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Gene information
General information
TC006844 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 161428..173122
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006844
      iBeetle screen iB_01096

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Fertility
      mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006844 RA
        MAALKTEIN PQDNEDENE IENEVEEVG AEGAPGEAA 
        KKKKKKKKK KKKAANENS EERVVEDGK IEEAENGAQ 
        DGEGEKKKK KRNRKKGGK LAQTDPPTV PIVDLFPDQ 
        VFPIGEIQE YPTKQDDRT AKDRFTSEE KRALDRMHN 
        DIYNEVRLA AEAHRQTRQ HIQKWIKPG MTMIEICEE 
        LENTARKLI AENGLKAGL AFPTGCSRN HCAAHYTPN 
        AGDPTVLEY DDVVKIDFG THINGRIID CAFTHTFNP 
        KYDKLVEAV RDATNTGIK AAGIDVQLC EIGAAIQEV 
        MESYELELD GKTYPIKSI RNLNGHSIS PYRIHAGKT 
        VPIVKGGEA TVMEENEFY AIETFGSTG RGVVHDDMD 
        CSHYMKNFE SSYVPLRLQ SSKTLLNII NKNFGTLAF 
        CKRWLDRAG ATKYQMALK DLCDKGVVD AYPPLCDIK 
        GCYTAQFEH TIMLRPTCK EVVSRGDDY
      CDS sequence
      • >TC006844 RA
        atggcggcc cttaaaacc gaaataaat cctcaagat 
        aacgaggat gaaaatgaa attgagaac gaagtggaa 
        gaagtaggt gcggaaggg gctcccggt gaagcggcc 
        aaaaagaag aaaaagaag aagaagaag aagaaaaaa 
        gccgccaat gaaaatagc gaagaacgt gttgtggaa 
        gatgggaag attgaagag gcggaaaat ggggcacag 
        gatggcgag ggggaaaag aagaagaag aagcgcaat 
        cggaagaaa ggtggtaaa ctcgctcaa accgatcca 
        ccgactgtt ccaatcgtc gatttattc cccgaccaa 
        gttttccca atcggtgaa atccaggaa tatcccacg 
        aaacaagac gatcgtact gctaaagat cgttttaca 
        tcggaagag aaacgtgct ttagatcgt atgcacaat 
        gacatatac aatgaagta cgtttagct gctgaagca 
        caccgtcaa acgcgtcag catatccaa aaatggatc 
        aaacccggc atgacaatg atcgaaatt tgcgaagaa 
        ttggaaaat acagctcgt aaattaatc gccgaaaat 
        ggtctaaaa gctggactt gcttttcca actggatgt 
        tcgaggaat cattgcgct gcacattat acaccaaat 
        gctggtgat ccaactgtt ttggaatat gacgatgtt 
        gtgaaaatt gattttggg actcatatc aatggacgg 
        attattgat tgtgcgttc acgcatact tttaatccc 
        aagtacgat aagttggtg gaggcggtt cgtgatgcc 
        accaatact gggattaaa gctgcaggg attgatgta 
        caactctgt gagattgga gctgcaatt caagaagtg 
        atggagtcg tatgagctg gaattggat gggaaaact 
        tatccgatt aagtcgatt aggaatctt aatggacat 
        tcgatttcg ccgtatagg atacatgct gggaaaact 
        gttccgatt gttaaagga ggtgaagct actgttatg 
        gaagagaat gagttttat gcgattgaa acttttgga 
        tcgactggt agaggcgtt gttcatgac gatatggat 
        tgttcacac tatatgaag aattttgaa tcttcatat 
        gttccgtta cgtctccaa tcgtcaaaa acccttcta 
        aacattata aacaagaac ttcggaaca ctggcgttc 
        tgtaagcga tggttggac cgcgcaggc gcgaccaaa 
        taccaaatg gctttgaag gatttatgt gacaaaggt 
        gtcgtagac gcataccca cctctatgt gacatcaaa 
        ggttgttac acagcgcaa ttcgaacac accatcatg 
        cttcgtcca acctgcaaa gaagtggtg tcacgtggt 
        gacgattat taa
      mRNA sequence
      • >TC006844 RA
        gcgccccca ttttgtcat ttcgcgcat gcgtgtcac 
        aattttggt tttgtcgct ggtaaaatg gcggccctt 
        aaaaccgaa ataaatcct caagataac gaggatgaa 
        aatgaaatt gagaacgaa gtggaagaa gtaggtgcg 
        gaaggggct cccggtgaa gcggccaaa aagaagaaa 
        aagaagaag aagaagaag aaaaaagcc gccaatgaa 
        aatagcgaa gaacgtgtt gtggaagat gggaagatt 
        gaagaggcg gaaaatggg gcacaggat ggcgagggg 
        gaaaagaag aagaagaag cgcaatcgg aagaaaggt 
        ggtaaactc gctcaaacc gatccaccg actgttcca 
        atcgtcgat ttattcccc gaccaagtt ttcccaatc 
        ggtgaaatc caggaatat cccacgaaa caagacgat 
        cgtactgct aaagatcgt tttacatcg gaagagaaa 
        cgtgcttta gatcgtatg cacaatgac atatacaat 
        gaagtacgt ttagctgct gaagcacac cgtcaaacg 
        cgtcagcat atccaaaaa tggatcaaa cccggcatg 
        acaatgatc gaaatttgc gaagaattg gaaaataca 
        gctcgtaaa ttaatcgcc gaaaatggt ctaaaagct 
        ggacttgct tttccaact ggatgttcg aggaatcat 
        tgcgctgca cattataca ccaaatgct ggtgatcca 
        actgttttg gaatatgac gatgttgtg aaaattgat 
        tttgggact catatcaat ggacggatt attgattgt 
        gcgttcacg catactttt aatcccaag tacgataag 
        ttggtggag gcggttcgt gatgccacc aatactggg 
        attaaagct gcagggatt gatgtacaa ctctgtgag 
        attggagct gcaattcaa gaagtgatg gagtcgtat 
        gagctggaa ttggatggg aaaacttat ccgattaag 
        tcgattagg aatcttaat ggacattcg atttcgccg 
        tataggata catgctggg aaaactgtt ccgattgtt 
        aaaggaggt gaagctact gttatggaa gagaatgag 
        ttttatgcg attgaaact tttggatcg actggtaga 
        ggcgttgtt catgacgat atggattgt tcacactat 
        atgaagaat tttgaatct tcatatgtt ccgttacgt 
        ctccaatcg tcaaaaacc cttctaaac attataaac 
        aagaacttc ggaacactg gcgttctgt aagcgatgg 
        ttggaccgc gcaggcgcg accaaatac caaatggct 
        ttgaaggat ttatgtgac aaaggtgtc gtagacgca 
        tacccacct ctatgtgac atcaaaggt tgttacaca 
        gcgcaattc gaacacacc atcatgctt cgtccaacc 
        tgcaaagaa gtggtgtca cgtggtgac gattattaa 
        ttgtttttg taaatgttt gtcattttg tttacaatt 
        tattgtaaa taaataaaa cgatttttt ttctcattt 
        attattatt gtatttaaa acaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY