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Gene information
General information
TC006779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14115140..14117513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006779
    iBeetle screen iB_01093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006779 RA
      MAVLAAAQL LDELMGRNR NVAPNEKVK ELNWEDPEY 
      CKYFMVKFC PHDLFVNTR ADLGACSKV HDEEIRKMF 
      QQAKPHRKI HYQEEFIRF CSSMINEVE RKIQKGKMR 
      LALSGKTEL PSISPAQSQ KNQEQIKIL NERINGLEA 
      EAEQAGTDG NVEQAQGLM KLCDQLKEE RDSLRKQIE 
      NGHWNATAE LAAAQEKQM EVCQVCGAF LIVGDAQQR 
      IDDHLMGKQ HVGFARLKS ALEEITAVV QTAKEERRS 
      GRSLAGRES ERERERDRE KRRERSEHR SEKDKEKDR 
      RDKDKERKE RREREAHRT KEREKERER DRKHRSDRD 
      SGRRSHHQY RR
    CDS sequence
    • >TC006779 RA
      atggctgtg ttagcggca gctcagctc ttggacgag 
      ttaatgggg cgcaaccgc aacgtcgcc cccaatgaa 
      aaagtcaaa gaattgaac tgggaggac cctgaatat 
      tgcaagtat ttcatggtt aaattctgc ccccacgac 
      ttattcgtg aacacgcga gctgatttg ggcgcgtgt 
      tcgaaagtc cacgatgaa gaaatccga aaaatgttc 
      caacaagcg aaaccccac cgaaaaatc cactaccag 
      gaggagttt atccgtttt tgctcctcc atgatcaat 
      gaagtcgaa cgtaaaata caaaaaggc aaaatgaga 
      ctagccctg agcgggaaa accgaactc ccgtccata 
      tcacccgca caatcgcaa aaaaaccaa gaacaaatt 
      aaaatccta aacgagcga atcaacggt cttgaggct 
      gaagcagag caggctgga accgacggt aacgtggag 
      caagcccaa ggtttgatg aaactttgc gaccagttg 
      aaggaagaa cgcgactca ttacgcaaa caaatcgaa 
      aatgggcat tggaacgcc acggctgag ttggccgcg 
      gcccaggaa aaacaaatg gaagtgtgt caagtctgt 
      ggggctttt ctcatagtc ggcgacgcc cagcaaagg 
      atagacgac cacttgatg ggcaaacaa cacgtgggt 
      tttgcacgt ttaaaatcc gccttggag gaaatcaca 
      gctgtggtt caaaccgcc aaggaggag agacgctcc 
      ggtcgtagt ctagcaggc cgtgaaagt gagagagag 
      cgcgagcgt gacagagag aaacgacgg gaacgatcg 
      gaacatagg tcggaaaaa gacaaggaa aaggatagg 
      agagacaag gataaggag cggaaggag aggagggag 
      agagaggca cataggacg aaagaacgg gagaaggag 
      agggaacga gaccggaaa catcgctcc gatcgtgat 
      tccgggagg aggagtcat caccagtac cgccgctga
    mRNA sequence
    • >TC006779 RA
      aacctgaca aaaacaaaa ttatttcaa ggccatcaa 
      acaaaaaat ggctgtgtt agcggcagc tcagctctt 
      ggacgagtt aatggggcg caaccgcaa cgtcgcccc 
      caatgaaaa agtcaaaga attgaactg ggaggaccc 
      tgaatattg caagtattt catggttaa attctgccc 
      ccacgactt attcgtgaa cacgcgagc tgatttggg 
      cgcgtgttc gaaagtcca cgatgaaga aatccgaaa 
      aatgttcca acaagcgaa accccaccg aaaaatcca 
      ctaccagga ggagtttat ccgtttttg ctcctccat 
      gatcaatga agtcgaacg taaaataca aaaaggcaa 
      aatgagact agccctgag cgggaaaac cgaactccc 
      gtccatatc acccgcaca atcgcaaaa aaaccaaga 
      acaaattaa aatcctaaa cgagcgaat caacggtct 
      tgaggctga agcagagca ggctggaac cgacggtaa 
      cgtggagca agcccaagg tttgatgaa actttgcga 
      ccagttgaa ggaagaacg cgactcatt acgcaaaca 
      aatcgaaaa tgggcattg gaacgccac ggctgagtt 
      ggccgcggc ccaggaaaa acaaatgga agtgtgtca 
      agtctgtgg ggcttttct catagtcgg cgacgccca 
      gcaaaggat agacgacca cttgatggg caaacaaca 
      cgtgggttt tgcacgttt aaaatccgc cttggagga 
      aatcacagc tgtggttca aaccgccaa ggaggagag 
      acgctccgg tcgtagtct agcaggccg tgaaagtga 
      gagagagcg cgagcgtga cagagagaa acgacggga 
      acgatcgga acataggtc ggaaaaaga caaggaaaa 
      ggataggag agacaagga taaggagcg gaaggagag 
      gagggagag agaggcaca taggacgaa agaacggga 
      gaaggagag ggaacgaga ccggaaaca tcgctccga 
      tcgtgattc cgggaggag gagtcatca ccagtaccg 
      ccgctgatt ggttttctc ttagaccag taagatcaa 
      gcaatccag gtaggtggc tttctagtt ttttggttg 
      taatttctt tttcagcgc cacaggtta gtgattgca 
      taagtgacc ttttttgtt taaaatttg taaatagat 
      tgcgatcca acaaaatta ttacttcgt ggagttctt 
      atcaaaata ataagaatt tcaccaagc acaacaatt 
      ggatcaagt tttcttgac gcttagttg actcgtatt 
      cagatggcg gtacgattt aaaatcaca accacaaaa 
      tattagact ttaaacgtg attttctgt gataattaa 
      ggtggaagt ttaaagttt ctaggccct ataagaaaa 
      taaccctgt agaaagtgc attttttta ttcgaaaat 
      ttgcgtttt gagtacttt tatttagtc cttgagaag 
      gacggggca agaacggtc caattgttg tgttttgag 
      gccttggcg gtgttttaa tgcgagagc taggaaacg 
      gttgactcc atttttgtt tcttctaag ttttcagct 
      gtattcagg tatcgatcg agatggtgt cgccacttg 
      gtcacactc acacattcg gttgttttt gtacttacc 
      tgacatttt aaaagaggt atttataca ttgcacata 
      ggagtattg ctccatgcg gtgtcggtt ttatccttg 
      tttaaaaat aaatactaa aattggaaa atggggcta 
      aaacttgac gctcgctgt gttagttca aataacgcc 
      gttgtaatc gaggtattt atcacgtgt ttggaggaa 
      aagtcttga gcacaacta ttaaataat tctctggtc 
      aattagaga catttcgtc cgaatacgg cgggtcaaa 
      tcgtcaaaa aacctcccc ctgtttcgt atcagcgtc 
      tcatcactg gtctggatt tttgcagtt ggctacaaa 
      caggtgagg taaccatgt cgaggtctc cgacgggcg 
      tttggtctt ctgtgcaat gaggtgatg attgtgttt 
      ccattctat taaattatt tgccgttcc aaatggaag 
      cacagtttt ttatactct tgtaaatta tttaggata 
      ggctacgtt aagttaaat gtatgacac tttacaaat 
      aaactagca atactgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY