| General information | |||
| TC006768 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13914105..13917332 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006768 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01089 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 2
- >TC006768 RA
MGQNESKLK LQQITDSLG RIRKDVRSG KITANEVEH FQAILHRYS DEIAANPGR KARTSGVYT AEQLIEITS KELDALKQE DQGATNSLT RTSSKKYTK DKEKDVKSI IASVEQIRE LVRNGQVQA QDFDSYQAR LADYSHRLS FISKKSKKE EVNSAQKLA SLATTELLQ LQEEKNDKV ATPPSQRST MRRKIANRH SPGNLEDIE TKLEALKSD VKVAETNRD KTVLHFLSK SIQTLMTDL QMIKVQDDI ITDERKLLL EKNLIRLNQ QIDKLKRQL NTESNISYL AELQQKDKR EKLLKDLDD IEKSVLAIS RNLSSVNFE SEKESFNKY KAQLSTIEE LSQEISLKK RQVSDVILN IENNFTRLE MEERLNQAE FKHFQTRID LDNFTATSD SDLYKKKRQ FLVQLLDHV AGIMITGDK KNYVLYNIE NNIKALDWK VSQNDEKLN RDEEKDEFV VLRPNLLKV CREIPARGE MSEILAEAR AIIEQKLQI IAGASAQLV TVKEDFLQP VQNTRSVLE DVQKEVETL QLHIEGARY TRESQLYKD LKEKLEGCK ERVRNLDPN ADSLLKYID NVSLLLENN LSSRKSTTE DILVDIMSV STRVNDLGG KLQGVTNSL DFDSIRSSL LEHQQYLRN LHISENRSS LNKSREDIL QQIEAYLRK LDELRVKID RGSEEERRF NQSLRKVKE KVNRYSGTY RNVLYNAIE RDLDKHLRD VDRVFKDSN LALKVRRDI ENCKMILEQ KSSKNNNVK RVELAEVRQ NVESVKEKL TKIQPLTEE ELAQLDARL TLSLLALDN FKDDDSSER AQLYKEIKF YGARIQELK LVPKPHSVL VEDLQQKQL ELQQLAARK IAQIENELA EIKPEIATF VGSETSPKF LALDEALVK ATVRLDELG IEQTSELHH LKVKLMREI QELAEVLDE RVKEAEEIE KYEKEVQVM LIKIDSFNG FFGDAEYTY LDEEIIRLK INLGKLRVN QDLVARKEE CMREIDACM RKLDEAAKT RSDAFEGTL V
- >TC006768 RA
atgggtcaa aatgagtcg aaactcaag ctgcaacaa atcaccgac tctctaggt cgcatccga aaggacgta cgaagcggt aaaatcacg gcaaatgag gtcgaacac ttccaagcg attttacac cgatattcg gacgaaatc gccgcaaat ccaggccgt aaagcacgc actagtggc gtttacaca gccgaacaa cttatcgaa atcacctcc aaagagctg gacgccctc aaacaagag gaccaaggc gccaccaac tcactcaca cgaacttcg tccaaaaaa tacaccaaa gacaaggaa aaagacgtc aaatcaatc atagcctca gtggagcaa atccgcgag ttggtgcga aatggccaa gtccaagcc caagatttt gacagttac caggcccgt ctggccgat tattcacac aggcttagc ttcatttcg aaaaaatcc aaaaaagag gaagtcaat tcggcccaa aaactggct tctttggcc accaccgag ttgttgcaa ctgcaggag gaaaaaaat gacaaagtt gcaacacca ccatcacaa cggtcgaca atgaggcgc aaaattgcc aacagacac agtcctggc aatttggag gacattgaa accaaactg gaggctctg aagagtgac gttaaagtg gccgaaacg aatcgggac aaaaccgtg ctacatttc ttgtccaag tcgatccaa accctaatg actgacttg caaatgatc aaggtccag gacgatata atcacggat gagagaaaa ctacttttg gagaagaat ttgattcgt ttgaaccaa caaatcgac aagctcaag cggcagttg aacactgaa agtaacatc agttattta gccgagtta caacaaaaa gacaagcgg gagaagttg ctcaaggac ttggacgac atcgaaaag tctgttctg gcaatcagt cgtaaccta tcgagtgtc aatttcgaa tcagagaag gaaagtttt aacaaatac aaagcccaa ctgagcaca atcgaggaa cttagccag gaaatcagt ctgaagaag cgccaagtc tcggacgtg atcctcaac attgagaac aacttcacc cgcttggag atggaggag cgcttgaac caggccgag ttcaagcac ttccagacc cgaattgac cttgacaat ttcaccgca acctccgat agtgacttg tacaaaaaa aagcgccag tttttggtc cagttgctg gaccacgtc gctggaatt atgatcacc ggggacaag aaaaactac gtcttgtac aacatcgag aataatatt aaggcactt gactggaaa gtgtcccaa aacgacgaa aaactgaac cgggacgaa gaaaaggac gagtttgtg gttttgcgc cccaacttg ctaaaagtc tgccgcgag attccagcc aggggggaa atgtccgag attttggcc gaagctagg gccataatt gagcaaaag ttgcaaata attgcgggg gcaagcgcc cagctggtg acagtcaag gaggatttc ctccaaccg gtccaaaac actcggtcc gttctggaa gacgtgcag aaggaagtc gagactttg cagctgcac atcgaaggt gcgcgctac accagggag tcgcaactg tacaaggac ttgaaggag aagttggag gggtgcaag gagcgggtc agaaatttg gatccaaat gccgacagt ttgctcaag tacattgac aatgtttcc cttctcttg gagaacaat ttgtcgagt cgtaaaagc acgactgag gatattctg gtggatatt atgtcggtt agcacgcgg gtcaatgac ttggggggg aagctgcaa ggtgtgacc aactcgctc gattttgac tcgatcagg agtagttta ttggagcac caacagtac ctcaggaac ttgcacatt tcggaaaac cgctcaagt ctcaataaa tcgcgcgag gatattttg caacaaatc gaggcatat ttgcgcaaa ctggacgag ttgagggtg aaaatcgac cgcgggtcg gaggaagag aggagattt aaccagagc ttgcgcaaa gttaaggag aaagtgaac aggtatagt ggtacttat cggaacgtc ctgtacaac gccattgag cgcgacttg gacaagcat ttgcgggac gttgatcga gttttcaag gactctaac ctcgccctc aaggtcaga cgcgacatt gagaactgc aaaatgatt ctggagcag aaatccagc aagaataac aacgtcaag cgggtggag ttggccgaa gttcggcaa aatgtcgaa agcgtgaag gagaagttg accaaaatc cagcccttg actgaggag gaactggcc cagcttgac gccaggctg actttatcg ctcctggcc cttgacaac ttcaaggac gatgattcc agtgaacgt gcccagttg tataaagag ataaagttt tacggtgcc aggatccag gaacttaag ttggtcccc aaaccccac tcagtgcta gtcgaagac ttgcaacag aaacaactg gagctacaa caactcgct gcccgaaaa atcgcccaa attgagaac gaactcgcg gaaatcaag cccgaaatc gccactttt gtgggttcc gagaccagt ccgaagttc ctcgcgctt gacgaggct ctggttaag gccacagtg cgtctggac gagttgggg atcgagcag acgagtgag ttgcaccac ttgaaggtg aagctgatg cgcgagatc caggagctg gccgaggtg ttggatgag agggtgaag gaggctgaa gagattgaa aagtacgag aaggaggtg caggttatg ttgattaag attgattcg tttaatggg tttttcggg gacgctgaa tacacgtat ctggatgag gagataatt cggcttaaa atcaatctg gggaagttg agggttaac caggatttg gtggctagg aaggaggag tgtatgagg gagattgat gcgtgtatg aggaagttg gatgaggcg gccaagacg agaagtgat gcttttgag gggacgttg gtttaa
- >TC006768 RA
ttgttctta tcagtcgtt atcatttta tcgatgata acgcctcgt aatttccac cagctgcaa tatagtgat gcttgtgct ctaaaagat gggtcaaaa tgagtcgaa actcaagct gcaacaaat caccgactc tctaggtcg catccgaaa ggacgtacg aagcggtaa aatcacggc aaatgaggt cgaacactt ccaagcgat tttacaccg atattcgga cgaaatcgc cgcaaatcc aggccgtaa agcacgcac tagtggcgt ttacacagc cgaacaact tatcgaaat cacctccaa agagctgga cgccctcaa acaagagga ccaaggcgc caccaactc actcacacg aacttcgtc caaaaaata caccaaaga caaggaaaa agacgtcaa atcaatcat agcctcagt ggagcaaat ccgcgagtt ggtgcgaaa tggccaagt ccaagccca agattttga cagttacca ggcccgtct ggccgatta ttcacacag gcttagctt catttcgaa aaaatccaa aaaagagga agtcaattc ggcccaaaa actggcttc tttggccac caccgagtt gttgcaact gcaggagga aaaaaatga caaagttgc aacaccacc atcacaacg gtcgacaat gaggcgcaa aattgccaa cagacacag tcctggcaa tttggagga cattgaaac caaactgga ggctctgaa gagtgacgt taaagtggc cgaaacgaa tcgggacaa aaccgtgct acatttctt gtccaagtc gatccaaac cctaatgac tgacttgca aatgatcaa ggtccagga cgatataat cacggatga gagaaaact acttttgga gaagaattt gattcgttt gaaccaaca aatcgacaa gctcaagcg gcagttgaa cactgaaag taacatcag ttatttagc cgagttaca acaaaaaga caagcggga gaagttgct caaggactt ggacgacat cgaaaagtc tgttctggc aatcagtcg taacctatc gagtgtcaa tttcgaatc agagaagga aagttttaa caaatacaa agcccaact gagcacaat cgaggaact tagccagga aatcagtct gaagaagcg ccaagtctc ggacgtgat cctcaacat tgagaacaa cttcacccg cttggagat ggaggagcg cttgaacca ggccgagtt caagcactt ccagacccg aattgacct tgacaattt caccgcaac ctccgatag tgacttgta caaaaaaaa gcgccagtt tttggtcca gttgctgga ccacgtcgc tggaattat gatcaccgg ggacaagaa aaactacgt cttgtacaa catcgagaa taatattaa ggcacttga ctggaaagt gtcccaaaa cgacgaaaa actgaaccg ggacgaaga aaaggacga gtttgtggt tttgcgccc caacttgct aaaagtctg ccgcgagat tccagccag gggggaaat gtccgagat tttggccga agctagggc cataattga gcaaaagtt gcaaataat tgcgggggc aagcgccca gctggtgac agtcaagga ggatttcct ccaaccggt ccaaaacac tcggtccgt tctggaaga cgtgcagaa ggaagtcga gactttgca gctgcacat cgaaggtgc gcgctacac cagggagtc gcaactgta caaggactt gaaggagaa gttggaggg gtgcaagga gcgggtcag aaatttgga tccaaatgc cgacagttt gctcaagta cattgacaa tgtttccct tctcttgga gaacaattt gtcgagtcg taaaagcac gactgagga tattctggt ggatattat gtcggttag cacgcgggt caatgactt gggggggaa gctgcaagg tgtgaccaa ctcgctcga ttttgactc gatcaggag tagtttatt ggagcacca acagtacct caggaactt gcacatttc ggaaaaccg ctcaagtct caataaatc gcgcgagga tattttgca acaaatcga ggcatattt gcgcaaact ggacgagtt gagggtgaa aatcgaccg cgggtcgga ggaagagag gagatttaa ccagagctt gcgcaaagt taaggagaa agtgaacag gtatagtgg tacttatcg gaacgtcct gtacaacgc cattgagcg cgacttgga caagcattt gcgggacgt tgatcgagt tttcaagga ctctaacct cgccctcaa ggtcagacg cgacattga gaactgcaa aatgattct ggagcagaa atccagcaa gaataacaa cgtcaagcg ggtggagtt ggccgaagt tcggcaaaa tgtcgaaag cgtgaagga gaagttgac caaaatcca gcccttgac tgaggagga actggccca gcttgacgc caggctgac tttatcgct cctggccct tgacaactt caaggacga tgattccag tgaacgtgc ccagttgta taaagagat aaagtttta cggtgccag gatccagga acttaagtt ggtccccaa accccactc agtgctagt cgaagactt gcaacagaa acaactgga gctacaaca actcgctgc ccgaaaaat cgcccaaat tgagaacga actcgcgga aatcaagcc cgaaatcgc cacttttgt gggttccga gaccagtcc gaagttcct cgcgcttga cgaggctct ggttaaggc cacagtgcg tctggacga gttggggat cgagcagac gagtgagtt gcaccactt gaaggtgaa gctgatgcg cgagatcca ggagctggc cgaggtgtt ggatgagag ggtgaagga ggctgaaga gattgaaaa gtacgagaa ggaggtgca ggttatgtt gattaagat tgattcgtt taatgggtt tttcgggga cgctgaata cacgtatct ggatgagga gataattcg gcttaaaat caatctggg gaagttgag ggttaacca ggatttggt ggctaggaa ggaggagtg tatgaggga gattgatgc gtgtatgag gaagttgga tgaggcggc caagacgag aagtgatgc ttttgaggg gacgttggt ttaatttat tgtaaatgt tttaaataa agttattat taattg
