Status
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Gene information
General information
TC006758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13801169..13804433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006758
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01088

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      antenna sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006758 RA
        MSPPFVERE LCVENLDEE PPLQQELTF GRSYRVWQQ 
        VEDTSPIPP KPSPINVPF ELCRAVQKP HKKKKSKLC 
        KCSLRPFCQ SDALDPKMP LLDRSALPQ SKYMDMLSI 
        PHGRKPPCK KKKYKASVC CKCQVEPCP PRIIQMSVP 
        NKRYVLQNW KDYQHILPA EMLVRFDQI LKSGDNLEP 
        RDARYYFKK LRRQKIKEM RKRKKLCRK KKEQKQKGE 
        LLWIKNQIR ETVDAIVDY IKNEPLFML NFQQMMTSN 
        ELLNYLANE RVVKIRSRN TKCVYKKTV IEISDKLVK 
        WMDTMNFFA DIQPLDSKE RLLEVPSLE AMLEEESEE 
        EEISEPGYE DEGYDEDEF YGAAVAGGD AGQEEFMAK 
        KLVDKTKLF DLDEEFEEG SVIDMLRGL DEDTLAKLI 
        QILSQSPED ILSAEIDPE NLPSVTYAD ILEKLKEIR 
        DGNLLEKAR KKLDLEEKM IEWARRNDP DQVDDMILD 
        KIHETSGIL GELFKNMDV SDFKKTMKE QDEDVGREI 
        IEEVMEKRQ DRGQVEATV FRESDEEGE EFREALGGE 
        EEGEPGAPP VGAVGEGEE RFIEGEEAG VPGGPGTAP 
        GLEGTASEA EGAEEAVPG TEGTAPGAE GIVPGDEGI 
        VPGDEGTVP GAEGTIPGG EEAGLGGPG AAGFGEGFE 
        GEEAAFGRD YGQGEEDAF GGAVAEGAS LGFGEGEGG 
        FGEEGEVGF GPAEVEAAE EYGRAEEAY FYGAQEDLY 
        GEAEDLLYG EEGFDEGEG YGLPPGVEL GEEGEELIM 
        GRLADELER LDSLLGVIR GHKPSVVYE HTPGTICCL 
        SLKIWAVWL LEIANNAHN WTKWIGAII KQVRHYVAI 
        IRGDVLLPS GEKQVLYKE EWRKFVKDT EEKVVAWRQ 
        YSKHVKDLS TEIMENFHG KKVNCCPKC LQDHLIKNV 
        VTAHETLQA LTEAINCAG YWQRCLDRI VEQTSKITE 
        MELGPESGA PDVTSLGSE GSYSIEIEE LAHMPTRKV 
        ASEGSYGSM YEIEELPVP GPVYSNYDI VEVLPTKPE 
        PSYFHEITE IATLEPPPK PQKVEAPKP AKPHKSNLK 
        GKKKK
      CDS sequence
      • >TC006758 RA
        atgagcccc ccgtttgtc gagcgcgag ctctgcgtg 
        gagaacctg gacgaggag ccccctttg caacaagag 
        ttgactttc gggcgctcc taccgcgtc tggcagcaa 
        gtcgaagac acctctccc atacccccc aagccctcc 
        cccatcaac gtccccttc gagttgtgc cgggccgtc 
        cagaagccc cacaagaag aaaaaaagc aagttgtgc 
        aagtgctcg ctgcggccc ttctgccaa agcgatgcg 
        ttggacccc aaaatgccc cttttggac cgcagcgct 
        ttgccccaa agcaagtac atggacatg ctgtcgata 
        ccgcatggg cgaaagccc ccttgcaaa aagaagaag 
        tataaagct agtgtttgc tgcaagtgt caagtggag 
        ccctgtccc cccaggatt attcaaatg tcggtgccc 
        aataagcgc tatgttttg caaaactgg aaggattat 
        cagcacata cttccggcg gaaatgttg gtcaggttt 
        gaccagatt ctgaaaagt ggggacaat ttggagccc 
        cgtgacgcc aggtattac ttcaagaag ttgcgaagg 
