Status
Please wait ...
Gene information
General information
TC006768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13914105..13917332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006768
    iBeetle screen iB_01089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006768 RA
      MGQNESKLK LQQITDSLG RIRKDVRSG KITANEVEH 
      FQAILHRYS DEIAANPGR KARTSGVYT AEQLIEITS 
      KELDALKQE DQGATNSLT RTSSKKYTK DKEKDVKSI 
      IASVEQIRE LVRNGQVQA QDFDSYQAR LADYSHRLS 
      FISKKSKKE EVNSAQKLA SLATTELLQ LQEEKNDKV 
      ATPPSQRST MRRKIANRH SPGNLEDIE TKLEALKSD 
      VKVAETNRD KTVLHFLSK SIQTLMTDL QMIKVQDDI 
      ITDERKLLL EKNLIRLNQ QIDKLKRQL NTESNISYL 
      AELQQKDKR EKLLKDLDD IEKSVLAIS RNLSSVNFE 
      SEKESFNKY KAQLSTIEE LSQEISLKK RQVSDVILN 
      IENNFTRLE MEERLNQAE FKHFQTRID LDNFTATSD 
      SDLYKKKRQ FLVQLLDHV AGIMITGDK KNYVLYNIE 
      NNIKALDWK VSQNDEKLN RDEEKDEFV VLRPNLLKV 
      CREIPARGE MSEILAEAR AIIEQKLQI IAGASAQLV 
      TVKEDFLQP VQNTRSVLE DVQKEVETL QLHIEGARY 
      TRESQLYKD LKEKLEGCK ERVRNLDPN ADSLLKYID 
      NVSLLLENN LSSRKSTTE DILVDIMSV STRVNDLGG 
      KLQGVTNSL DFDSIRSSL LEHQQYLRN LHISENRSS 
      LNKSREDIL QQIEAYLRK LDELRVKID RGSEEERRF 
      NQSLRKVKE KVNRYSGTY RNVLYNAIE RDLDKHLRD 
      VDRVFKDSN LALKVRRDI ENCKMILEQ KSSKNNNVK 
      RVELAEVRQ NVESVKEKL TKIQPLTEE ELAQLDARL 
      TLSLLALDN FKDDDSSER AQLYKEIKF YGARIQELK 
      LVPKPHSVL VEDLQQKQL ELQQLAARK IAQIENELA 
      EIKPEIATF VGSETSPKF LALDEALVK ATVRLDELG 
      IEQTSELHH LKVKLMREI QELAEVLDE RVKEAEEIE 
      KYEKEVQVM LIKIDSFNG FFGDAEYTY LDEEIIRLK 
      INLGKLRVN QDLVARKEE CMREIDACM RKLDEAAKT 
      RSDAFEGTL V
    CDS sequence
    • >TC006768 RA
      atgggtcaa aatgagtcg aaactcaag ctgcaacaa 
      atcaccgac tctctaggt cgcatccga aaggacgta 
      cgaagcggt aaaatcacg gcaaatgag gtcgaacac 
      ttccaagcg attttacac cgatattcg gacgaaatc 
      gccgcaaat ccaggccgt aaagcacgc actagtggc 
      gtttacaca gccgaacaa cttatcgaa atcacctcc 
      aaagagctg gacgccctc aaacaagag gaccaaggc 
      gccaccaac tcactcaca cgaacttcg tccaaaaaa 
      tacaccaaa gacaaggaa aaagacgtc aaatcaatc 
      atagcctca gtggagcaa atccgcgag ttggtgcga 
      aatggccaa gtccaagcc caagatttt gacagttac 
      caggcccgt ctggccgat tattcacac aggcttagc 
      ttcatttcg aaaaaatcc aaaaaagag gaagtcaat 
      tcggcccaa aaactggct tctttggcc accaccgag 
      ttgttgcaa ctgcaggag gaaaaaaat gacaaagtt 
      gcaacacca ccatcacaa cggtcgaca atgaggcgc 
      aaaattgcc aacagacac agtcctggc aatttggag 
      gacattgaa accaaactg gaggctctg aagagtgac 
      gttaaagtg gccgaaacg aatcgggac aaaaccgtg 
      ctacatttc ttgtccaag tcgatccaa accctaatg 
      actgacttg caaatgatc aaggtccag gacgatata 
      atcacggat gagagaaaa ctacttttg gagaagaat 
      ttgattcgt ttgaaccaa caaatcgac aagctcaag 
      cggcagttg aacactgaa agtaacatc agttattta 
      gccgagtta caacaaaaa gacaagcgg gagaagttg 
      ctcaaggac ttggacgac atcgaaaag tctgttctg 
      gcaatcagt cgtaaccta tcgagtgtc aatttcgaa 
      tcagagaag gaaagtttt aacaaatac aaagcccaa 
      ctgagcaca atcgaggaa cttagccag gaaatcagt 
      ctgaagaag cgccaagtc tcggacgtg atcctcaac 
      attgagaac aacttcacc cgcttggag atggaggag 
      cgcttgaac caggccgag ttcaagcac ttccagacc 
      cgaattgac cttgacaat ttcaccgca acctccgat 
      agtgacttg tacaaaaaa aagcgccag tttttggtc 
      cagttgctg gaccacgtc gctggaatt atgatcacc 
      ggggacaag aaaaactac gtcttgtac aacatcgag 
      aataatatt aaggcactt gactggaaa gtgtcccaa 
      aacgacgaa aaactgaac cgggacgaa gaaaaggac 
      gagtttgtg gttttgcgc cccaacttg ctaaaagtc 
      tgccgcgag attccagcc aggggggaa atgtccgag 
      attttggcc gaagctagg gccataatt gagcaaaag 
      ttgcaaata attgcgggg gcaagcgcc cagctggtg 
      acagtcaag gaggatttc ctccaaccg gtccaaaac 
      actcggtcc gttctggaa gacgtgcag aaggaagtc 
      gagactttg cagctgcac atcgaaggt gcgcgctac 
      accagggag tcgcaactg tacaaggac ttgaaggag 
      aagttggag gggtgcaag gagcgggtc agaaatttg 
      gatccaaat gccgacagt ttgctcaag tacattgac 
      aatgtttcc cttctcttg gagaacaat ttgtcgagt 
      cgtaaaagc acgactgag gatattctg gtggatatt 
      atgtcggtt agcacgcgg gtcaatgac ttggggggg 
      aagctgcaa ggtgtgacc aactcgctc gattttgac 
      tcgatcagg agtagttta ttggagcac caacagtac 
      ctcaggaac ttgcacatt tcggaaaac cgctcaagt 
      ctcaataaa tcgcgcgag gatattttg caacaaatc 
      gaggcatat ttgcgcaaa ctggacgag ttgagggtg 
      aaaatcgac cgcgggtcg gaggaagag aggagattt 
      aaccagagc ttgcgcaaa gttaaggag aaagtgaac 
      aggtatagt ggtacttat cggaacgtc ctgtacaac 
      gccattgag cgcgacttg gacaagcat ttgcgggac 
      gttgatcga gttttcaag gactctaac ctcgccctc 
      aaggtcaga cgcgacatt gagaactgc aaaatgatt 
      ctggagcag aaatccagc aagaataac aacgtcaag 
      cgggtggag ttggccgaa gttcggcaa aatgtcgaa 
      agcgtgaag gagaagttg accaaaatc cagcccttg 
      actgaggag gaactggcc cagcttgac gccaggctg 
      actttatcg ctcctggcc cttgacaac ttcaaggac 
      gatgattcc agtgaacgt gcccagttg tataaagag 
      ataaagttt tacggtgcc aggatccag gaacttaag 
      ttggtcccc aaaccccac tcagtgcta gtcgaagac 
      ttgcaacag aaacaactg gagctacaa caactcgct 
      gcccgaaaa atcgcccaa attgagaac gaactcgcg 
      gaaatcaag cccgaaatc gccactttt gtgggttcc 
      gagaccagt ccgaagttc ctcgcgctt gacgaggct 
      ctggttaag gccacagtg cgtctggac gagttgggg 
      atcgagcag acgagtgag ttgcaccac ttgaaggtg 
      aagctgatg cgcgagatc caggagctg gccgaggtg 
      ttggatgag agggtgaag gaggctgaa gagattgaa 
      aagtacgag aaggaggtg caggttatg ttgattaag 
      attgattcg tttaatggg tttttcggg gacgctgaa 
      tacacgtat ctggatgag gagataatt cggcttaaa 
      atcaatctg gggaagttg agggttaac caggatttg 
      gtggctagg aaggaggag tgtatgagg gagattgat 
      gcgtgtatg aggaagttg gatgaggcg gccaagacg 
      agaagtgat gcttttgag gggacgttg gtttaa
    mRNA sequence
    • >TC006768 RA
      ttgttctta tcagtcgtt atcatttta tcgatgata 
      acgcctcgt aatttccac cagctgcaa tatagtgat 
      gcttgtgct ctaaaagat gggtcaaaa tgagtcgaa 
      actcaagct gcaacaaat caccgactc tctaggtcg 
      catccgaaa ggacgtacg aagcggtaa aatcacggc 
      aaatgaggt cgaacactt ccaagcgat tttacaccg 
      atattcgga cgaaatcgc cgcaaatcc aggccgtaa 
      agcacgcac tagtggcgt ttacacagc cgaacaact 
      tatcgaaat cacctccaa agagctgga cgccctcaa 
      acaagagga ccaaggcgc caccaactc actcacacg 
      aacttcgtc caaaaaata caccaaaga caaggaaaa 
      agacgtcaa atcaatcat agcctcagt ggagcaaat 
      ccgcgagtt ggtgcgaaa tggccaagt ccaagccca 
      agattttga cagttacca ggcccgtct ggccgatta 
      ttcacacag gcttagctt catttcgaa aaaatccaa 
      aaaagagga agtcaattc ggcccaaaa actggcttc 
      tttggccac caccgagtt gttgcaact gcaggagga 
      aaaaaatga caaagttgc aacaccacc atcacaacg 
      gtcgacaat gaggcgcaa aattgccaa cagacacag 
      tcctggcaa tttggagga cattgaaac caaactgga 
      ggctctgaa gagtgacgt taaagtggc cgaaacgaa 
      tcgggacaa aaccgtgct acatttctt gtccaagtc 
      gatccaaac cctaatgac tgacttgca aatgatcaa 
      ggtccagga cgatataat cacggatga gagaaaact 
      acttttgga gaagaattt gattcgttt gaaccaaca 
      aatcgacaa gctcaagcg gcagttgaa cactgaaag 
      taacatcag ttatttagc cgagttaca acaaaaaga 
      caagcggga gaagttgct caaggactt ggacgacat 
      cgaaaagtc tgttctggc aatcagtcg taacctatc 
      gagtgtcaa tttcgaatc agagaagga aagttttaa 
      caaatacaa agcccaact gagcacaat cgaggaact 
      tagccagga aatcagtct gaagaagcg ccaagtctc 
      ggacgtgat cctcaacat tgagaacaa cttcacccg 
      cttggagat ggaggagcg cttgaacca ggccgagtt 
      caagcactt ccagacccg aattgacct tgacaattt 
      caccgcaac ctccgatag tgacttgta caaaaaaaa 
      gcgccagtt tttggtcca gttgctgga ccacgtcgc 
      tggaattat gatcaccgg ggacaagaa aaactacgt 
      cttgtacaa catcgagaa taatattaa ggcacttga 
      ctggaaagt gtcccaaaa cgacgaaaa actgaaccg 
      ggacgaaga aaaggacga gtttgtggt tttgcgccc 
      caacttgct aaaagtctg ccgcgagat tccagccag 
      gggggaaat gtccgagat tttggccga agctagggc 
      cataattga gcaaaagtt gcaaataat tgcgggggc 
      aagcgccca gctggtgac agtcaagga ggatttcct 
      ccaaccggt ccaaaacac tcggtccgt tctggaaga 
      cgtgcagaa ggaagtcga gactttgca gctgcacat 
      cgaaggtgc gcgctacac cagggagtc gcaactgta 
      caaggactt gaaggagaa gttggaggg gtgcaagga 
      gcgggtcag aaatttgga tccaaatgc cgacagttt 
      gctcaagta cattgacaa tgtttccct tctcttgga 
      gaacaattt gtcgagtcg taaaagcac gactgagga 
      tattctggt ggatattat gtcggttag cacgcgggt 
      caatgactt gggggggaa gctgcaagg tgtgaccaa 
      ctcgctcga ttttgactc gatcaggag tagtttatt 
      ggagcacca acagtacct caggaactt gcacatttc 
      ggaaaaccg ctcaagtct caataaatc gcgcgagga 
      tattttgca acaaatcga ggcatattt gcgcaaact 
      ggacgagtt gagggtgaa aatcgaccg cgggtcgga 
      ggaagagag gagatttaa ccagagctt gcgcaaagt 
      taaggagaa agtgaacag gtatagtgg tacttatcg 
      gaacgtcct gtacaacgc cattgagcg cgacttgga 
      caagcattt gcgggacgt tgatcgagt tttcaagga 
      ctctaacct cgccctcaa ggtcagacg cgacattga 
      gaactgcaa aatgattct ggagcagaa atccagcaa 
      gaataacaa cgtcaagcg ggtggagtt ggccgaagt 
      tcggcaaaa tgtcgaaag cgtgaagga gaagttgac 
      caaaatcca gcccttgac tgaggagga actggccca 
      gcttgacgc caggctgac tttatcgct cctggccct 
      tgacaactt caaggacga tgattccag tgaacgtgc 
      ccagttgta taaagagat aaagtttta cggtgccag 
      gatccagga acttaagtt ggtccccaa accccactc 
      agtgctagt cgaagactt gcaacagaa acaactgga 
      gctacaaca actcgctgc ccgaaaaat cgcccaaat 
      tgagaacga actcgcgga aatcaagcc cgaaatcgc 
      cacttttgt gggttccga gaccagtcc gaagttcct 
      cgcgcttga cgaggctct ggttaaggc cacagtgcg 
      tctggacga gttggggat cgagcagac gagtgagtt 
      gcaccactt gaaggtgaa gctgatgcg cgagatcca 
      ggagctggc cgaggtgtt ggatgagag ggtgaagga 
      ggctgaaga gattgaaaa gtacgagaa ggaggtgca 
      ggttatgtt gattaagat tgattcgtt taatgggtt 
      tttcgggga cgctgaata cacgtatct ggatgagga 
      gataattcg gcttaaaat caatctggg gaagttgag 
      ggttaacca ggatttggt ggctaggaa ggaggagtg 
      tatgaggga gattgatgc gtgtatgag gaagttgga 
      tgaggcggc caagacgag aagtgatgc ttttgaggg 
      gacgttggt ttaatttat tgtaaatgt tttaaataa 
      agttattat taattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY