Status
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Gene information
General information
TC006769 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13942755..13946625
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006769
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006769 RA
      MRFILVAVL AGLCAVSAI STSESSNYS QQQRDVWRL 
      FKYINQPSY YKDHVEIAH SYYFYDHAS NYAKHEVVE 
      EFYRYFKYD TFLQRGEIF SVFHGEHLK QAIALFKLF 
      YYANDFDTF YKTAVWARQ HVNEGMFLY AFSVALIHR 
      PDTYYFSLP PIYEIYPHY FYNYEVIQK AQHYKQMYY 
      GQDGAHYND RTIYANYSG YYVNVYPEQ ALAYFTEDV 
      GVNSFYYYY NLYYPYWMS GEEFNLKYD NRGEIFYYM 
      YQQILARYY LERLSHGFG EIDHFDWEV PFESGYYPS 
      MCYPNGLYF PTRHAYAHL YEYFYNYGQ HYGFNKYAH 
      SYTHISDYE RRIHDVIDS GYVHTHSGQ KVDLFSHEG 
      LDILGNLIE GNPESPYYH YYGAYQVFA RHLLGYSHQ 
      PLTFHKLHP SALEHFETS MRDPAFYQL YKKLLGFFF 
      RYKSQHYHY YDEHDLAYH GVHVKHVEV DPLVTYFDY 
      FYADLSNAV YVTPEEFVH DSFKVHVAQ ERLNHKPFT 
      YKIYIDSDK DTEAVVKVF LGPKYDEYG RYINLTENW 
      MNFVQFDHF VYKLKSGEN VISRNSHEI YNYIHDRTS 
      YYELYQKAF GVYNKDQHF QFHDNQFFF GFPQRYMLP 
      RGSPEGMTY QFYVFVTKY HPYKAHASV PMVGSGMHY 
      VDAYPMGYP FDRPVYYEE LFYALPNSY FQDVRIYYQ 
      GHNYEHHAN HLIEM
    CDS sequence
    • >TC006769 RA
      atgagattc attttggta gctgttctg gccggcctt 
      tgcgccgtt tctgcgatc tcaacgagt gagtctagc 
      aactactcg caacaacag agggatgtc tggagactc 
      ttcaaatac atcaaccag ccaagctac tacaaggac 
      catgtcgag attgcacac agctactac ttctacgac 
      catgccagc aactacgcc aaacacgaa gttgtggaa 
      gaattctac cggtacttc aagtacgac acgttcctc 
      caacgtggc gaaatcttc tccgttttc cacggcgaa 
      cacttgaag caagccatt gccttgttc aaactcttc 
      tactacgcc aatgacttt gacaccttc tacaaaact 
      gccgtttgg gcgcgacaa cacgtcaac gaaggcatg 
      ttcttgtac gccttctcc gtggctctc atccaccga 
      cctgacact tactacttc tctctgcca ccaatctac 
      gaaatctac cctcattac ttctacaac tacgaggtt 
      atccagaag gcccaacac tacaaacag atgtactat 
      ggacaagac ggcgcccac tacaacgac aggaccatc 
      tatgccaac tattcggga tactatgtt aacgtttac 
      cctgaacag gccttggcc tacttcact gaagatgtg 
      ggtgtcaac tccttctac tactactac aacttgtac 
      tacccgtac tggatgagt ggcgaagaa ttcaacttg 
      aaatatgat aaccgcgga gaaattttc tactacatg 
      taccaacaa atcctagcc aggtactac ctggaacgt 
      ttgtctcat ggttttggc gagattgat catttcgac 
      tgggaagta cctttcgaa agtgggtac tacccatca 
      atgtgctac cccaatggg ctttacttc cccaccaga 
      cacgcctat gcccatttg tacgaatat ttctacaac 
      tatggacaa cactacggt ttcaacaaa tacgcccac 
      agctacacc catatcagc gattatgag aggaggatc 
      catgatgtc atcgacagt ggttacgtc catacccac 
      tctggccaa aaagtcgac ttgttctcg cacgaaggc 
      ctcgacatc ttgggcaac ctcatcgaa ggcaacccc 
      gaatccccc tactaccac tactacgga gcgtaccaa 
      gtgttcgcc cgccacttg ctgggctac tcgcaccaa 
      cccctcacc ttccacaaa ctccaccct tcggccctc 
      gaacacttc gagacctca atgcgcgac ccagctttc 
      taccaactg tacaagaaa ctcctcggc ttcttcttc 
      agatacaag agccagcac taccactac tacgacgag 
      cacgacctt gcctaccac ggcgtccac gtcaaacac 
      gtcgaagtc gaccccttg gtcacctac ttcgactac 
      ttctacgcc gacctgagc aacgccgtc tacgtcacc 
      cccgaggag ttcgtccac gacagcttc aaggtccac 
      gtcgcccaa gagcgcctc aaccacaag cccttcacc 
      tacaagatc tacatcgac tctgacaag gacaccgaa 
      gctgtcgtc aaagtcttc ctggggcca aaatacgac 
      gagtacgga cgttacatt aacctgacc gaaaactgg 
      atgaacttt gtccaattc gaccacttc gtctacaag 
      ctcaagtct ggggaaaac gtaatttcc agaaacagc 
      cacgaaatc tacaactac atccacgac aggacttcc 
      tattacgaa ctgtaccag aaggccttc ggcgtctac 
      aacaaggac caacacttc cagttccac gacaaccag 
      ttcttcttc ggcttccct caaaggtac atgctgccc 
      agaggctct cccgaggga atgacctac cagttctac 
      gtcttcgtc accaagtac cacccatac aaggcgcac 
      gctagcgtg cccatggtg gggtccggc atgcactac 
      gtggacgcc taccccatg ggctacccc ttcgaccgc 
      cctgtctac tacgaggag ctgttctac gccttgccc 
      aactcctac ttccaggat gtcaggatc tactaccaa 
      gggcacaac tacgagcat catgccaac catttaatc 
      gaaatgtaa
    mRNA sequence
    • >TC006769 RA
      ataaaaggg aaaagcgac aaatttgga tcattctcg 
      tcggttggt ccaaccaca aacatgaga ttcattttg 
      gtagctgtt ctggccggc ctttgcgcc gtttctgcg 
      atctcaacg agtgagtct agcaactac tcgcaacaa 
      cagagggat gtctggaga ctcttcaaa tacatcaac 
      cagccaagc tactacaag gaccatgtc gagattgca 
      cacagctac tacttctac gaccatgcc agcaactac 
      gccaaacac gaagttgtg gaagaattc taccggtac 
      ttcaagtac gacacgttc ctccaacgt ggcgaaatc 
      ttctccgtt ttccacggc gaacacttg aagcaagcc 
      attgccttg ttcaaactc ttctactac gccaatgac 
      tttgacacc ttctacaaa actgccgtt tgggcgcga 
      caacacgtc aacgaaggc atgttcttg tacgccttc 
      tccgtggct ctcatccac cgacctgac acttactac 
      ttctctctg ccaccaatc tacgaaatc taccctcat 
      tacttctac aactacgag gttatccag aaggcccaa 
      cactacaaa cagatgtac tatggacaa gacggcgcc 
      cactacaac gacaggacc atctatgcc aactattcg 
      ggatactat gttaacgtt taccctgaa caggccttg 
      gcctacttc actgaagat gtgggtgtc aactccttc 
      tactactac tacaacttg tactacccg tactggatg 
      agtggcgaa gaattcaac ttgaaatat gataaccgc 
      ggagaaatt ttctactac atgtaccaa caaatccta 
      gccaggtac tacctggaa cgtttgtct catggtttt 
      ggcgagatt gatcatttc gactgggaa gtacctttc 
      gaaagtggg tactaccca tcaatgtgc taccccaat 
      gggctttac ttccccacc agacacgcc tatgcccat 
      ttgtacgaa tatttctac aactatgga caacactac 
      ggtttcaac aaatacgcc cacagctac acccatatc 
      agcgattat gagaggagg atccatgat gtcatcgac 
      agtggttac gtccatacc cactctggc caaaaagtc 
      gacttgttc tcgcacgaa ggcctcgac atcttgggc 
      aacctcatc gaaggcaac cccgaatcc ccctactac 
      cactactac ggagcgtac caagtgttc gcccgccac 
      ttgctgggc tactcgcac caacccctc accttccac 
      aaactccac ccttcggcc ctcgaacac ttcgagacc 
      tcaatgcgc gacccagct ttctaccaa ctgtacaag 
      aaactcctc ggcttcttc ttcagatac aagagccag 
      cactaccac tactacgac gagcacgac cttgcctac 
      cacggcgtc cacgtcaaa cacgtcgaa gtcgacccc 
      ttggtcacc tacttcgac tacttctac gccgacctg 
      agcaacgcc gtctacgtc acccccgag gagttcgtc 
      cacgacagc ttcaaggtc cacgtcgcc caagagcgc 
      ctcaaccac aagcccttc acctacaag atctacatc 
      gactctgac aaggacacc gaagctgtc gtcaaagtc 
      ttcctgggg ccaaaatac gacgagtac ggacgttac 
      attaacctg accgaaaac tggatgaac tttgtccaa 
      ttcgaccac ttcgtctac aagctcaag tctggggaa 
      aacgtaatt tccagaaac agccacgaa atctacaac 
      tacatccac gacaggact tcctattac gaactgtac 
      cagaaggcc ttcggcgtc tacaacaag gaccaacac 
      ttccagttc cacgacaac cagttcttc ttcggcttc 
      cctcaaagg tacatgctg cccagaggc tctcccgag 
      ggaatgacc taccagttc tacgtcttc gtcaccaag 
      taccaccca tacaaggcg cacgctagc gtgcccatg 
      gtggggtcc ggcatgcac tacgtggac gcctacccc 
      atgggctac cccttcgac cgccctgtc tactacgag 
      gagctgttc tacgccttg cccaactcc tacttccag 
      gatgtcagg atctactac caagggcac aactacgag 
      catcatgcc aaccattta atcgaaatg taaaggctg 
      attttacga taacggcaa tgtattgag gataattct 
      agaaataaa gtgcaacag tgattccaa tttttctgt 
      ctttttatt ccaactttt ttattaaaa atcatccgt 
      caga
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