        cagaaaatt aaggaaatg aggaaacgc aaaaagttg 
        tgtcggaag aagaaggaa caaaaacaa aagggtgaa 
        ttgttatgg atcaaaaac cagattagg gaaaccgtt 
        gacgcaatc gttgattat ataaaaaat gagcccctt 
        tttatgctc aacttccaa caaatgatg acctcgaat 
        gagctcctc aactatttg gctaatgag cgagtcgtg 
        aaaatacgc tcaaggaac accaagtgt gtttacaag 
        aagactgtg attgaaatt agtgacaag ttggtcaaa 
        tggatggac accatgaac ttcttcgcc gatatacag 
        ccccttgat tcaaaggag cgccttctg gaagtgcca 
        tcacttgaa gcaatgctt gaggaagag tccgaagaa 
        gaggaaata agtgagcct ggttacgaa gatgaaggt 
        tatgacgaa gatgagttc tatggtgct gcagttgcc 
        ggaggtgac gctggacag gaagaattc atggcgaag 
        aaactcgtt gataagacc aaactcttc gatctagat 
        gaagaattt gaagaaggc tcagttatt gatatgttg 
        cgaggacta gacgaagat actttggcc aaactgatt 
        cagatcttg tcacaaagt cctgaagat attctaagt 
        gctgagata gatccagag aatcttccc agtgtcaca 
        tacgccgac attttagaa aagttgaaa gaaatccgt 
        gatgggaat ttgttggaa aaggcaagg aagaaacta 
        gatttggaa gagaaaatg atcgaatgg gcgagaaga 
        aatgatccg gatcaagtc gatgacatg attcttgat 
        aaaatacac gaaacgtcg gggatcttg ggcgagttg 
        ttcaaaaac atggacgtg agcgatttt aagaagacc 
        atgaaagaa caagatgaa gatgttggg agagaaata 
        attgaagaa gtgatggag aaacgtcag gatagagga 
        caagtcgaa gctacagtt ttcagagaa agtgacgaa 
        gaaggcgag gaatttaga gaggctttg ggtggcgaa 
        gaagaaggc gaacctgga gcacctcct gtaggagcg 
        gttggagaa ggagaagag cggtttatt gagggtgaa 
        gaagcagga gttcctgga ggtcccgga acagctcca 
        ggactcgaa ggaacagct tccgaagct gaaggagct 
        gaagaagca gttcctgga accgaagga acagctcct 
        ggagctgaa ggaatagtt cctggagat gaaggaata 
        gttcctgga gatgaagga acagttcct ggtgctgaa 
        ggaacaatt cctggaggt gaagaagca ggtcttggg 
        ggtcctgga gcggcagga ttcggggag ggtttcgag 
        ggagaagag gccgctttc gggcgggat tacggccaa 
        ggtgaagaa gatgcattt ggaggtgca gtggcagaa 
        ggtgcctca cttggattt ggagaaggt gaaggtggg 
        tttggcgaa gagggagaa gttggtttt ggccctgcc 
        gaagtagaa gcagcagaa gagtatggt cgtgcagaa 
        gaagcttat ttttacgga gcgcaagaa gatttgtac 
        ggcgaagct gaagatttg ctctatggt gaagaaggt 
        ttcgacgaa ggagaaggt tatggtcta cctcctggt 
        gtagaatta ggcgaagaa ggtgaagaa ctaattatg 
        ggtcgcttg gccgatgag cttgagcgt cttgacagc 
        ctcctgggt gtaattcgg ggacacaaa ccatccgtg 
        gtttatgaa cacactcca ggcaccatt tgttgtctc 
        tcattgaaa atttgggcc gtttggttg ttagaaatc 
        gccaataat gcccataat tggacgaaa tggattggg 
        gcaataatt aaacaagtc cgccactat gtggctata 
        atcagagga gatgtttta ctcccaagt ggcgaaaag 
        caagtcttg tacaaggag gagtggcgc aaatttgtc 
