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Gene information
General information
TC034941 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid384: 63138..86441
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034941
    iBeetle screen iB_01095
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    iBeetle screen iB_04221
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034941 RA
      MSRGSVSKQ KQSSPSVKN DMDKYEVIK LLGEGSFGR 
      VYKAKVIAD SSFVALKVI CKGRRSNKE IIGLRRECE 
      IQRHLHHPN IIQMLDSFE TPNEIVVVT EFAQKELNT 
      VLGKEGYLT EERASPIIW DLVSALNYL HSHRVLHRD 
      LKPQNILID SKNRAKLCD FGFARNMST GTHVLTSIK 
      GTPLYMAPE LIEEQPYDY KADLWSLGC IMYELLVGA 
      PPFCTASIL HLIRLIRHE QIRWPTLVS ESCVSFLKG 
      LLQKDPLKR INWEQILNH DFVKGHILI CNDSTNMPL 
      TRALSENTL QVQEQQRKE RMTNKVKSS ESKVSTQDL 
      KNNNLSSSV RNDTFIEEN HPIETEEWI VFLQKSMEE 
      VIGGELTSL TQTYLTNII VSPLRNTNT SPKVLVYVA 
      KLLSLPFAL RGISHETKE QIKKVFLEV KLVPNLIYS 
      SKKIMKVLD SSNDETTYK NIADLTEED FEALECVYM 
      LITHLVHLD NEFLSQLCD SIAVLNVYA ILKSFLLIC 
      KNRLQLVMD LLAILTHVL RVFPEHFEL VDRIVLSEG 
      KSVNFVELL RHNNPLMQE RTCFFLLFL GKHLPTNKV 
      EVFWNETVR ETLEALMYD SIGSVRNAA DRTVEELRC 
      KHLPANKVE VFWNETVRE TLEALMYDS IGSVRNADD 
      RTVEELSDE TTYKNIADL TEEDFEALE CVYMLITHL 
      VHLDNEFLS QLCDSIAVL NVYAILKSF LLICKSRLQ 
      LVMDLLAIL THVLRVFPE HFELVDRIV LNEGKSVNF 
      VELLRHNNP LMQERTCFF LLFLGKHLP ANKVEVFWN 
      ETVRETLEA LMYDSIGSV RNAADRTKL ANELELPQI 
      LLFSDLNNT PNNYNEELT PIDLNPTKK VALILTSSG 
      TTGFPKCVQ LTHLNLRTT MLYAMDPNF LDLNQNEST 
      IAFLPYFYV FGCAVSLAS ILSGCKSIV MEKFIPDLF 
      LANIQKHKV TKLFVVPPI LQFLVKNPM VGKFDISSV 
      VDILCGAAV VGKELEEMV QERFKVKSV RQVYGMTEL 
      CGAAAMIPK NFQKYGSSG KVVSCTQIK VCDVANGKT 
      LAAQEIGEI RVKGDGTMK SYLKNEEET KKAFDEEGF 
      LKTGDLGYY DEEGYFYIV DRLKEIIKY KGFQVSPAE 
      LENLLIQHP AVKDAAVVG LPDKRAGEL PLAFVVKQD 
      QNVTEKELI RFISENVSV QKHLYGGVR FIENIPKNS 
      SEKILRLKL QELL
    • >TC034941 RB
      MSRGSVSKQ KQSSPSVKN DMDKYEVIK LLGEGSFGR 
      VYKAKVIAD SSFVALKVI CKGRRSNKE IIGLRRECE 
      IQRHLHHPN IIQMLDSFE TPNEIVVVT EFAQKELNT 
      VLGKEGYLT EERASPIIW DLVSALNYL HSHRVLHRD 
      LKPQNILID SKNRAKLCD FGFARNMST GTHVLTSIK 
      GTPLYMAPE LIEEQPYDY KADLWSLGC IMYELLVGA 
      PPFCTASIL HLIRLIRHE QIRWPTLVS ESCVSFLKG 
      LLQKDPLKR INWEQILNH DFVKGHILI CNDSTNMPL 
      TRALSENTL QVQEQQRKE RMTNKVKSS ESKVSTQDL 
      KNNNLSSSV RNDTFIEEN HPIETEEWI VFLQKSMEE 
      VIGGELTSL TQTYLTNII VSPLRNTNT SPKVLVYVA 
      KLLSLPFAL RGISHETKE QIKKVFLEV KLVPNLIYS 
      SKKIMKVLD SSNDETTYK NIADLTEED FEALECVYM 
      LITHLVHLD NEFLSQLCD SIAVLNVYA ILKSFLLIC 
      KNRLQLVMD LLAILTHVL RVFPEHFEL VDRIVLSEG 
      KSVNFVELL RHNNPLMQE RTCFFLLFL GKHLPTNKV 
      EVFWNETVR ETLEALMYD SIGSVRNAA DRTVEELRC 
      KHLPANKVE VFWNETVRE TLEALMYDS IGSVRNADD 
      RTVEELRCK HLPANKVEV FWNETVRET LEALMYDSI 
      GSVRNAADR TKLANELEL PQILLFSDL NNTPNNYNE 
      ELTPIDLNP TKKVALILT SSGTTGFPK CVQLTHLNL 
      RTTMLYAMD PNFLDLNQN ESTIAFLPY FYVFGCAVS 
      LASILSGCK SIVMEKFIP DLFLANIQK HKVTKLFVV 
      PPILQFLVK NPMVGKFDI SSVVDILCG AAVVGKELE 
      EMVQERFKV KSVRQVYGM TELCGAAAM IPKNFQKYG 
      SSGKVVSCT QIKVCDVAN GKTLAAQEI GEIRVKGDG 
      TMKSYLKNE EETKKAFDE EGFLKTGDL GYYDEEGYF 
      YIVDRLKEI IKYKGFQVS PAELENLLI QHPAVKDAA 
      VVGLPDKRA GELPLAFVV KQDQNVTEK ELIRFISEN 
      VSVQKHLYG GVRFIENIP KNSSEKILR LKLQELL
    CDS sequence
    • >TC034941 RA
      atgtcacgc ggaagtgta agtaaacaa aaacaatcc 
      tctccaagt gtaaaaaat gatatggac aagtatgaa 
      gttattaaa ttattgggc gaaggatcg tttggacga 
      gtttacaaa gcgaaagta atcgcagat tcatctttt 
      gttgcctta aaagttatc tgtaaaggt cgaagatcg 
      aataaggaa ataattggt ctacgtagg gagtgtgaa 
      attcaaaga catttgcat catcccaat attatacaa 
      atgcttgat tcgtttgaa actccaaat gagattgta 
      gtcgtgacc gaattcgcc caaaaagaa ctaaataca 
      gttctgggc aaagaaggt tatttgaca gaagaacgt 
      gcaagtcca attatttgg gacttagta tcagctctc 
      aattattta cattctcat cgtgtttta catcgcgac 
      ctgaaaccg caaaacatt ctaattgat tcaaaaaac 
      cgcgcgaaa ttatgcgac tttggtttt gcgcgaaat 
      atgagcact ggaactcac gtcttaacc tcaattaaa 
      ggaacgcct ctttatatg gcaccagaa ttgattgaa 
      gaacaaccg tatgattat aaagctgac ttgtggtca 
      cttggttgt attatgtac gagttgtta gtgggagcg 
      ccgccattt tgtacggcg tccattttg catttgatt 
      cgattgatt cgacacgaa cagatccga tggcctact 
      ttggtttcg gaaagttgt gtctctttc ctcaaaggg 
      cttttacag aaagatccc ttgaagaga atcaattgg 
      gagcaaatt ttgaatcat gattttgtc aaaggacac 
      attttgatt tgtaatgac tcgactaac atgcccttg 
      actcgagcc ctgtctgaa aatacgcta caagtgcaa 
      gaacagcaa agaaaagag cgaatgact aacaaggtg 
      aaatcaagc gaatcgaaa gtttcaaca caagacttg 
      aaaaacaat aatttatca tcatcggtt cgaaatgac 
      acttttatt gaagaaaat catccgatt gaaactgaa 
      gaatggatt gtttttttg caaaaatca atggaggaa 
      gttatcgga ggtgaactc acttcgttg acacaaaca 
      tacctaact aacataatt gtgtcaccg ttacgtaac 
      actaacaca agtccgaaa gtgttggtt tatgtcgct 
      aaactttta tcgttacct ttcgctctt cgaggaatt 
      tcacacgag accaaggaa caaatcaaa aaagtgttt 
      ctcgaagtg aagttggtt ccaaatttg atttactcc 
      tccaaaaaa ataatgaaa gtcttggac agttccaat 
      gatgaaacg acttacaaa aatatagct gatttaaca 
      gaggaagat tttgaagca cttgaatgt gtctatatg 
      ttgattaca catttggtg catttggat aacgagttt 
      ttgagtcaa ttgtgtgat tccatcgct gttttgaat 
      gtgtatgcg attttgaaa tcgtttctt ttgatttgc 
      aaaaatcga ttacaactg gttatggat ttgttggcg 
      attttgacg catgttttg cgcgttttt cccgaacat 
      tttgaacta gtcgatcga attgttttg agtgaaggg 
      aaaagtgta aattttgtg gaattgttg cgacataat 
      aatcccctg atgcaagaa cggacgtgt ttttttctt 
      ttgttttta ggcaaacat ttgccaacc aataaggtg 
      gaagttttt tggaatgaa actgtgaga gagactttg 
      gaagctcta atgtacgat tcaattgga agtgttagg 
      aatgctgct gatcgcact gttgaagaa cttaggtgc 
      aaacatttg ccagccaat aaggtggaa gttttttgg 
      aatgaaacg gtgagagag actttggaa gctctaatg 
      tatgattcg attggaagt gttaggaat gctgatgat 
      cgcaccgtt gaagaactt agtgatgaa acgacttac 
      aagaatata gctgattta acagaggaa gattttgaa 
      gcacttgaa tgtgtctat atgttgatt acacatttg 
      gtgcatttg gataacgag tttttgagt caattgtgt 
      gattccatc gctgttttg aatgtgtat gcgattttg 
      aaatcgttt cttttgatt tgcaaaagt cgattacaa 
      ctggttatg gatttgttg gcgattttg acgcatgtt 
      ttgcgcgtt tttcccgaa cattttgaa ctagtcgat 
      cgaattgtt ttgaatgaa gggaaaagt gttaatttt 
      gtggaatta ttgcgacat aataatccc ctgatgcaa 
      gaacggacg tgttttttt cttttgttt ttaggcaaa 
      catttgcca gccaataag gtggaagtt ttttggaat 
      gaaactgtg agagagact ttggaagct ctaatgtat 
      gattcgatt ggaagtgtt aggaatgct gctgatcgc 
      accaaacta gcaaacgag ctcgaactc cctcaaatt 
      ttgctcttt tctgaccta aacaacaca ccaaataat 
      tacaacgag gaattaact ccaattgac ttgaacccc 
      accaaaaaa gttgccctc attttaaca tcttctggt 
      accacaggc ttcccaaaa tgcgtccaa ctaacccat 
      ttaaaccta cgcacaaca atgttatat gccatggac 
      ccaaacttc ctcgacttg aaccagaac gaatcaacc 
      atcgctttt ttaccatat ttctacgtt tttggttgt 
      gctgtctct cttgcttcg attctaagt ggttgcaaa 
      tcaatagtt atggaaaaa tttatcccc gatttgttt 
      ttggccaac atacagaag cacaaagtt accaaattg 
      tttgtggtt cctccgatt ttgcaattt ttggttaaa 
      aatccaatg gttgggaaa tttgatatt tcctcagtt 
      gttgatatt ttgtgtggg gctgctgtt gttggtaaa 
      gaattggaa gaaatggtt caggaacgg ttcaaggtc 
      aagtcggtt aggcaagtt tatggtatg acagaatta 
      tgtggtgct gcggccatg attcccaaa aattttcaa 
      aaatatggg tcttccggt aaagtggtt tcctgtacc 
      cagatcaaa gtttgtgac gttgcaaat ggaaaaaca 
      ctggcagct caagaaatt ggggaaata cgggttaaa 
      ggcgatgga accatgaag agttatttg aagaatgaa 
      gaagaaacg aagaaagcg tttgatgaa gaagggttt 
      ttgaagact ggtgatttg ggttattat gatgaggaa 
      ggttatttc tacatcgtt gaccgtttg aaggaaatt 
      atcaaatat aaagggttt caggtatca ccagctgag 
      ttggagaat ttgttgata caacatcct gctgttaaa 
      gatgcagct gttgttgga cttcctgat aaaagagcc 
      ggagagtta cctttggca tttgttgtt aaacaggat 
      caaaatgtg acggaaaaa gaattgatt cgattcatt 
      tcagagaat gtttctgtc cagaaacat ttgtatggt 
      ggagttaga tttattgaa aacattccg aaaaattcc 
      agtgagaaa attctaaga ctaaaacta caagagctg 
      ctatga
    • >TC034941 RB
      atgtcacgc ggaagtgta agtaaacaa aaacaatcc 
      tctccaagt gtaaaaaat gatatggac aagtatgaa 
      gttattaaa ttattgggc gaaggatcg tttggacga 
      gtttacaaa gcgaaagta atcgcagat tcatctttt 
      gttgcctta aaagttatc tgtaaaggt cgaagatcg 
      aataaggaa ataattggt ctacgtagg gagtgtgaa 
      attcaaaga catttgcat catcccaat attatacaa 
      atgcttgat tcgtttgaa actccaaat gagattgta 
      gtcgtgacc gaattcgcc caaaaagaa ctaaataca 
      gttctgggc aaagaaggt tatttgaca gaagaacgt 
      gcaagtcca attatttgg gacttagta tcagctctc 
      aattattta cattctcat cgtgtttta catcgcgac 
      ctgaaaccg caaaacatt ctaattgat tcaaaaaac 
      cgcgcgaaa ttatgcgac tttggtttt gcgcgaaat 
      atgagcact ggaactcac gtcttaacc tcaattaaa 
      ggaacgcct ctttatatg gcaccagaa ttgattgaa 
      gaacaaccg tatgattat aaagctgac ttgtggtca 
      cttggttgt attatgtac gagttgtta gtgggagcg 
      ccgccattt tgtacggcg tccattttg catttgatt 
      cgattgatt cgacacgaa cagatccga tggcctact 
      ttggtttcg gaaagttgt gtctctttc ctcaaaggg 
      cttttacag aaagatccc ttgaagaga atcaattgg 
      gagcaaatt ttgaatcat gattttgtc aaaggacac 
      attttgatt tgtaatgac tcgactaac atgcccttg 
      actcgagcc ctgtctgaa aatacgcta caagtgcaa 
      gaacagcaa agaaaagag cgaatgact aacaaggtg 
      aaatcaagc gaatcgaaa gtttcaaca caagacttg 
      aaaaacaat aatttatca tcatcggtt cgaaatgac 
      acttttatt gaagaaaat catccgatt gaaactgaa 
      gaatggatt gtttttttg caaaaatca atggaggaa 
      gttatcgga ggtgaactc acttcgttg acacaaaca 
      tacctaact aacataatt gtgtcaccg ttacgtaac 
      actaacaca agtccgaaa gtgttggtt tatgtcgct 
      aaactttta tcgttacct ttcgctctt cgaggaatt 
      tcacacgag accaaggaa caaatcaaa aaagtgttt 
      ctcgaagtg aagttggtt ccaaatttg atttactcc 
      tccaaaaaa ataatgaaa gtcttggac agttccaat 
      gatgaaacg acttacaaa aatatagct gatttaaca 
      gaggaagat tttgaagca cttgaatgt gtctatatg 
      ttgattaca catttggtg catttggat aacgagttt 
      ttgagtcaa ttgtgtgat tccatcgct gttttgaat 
      gtgtatgcg attttgaaa tcgtttctt ttgatttgc 
      aaaaatcga ttacaactg gttatggat ttgttggcg 
      attttgacg catgttttg cgcgttttt cccgaacat 
      tttgaacta gtcgatcga attgttttg agtgaaggg 
      aaaagtgta aattttgtg gaattgttg cgacataat 
      aatcccctg atgcaagaa cggacgtgt ttttttctt 
      ttgttttta ggcaaacat ttgccaacc aataaggtg 
      gaagttttt tggaatgaa actgtgaga gagactttg 
      gaagctcta atgtacgat tcaattgga agtgttagg 
      aatgctgct gatcgcact gttgaagaa cttaggtgc 
      aaacatttg ccagccaat aaggtggaa gttttttgg 
      aatgaaacg gtgagagag actttggaa gctctaatg 
      tatgattcg attggaagt gttaggaat gctgatgat 
      cgcaccgtt gaagaactt aggtgcaaa catttgcca 
      gccaataag gtggaagtt ttttggaat gaaactgtg 
      agagagact ttggaagct ctaatgtat gattcgatt 
      ggaagtgtt aggaatgct gctgatcgc accaaacta 
      gcaaacgag ctcgaactc cctcaaatt ttgctcttt 
      tctgaccta aacaacaca ccaaataat tacaacgag 
      gaattaact ccaattgac ttgaacccc accaaaaaa 
      gttgccctc attttaaca tcttctggt accacaggc 
      ttcccaaaa tgcgtccaa ctaacccat ttaaaccta 
      cgcacaaca atgttatat gccatggac ccaaacttc 
      ctcgacttg aaccagaac gaatcaacc atcgctttt 
      ttaccatat ttctacgtt tttggttgt gctgtctct 
      cttgcttcg attctaagt ggttgcaaa tcaatagtt 
      atggaaaaa tttatcccc gatttgttt ttggccaac 
      atacagaag cacaaagtt accaaattg tttgtggtt 
      cctccgatt ttgcaattt ttggttaaa aatccaatg 
      gttgggaaa tttgatatt tcctcagtt gttgatatt 
      ttgtgtggg gctgctgtt gttggtaaa gaattggaa 
      gaaatggtt caggaacgg ttcaaggtc aagtcggtt 
      aggcaagtt tatggtatg acagaatta tgtggtgct 
      gcggccatg attcccaaa aattttcaa aaatatggg 
      tcttccggt aaagtggtt tcctgtacc cagatcaaa 
      gtttgtgac gttgcaaat ggaaaaaca ctggcagct 
      caagaaatt ggggaaata cgggttaaa ggcgatgga 
      accatgaag agttatttg aagaatgaa gaagaaacg 
      aagaaagcg tttgatgaa gaagggttt ttgaagact 
      ggtgatttg ggttattat gatgaggaa ggttatttc 
      tacatcgtt gaccgtttg aaggaaatt atcaaatat 
      aaagggttt caggtatca ccagctgag ttggagaat 
      ttgttgata caacatcct gctgttaaa gatgcagct 
      gttgttgga cttcctgat aaaagagcc ggagagtta 
      cctttggca tttgttgtt aaacaggat caaaatgtg 
      acggaaaaa gaattgatt cgattcatt tcagagaat 
      gtttctgtc cagaaacat ttgtatggt ggagttaga 
      tttattgaa aacattccg aaaaattcc agtgagaaa 
      attctaaga ctaaaacta caagagctg ctatga
    mRNA sequence
    • >TC034941 RA
      aattggcat ttctttgca aaaaattgc tttccaccc 
      agtcagtta ctttcacga gggttggtg ttcctgcct 
      gtcagcgtg acaaacatc tgattgtca aaaaaatgt 
      cacgcggaa gtgtaagta aacaaaaac aatcctctc 
      caagtgtaa aaaatgata tggacaagt atgaagtta 
      ttaaattat tgggcgaag gatcgtttg gacgagttt 
      acaaagcga aagtaatcg cagattcat cttttgttg 
      ccttaaaag ttatctgta aaggtcgaa gatcgaata 
      aggaaataa ttggtctac gtagggagt gtgaaattc 
      aaagacatt tgcatcatc ccaatatta tacaaatgc 
      ttgattcgt ttgaaactc caaatgaga ttgtagtcg 
      tgaccgaat tcgcccaaa aagaactaa atacagttc 
      tgggcaaag aaggttatt tgacagaag aacgtgcaa 
      gtccaatta tttgggact tagtatcag ctctcaatt 
      atttacatt ctcatcgtg ttttacatc gcgacctga 
      aaccgcaaa acattctaa ttgattcaa aaaaccgcg 
      cgaaattat gcgactttg gttttgcgc gaaatatga 
      gcactggaa ctcacgtct taacctcaa ttaaaggaa 
      cgcctcttt atatggcac cagaattga ttgaagaac 
      aaccgtatg attataaag ctgacttgt ggtcacttg 
      gttgtatta tgtacgagt tgttagtgg gagcgccgc 
      cattttgta cggcgtcca ttttgcatt tgattcgat 
      tgattcgac acgaacaga tccgatggc ctactttgg 
      tttcggaaa gttgtgtct ctttcctca aagggcttt 
      tacagaaag atcccttga agagaatca attgggagc 
      aaattttga atcatgatt ttgtcaaag gacacattt 
      tgatttgta atgactcga ctaacatgc ccttgactc 
      gagccctgt ctgaaaata cgctacaag tgcaagaac 
      agcaaagaa aagagcgaa tgactaaca aggtgaaat 
      caagcgaat cgaaagttt caacacaag acttgaaaa 
      acaataatt tatcatcat cggttcgaa atgacactt 
      ttattgaag aaaatcatc cgattgaaa ctgaagaat 
      ggattgttt ttttgcaaa aatcaatgg aggaagtta 
      tcggaggtg aactcactt cgttgacac aaacatacc 
      taactaaca taattgtgt caccgttac gtaacacta 
      acacaagtc cgaaagtgt tggtttatg tcgctaaac 
      ttttatcgt tacctttcg ctcttcgag gaatttcac 
      acgagacca aggaacaaa tcaaaaaag tgtttctcg 
      aagtgaagt tggttccaa atttgattt actcctcca 
      aaaaaataa tgaaagtct tggacagtt ccaatgatg 
      aaacgactt acaaaaata tagctgatt taacagagg 
      aagattttg aagcacttg aatgtgtct atatgttga 
      ttacacatt tggtgcatt tggataacg agtttttga 
      gtcaattgt gtgattcca tcgctgttt tgaatgtgt 
      atgcgattt tgaaatcgt ttcttttga tttgcaaaa 
      atcgattac aactggtta tggatttgt tggcgattt 
      tgacgcatg ttttgcgcg tttttcccg aacattttg 
      aactagtcg atcgaattg ttttgagtg aagggaaaa 
      gtgtaaatt ttgtggaat tgttgcgac ataataatc 
      ccctgatgc aagaacgga cgtgttttt ttcttttgt 
      ttttaggca aacatttgc caaccaata aggtggaag 
      ttttttgga atgaaactg tgagagaga ctttggaag 
      ctctaatgt acgattcaa ttggaagtg ttaggaatg 
      ctgctgatc gcactgttg aagaactta ggtgcaaac 
      atttgccag ccaataagg tggaagttt tttggaatg 
      aaacggtga gagagactt tggaagctc taatgtatg 
      attcgattg gaagtgtta ggaatgctg atgatcgca 
      ccgttgaag aacttagtg atgaaacga cttacaaga 
      atatagctg atttaacag aggaagatt ttgaagcac 
      ttgaatgtg tctatatgt tgattacac atttggtgc 
      atttggata acgagtttt tgagtcaat tgtgtgatt 
      ccatcgctg ttttgaatg tgtatgcga ttttgaaat 
      cgtttcttt tgatttgca aaagtcgat tacaactgg 
      ttatggatt tgttggcga ttttgacgc atgttttgc 
      gcgtttttc ccgaacatt ttgaactag tcgatcgaa 
      ttgttttga atgaaggga aaagtgtta attttgtgg 
      aattattgc gacataata atcccctga tgcaagaac 
      ggacgtgtt tttttcttt tgtttttag gcaaacatt 
      tgccagcca ataaggtgg aagtttttt ggaatgaaa 
      ctgtgagag agactttgg aagctctaa tgtatgatt 
      cgattggaa gtgttagga atgctgctg atcgcacca 
      aactagcaa acgagctcg aactccctc aaattttgc 
      tcttttctg acctaaaca acacaccaa ataattaca 
      acgaggaat taactccaa ttgacttga accccacca 
      aaaaagttg ccctcattt taacatctt ctggtacca 
      caggcttcc caaaatgcg tccaactaa cccatttaa 
      acctacgca caacaatgt tatatgcca tggacccaa 
      acttcctcg acttgaacc agaacgaat caaccatcg 
      cttttttac catatttct acgtttttg gttgtgctg 
      tctctcttg cttcgattc taagtggtt gcaaatcaa 
      tagttatgg aaaaattta tccccgatt tgtttttgg 
      ccaacatac agaagcaca aagttacca aattgtttg 
      tggttcctc cgattttgc aatttttgg ttaaaaatc 
      caatggttg ggaaatttg atatttcct cagttgttg 
      atattttgt gtggggctg ctgttgttg gtaaagaat 
      tggaagaaa tggttcagg aacggttca aggtcaagt 
      cggttaggc aagtttatg gtatgacag aattatgtg 
      gtgctgcgg ccatgattc ccaaaaatt ttcaaaaat 
      atgggtctt ccggtaaag tggtttcct gtacccaga 
      tcaaagttt gtgacgttg caaatggaa aaacactgg 
      cagctcaag aaattgggg aaatacggg ttaaaggcg 
      atggaacca tgaagagtt atttgaaga atgaagaag 
      aaacgaaga aagcgtttg atgaagaag ggtttttga 
      agactggtg atttgggtt attatgatg aggaaggtt 
      atttctaca tcgttgacc gtttgaagg aaattatca 
      aatataaag ggtttcagg tatcaccag ctgagttgg 
      agaatttgt tgatacaac atcctgctg ttaaagatg 
      cagctgttg ttggacttc ctgataaaa gagccggag 
      agttacctt tggcatttg ttgttaaac aggatcaaa 
      atgtgacgg aaaaagaat tgattcgat tcatttcag 
      agaatgttt ctgtccaga aacatttgt atggtggag 
      ttagattta ttgaaaaca ttccgaaaa attccagtg 
      agaaaattc taagactaa aactacaag agctgctat 
      gacgtggat tattaataa atatttctt atatgt
    • >TC034941 RB
      ctgtcagcg tgacaaaca tctgattgt caaaaaaat 
      gtcacgcgg aagtgtaag taaacaaaa acaatcctc 
      tccaagtgt aaaaaatga tatggacaa gtatgaagt 
      tattaaatt attgggcga aggatcgtt tggacgagt 
      ttacaaagc gaaagtaat cgcagattc atcttttgt 
      tgccttaaa agttatctg taaaggtcg aagatcgaa 
      taaggaaat aattggtct acgtaggga gtgtgaaat 
      tcaaagaca tttgcatca tcccaatat tatacaaat 
      gcttgattc gtttgaaac tccaaatga gattgtagt 
      cgtgaccga attcgccca aaaagaact aaatacagt 
      tctgggcaa agaaggtta tttgacaga agaacgtgc 
      aagtccaat tatttggga cttagtatc agctctcaa 
      ttatttaca ttctcatcg tgttttaca tcgcgacct 
      gaaaccgca aaacattct aattgattc aaaaaaccg 
      cgcgaaatt atgcgactt tggttttgc gcgaaatat 
      gagcactgg aactcacgt cttaacctc aattaaagg 
      aacgcctct ttatatggc accagaatt gattgaaga 
      acaaccgta tgattataa agctgactt gtggtcact 
      tggttgtat tatgtacga gttgttagt gggagcgcc 
      gccattttg tacggcgtc cattttgca tttgattcg 
      attgattcg acacgaaca gatccgatg gcctacttt 
      ggtttcgga aagttgtgt ctctttcct caaagggct 
      tttacagaa agatccctt gaagagaat caattggga 
      gcaaatttt gaatcatga ttttgtcaa aggacacat 
      tttgatttg taatgactc gactaacat gcccttgac 
      tcgagccct gtctgaaaa tacgctaca agtgcaaga 
      acagcaaag aaaagagcg aatgactaa caaggtgaa 
      atcaagcga atcgaaagt ttcaacaca agacttgaa 
      aaacaataa tttatcatc atcggttcg aaatgacac 
      ttttattga agaaaatca tccgattga aactgaaga 
      atggattgt ttttttgca aaaatcaat ggaggaagt 
      tatcggagg tgaactcac ttcgttgac acaaacata 
      cctaactaa cataattgt gtcaccgtt acgtaacac 
      taacacaag tccgaaagt gttggttta tgtcgctaa 
      acttttatc gttaccttt cgctcttcg aggaatttc 
      acacgagac caaggaaca aatcaaaaa agtgtttct 
      cgaagtgaa gttggttcc aaatttgat ttactcctc 
      caaaaaaat aatgaaagt cttggacag ttccaatga 
      tgaaacgac ttacaaaaa tatagctga tttaacaga 
      ggaagattt tgaagcact tgaatgtgt ctatatgtt 
      gattacaca tttggtgca tttggataa cgagttttt 
      gagtcaatt gtgtgattc catcgctgt tttgaatgt 
      gtatgcgat tttgaaatc gtttctttt gatttgcaa 
      aaatcgatt acaactggt tatggattt gttggcgat 
      tttgacgca tgttttgcg cgtttttcc cgaacattt 
      tgaactagt cgatcgaat tgttttgag tgaagggaa 
      aagtgtaaa ttttgtgga attgttgcg acataataa 
      tcccctgat gcaagaacg gacgtgttt ttttctttt 
      gtttttagg caaacattt gccaaccaa taaggtgga 
      agttttttg gaatgaaac tgtgagaga gactttgga 
      agctctaat gtacgattc aattggaag tgttaggaa 
      tgctgctga tcgcactgt tgaagaact taggtgcaa 
      acatttgcc agccaataa ggtggaagt tttttggaa 
      tgaaacggt gagagagac tttggaagc tctaatgta 
      tgattcgat tggaagtgt taggaatgc tgatgatcg 
      caccgttga agaacttag gtgcaaaca tttgccagc 
      caataaggt ggaagtttt ttggaatga aactgtgag 
      agagacttt ggaagctct aatgtatga ttcgattgg 
      aagtgttag gaatgctgc tgatcgcac caaactagc 
      aaacgagct cgaactccc tcaaatttt gctcttttc 
      tgacctaaa caacacacc aaataatta caacgagga 
      attaactcc aattgactt gaaccccac caaaaaagt 
      tgccctcat tttaacatc ttctggtac cacaggctt 
      cccaaaatg cgtccaact aacccattt aaacctacg 
      cacaacaat gttatatgc catggaccc aaacttcct 
      cgacttgaa ccagaacga atcaaccat cgctttttt 
      accatattt ctacgtttt tggttgtgc tgtctctct 
      tgcttcgat tctaagtgg ttgcaaatc aatagttat 
      ggaaaaatt tatccccga tttgttttt ggccaacat 
      acagaagca caaagttac caaattgtt tgtggttcc 
      tccgatttt gcaattttt ggttaaaaa tccaatggt 
      tgggaaatt tgatatttc ctcagttgt tgatatttt 
      gtgtggggc tgctgttgt tggtaaaga attggaaga 
      aatggttca ggaacggtt caaggtcaa gtcggttag 
      gcaagttta tggtatgac agaattatg tggtgctgc 
      ggccatgat tcccaaaaa ttttcaaaa atatgggtc 
      ttccggtaa agtggtttc ctgtaccca gatcaaagt 
      ttgtgacgt tgcaaatgg aaaaacact ggcagctca 
      agaaattgg ggaaatacg ggttaaagg cgatggaac 
      catgaagag ttatttgaa gaatgaaga agaaacgaa 
      gaaagcgtt tgatgaaga agggttttt gaagactgg 
      tgatttggg ttattatga tgaggaagg ttatttcta 
      catcgttga ccgtttgaa ggaaattat caaatataa 
      agggtttca ggtatcacc agctgagtt ggagaattt 
      gttgataca acatcctgc tgttaaaga tgcagctgt 
      tgttggact tcctgataa aagagccgg agagttacc 
      tttggcatt tgttgttaa acaggatca aaatgtgac 
      ggaaaaaga attgattcg attcatttc agagaatgt 
      ttctgtcca gaaacattt gtatggtgg agttagatt 
      tattgaaaa cattccgaa aaattccag tgagaaaat 
      tctaagact aaaactaca agagctgct atgacgtgg 
      attattaat aaatatttc ttatatgt