| General information | |||
| TC034190
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14209540..14244204 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034190 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01094 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen dorsoventral-axis shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC034190 RA
MHIYDKFIN KAERLADYG RLLGGSQPS LDGRRRDLD RIQDLFPHD YLGLHIAEP NSTYHVKKS FRKKSLGDL NLIENHNRD TCNRLFLVE APCTMAPLH MPHNAKPRH LFLFSDLLL VAKPRSNTS FKLKESVRV SELWIAPCP DSETGFLLG WPSPARTYL VTFCTQAAR DSWWRELQQ ALEVQLRLE PPTTNIKVV FRDPLSGTE CSKTLGVGP EMNSGDVAR LALDESRIG DWAYTLYAR DRDNAPCAL IGCERPHSI QLARMRQSL CAEEGFDLT HCNNNRIPS DIVFELKPN IKPSPRKTP LKLFRKTTG RLFGVALSR LCTNNALPP AITACLRAL FQRGPHTQG IFRRCASAR ALRELREKV DTQGAAVCD EISNTPALL LAALLKDFL RSLPEPLLT GNAQEWLTA ASSGRTDHL RRLLSLLPR ENHLLLANV VCVLYNIAK RARFNLMSA ANLGVCVGP SLLWESVQN APLRTLPTL VEMLINNCQ ILFGSQVVS LLGEVANDS GAEESDSLH SLGLSLDSV ELSKDQKSL SRDSGLTLS EDDRDSPGL NYNNPSFHR SDWARQAAR VRSLDNTWI EEDEAFCAS NESLCGPPI PPRRPPPLR HLPPVHLPP MYRPPPPPP PIYATAKLL LVTDAESES YV
- >TC034190 RB
MFDNLITRI TSPSSRRRY TYHCPEVHS DPEDNEPVT PRKPRLLER RKKGRRQEN GLRQARSLG RLDGLKEAE RLADYGRLL GGSQPSLDG RRRDLDRIQ DLFPHDYLG LHIAEPNST YHVKKSFRK KSLGDLNLI ENHNRDTCN RLFLVEAPC TMAPLHMPH NAKPRHLFL FSDLLLVAK PRSNTSFKL KESVRVSEL WIAPCPDSE TGFLLGWPS PARTYLVTF CTQAARDSW WRELQQALE VQLRLEPPT TNIKVVFRD PLSGTECSK TLGVGPEMN SGDVARLAL DESRIGDWA YTLYARDRD NAPCALIGC ERPHSIQLA RMRQSLCAE EGFDLTHCN NNRIPSDIV FELKPNIKP SPRKTPLKL FRKTTGRLF GVALSRLCT NNALPPAIT ACLRALFQR GPHTQGIFR RCASARALR ELREKVDTQ GAAVCDEIS NTPALLLAA LLKDFLRSL PEPLLTGNA QEWLTAASS GRTDHLRRL LSLLPRENH LLLANVVCV LYNIAKRAR FNLMSAANL GVCVGPSLL WESVQNAPL RTLPTLVEM LINNCQILF GSQVVSLLG EVANDSGAE ESDSLHSLG LSLDSVELS KDQKSLSRD SGLTLSEDD RDSPGLNYN NPSFHRSDW ARQAARVRS LDNTWIEED EAFCASNES LCGPPIPPR RPPPLRHLP PVHLPPMYR PPPPPPPIY ATAKLLLVT DAESESYV
- >TC034190 RC
MFDNLITRI TSPSSRRRY TYHCPEVHS DPEDNEPVT PRKPRLLER RKKGRRQEN GLRQARSLG RLDGLKEVG PNSPKLAEK LETARN
- >TC034190 RA
atgcacatt tatgacaaa tttataaac aaggccgag cgcttggca gactacggt cgtttattg gggggttcc cagccgtct ctggatggg cgtcggcgc gatctcgat cgcatccaa gacttattt ccccacgat tacctcggt ttacacatc gctgagccg aattcaacg taccacgtg aaaaaatcc ttccggaag aaatcactc ggtgacctc aaccttatc gaaaaccac aacagggac acatgcaac cggctgttt ctggtggag gcgccgtgc actatggca ccgctccac atgccacac aacgccaag cccaggcat ctcttcctc ttctccgac ctgctcctc gtcgccaaa ccgcgctcc aacaccagc tttaaactc aaggagagc gtccgagtg tcggagctc tggatcgcc ccttgtccc gactccgag accggcttc ctcctgggt tggccgtca ccagctcgc acctacttg gtcaccttc tgcacgcaa gcagcgcgc gactcctgg tggcgcgaa ctccaacaa gccctcgaa gtccagctg cgactcgag cccccaacg accaacatc aaggtggtg ttccgcgac cctttaagc ggcaccgaa tgctccaaa acactagga gttggtccc gagatgaat tctggagac gtggcccga ttggcgcta gatgagtcc agaattggg gactgggct tatactcta tatgcccgg gaccgggac aacgcccct tgtgccctc atcggctgc gagcgcccc cacagcata cagctggca aggatgagg cagtcgttg tgcgccgaa gagggcttt gatttaaca cattgtaat aataatcgg ataccgagt gatatcgtc tttgagctc aaaccgaac attaagcca tccccgaga aaaacccct ttgaagctg ttccgcaaa accaccggc cgtttgttc ggagtggct ttatcccgc ctttgcacc aataacgcc ctcccgcct gccatcacc gcctgtctc cgggccctc ttccagagg ggcccccac acccagggt attttccgg cggtgtgcc tcagctcgt gccttgcgt gaactccgg gagaaagtc gatactcaa ggagctgcg gtttgtgac gaaatttca aacacacca gcgctgctc cttgctgca ctccttaaa gacttcctc cggagtctc ccggagcca ctccttacc ggaaacgct caagaatgg ctaaccgcg gcctctagc ggcagaact gatcatttg agaagactc ctcagtttg ttacctcgt gaaaaccac ctgctttta gccaatgtg gtctgcgtc ctttacaac attgctaaa cgggcgagg tttaacctc atgtcagcg gctaattta ggtgtgtgt gttggcccg agtttgctc tgggaatcc gtccaaaac gccccctta aggacgcta cccaccctt gttgagatg ttaattaac aactgccaa attttattc ggttcacaa gtcgtttca ttacttggc gaagtggcg aatgacagc ggggccgag gaatccgac agcttgcac tctctgggt ctgtcgtta gacagtgtc gagttgagc aaagaccag aaatcgcta agtcgcgat tctggcttg accttgtcg gaggacgac cgcgacagt ccaggcctt aactacaac aacccctcg tttcatcgc tctgattgg gcgagacag gccgccaga gtgcgctca ttggacaac acctggatc gaggaagac gaggcgttt tgcgcctcg aacgagagt ttgtgtggg cccccgata ccgccccga cggccgcca cccctaaga cacctgccg ccggtgcat ctgcccccc atgtaccgg ccgccgccg cccccgccc ccgatctac gccacggcg aagctgctc ctcgtcaca gacgccgaa agcgaaagc tacgtctaa
- >TC034190 RB
atgttcgac aacctcata acgcgaatc acgagtcct tcgtcgaga aggcggtac acttatcat tgtccagaa gtgcattct gatccggaa gataacgaa ccggtgacg ccccgaaag cccagattg ctggaaagg aggaaaaaa ggccgccga caggaaaat ggactgcgg caggcgagg agtctcggc cgcctggac ggactcaaa gaggccgag cgcttggca gactacggt cgtttattg gggggttcc cagccgtct ctggatggg cgtcggcgc gatctcgat cgcatccaa gacttattt ccccacgat tacctcggt ttacacatc gctgagccg aattcaacg taccacgtg aaaaaatcc ttccggaag aaatcactc ggtgacctc aaccttatc gaaaaccac aacagggac acatgcaac cggctgttt ctggtggag gcgccgtgc actatggca ccgctccac atgccacac aacgccaag cccaggcat ctcttcctc ttctccgac ctgctcctc gtcgccaaa ccgcgctcc aacaccagc tttaaactc aaggagagc gtccgagtg tcggagctc tggatcgcc ccttgtccc gactccgag accggcttc ctcctgggt tggccgtca ccagctcgc acctacttg gtcaccttc tgcacgcaa gcagcgcgc gactcctgg tggcgcgaa ctccaacaa gccctcgaa gtccagctg cgactcgag cccccaacg accaacatc aaggtggtg ttccgcgac cctttaagc ggcaccgaa tgctccaaa acactagga gttggtccc gagatgaat tctggagac gtggcccga ttggcgcta gatgagtcc agaattggg gactgggct tatactcta tatgcccgg gaccgggac aacgcccct tgtgccctc atcggctgc gagcgcccc cacagcata cagctggca aggatgagg cagtcgttg tgcgccgaa gagggcttt gatttaaca cattgtaat aataatcgg ataccgagt gatatcgtc tttgagctc aaaccgaac attaagcca tccccgaga aaaacccct ttgaagctg ttccgcaaa accaccggc cgtttgttc ggagtggct ttatcccgc ctttgcacc aataacgcc ctcccgcct gccatcacc gcctgtctc cgggccctc ttccagagg ggcccccac acccagggt attttccgg cggtgtgcc tcagctcgt gccttgcgt gaactccgg gagaaagtc gatactcaa ggagctgcg gtttgtgac gaaatttca aacacacca gcgctgctc cttgctgca ctccttaaa gacttcctc cggagtctc ccggagcca ctccttacc ggaaacgct caagaatgg ctaaccgcg gcctctagc ggcagaact gatcatttg agaagactc ctcagtttg ttacctcgt gaaaaccac ctgctttta gccaatgtg gtctgcgtc ctttacaac attgctaaa cgggcgagg tttaacctc atgtcagcg gctaattta ggtgtgtgt gttggcccg agtttgctc tgggaatcc gtccaaaac gccccctta aggacgcta cccaccctt gttgagatg ttaattaac aactgccaa attttattc ggttcacaa gtcgtttca ttacttggc gaagtggcg aatgacagc ggggccgag gaatccgac agcttgcac tctctgggt ctgtcgtta gacagtgtc gagttgagc aaagaccag aaatcgcta agtcgcgat tctggcttg accttgtcg gaggacgac cgcgacagt ccaggcctt aactacaac aacccctcg tttcatcgc tctgattgg gcgagacag gccgccaga gtgcgctca ttggacaac acctggatc gaggaagac gaggcgttt tgcgcctcg aacgagagt ttgtgtggg cccccgata ccgccccga cggccgcca cccctaaga cacctgccg ccggtgcat ctgcccccc atgtaccgg ccgccgccg cccccgccc ccgatctac gccacggcg aagctgctc ctcgtcaca gacgccgaa agcgaaagc tacgtctaa
- >TC034190 RC
atgttcgac aacctcata acgcgaatc acgagtcct tcgtcgaga aggcggtac acttatcat tgtccagaa gtgcattct gatccggaa gataacgaa ccggtgacg ccccgaaag cccagattg ctggaaagg aggaaaaaa ggccgccga caggaaaat ggactgcgg caggcgagg agtctcggc cgcctggac ggactcaaa gaggtaggc cctaattct cccaaacta gcggaaaaa ctggaaact gcgcgtaat tag
- >TC034190 RA
acctcttcc aatgcagtg ctttaatga aaccggtgt gcttttacg ctagcgtga cgatttcca acccggatt atgtgcaag atgcacatt tatgacaaa tttataaac aaggccgag cgcttggca gactacggt cgtttattg gggggttcc cagccgtct ctggatggg cgtcggcgc gatctcgat cgcatccaa gacttattt ccccacgat tacctcggt ttacacatc gctgagccg aattcaacg taccacgtg aaaaaatcc ttccggaag aaatcactc ggtgacctc aaccttatc gaaaaccac aacagggac acatgcaac cggctgttt ctggtggag gcgccgtgc actatggca ccgctccac atgccacac aacgccaag cccaggcat ctcttcctc ttctccgac ctgctcctc gtcgccaaa ccgcgctcc aacaccagc tttaaactc aaggagagc gtccgagtg tcggagctc tggatcgcc ccttgtccc gactccgag accggcttc ctcctgggt tggccgtca ccagctcgc acctacttg gtcaccttc tgcacgcaa gcagcgcgc gactcctgg tggcgcgaa ctccaacaa gccctcgaa gtccagctg cgactcgag cccccaacg accaacatc aaggtggtg ttccgcgac cctttaagc ggcaccgaa tgctccaaa acactagga gttggtccc gagatgaat tctggagac gtggcccga ttggcgcta gatgagtcc agaattggg gactgggct tatactcta tatgcccgg gaccgggac aacgcccct tgtgccctc atcggctgc gagcgcccc cacagcata cagctggca aggatgagg cagtcgttg tgcgccgaa gagggcttt gatttaaca cattgtaat aataatcgg ataccgagt gatatcgtc tttgagctc aaaccgaac attaagcca tccccgaga aaaacccct ttgaagctg ttccgcaaa accaccggc cgtttgttc ggagtggct ttatcccgc ctttgcacc aataacgcc ctcccgcct gccatcacc gcctgtctc cgggccctc ttccagagg ggcccccac acccagggt attttccgg cggtgtgcc tcagctcgt gccttgcgt gaactccgg gagaaagtc gatactcaa ggagctgcg gtttgtgac gaaatttca aacacacca gcgctgctc cttgctgca ctccttaaa gacttcctc cggagtctc ccggagcca ctccttacc ggaaacgct caagaatgg ctaaccgcg gcctctagc ggcagaact gatcatttg agaagactc ctcagtttg ttacctcgt gaaaaccac ctgctttta gccaatgtg gtctgcgtc ctttacaac attgctaaa cgggcgagg tttaacctc atgtcagcg gctaattta ggtgtgtgt gttggcccg agtttgctc tgggaatcc gtccaaaac gccccctta aggacgcta cccaccctt gttgagatg ttaattaac aactgccaa attttattc ggttcacaa gtcgtttca ttacttggc gaagtggcg aatgacagc ggggccgag gaatccgac agcttgcac tctctgggt ctgtcgtta gacagtgtc gagttgagc aaagaccag aaatcgcta agtcgcgat tctggcttg accttgtcg gaggacgac cgcgacagt ccaggcctt aactacaac aacccctcg tttcatcgc tctgattgg gcgagacag gccgccaga gtgcgctca ttggacaac acctggatc gaggaagac gaggcgttt tgcgcctcg aacgagagt ttgtgtggg cccccgata ccgccccga cggccgcca cccctaaga cacctgccg ccggtgcat ctgcccccc atgtaccgg ccgccgccg cccccgccc ccgatctac gccacggcg aagctgctc ctcgtcaca gacgccgaa agcgaaagc tacgtctaa agtctgatg taaataaat gcaatgtcg tggacattg attgtataa gaatttttc cagtttttt tgggaattt gtacatttt gtgaagcca tattatcgt aagttaaga attttgtaa ttgccatgg tatagctta cgcattttt tttataaat ctagagtta gttgaattt tttcaaact ataataccg agaatgtat aatcttgaa at
- >TC034190 RB
tttctaatt taatgaaaa ttacagtta aacctcgtc aataaatca cacgaacaa tttctaatt aaaaacaat tcattttta ccggccgaa aaaaaatcc ccgaatgag cataaatta agatcgacg gttaacgaa accacacga gtgtatcgt aatgttttt gtttcgcca gcgccaact acctgttga acggaaaag atttcaaag tagttgtag ctggagtgt ggatgtggt gtagatgtt ttcaaatta actgtttga taaaccggg acgttgatt taagtgact tttgtgcct ttttatcag tgataaccg cttttttcc atacggatt ttacccatt acctggatt tggccacga ccacttgat tggctggcc agcacgtgc cctaagcac acaccaatc gatcgctaa acgtttggg taaaccgga aaggtcaac atgtccaaa ggaggattc taggaacgc aacatgttc gacaacctc ataacgcga atcacgagt ccttcgtcg agaaggcgg tacacttat cattgtcca gaagtgcat tctgatccg gaagataac gaaccggtg acgccccga aagcccaga ttgctggaa aggaggaaa aaaggccgc cgacaggaa aatggactg cggcaggcg aggagtctc ggccgcctg gacggactc aaagaggcc gagcgcttg gcagactac ggtcgttta ttggggggt tcccagccg tctctggat gggcgtcgg cgcgatctc gatcgcatc caagactta tttccccac gattacctc ggtttacac atcgctgag ccgaattca acgtaccac gtgaaaaaa tccttccgg aagaaatca ctcggtgac ctcaacctt atcgaaaac cacaacagg gacacatgc aaccggctg tttctggtg gaggcgccg tgcactatg gcaccgctc cacatgcca cacaacgcc aagcccagg catctcttc ctcttctcc gacctgctc ctcgtcgcc aaaccgcgc tccaacacc agctttaaa ctcaaggag agcgtccga gtgtcggag ctctggatc gccccttgt cccgactcc gagaccggc ttcctcctg ggttggccg tcaccagct cgcacctac ttggtcacc ttctgcacg caagcagcg cgcgactcc tggtggcgc gaactccaa caagccctc gaagtccag ctgcgactc gagccccca acgaccaac atcaaggtg gtgttccgc gacccttta agcggcacc gaatgctcc aaaacacta ggagttggt cccgagatg aattctgga gacgtggcc cgattggcg ctagatgag tccagaatt ggggactgg gcttatact ctatatgcc cgggaccgg gacaacgcc ccttgtgcc ctcatcggc tgcgagcgc ccccacagc atacagctg gcaaggatg aggcagtcg ttgtgcgcc gaagagggc tttgattta acacattgt aataataat cggataccg agtgatatc gtctttgag ctcaaaccg aacattaag ccatccccg agaaaaacc cctttgaag ctgttccgc aaaaccacc ggccgtttg ttcggagtg gctttatcc cgcctttgc accaataac gccctcccg cctgccatc accgcctgt ctccgggcc ctcttccag aggggcccc cacacccag ggtattttc cggcggtgt gcctcagct cgtgccttg cgtgaactc cgggagaaa gtcgatact caaggagct gcggtttgt gacgaaatt tcaaacaca ccagcgctg ctccttgct gcactcctt aaagacttc ctccggagt ctcccggag ccactcctt accggaaac gctcaagaa tggctaacc gcggcctct agcggcaga actgatcat ttgagaaga ctcctcagt ttgttacct cgtgaaaac cacctgctt ttagccaat gtggtctgc gtcctttac aacattgct aaacgggcg aggtttaac ctcatgtca gcggctaat ttaggtgtg tgtgttggc ccgagtttg ctctgggaa tccgtccaa aacgccccc ttaaggacg ctacccacc cttgttgag atgttaatt aacaactgc caaatttta ttcggttca caagtcgtt tcattactt ggcgaagtg gcgaatgac agcggggcc gaggaatcc gacagcttg cactctctg ggtctgtcg ttagacagt gtcgagttg agcaaagac cagaaatcg ctaagtcgc gattctggc ttgaccttg tcggaggac gaccgcgac agtccaggc cttaactac aacaacccc tcgtttcat cgctctgat tgggcgaga caggccgcc agagtgcgc tcattggac aacacctgg atcgaggaa gacgaggcg ttttgcgcc tcgaacgag agtttgtgt gggcccccg ataccgccc cgacggccg ccaccccta agacacctg ccgccggtg catctgccc cccatgtac cggccgccg ccgcccccg cccccgatc tacgccacg gcgaagctg ctcctcgtc acagacgcc gaaagcgaa agctacgtc taaagtctg atgtaaata aatgcaatg tcgtggaca ttgattgta taagaattt ttccagttt ttttgggaa tttgtacat tttgtgaag ccatattat cgtaagtta agaattttg taattgcca tggtatagc ttacgcatt ttttttata aatctagag ttagttgaa ttttttcaa actataata ccgagaatg tataatctt gaaat
- >TC034190 RC
tttctaatt taatgaaaa ttacagtta aacctcgtc aataaatca cacgaacaa tttctaatt aaaaacaat tcattttta ccggccgaa aaaaaatcc ccgaatgag cataaatta agatcgacg gttaacgaa accacacga gtgtatcgt aatgttttt gtttcgcca gcgccaact acctgttga acggaaaag atttcaaag tagttgtag ctggagtgt ggatgtggt gtagatgtt ttcaaatta actgtttga taaaccggg acgttgatt taagtgact tttgtgcct ttttatcag tgataaccg cttttttcc atacggatt ttacccatt acctggatt tggccacga ccacttgat tggctggcc agcacgtgc cctaagcac acaccaatc gatcgctaa acgtttggg taaaccgga aaggtcaac atgtccaaa ggaggattc taggaacgc aacatgttc gacaacctc ataacgcga atcacgagt ccttcgtcg agaaggcgg tacacttat cattgtcca gaagtgcat tctgatccg gaagataac gaaccggtg acgccccga aagcccaga ttgctggaa aggaggaaa aaaggccgc cgacaggaa aatggactg cggcaggcg aggagtctc ggccgcctg gacggactc aaagaggta ggccctaat tctcccaaa ctagcggaa aaactggaa actgcgcgt aattagagt ctcgtccgc atttatacg catttccca ttaccatta caaatttcg caccgcgag acaaactag tttctatag aaatatgaa gtaaaataa ataaaacat gatggcgag t