        aaagacact gaagaaaaa gttgtcgcg tggcgacaa 
        tacagcaaa catgttaaa gacttatcg accgaaatc 
        atggaaaac ttccacggt aaaaaagtt aattgttgt 
        ccgaaatgt ctccaagac catttaatt aagaacgtg 
        gtgacggca catgagact cttcaagcc ctcactgag 
        gctatcaac tgtgccggg tattggcag agatgtctc 
        gatagaata gttgagcaa acgagtaaa atcaccgaa 
        atggaactg ggccctgaa agtggggcc cctgatgta 
        acgtcattg ggttccgag gggtcgtat agtatcgaa 
        attgaagaa ttggcacac atgccaaca cgtaaagtg 
        gcctcagaa ggcagttac ggttccatg tacgaaatt 
        gaggagttg ccagtacca ggaccggtt tattccaac 
        tacgacatt gtcgaggtg ttgccaacg aagcccgaa 
        cccagttat tttcacgaa atcaccgaa attgcgact 
        ttggagccc ccacccaag ccgcaaaaa gtggaggca 
        cccaaaccc gccaaacca cacaaatcg aatttgaag 
        ggaaagaaa aagaaataa
      mRNA sequence
      • >TC006758 RA
        acgagcgcg caatttaaa taaaatttt caaaacagt 
        ttggtttta ttgacactt gtcaccccc taaatcccc 
        gtttcgcga ccatgagcc ccccgtttg tcgagcgcg 
        agctctgcg tggagaacc tggacgagg agccccctt 
        tgcaacaag agttgactt tcgggcgct cctaccgcg 
        tctggcagc aagtcgaag acacctctc ccatacccc 
        ccaagccct cccccatca acgtcccct tcgagttgt 
        gccgggccg tccagaagc cccacaaga agaaaaaaa 
        gcaagttgt gcaagtgct cgctgcggc ccttctgcc 
        aaagcgatg cgttggacc ccaaaatgc cccttttgg 
        accgcagcg ctttgcccc aaagcaagt acatggaca 
        tgctgtcga taccgcatg ggcgaaagc ccccttgca 
        aaaagaaga agtataaag ctagtgttt gctgcaagt 
        gtcaagtgg agccctgtc cccccagga ttattcaaa 
        tgtcggtgc ccaataagc gctatgttt tgcaaaact 
        ggaaggatt atcagcaca tacttccgg cggaaatgt 
        tggtcaggt ttgaccaga ttctgaaaa gtggggaca 
        atttggagc cccgtgacg ccaggtatt acttcaaga 
        agttgcgaa ggcagaaaa ttaaggaaa tgaggaaac 
        gcaaaaagt tgtgtcgga agaagaagg aacaaaaac 
        aaaagggtg aattgttat ggatcaaaa accagatta 
        gggaaaccg ttgacgcaa tcgttgatt atataaaaa 
        atgagcccc tttttatgc tcaacttcc aacaaatga 
        tgacctcga atgagctcc tcaactatt tggctaatg 
        agcgagtcg tgaaaatac gctcaagga acaccaagt 
        gtgtttaca agaagactg tgattgaaa ttagtgaca 
        agttggtca aatggatgg acaccatga acttcttcg 
        ccgatatac agccccttg attcaaagg agcgccttc 
        tggaagtgc catcacttg aagcaatgc ttgaggaag 
        agtccgaag aagaggaaa taagtgagc ctggttacg 
        aagatgaag gttatgacg aagatgagt tctatggtg 
        ctgcagttg ccggaggtg acgctggac aggaagaat 
        tcatggcga agaaactcg ttgataaga ccaaactct 
        tcgatctag atgaagaat ttgaagaag gctcagtta 
        ttgatatgt tgcgaggac tagacgaag atactttgg 
        ccaaactga ttcagatct tgtcacaaa gtcctgaag 
        atattctaa gtgctgaga tagatccag agaatcttc 
        ccagtgtca catacgccg acattttag aaaagttga 
        aagaaatcc gtgatggga atttgttgg aaaaggcaa 
        ggaagaaac tagatttgg aagagaaaa tgatcgaat 
        gggcgagaa gaaatgatc cggatcaag tcgatgaca 
        tgattcttg ataaaatac acgaaacgt cggggatct 
        tgggcgagt tgttcaaaa acatggacg tgagcgatt 
        ttaagaaga ccatgaaag aacaagatg aagatgttg 
        ggagagaaa taattgaag aagtgatgg agaaacgtc 
        aggatagag gacaagtcg aagctacag ttttcagag 
        aaagtgacg aagaaggcg aggaattta gagaggctt 
        tgggtggcg aagaagaag gcgaacctg gagcacctc 
        ctgtaggag cggttggag aaggagaag agcggttta 
        ttgagggtg aagaagcag gagttcctg gaggtcccg 
        gaacagctc caggactcg aaggaacag cttccgaag 
        ctgaaggag ctgaagaag cagttcctg gaaccgaag 
        gaacagctc ctggagctg aaggaatag ttcctggag 
        atgaaggaa tagttcctg gagatgaag gaacagttc 
        ctggtgctg aaggaacaa ttcctggag gtgaagaag 
        caggtcttg ggggtcctg gagcggcag gattcgggg 
        agggtttcg agggagaag aggccgctt tcgggcggg 
        attacggcc aaggtgaag aagatgcat ttggaggtg 
        cagtggcag aaggtgcct cacttggat ttggagaag 
        gtgaaggtg ggtttggcg aagagggag aagttggtt 
        ttggccctg ccgaagtag aagcagcag aagagtatg 
        gtcgtgcag aagaagctt atttttacg gagcgcaag 
        aagatttgt acggcgaag ctgaagatt tgctctatg 
        gtgaagaag gtttcgacg aaggagaag gttatggtc 
        tacctcctg gtgtagaat taggcgaag aaggtgaag 
        aactaatta tgggtcgct tggccgatg agcttgagc 
        gtcttgaca gcctcctgg gtgtaattc ggggacaca 
        aaccatccg tggtttatg aacacactc caggcacca 
        tttgttgtc tctcattga aaatttggg ccgtttggt 
        tgttagaaa tcgccaata atgcccata attggacga 
        aatggattg gggcaataa ttaaacaag tccgccact 
        atgtggcta taatcagag gagatgttt tactcccaa 
        gtggcgaaa agcaagtct tgtacaagg aggagtggc 
        gcaaatttg tcaaagaca ctgaagaaa aagttgtcg 
        cgtggcgac aatacagca aacatgtta aagacttat 
        cgaccgaaa tcatggaaa acttccacg gtaaaaaag 
        ttaattgtt gtccgaaat gtctccaag accatttaa 
        ttaagaacg tggtgacgg cacatgaga ctcttcaag 
        ccctcactg aggctatca actgtgccg ggtattggc 
        agagatgtc tcgatagaa tagttgagc aaacgagta 
        aaatcaccg aaatggaac tgggccctg aaagtgggg 
        cccctgatg taacgtcat tgggttccg aggggtcgt 
        atagtatcg aaattgaag aattggcac acatgccaa 
        cacgtaaag tggcctcag aaggcagtt acggttcca 
        tgtacgaaa ttgaggagt tgccagtac caggaccgg 
        tttattcca actacgaca ttgtcgagg tgttgccaa 
        cgaagcccg aacccagtt attttcacg aaatcaccg 
        aaattgcga ctttggagc ccccaccca agccgcaaa 
        aagtggagg cacccaaac ccgccaaac cacacaaat 
        cgaatttga agggaaaga aaaagaaat aatgtttgt 
        ttacgtctt tattgttat aaattac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY