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Gene information
General information
TC006775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14023510..14025360
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006775
    iBeetle screen iB_01091

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006775 RA
      MGDKGDSET FDDAVEERV INEEYKIWK KNTPFLYDL 
      VMTHALEWP SLTAQWLPD VTRPEGKDH SIHRLILGT 
      HTSDEQNHL LIASVQLPN EDAQFDASH YDNEKGEFG 
      GFGSVSGKI EIEIKINHE GEVNRARYM PQNPCVIAT 
      KTPSSDVLV FDYTKHPSK PDPNGECHP DLRLRGHQK 
      EGYGLSWNP NLNGYLLSA SDDHTICLW DINATPKEN 
      RIIDAKTIF TGHTAVVED VAWHLLHES LFGSVADDQ 
      KLMIWDTRC NNTSKPSHT VDAHTAEVN CLSFNPYSE 
      FILATGSAD KTVALWDLR NLKLKLHSF ESHKDEIFQ 
      VQWSPHNET ILASSGTDR RLHVWDLSK IGEEQSPED 
      AEDGPPELL FIHGGHTAK ISDFSWNPN EPWVICSVS 
      EDNIMQVWQ MAENIYNDE EPEQQADME TPAN
    CDS sequence
    • >TC006775 RA
      atgggggat aagggagac tccgaaacc ttcgacgac 
      gcggtcgag gagcgagtc atcaacgaa gagtacaag 
      atctggaaa aaaaacacc ccttttttg tacgacctt 
      gtaatgaca catgccctc gagtggccc tctttaacc 
      gcccagtgg ctgcccgat gtaacgcgc cccgagggc 
      aaagaccac tccatccac cgcctaatc ctcggcact 
      cacacctcc gacgagcaa aaccacctc ctgatcgcc 
      agcgtccag ctcccgaac gaagacgct cagttcgat 
      gcctcgcac tatgataac gaaaagggc gagtttggc 
      ggttttggc tcagtttcg gggaaaatt gagattgaa 
      attaaaatc aaccacgaa ggagaggtg aacagggct 
      cgctacatg ccccagaac ccgtgcgtt atagccaca 
      aaaaccccc tcaagcgac gtgctagtg ttcgactat 
      acgaagcac cccagcaag cctgacccc aacggggag 
      tgccacccg gacctgaga ctccggggg caccagaag 
      gagggctac gggctgtcc tggaacccc aacttgaac 
      ggctatttg ctctcggcc agcgatgac cacacgatc 
      tgtctctgg gacattaac gcgaccccg aaagagaac 
      cgaattata gacgcgaag accattttt acggggcac 
      actgccgtg gtggaggac gtcgcctgg caccttctg 
      cacgaaagt ctcttcggt tcggtcgca gacgaccag 
      aaattgatg atttgggac actaggtgt aacaatacg 
      agcaaaccg tcgcacact gtcgatgca cacactgct 
      gaggttaac tgtctcagt ttcaacccc tattcggag 
      tttattctt gcaactggg agcgctgat aagactgta 
      gctttgtgg gatttgagg aatcttaag ttgaaattg 
      cacagtttt gagtcgcac aaggatgag attttccaa 
      gtgcagtgg agcccccat aatgagact attctggcg 
      tcgtcgggg accgatcgc aggctgcat gtctgggat 
      ttgagcaaa atcggagag gagcaaagt ccggaggat 
      gcagaggat gggcctccg gagctcctc ttcatacat 
      gggggccac acggccaaa atttcagat tttagttgg 
      aatcccaac gaaccgtgg gttatttgt tccgtgtct 
      gaggataat attatgcaa gtgtggcaa atggccgag 
      aatatttat aacgatgag gagcccgag caacaggcc 
      gatatggaa actccagcg aattag
    mRNA sequence
    • >TC006775 RA
      ccaccgcca aaattttgg ccacggttt gcttaattt 
      gaatctgct gacttgtgg cgttgtggc accactgtt 
      ccaaattgg cgggctagc gtcatattt agtggacgg 
      ctcattcat ttttacttg aatttatca attttaaca 
      ttattcgtt tgtgttcgt tgtaacaat gggggataa 
      gggagactc cgaaacctt cgacgacgc ggtcgagga 
      gcgagtcat caacgaaga gtacaagat ctggaaaaa 
      aaacacccc ttttttgta cgaccttgt aatgacaca 
      tgccctcga gtggccctc tttaaccgc ccagtggct 
      gcccgatgt aacgcgccc cgagggcaa agaccactc 
      catccaccg cctaatcct cggcactca cacctccga 
      cgagcaaaa ccacctcct gatcgccag cgtccagct 
      cccgaacga agacgctca gttcgatgc ctcgcacta 
      tgataacga aaagggcga gtttggcgg ttttggctc 
      agtttcggg gaaaattga gattgaaat taaaatcaa 
      ccacgaagg agaggtgaa cagggctcg ctacatgcc 
      ccagaaccc gtgcgttat agccacaaa aaccccctc 
      aagcgacgt gctagtgtt cgactatac gaagcaccc 
      cagcaagcc tgaccccaa cggggagtg ccacccgga 
      cctgagact ccgggggca ccagaagga gggctacgg 
      gctgtcctg gaaccccaa cttgaacgg ctatttgct 
      ctcggccag cgatgacca cacgatctg tctctggga 
      cattaacgc gaccccgaa agagaaccg aattataga 
      cgcgaagac catttttac ggggcacac tgccgtggt 
      ggaggacgt cgcctggca ccttctgca cgaaagtct 
      cttcggttc ggtcgcaga cgaccagaa attgatgat 
      ttgggacac taggtgtaa caatacgag caaaccgtc 
      gcacactgt cgatgcaca cactgctga ggttaactg 
      tctcagttt caaccccta ttcggagtt tattcttgc 
      aactgggag cgctgataa gactgtagc tttgtggga 
      tttgaggaa tcttaagtt gaaattgca cagttttga 
      gtcgcacaa ggatgagat tttccaagt gcagtggag 
      cccccataa tgagactat tctggcgtc gtcggggac 
      cgatcgcag gctgcatgt ctgggattt gagcaaaat 
      cggagagga gcaaagtcc ggaggatgc agaggatgg 
      gcctccgga gctcctctt catacatgg gggccacac 
      ggccaaaat ttcagattt tagttggaa tcccaacga 
      accgtgggt tatttgttc cgtgtctga ggataatat 
      tatgcaagt gtggcaaat ggccgagaa tatttataa 
      cgatgagga gcccgagca acaggccga tatggaaac 
      tccagcgaa ttagtctag tttttttat ttatgttta 
      gttttagtg ttacctgta agcatacca cgttctcgt 
      ttcctttac tgaagtaat taagtgaaa gtgtgacca 
      tttgtgttt atcaaactg aaacattat gttatccac 
      aatatttta aacattttt tacaaataa ataaatact 
      ttgtccaga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY