tactaccga catgaaaac ttaatgttg ccgcaacac acacatata ataaaccac aacacttcc tctatcaaa ataacaaac cccaatatc aacctatca actgccagt accacaagt actcctatg tatagcgac agttcgaaa gaagaggaa agtaacaaa gcgacgtct gattacccc gactttcct gaacaattt ttcggcccc cctgcctcc tatttcaat tcaaaaaac aaatacgaa agcattgaa aatcccttc gcaaatcct cactttgac tttgataaa tttttgtcc ggcttgcgt gaagaaccc ccaactact actgtctca agcccagtt aacaagctc aagtccaaa ccaaaaaca acaactccc ccaaaaacc caaccaagc gactattat tatgaatat gaggacgat aacgagggt agacgtgag tcaaacgac gcgccaaaa aaaatttat tcgagtcaa atcaaaccc acgttaaaa actttaccg aaaccaacc aatgattac tattacgag gaagagtac gactatcct caaaaaaat aataacaaa ccggtcaat gtcagcaaa aatggtgaa atgaaatac tcggcaaaa tatcaagga aaacctctc aaagcaata aaaacgact cgagttcct caaattgca acgacttcg atgcgcccc acgactaag caatttcta acgagtgag acctttgct tcgacctct actgttagt tcgaccaca caaaaacca aaatataca acacgtcgc aaatctaaa ctgacagta ccgacgaca cgttcgaga agcaaacca cgacaaaga ctgactgac gatgacaaa aagataagg gttgatacc agcagtacc gctccagta caaccctca gccatttcc acagccaca gcaacggcc agagctttg tacaacagc agtcagtac aatagcaat aattacgat ccttactat gcgctgtac gatgatgac gtcgagttg tatagagat gacgattac tcgcaacag tataataat aacaataac aacgctgct caaagccaa acacctcac tacaggagg actccttcg ccggcaatc cagtcgact caggaggag gcccccaag cgtggcaac tcgggttat attcagaat aattacaat aattaccag ccgaattca aattccgat tacaacgat gataacagt tacaatagt caagtagaa gatcagaac gcttacaga caagtaaac agacaaccg acaagaacc agtactaga ggcagggga tccacgcac tacgccaat ggagggaac attgtcaac aacgaggtt actgttaca gtcaatagg ggaacccca gcatcgcgg cctcgccca actctgaag ccttccact tctatcgtg tccaaggct tccgaattc atcgacatc tacagatat cctccgtct agacctgcc ccaatttac ccccaaccc cagcctgac aaaactgca gccaagtgc agaaaagac gtgtgcttg ctgcctgac tgcagttgt ggaggaaaa gaaattccg ggcgaccta cctgtcgag caagtgccg cagctcgtg cttctcacc ttcgacgat tcggtgaat gatttgaac aagggcttg tatagcgat ttgtttgaa aaggggaga accaacccc aatggatgt ccaatcgct gcaactttc tacgtttca cacgaatgg actgattac agtcaggtt caaaacttg tattctgat ggtcatgaa attgcgtct catacagta tcgcacagt tttggggaa caattttcc caaaagaaa tggacacgt gaagtggcg ggacagaga gaaatattg tccgcttac gggggcgtt catctggaa gatgtccgc ggaatgaga gctccattt ttgtcggtt ggagggaac aaaatgttc aaaatgttg tacgactca aacttcacc tacgactcg tcaatgcct atttacgag aacaaaccg cccagttgg ccatacacc ctcgattac aaattgttc catgactgc atgatccca ccatgcccc acacgttcc taccccggc gtatgggaa gtgcccatg gttatgtgg caagatcta aacggtggg cgatgctca atgggcgac gcttgtagc aatcctccc gatgccgaa ggcgttttc aaaatgttg accaaaaac ttccaaagg cattacacg accaacaga gccccattt ggactgttc tatcacgct gcctggttc acgcaacca caccacaag gaaggtttc atcaatttc ctcgacagt attttggaa atgaaagat gtgtggttg gtaaccaac tggcaagct atccagtgg gtgcgagac ccaacacca gcatcacga ttaaattcg ttccaaccg ttccagtgt gacttttcc aatcgacca aaacggtgc aacaacccg aaagtgtgc aacttatgg cacaaatct ggagtacgg tacatgaga acttgtcag ccctgccct gatatctac ccctggact ggaaacact ggaatccgc agtagccgc attgacaac gatattgaa gattaa
- >TC006846 RB
atgtttcat ctatttacc accgttacg cttcttctg gcctctttt gatatttct ttatgtcag cggaaggtc agcagaaat gtagcctta ggaggtgaa gtcaaaact gtcgcaaat tttaactgt ccggaggaa tttggttac taccctcac cccaatgac tgcacgcag tactacgta tgtgtcttc ggaggagct ttgctcgaa tcttgcaca ggtggactc atgtacagc cacgaattg caaacgtgt gactggcca agaaacgta ggatgtgac ggtgccgaa atatcaggt ccagtagca gcgacatca gcgagtcca cccagcagc aggactcgg gaggaatct agaacacgc tacacaccc ccaacacca cccccagct cagccggcg gcaatcgtc acctcccga ggacaaccc agacaactt cagcacaac caacaagaa ataataaaa caacgccaa cagcagcag ctctatgcg gatgcagaa gagactctt ccacccgca gaagaggtc gaaagcgat cgccaacaa agagtctat cggggccaa ccatcaact attggccaa gttcaaaag gacagagat gggttaaga cattcaaat gcaattccg acttacagt ggacgcagt gaacgcatt ggcgttatt tcttttggt actcaacag caacaacaa caacagcaa ccacaacaa cagtacagc acaattgac gacagactt agtaatgac ttgactttg aagaggaga cgtaaaaga cagacgcaa cgaagagaa cccaacaaa ctcaagcac actatagat tactaccga catgaaaac ttaatgttg ccgcaacac acacatata ataaaccac aacacttcc tctatcaaa ataacaaac cccaatatc aacctatca actgccagt accacaagt actcctatg tatagcgac agttcgaaa gaagaggaa agtaacaaa gcgacgtct gattacccc gactttcct gaacaattt ttcggcccc cctgcctcc tatttcaat tcaaaaaac aaatacgaa agcattgaa aatcccttc gcaaatcct cactttgac tttgataaa tttttgtcc ggcttgcgt gaagaaccc ccaactact actgtctca agcccagtt aacaagctc aagtccaaa ccaaaaaca acaactccc ccaaaaacc caaccaagc gactattat tatgaatat gaggacgat aacgagggt agacgtgag tcaaacgac gcgccaaaa aaaatttat tcgagtcaa atcaaaccc acgttaaaa actttaccg aaaccaacc aatgattac tattacgag gaagagtac gactatcct caaaaaaat aataacaaa ccggtcaat gtcagcaaa aatggtgaa atgaaatac tcggcaaaa tatcaagga aaacctctc aaagcaata aaaacgact cgagttcct caaattgca acgacttcg atgcgcccc acgactaag caatttcta acgagtgag acctttgct tcgacctct actgttagt tcgaccaca caaaaacca aaatataca acacgtcgc aaatctaaa ctgacagta ccgacgaca cgttcgaga agcaaacca cgacaaaga ctgactgac gatgacaaa aagataagg gttgatacc agcagtacc gctccagta caaccctca gccatttcc acagccaca gcaacggcc agagctttg tacaacagc agtcagtac aatagcaat aattacgat ccttactat gcgctgtac gatgatgac gtcgagttg tatagagat gacgattac tcgcaacag tataataat aacaataac aacgctgct caaagccaa acacctcac tacaggagg actccttcg ccggcaatc cagtcgact caggaggag gcccccaag cgtggcaac tcgggttat attcagaat aattacaat aattaccag ccgaattca aattccgat tacaacgat gataacagt tacaatagt caagtagaa gatcagaac gcttacaga caagtaaac agacaaccg acaagaacc agtactaga ggcagggga tccacgcac tacgccaat ggagggaac attgtcaac aacgaggtt actgttaca gtcaatagg ggaacccca gcatcgcgg cctcgccca actctgaag ccttccact tctatcgtg tccaaggct tccgaattc atcgacatc tacagatat cctccgtct agacctgcc ccaatttac ccccaaccc cagcctgac aaaactgca gccaagtgc agaaaagac gtgtgcttg ctgcctgac tgcagttgt ggaggaaaa gaaattccg ggaaaccta ccggtcgaa gaagttccc caaatcgtt ctccttacg tttgatgac gctgtaaat gatttaaac aaacagtat tatatagag ttattcgaa agtggccgc cgcaatccc aatggttgt cccataacc gcgacattt tatgtttct cacgaatgg actgattat agccaggtc caaaaccta tatgccgat ggtcatgag atggcttca catacagtg tcgcacagt tttggggaa caattttcc caaaagaaa tggacacgt gaagtggcg ggacagaga gaaatattg tccgcttac gggggcgtt catctggaa gatgtccgc ggaatgaga gctccattt ttgtcggtt ggagggaac aaaatgttc aaaatgttg tacgactca aacttcacc tacgactcg tcaatgcct atttacgag aacaaaccg cccagttgg ccatacacc ctcgattac aaattgttc catgactgc atgatccca ccatgcccc acacgttcc taccccggc gtatgggaa gtgcccatg gttatgtgg caagatcta aacggtggg cgatgctca atgggcgac gcttgtagc aatcctccc gatgccgaa ggcgttttc aaaatgttg accaaaaac ttccaaagg cattacacg accaacaga gccccattt ggactgttc tatcacgct gcctggttc acgcaacca caccacaag gaaggtttc atcaatttc ctcgacagt attttggaa atgaaagat gtgtggttg gtaaccaac tggcaagct atccagtgg gtgcgagac ccaacacca gcatcacga ttaaattcg ttccaaccg ttccagtgt gacttttcc aatcgacca aaacggtgc aacaacccg aaagtgtgc aacttatgg cacaaatct ggagtacgg tacatgaga acttgtcag ccctgccct gatatctac ccctggact ggaaacact ggaatccgc agtagccgc attgacaac gatattgaa gattaa
- >TC006846 RA
gatatcatg tcaagcgag ggcggtttg caaggtctt cggcatttt gcgaagtga atgctctat tgtgaaggg aaaatacaa tccggtggg gaattagtt ttctctcaa gtccccact gaaacgttt cacttaaga acaactttt tgattcaat acgacccag tcagttcga gcacgtgtt gcgataagt tcgcttatt gcgcgttct cgccgtcac atttgggga gaattttcg ttccggtat ctacatggg gaagtgttt ttagctcct gcgtgtgac attgcatct ggattatta taagtgcac ttttatgga aatgtttca tctatttac caccgttac gcttcttct ggcctcttt tgatatttc tttatgtca gcggaaggt cagcagaaa tgtagcctt aggaggtga agtcaaaac tgtcgcaaa ttttaactg tccggagga atttggtta ctaccctca ccccaatga ctgcacgca gtactacgt atgtgtctt cggaggagc tttgctcga atcttgcac aggtggact catgtacag ccacgaatt gcaaacgtg tgactggcc aagaaacgt aggatgtga cggtgccga aatatcagg tccagtagc agcgacatc agcgagtcc acccagcag caggactcg ggaggaatc tagaacacg ctacacacc cccaacacc acccccagc tcagccggc ggcaatcgt cacctcccg aggacaacc cagacaact tcagcacaa ccaacaaga aataataaa acaacgcca acagcagca gctctatgc ggatgcaga agagactct tccacccgc agaagaggt cgaaagcga tcgccaaca aagagtcta tcggggcca accatcaac tattggcca agttcaaaa ggacagaga tgggttaag acattcaaa tgcaattcc gacttacag tggacgcag tgaacgcat tggcgttat ttcttttgg tactcaaca gcaacaaca acaacagca accacaaca acagtacag cacaattga cgacagact tagtaatga cttgacttt gaagaggag acgtaaaag acagacgca acgaagaga acccaacaa actcaagca cactataga ttactaccg acatgaaaa cttaatgtt gccgcaaca cacacatat aataaacca caacacttc ctctatcaa aataacaaa ccccaatat caacctatc aactgccag taccacaag tactcctat gtatagcga cagttcgaa agaagagga aagtaacaa agcgacgtc tgattaccc cgactttcc tgaacaatt tttcggccc ccctgcctc ctatttcaa ttcaaaaaa caaatacga aagcattga aaatccctt cgcaaatcc tcactttga ctttgataa atttttgtc cggcttgcg tgaagaacc cccaactac tactgtctc aagcccagt taacaagct caagtccaa accaaaaac aacaactcc cccaaaaac ccaaccaag cgactatta ttatgaata tgaggacga taacgaggg tagacgtga gtcaaacga cgcgccaaa aaaaattta ttcgagtca aatcaaacc cacgttaaa aactttacc gaaaccaac caatgatta ctattacga ggaagagta cgactatcc tcaaaaaaa taataacaa accggtcaa tgtcagcaa aaatggtga aatgaaata ctcggcaaa atatcaagg aaaacctct caaagcaat aaaaacgac tcgagttcc tcaaattgc aacgacttc gatgcgccc cacgactaa gcaatttct aacgagtga gacctttgc ttcgacctc tactgttag ttcgaccac acaaaaacc aaaatatac aacacgtcg caaatctaa actgacagt accgacgac acgttcgag aagcaaacc acgacaaag actgactga cgatgacaa aaagataag ggttgatac cagcagtac cgctccagt acaaccctc agccatttc cacagccac agcaacggc cagagcttt gtacaacag cagtcagta caatagcaa taattacga tccttacta tgcgctgta cgatgatga cgtcgagtt gtatagaga tgacgatta ctcgcaaca gtataataa taacaataa caacgctgc tcaaagcca aacacctca ctacaggag gactccttc gccggcaat ccagtcgac tcaggagga ggcccccaa gcgtggcaa ctcgggtta tattcagaa taattacaa taattacca gccgaattc aaattccga ttacaacga tgataacag ttacaatag tcaagtaga agatcagaa cgcttacag acaagtaaa cagacaacc gacaagaac cagtactag aggcagggg atccacgca ctacgccaa tggagggaa cattgtcaa caacgaggt tactgttac agtcaatag gggaacccc agcatcgcg gcctcgccc aactctgaa gccttccac ttctatcgt gtccaaggc ttccgaatt catcgacat ctacagata tcctccgtc tagacctgc cccaattta cccccaacc ccagcctga caaaactgc agccaagtg cagaaaaga cgtgtgctt gctgcctga ctgcagttg tggaggaaa agaaattcc gggcgacct acctgtcga gcaagtgcc gcagctcgt gcttctcac cttcgacga ttcggtgaa tgatttgaa caagggctt gtatagcga tttgtttga aaaggggag aaccaaccc caatggatg tccaatcgc tgcaacttt ctacgtttc acacgaatg gactgatta cagtcaggt tcaaaactt gtattctga tggtcatga aattgcgtc tcatacagt atcgcacag ttttgggga acaattttc ccaaaagaa atggacacg tgaagtggc gggacagag agaaatatt gtccgctta cgggggcgt tcatctgga agatgtccg cggaatgag agctccatt tttgtcggt tggagggaa caaaatgtt caaaatgtt gtacgactc aaacttcac ctacgactc gtcaatgcc tatttacga gaacaaacc gcccagttg gccatacac cctcgatta caaattgtt ccatgactg catgatccc accatgccc cacacgttc ctaccccgg cgtatggga agtgcccat ggttatgtg gcaagatct aaacggtgg gcgatgctc aatgggcga cgcttgtag caatcctcc cgatgccga aggcgtttt caaaatgtt gaccaaaaa cttccaaag gcattacac gaccaacag agccccatt tggactgtt ctatcacgc tgcctggtt cacgcaacc acaccacaa ggaaggttt catcaattt cctcgacag tattttgga aatgaaaga tgtgtggtt ggtaaccaa ctggcaagc tatccagtg ggtgcgaga cccaacacc agcatcacg attaaattc gttccaacc gttccagtg tgacttttc caatcgacc aaaacggtg caacaaccc gaaagtgtg caacttatg gcacaaatc tggagtacg gtacatgag aacttgtca gccctgccc tgatatcta cccctggac tggaaacac tggaatccg cagtagccg cattgacaa cgatattga agattaatt ctaagggcc acaagaaag taaactgat acaaagttg ttttaagcc aaagcagcg ttattttaa atgttgcta atacataat taaatgcat tttggtttt attctaagt aatggagaa aaattgcac taggtatat cgaacgttg ttattactc ggttctaac gtgacctgt ttgtatgac tcattacta ttacattat ttcaaagct aatagtaca ctagtcatt taaagggtg ccaaatttc ggccatttt gtttcctac ttaatttcg attttattt taaataacg tagcttgct tcagatttc tccattact ttgtaagtg atgttgcaa ttttttgca catgttgta gaggtgtcg cgttcatcg cattatgaa aaaaacgtg atgcacttc atacctcat caacattag gtataccta atagtaaat tgtaaatac tgtagtggt ttattatat tatttaaag atctattac aataatact gccaatcaa aatatactc ttagaatat cctcttacc ttagtcgcc agaaatggt tacaaaatc ctccaaccc gattagttt atgatctaa ttaactata atcttaaaa tacttaatt tattgtgta atatctgaa taatggaag aattcgatt ttttaaatg cgaaccagt gagtgataa taaagtatt ttgttaaga
- >TC006846 RB
gatatcatg tcaagcgag ggcggtttg caaggtctt cggcatttt gcgaagtga atgctctat tgtgaaggg aaaatacaa tccggtggg gaattagtt ttctctcaa gtccccact gaaacgttt cacttaaga acaactttt tgattcaat acgacccag tcagttcga gcacgtgtt gcgataagt tcgcttatt gcgcgttct cgccgtcac atttgggga gaattttcg ttccggtat ctacatggg gaagtgttt ttagctcct gcgtgtgac attgcatct ggattatta taagtgcac ttttatgga aatgtttca tctatttac caccgttac gcttcttct ggcctcttt tgatatttc tttatgtca gcggaaggt cagcagaaa tgtagcctt aggaggtga agtcaaaac tgtcgcaaa ttttaactg tccggagga atttggtta ctaccctca ccccaatga ctgcacgca gtactacgt atgtgtctt cggaggagc tttgctcga atcttgcac aggtggact catgtacag ccacgaatt gcaaacgtg tgactggcc aagaaacgt aggatgtga cggtgccga aatatcagg tccagtagc agcgacatc agcgagtcc acccagcag caggactcg ggaggaatc tagaacacg ctacacacc cccaacacc acccccagc tcagccggc ggcaatcgt cacctcccg aggacaacc cagacaact tcagcacaa ccaacaaga aataataaa acaacgcca acagcagca gctctatgc ggatgcaga agagactct tccacccgc agaagaggt cgaaagcga tcgccaaca aagagtcta tcggggcca accatcaac tattggcca agttcaaaa ggacagaga tgggttaag acattcaaa tgcaattcc gacttacag tggacgcag tgaacgcat tggcgttat ttcttttgg tactcaaca gcaacaaca acaacagca accacaaca acagtacag cacaattga cgacagact tagtaatga cttgacttt gaagaggag acgtaaaag acagacgca acgaagaga acccaacaa actcaagca cactataga ttactaccg acatgaaaa cttaatgtt gccgcaaca cacacatat aataaacca caacacttc ctctatcaa aataacaaa ccccaatat caacctatc aactgccag taccacaag tactcctat gtatagcga cagttcgaa agaagagga aagtaacaa agcgacgtc tgattaccc cgactttcc tgaacaatt tttcggccc ccctgcctc ctatttcaa ttcaaaaaa caaatacga aagcattga aaatccctt cgcaaatcc tcactttga ctttgataa atttttgtc cggcttgcg tgaagaacc cccaactac tactgtctc aagcccagt taacaagct caagtccaa accaaaaac aacaactcc cccaaaaac ccaaccaag cgactatta ttatgaata tgaggacga taacgaggg tagacgtga gtcaaacga cgcgccaaa aaaaattta ttcgagtca aatcaaacc cacgttaaa aactttacc gaaaccaac caatgatta ctattacga ggaagagta cgactatcc tcaaaaaaa taataacaa accggtcaa tgtcagcaa aaatggtga aatgaaata ctcggcaaa atatcaagg aaaacctct caaagcaat aaaaacgac tcgagttcc tcaaattgc aacgacttc gatgcgccc cacgactaa gcaatttct aacgagtga gacctttgc ttcgacctc tactgttag ttcgaccac acaaaaacc aaaatatac aacacgtcg caaatctaa actgacagt accgacgac acgttcgag aagcaaacc acgacaaag actgactga cgatgacaa aaagataag ggttgatac cagcagtac cgctccagt acaaccctc agccatttc cacagccac agcaacggc cagagcttt gtacaacag cagtcagta caatagcaa taattacga tccttacta tgcgctgta cgatgatga cgtcgagtt gtatagaga tgacgatta ctcgcaaca gtataataa taacaataa caacgctgc tcaaagcca aacacctca ctacaggag gactccttc gccggcaat ccagtcgac tcaggagga ggcccccaa gcgtggcaa ctcgggtta tattcagaa taattacaa taattacca gccgaattc aaattccga ttacaacga tgataacag ttacaatag tcaagtaga agatcagaa cgcttacag acaagtaaa cagacaacc gacaagaac cagtactag aggcagggg atccacgca ctacgccaa tggagggaa cattgtcaa caacgaggt tactgttac agtcaatag gggaacccc agcatcgcg gcctcgccc aactctgaa gccttccac ttctatcgt gtccaaggc ttccgaatt catcgacat ctacagata tcctccgtc tagacctgc cccaattta cccccaacc ccagcctga caaaactgc agccaagtg cagaaaaga cgtgtgctt gctgcctga ctgcagttg tggaggaaa agaaattcc gggaaacct accggtcga agaagttcc ccaaatcgt tctccttac gtttgatga cgctgtaaa tgatttaaa caaacagta ttatataga gttattcga aagtggccg ccgcaatcc caatggttg tcccataac cgcgacatt ttatgtttc tcacgaatg gactgatta tagccaggt ccaaaacct atatgccga tggtcatga gatggcttc acatacagt gtcgcacag ttttgggga acaattttc ccaaaagaa atggacacg tgaagtggc gggacagag agaaatatt gtccgctta cgggggcgt tcatctgga agatgtccg cggaatgag agctccatt tttgtcggt tggagggaa caaaatgtt caaaatgtt gtacgactc aaacttcac ctacgactc gtcaatgcc tatttacga gaacaaacc gcccagttg gccatacac cctcgatta caaattgtt ccatgactg catgatccc accatgccc cacacgttc ctaccccgg cgtatggga agtgcccat ggttatgtg gcaagatct aaacggtgg gcgatgctc aatgggcga cgcttgtag caatcctcc cgatgccga aggcgtttt caaaatgtt gaccaaaaa cttccaaag gcattacac gaccaacag agccccatt tggactgtt ctatcacgc tgcctggtt cacgcaacc acaccacaa ggaaggttt catcaattt cctcgacag tattttgga aatgaaaga tgtgtggtt ggtaaccaa ctggcaagc tatccagtg ggtgcgaga cccaacacc agcatcacg attaaattc gttccaacc gttccagtg tgacttttc caatcgacc aaaacggtg caacaaccc gaaagtgtg caacttatg gcacaaatc tggagtacg gtacatgag aacttgtca gccctgccc tgatatcta cccctggac tggaaacac tggaatccg cagtagccg cattgacaa cgatattga agattaatt ctaagggcc acaagaaag taaactgat acaaagttg ttttaagcc aaagcagcg ttattttaa atgttgcta atacataat taaatgcat tttggtttt attctaagt aatggagaa aaattgcac taggtatat cgaacgttg ttattactc ggttctaac gtgacctgt ttgtatgac tcattacta ttacattat ttcaaagct aatagtaca ctagtcatt taaagggtg ccaaatttc ggccatttt gtttcctac ttaatttcg attttattt taaataacg tagcttgct tcagatttc tccattact ttgtaagtg atgttgcaa ttttttgca catgttgta gaggtgtcg cgttcatcg cattatgaa aaaaacgtg atgcacttc atacctcat caacattag gtataccta atagtaaat tgtaaatac tgtagtggt ttattatat tatttaaag atctattac aataatact gccaatcaa aatatactc ttagaatat cctcttacc ttagtcgcc agaaatggt tacaaaatc ctccaaccc gattagttt atgatctaa ttaactata atcttaaaa tacttaatt tattgtgta atatctgaa taatggaag aattcgatt ttttaaatg cgaaccagt gagtgataa taaagtatt ttgttaaga
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