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Gene information
General information
TC006768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13914105..13917332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006768
    iBeetle screen iB_01089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006768 RA
      MGQNESKLK LQQITDSLG RIRKDVRSG KITANEVEH 
FQAILHRYS DEIAANPGR KARTSGVYT AEQLIEITS 
KELDALKQE DQGATNSLT RTSSKKYTK DKEKDVKSI 
IASVEQIRE LVRNGQVQA QDFDSYQAR LADYSHRLS 
FISKKSKKE EVNSAQKLA SLATTELLQ LQEEKNDKV 
ATPPSQRST MRRKIANRH SPGNLEDIE TKLEALKSD 
VKVAETNRD KTVLHFLSK SIQTLMTDL QMIKVQDDI 
ITDERKLLL EKNLIRLNQ QIDKLKRQL NTESNISYL 
AELQQKDKR EKLLKDLDD IEKSVLAIS RNLSSVNFE 
SEKESFNKY KAQLSTIEE LSQEISLKK RQVSDVILN 
IENNFTRLE MEERLNQAE FKHFQTRID LDNFTATSD 
SDLYKKKRQ FLVQLLDHV AGIMITGDK KNYVLYNIE 
NNIKALDWK VSQNDEKLN RDEEKDEFV VLRPNLLKV 
CREIPARGE MSEILAEAR AIIEQKLQI IAGASAQLV 
TVKEDFLQP VQNTRSVLE DVQKEVETL QLHIEGARY 
TRESQLYKD LKEKLEGCK ERVRNLDPN ADSLLKYID 
NVSLLLENN LSSRKSTTE DILVDIMSV STRVNDLGG 
KLQGVTNSL DFDSIRSSL LEHQQYLRN LHISENRSS 
LNKSREDIL QQIEAYLRK LDELRVKID RGSEEERRF 
NQSLRKVKE KVNRYSGTY RNVLYNAIE RDLDKHLRD 
VDRVFKDSN LALKVRRDI ENCKMILEQ KSSKNNNVK 
RVELAEVRQ NVESVKEKL TKIQPLTEE ELAQLDARL 
TLSLLALDN FKDDDSSER AQLYKEIKF YGARIQELK 
LVPKPHSVL VEDLQQKQL ELQQLAARK IAQIENELA 
EIKPEIATF VGSETSPKF LALDEALVK ATVRLDELG 
IEQTSELHH LKVKLMREI QELAEVLDE RVKEAEEIE 
KYEKEVQVM LIKIDSFNG FFGDAEYTY LDEEIIRLK 
INLGKLRVN QDLVARKEE CMREIDACM RKLDEAAKT 
RSDAFEGTL V
    CDS sequence
    • >TC006768 RA
      atgggtcaa aatgagtcg aaactcaag ctgcaacaa 
atcaccgac tctctaggt cgcatccga aaggacgta 
cgaagcggt aaaatcacg gcaaatgag gtcgaacac 
ttccaagcg attttacac cgatattcg gacgaaatc 
gccgcaaat ccaggccgt aaagcacgc actagtggc 
gtttacaca gccgaacaa cttatcgaa atcacctcc 
aaagagctg gacgccctc aaacaagag gaccaaggc 
gccaccaac tcactcaca cgaacttcg tccaaaaaa 
tacaccaaa gacaaggaa aaagacgtc aaatcaatc 
atagcctca gtggagcaa atccgcgag ttggtgcga 
aatggccaa gtccaagcc caagatttt gacagttac 
caggcccgt ctggccgat tattcacac aggcttagc 
ttcatttcg aaaaaatcc aaaaaagag gaagtcaat 
tcggcccaa aaactggct tctttggcc accaccgag 
ttgttgcaa ctgcaggag gaaaaaaat gacaaagtt 
gcaacacca ccatcacaa cggtcgaca atgaggcgc 
aaaattgcc aacagacac agtcctggc aatttggag 
gacattgaa accaaactg gaggctctg aagagtgac 
gttaaagtg gccgaaacg aatcgggac aaaaccgtg 
ctacatttc ttgtccaag tcgatccaa accctaatg 
actgacttg caaatgatc aaggtccag gacgatata 
atcacggat gagagaaaa ctacttttg gagaagaat 
ttgattcgt ttgaaccaa caaatcgac aagctcaag 
cggcagttg aacactgaa agtaacatc agttattta 
gccgagtta caacaaaaa gacaagcgg gagaagttg 
ctcaaggac ttggacgac atcgaaaag tctgttctg 
gcaatcagt cgtaaccta tcgagtgtc aatttcgaa 
tcagagaag gaaagtttt aacaaatac aaagcccaa 
ctgagcaca atcgaggaa cttagccag gaaatcagt 
ctgaagaag cgccaagtc tcggacgtg atcctcaac 
attgagaac aacttcacc cgcttggag atggaggag 
cgcttgaac caggccgag ttcaagcac ttccagacc 
cgaattgac cttgacaat ttcaccgca acctccgat 
agtgacttg tacaaaaaa aagcgccag tttttggtc 
cagttgctg gaccacgtc gctggaatt atgatcacc 
ggggacaag aaaaactac gtcttgtac aacatcgag 
aataatatt aaggcactt gactggaaa gtgtcccaa 
aacgacgaa aaactgaac cgggacgaa gaaaaggac 
gagtttgtg gttttgcgc cccaacttg ctaaaagtc 
tgccgcgag attccagcc aggggggaa atgtccgag 
attttggcc gaagctagg gccataatt gagcaaaag 
ttgcaaata attgcgggg gcaagcgcc cagctggtg 
acagtcaag gaggatttc ctccaaccg gtccaaaac 
actcggtcc gttctggaa gacgtgcag aaggaagtc 
gagactttg cagctgcac atcgaaggt gcgcgctac 
accagggag tcgcaactg tacaaggac ttgaaggag 
aagttggag gggtgcaag gagcgggtc agaaatttg 
gatccaaat gccgacagt ttgctcaag tacattgac 
aatgtttcc cttctcttg gagaacaat ttgtcgagt 
cgtaaaagc acgactgag gatattctg gtggatatt 
atgtcggtt agcacgcgg gtcaatgac ttggggggg 
aagctgcaa ggtgtgacc aactcgctc gattttgac 
tcgatcagg agtagttta ttggagcac caacagtac 
ctcaggaac ttgcacatt tcggaaaac cgctcaagt 
ctcaataaa tcgcgcgag gatattttg caacaaatc 
gaggcatat ttgcgcaaa ctggacgag ttgagggtg 
aaaatcgac cgcgggtcg gaggaagag aggagattt 
aaccagagc ttgcgcaaa gttaaggag aaagtgaac 
aggtatagt ggtacttat cggaacgtc ctgtacaac 
gccattgag cgcgacttg gacaagcat ttgcgggac 
gttgatcga gttttcaag gactctaac ctcgccctc 
aaggtcaga cgcgacatt gagaactgc aaaatgatt 
ctggagcag aaatccagc aagaataac aacgtcaag 
cgggtggag ttggccgaa gttcggcaa aatgtcgaa 
agcgtgaag gagaagttg accaaaatc cagcccttg 
actgaggag gaactggcc cagcttgac gccaggctg 
actttatcg ctcctggcc cttgacaac ttcaaggac 
gatgattcc agtgaacgt gcccagttg tataaagag 
ataaagttt tacggtgcc aggatccag gaacttaag 
ttggtcccc aaaccccac tcagtgcta gtcgaagac 
ttgcaacag aaacaactg gagctacaa caactcgct 
gcccgaaaa atcgcccaa attgagaac gaactcgcg 
gaaatcaag cccgaaatc gccactttt gtgggttcc 
gagaccagt ccgaagttc ctcgcgctt gacgaggct 
ctggttaag gccacagtg cgtctggac gagttgggg 
atcgagcag acgagtgag ttgcaccac ttgaaggtg 
aagctgatg cgcgagatc caggagctg gccgaggtg 
ttggatgag agggtgaag gaggctgaa gagattgaa 
aagtacgag aaggaggtg caggttatg ttgattaag 
attgattcg tttaatggg tttttcggg gacgctgaa 
tacacgtat ctggatgag gagataatt cggcttaaa 
atcaatctg gggaagttg agggttaac caggatttg 
gtggctagg aaggaggag tgtatgagg gagattgat 
gcgtgtatg aggaagttg gatgaggcg gccaagacg 
agaagtgat gcttttgag gggacgttg gtttaa
    mRNA sequence
    • >TC006768 RA
      ttgttctta tcagtcgtt atcatttta tcgatgata 
acgcctcgt aatttccac cagctgcaa tatagtgat 
gcttgtgct ctaaaagat gggtcaaaa tgagtcgaa 
actcaagct gcaacaaat caccgactc tctaggtcg 
catccgaaa ggacgtacg aagcggtaa aatcacggc 
aaatgaggt cgaacactt ccaagcgat tttacaccg 
atattcgga cgaaatcgc cgcaaatcc aggccgtaa 
agcacgcac tagtggcgt ttacacagc cgaacaact 
tatcgaaat cacctccaa agagctgga cgccctcaa 
acaagagga ccaaggcgc caccaactc actcacacg 
aacttcgtc caaaaaata caccaaaga caaggaaaa 
agacgtcaa atcaatcat agcctcagt ggagcaaat 
ccgcgagtt ggtgcgaaa tggccaagt ccaagccca 
agattttga cagttacca ggcccgtct ggccgatta 
ttcacacag gcttagctt catttcgaa aaaatccaa 
aaaagagga agtcaattc ggcccaaaa actggcttc 
tttggccac caccgagtt gttgcaact gcaggagga 
aaaaaatga caaagttgc aacaccacc atcacaacg 
gtcgacaat gaggcgcaa aattgccaa cagacacag 
tcctggcaa tttggagga cattgaaac caaactgga 
ggctctgaa gagtgacgt taaagtggc cgaaacgaa 
tcgggacaa aaccgtgct acatttctt gtccaagtc 
gatccaaac cctaatgac tgacttgca aatgatcaa 
ggtccagga cgatataat cacggatga gagaaaact 
acttttgga gaagaattt gattcgttt gaaccaaca 
aatcgacaa gctcaagcg gcagttgaa cactgaaag 
taacatcag ttatttagc cgagttaca acaaaaaga 
caagcggga gaagttgct caaggactt ggacgacat 
cgaaaagtc tgttctggc aatcagtcg taacctatc 
gagtgtcaa tttcgaatc agagaagga aagttttaa 
caaatacaa agcccaact gagcacaat cgaggaact 
tagccagga aatcagtct gaagaagcg ccaagtctc 
ggacgtgat cctcaacat tgagaacaa cttcacccg 
cttggagat ggaggagcg cttgaacca ggccgagtt 
caagcactt ccagacccg aattgacct tgacaattt 
caccgcaac ctccgatag tgacttgta caaaaaaaa 
gcgccagtt tttggtcca gttgctgga ccacgtcgc 
tggaattat gatcaccgg ggacaagaa aaactacgt 
cttgtacaa catcgagaa taatattaa ggcacttga 
ctggaaagt gtcccaaaa cgacgaaaa actgaaccg 
ggacgaaga aaaggacga gtttgtggt tttgcgccc 
caacttgct aaaagtctg ccgcgagat tccagccag 
gggggaaat gtccgagat tttggccga agctagggc 
cataattga gcaaaagtt gcaaataat tgcgggggc 
aagcgccca gctggtgac agtcaagga ggatttcct 
ccaaccggt ccaaaacac tcggtccgt tctggaaga 
cgtgcagaa ggaagtcga gactttgca gctgcacat 
cgaaggtgc gcgctacac cagggagtc gcaactgta 
caaggactt gaaggagaa gttggaggg gtgcaagga 
gcgggtcag aaatttgga tccaaatgc cgacagttt 
gctcaagta cattgacaa tgtttccct tctcttgga 
gaacaattt gtcgagtcg taaaagcac gactgagga 
tattctggt ggatattat gtcggttag cacgcgggt 
caatgactt gggggggaa gctgcaagg tgtgaccaa 
ctcgctcga ttttgactc gatcaggag tagtttatt 
ggagcacca acagtacct caggaactt gcacatttc 
ggaaaaccg ctcaagtct caataaatc gcgcgagga 
tattttgca acaaatcga ggcatattt gcgcaaact 
ggacgagtt gagggtgaa aatcgaccg cgggtcgga 
ggaagagag gagatttaa ccagagctt gcgcaaagt 
taaggagaa agtgaacag gtatagtgg tacttatcg 
gaacgtcct gtacaacgc cattgagcg cgacttgga 
caagcattt gcgggacgt tgatcgagt tttcaagga 
ctctaacct cgccctcaa ggtcagacg cgacattga 
gaactgcaa aatgattct ggagcagaa atccagcaa 
gaataacaa cgtcaagcg ggtggagtt ggccgaagt 
tcggcaaaa tgtcgaaag cgtgaagga gaagttgac 
caaaatcca gcccttgac tgaggagga actggccca 
gcttgacgc caggctgac tttatcgct cctggccct 
tgacaactt caaggacga tgattccag tgaacgtgc 
ccagttgta taaagagat aaagtttta cggtgccag 
gatccagga acttaagtt ggtccccaa accccactc 
agtgctagt cgaagactt gcaacagaa acaactgga 
gctacaaca actcgctgc ccgaaaaat cgcccaaat 
tgagaacga actcgcgga aatcaagcc cgaaatcgc 
cacttttgt gggttccga gaccagtcc gaagttcct 
cgcgcttga cgaggctct ggttaaggc cacagtgcg 
tctggacga gttggggat cgagcagac gagtgagtt 
gcaccactt gaaggtgaa gctgatgcg cgagatcca 
ggagctggc cgaggtgtt ggatgagag ggtgaagga 
ggctgaaga gattgaaaa gtacgagaa ggaggtgca 
ggttatgtt gattaagat tgattcgtt taatgggtt 
tttcgggga cgctgaata cacgtatct ggatgagga 
gataattcg gcttaaaat caatctggg gaagttgag 
ggttaacca ggatttggt ggctaggaa ggaggagtg 
tatgaggga gattgatgc gtgtatgag gaagttgga 
tgaggcggc caagacgag aagtgatgc ttttgaggg 
gacgttggt ttaatttat tgtaaatgt tttaaataa 
agttattat taattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY