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Gene information
General information
TC006755 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 588225..597477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006755
    iBeetle screen iB_01087
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006755 RA
      MSKDKTFQE RLKNIFKKK PDLVNVPPN REFILTDEL 
      LRDLGKDAP THTRVKAMK ELAPHLAKL EPNGVEKLW 
      SCIQDMFLR EYPNDVRHA TFGFLQSVI RSQFERIDV 
      MRAQFFRFI KHHDNPQDV AHRVDLLAA LTNDGKEVE 
      HFEEEIGPF LLNWITDVS RVGKLHEFL KILDNVIKF 
      NSPHLDEYT MTGYVKHIC VLCCNTNQF PIVILCLQI 
      LGTVVAYSI LPPESLSQF VGTLCRTVN IKCYCPASW 
      KTMKNLLGT HLGHSTLFT MCRFLQEPN LKHDAGLMR 
      GCVFYIHMA LWDINSMQN LCCPPSSVL PSVLQAVRC 
      NHPSVAYEV ILGIQKLIN KQGLDLLEP TWDIILEII 
      LHIVKQVEL APNEVPNSS TALTLHEIL NTIENLIEV 
      GNYKGSMNQ FFAVIHECS RDRPETSVL KLIKYQSKN 
      IRPTESAWL QNLFNLLSN YFKPEVRTN IRLKVLNVL 
      EDTIRLYGL YEDELINHI VIPHMQNIA QDDNVVVRS 
      EVAKLLVDL CKNYNSNIC TELLEILEK LLQRPPNAN 
      NESDFLDVG VLVPGLVQV FARKIHKLP SSHAVLIYK 
      IFVNFLERN LLKPPFKEF NQSRLMIFD CLLNMRADS 
      YCRLGLPDP DTKQMKYSP YLCVSYKPN EPIVTSPPT 
      TSPSQTPVC KVTFLAIES AFKLLLNYL ENEKDWEIL 
      KLVMEKLPK MLGNKAIVL TKSGNPDLN NLVDVLCTM 
      LQNRSLNLS EVKVTRLEF QTAVVSVMV ALSSYGNSL 
      DQYHQQLLI NTLTRSTGT DPRCSRQSI NSLTICMVD 
      MRETMMKLL PDVLLRLSK ISATKYIAM PVLEFLSTL 
      SQLPNVYAN FVGDQYMAV FAIALPYTN PFKYDNYIV 
      SLAYHVISV WFLKCRLPF RRDFVKFIV SGLQTNVMV 
      PFQELSQFK LSLSSMNQD SSDRKRSSS LTEQGSRRR 
      VKPDPVKKS PFIQKTTPR DKTVLTFYE ELNETCIDL 
      MARYTYSPC SALPSRLPT AEFLLNGGI SKSWMLGNK 
      LITITTSGC TERVLKHGV CDKCWSLCS SHNNSKIAR 
      YARSNSGNE EKESFLTRQ GSSEEGKRY DSANLPKLI 
      GGDDRREAL VCACWCQGW AEVLIRRPT GNVSWVMKV 
      QNQVSFKQH IFEFPLNEM STLYMPDLF FEPSSAISI 
      EEQEETEEF EEAVASSSS RPVSVPGSP TKQSLSRQS 
      SRDSADDEL EFVYDDGTK SRNPVRRSN SSPEMANWK 
      NPFVHQKIE NDSGNPDKK DVRVSCEAI PEEISGLGT 
      SPPTGEPPK QEHPSLLTC YSYPGSSPP GESGIAPKP 
      YKTVPASPN QINSQSQPT FVQETNVLD RPNNLPNLT 
      NLAPLTTKP PQSPTQTSP RLQRHADKE KDGTSIQKS 
      SSSSKIEKN RAKAPDPSL AEKRDRLHT ISVMSTSTR 
      KPTPPKKIP KEILKSGLN PSFVFLQLY HTACFGSTS 
      EVPLYVPSC TVIDRAIGI LDRMYPYEV HKIGVLYIN 
      EGQVEKEAE ILRNTCGCV RYMEFLQNL GTLIRLADV 
      NPRDIYLGG VDQGGADGK YMYTHQDDL VMLAFHVAT 
      LMPNKDSDP NGNYKKAHV GNDNVVIVY NNSGQEFDI 
      STFRSQVTL AYIIVEPLD YQCNKVEVK VKSDLLKII 
      GSNEVIVVS DQSVSILVR QLAIHADLA ATVLNSLRS 
      HATDPYASN WLERLRKLK NLREKVVQE NNKRKEAQK 
      GFSEEKDIV EDFTDFT
    CDS sequence
    • >TC006755 RA
      atgagcaaa gacaagacg tttcaagag cgcctgaag 
      aacatcttc aagaagaag ccagattta gtcaatgtg 
      cccccaaac cgcgagttt atcctaacc gatgagctg 
      ctgcgcgac ctggggaag gacgccccc acccacacg 
      cgcgtcaaa gccatgaag gagctggcc ccgcatttg 
      gccaagctc gagccgaac ggggtggag aagttgtgg 
      agttgcatt caggacatg ttcctgcgg gagtacccc 
      aacgatgtg cggcatgcc acctttggg tttctccag 
      agcgtgatt cggagccag tttgagagg attgatgtg 
      atgagggcg cagtttttt cgctttatt aagcaccat 
      gataacccc caggatgtg gctcatagg gtggatctt 
      ttggccgct ttgaccaat gacgggaag gaagtggag 
      cactttgag gaggaaatt gggccgttt ctgctcaat 
      tggatcacg gatgtgagt cgggtgggg aagttgcac 
      gaatttttg aaaattttg gataatgtg atcaagttt 
      aattcgccg catttggac gagtacaca atgaccggc 
      tacgtcaaa cacatttgc gtcttgtgc tgcaacacc 
      aatcaattc ccgatcgtt attctgtgc ttacaaatt 
      ttgggcacc gttgtggct tacagtatt ttacctcca 
      gagtcatta tcacaattt gtgggtact ttatgtcgc 
      actgtaaac atcaaatgt tactgtccc gcgagttgg 
      aagaccatg aaaaacttg cttgggacc catttgggt 
      cattcgacc ctattcaca atgtgccgt tttttgcaa 
      gaaccgaac ttaaaacac gatgctggg cttatgaga 
      ggctgcgtt ttttacata catatggca ctgtgggac 
      attaattcg atgcaaaat ctttgttgc ccccctagc 
      tctgtccta ccctcagtt ttacaagcg gttcggtgt 
      aaccaccca tcagtggcg tacgaagtg attttaggg 
      atccaaaaa ttgataaat aaacaagga ttggattta 
      ttggagccc acgtgggac ataatcctt gaaataatc 
      ctacacata gtcaaacaa gtcgaattg gccccaaat 
      gaagttcct aacagctca acagcactg acattgcac 
      gaaattttg aacacaatc gagaatcta atcgaagtt 
      ggcaattac aagggttca atgaaccag ttttttgca 
      gtcattcat gaatgttca cgtgatcga cccgaaaca 
      agcgtctta aaactaatc aaataccag tcgaaaaat 
      atcagaccg actgaaagt gcctggtta cagaaccta 
      ttcaattta cttagcaat tatttcaag cccgaagtt 
      cgcactaac atacgcttg aaagtcctg aacgtgctt 
      gaggacacg atacgactt tacggtctc tacgaggac 
      gagctaatc aaccacatt gtcatccca cacatgcaa 
      aatatcgcc caagacgac aatgtcgtg gtccgatct 
      gaagtagcg aagcttttg gttgatttg tgcaaaaac 
      tacaacagt aacatttgc accgaactg ctcgaaatc 
      ctcgaaaag ctactccaa cgcccaccg aacgcaaac 
      aacgaatcg gatttcctg gacgttgga gtcctcgtc 
      ccgggattg gtccaagtc ttcgcgcgc aaaatccac 
      aaattaccg tcaagccac gccgtcttg atttacaaa 
      attttcgtc aacttcctc gaacgcaat ttactcaaa 
      ccgccgttt aaggaattt aatcaaagt cggctcatg 
      attttcgat tgtttgtta aatatgcgg gctgatagt 
      tactgtcgc ctcgggcta cctgatccc gatacgaaa 
      caaatgaag tacagtccg tatttgtgc gtgagttac 
      aagccgaac gaaccgatt gttacgtca ccgccgact 
      acgtcgccc agtcaaacg ccagtttgt aaagtgacg 
      tttctcgca atcgagagt gcctttaaa ttgttgttg 
      aattatttg gaaaatgag aaagattgg gaaattttg 
      aagcttgtt atggaaaaa ttgccgaaa atgttgggc 
      aataaagcg atcgttttg acaaaaagt ggtaatccg 
      gacctgaac aaccttgtc gatgttttg tgtaccatg 
      ttacaaaac cgttcgctg aacttaagc gaagtcaaa 
      gtgactcgt ttggagttt caaaccgcc gtggtgtca 
      gtcatggtg gcactgtcg tcctatgga aacagtctc 
      gaccagtac caccaacag ttgctgata aatacccta 
      actcgaagc accggcacc gacccacgt tgttcccgc 
      cagtcgata aattcccta acaatttgt atggtcgac 
      atgcgtgaa acaatgatg aaactacta cctgacgtt 
      ctcctacgt ctatcaaaa atatccgca actaaatac 
      atcgcaatg ccggtgttg gaatttcta tcaactcta 
      tcgcagtta cctaacgtt tacgcgaat tttgtcggc 
      gaccaatac atggccgtc tttgctatt gcgctcccg 
      tacactaac ccgttcaaa tatgacaat tatatcgtc 
      tcattagcc tatcacgtg atttcggtt tggttccta 
      aagtgtcgt ctgccgttt cggcgcgat tttgtcaag 
      tttatagtg agcggtctg caaacaaac gtcatggtt 
      ccgtttcaa gaattaagt caatttaag ttgagctta 
      agtagcatg aatcaggac agctctgac cggaagcgc 
      agctcgagt ctcaccgaa cagggctcc agacgccgc 
      gttaagccc gaccctgtc aaaaagagc ccctttatc 
      cagaaaaca accccgaga gacaagact gttttaacg 
      ttctacgag gaattgaac gagacttgt attgattta 
      atggcccgg tacacatat tccccgtgt agtgctctg 
      ccctcgcga ctccccacc gctgagttt ttgctaaat 
      gggggcatt tccaagtcg tggatgttg ggcaataaa 
      ttgattacg attacgacg agtggttgc accgaacgt 
      gtgttaaaa cacggcgtt tgtgataag tgttggtcg 
      ttgtgcagt tcgcataat aatagcaaa attgcgaga 
      tatgcaagg agcaatagt gggaatgag gagaaagag 
      tcgtttctg acgcgacaa ggttcgagt gaggagggg 
      aaaaggtac gattcggcc aatctaccg aagctgatt 
      ggcggagat gacaggagg gaggcgcta gtgtgcgcg 
      tgctggtgt caggggtgg gccgaagtg ttgatcagg 
      aggcccacg ggcaacgtg tcctgggtg atgaaggtc 
      cagaaccag gtctcgttc aagcagcac attttcgaa 
      ttccccctc aacgaaatg tccactctc tacatgccc 
      gacctgttc ttcgagcca agctcagcc atctcgatc 
      gaagaacag gaagaaacg gaagaattt gaagaagct 
      gtagcttca tcaagcagt cgtccggtc agcgtacca 
      ggctcaccc accaaacag tctctatct aggcaaagt 
      tcgcgcgat agcgccgac gacgaacta gaattcgtg 
      tacgacgac ggaacaaaa tcacgaaat cccgtccgt 
      cgttcgaat tctagtccc gaaatggca aactggaaa 
      aatcccttc gtgcatcaa aaaatcgaa aacgactcg 
      gggaatccc gacaagaag gacgtcaga gtgagctgt 
      gaggcgatt ccggaggag atttccggt ttagggaca 
      agtccgccg actggagag cccccgaaa caggaacac 
      ccctcactt ctcacgtgt tacagttat ccggggtct 
      tcgccccct ggtgaaagt ggcatagcc cctaaaccc 
      tacaaaacg gtcccggcg tcccccaat caaatcaat 
      tcccagtca cagccgact tttgtacaa gaaacgaac 
      gtccttgac cgacctaat aatttaccc aacttgact 
      aatctagcc cccttgacc accaaaccg ccccagagc 
      cccactcag acctcgccc cgactgcag cggcacgcc 
      gacaaagaa aaagacgga acctcgatt cagaagagc 
      tcgtcttcg tccaagatc gagaaaaat cgtgcgaaa 
      gcgccggac ccgagtttg gccgaaaag cgcgatcgg 
      ttgcacacg atctcggtc atgagcaca tcgacccga 
      aaaccaaca ccaccgaaa aaaatccct aaggaaata 
      ctcaaaagt ggtttaaac cctagtttt gttttcctc 
      caattgtac cacactgcg tgctttggt agcacttcc 
      gaagtgcca ctttacgta ccctcgtgt actgttatc 
      gaccgagcg atcggtatt ttagatcgg atgtatccg 
      tacgaagtg cacaaaatc ggggtcttg tacattaat 
      gaaggccaa gtcgaaaag gaggccgaa attttgcgc 
      aatacgtgt gggtgtgtc cgctatatg gagttcttg 
      cagaacttg ggcactttg attcgattg gccgacgtt 
      aacccacgt gatatttat ctagggggc gtggaccag 
      ggcggggcc gacggcaag tacatgtac acgcatcag 
      gacgatttg gttatgttg gcgtttcat gtggcgact 
      ttgatgccg aataaagat agcgacccg aatggaaat 
      tacaaaaag gcgcacgtt ggcaacgat aacgttgtc 
      attgtgtac aacaacagt gggcaagag tttgacatt 
      agtacgttt aggagtcag gtgactttg gcgtacatc 
      atcgtggag ccactcgat tatcagtgc aataaagtc 
      gaggtcaag gtcaagtca gacttgttg aaaatcatt 
      ggatcaaac gaggttata gttgtttcg gaccaaagt 
      gtgtcaatt ttagtgagg caacttgcc atacatgcc 
      gatttggca gcgactgtt ctcaattcg ctacggagt 
      cacgcgact gatccgtac gcctctaat tggctggaa 
      agactgagg aaattgaaa aatttgaga gagaaggtc 
      gtacaagag aacaataag aggaaagag gcgcagaaa 
      ggcttcagc gaggaaaag gacatcgtg gaggatttt 
      accgacttt acataa
    mRNA sequence
    • >TC006755 RA
      ttacaaaaa ctaggttat atcggaaat tttcaaacc 
      gacgaaaat gtaacaagt tcgcgacaa aatgagcaa 
      agacaagac gtttcaaga gcgcctgaa gaacatctt 
      caagaagaa gccagattt agtcaatgt gcccccaaa 
      ccgcgagtt tatcctaac cgatgagct gctgcgcga 
      cctggggaa ggacgcccc cacccacac gcgcgtcaa 
      agccatgaa ggagctggc cccgcattt ggccaagct 
      cgagccgaa cggggtgga gaagttgtg gagttgcat 
      tcaggacat gttcctgcg ggagtaccc caacgatgt 
      gcggcatgc cacctttgg gtttctcca gagcgtgat 
      tcggagcca gtttgagag gattgatgt gatgagggc 
      gcagttttt tcgctttat taagcacca tgataaccc 
      ccaggatgt ggctcatag ggtggatct tttggccgc 
      tttgaccaa tgacgggaa ggaagtgga gcactttga 
      ggaggaaat tgggccgtt tctgctcaa ttggatcac 
      ggatgtgag tcgggtggg gaagttgca cgaattttt 
      gaaaatttt ggataatgt gatcaagtt taattcgcc 
      gcatttgga cgagtacac aatgaccgg ctacgtcaa 
      acacatttg cgtcttgtg ctgcaacac caatcaatt 
      cccgatcgt tattctgtg cttacaaat tttgggcac 
      cgttgtggc ttacagtat tttacctcc agagtcatt 
      atcacaatt tgtgggtac tttatgtcg cactgtaaa 
      catcaaatg ttactgtcc cgcgagttg gaagaccat 
      gaaaaactt gcttgggac ccatttggg tcattcgac 
      cctattcac aatgtgccg ttttttgca agaaccgaa 
      cttaaaaca cgatgctgg gcttatgag aggctgcgt 
      tttttacat acatatggc actgtggga cattaattc 
      gatgcaaaa tctttgttg cccccctag ctctgtcct 
      accctcagt tttacaagc ggttcggtg taaccaccc 
      atcagtggc gtacgaagt gattttagg gatccaaaa 
      attgataaa taaacaagg attggattt attggagcc 
      cacgtggga cataatcct tgaaataat cctacacat 
      agtcaaaca agtcgaatt ggccccaaa tgaagttcc 
      taacagctc aacagcact gacattgca cgaaatttt 
      gaacacaat cgagaatct aatcgaagt tggcaatta 
      caagggttc aatgaacca gttttttgc agtcattca 
      tgaatgttc acgtgatcg acccgaaac aagcgtctt 
      aaaactaat caaatacca gtcgaaaaa tatcagacc 
      gactgaaag tgcctggtt acagaacct attcaattt 
      acttagcaa ttatttcaa gcccgaagt tcgcactaa 
      catacgctt gaaagtcct gaacgtgct tgaggacac 
      gatacgact ttacggtct ctacgagga cgagctaat 
      caaccacat tgtcatccc acacatgca aaatatcgc 
      ccaagacga caatgtcgt ggtccgatc tgaagtagc 
      gaagctttt ggttgattt gtgcaaaaa ctacaacag 
      taacatttg caccgaact gctcgaaat cctcgaaaa 
      gctactcca acgcccacc gaacgcaaa caacgaatc 
      ggatttcct ggacgttgg agtcctcgt cccgggatt 
      ggtccaagt cttcgcgcg caaaatcca caaattacc 
      gtcaagcca cgccgtctt gatttacaa aattttcgt 
      caacttcct cgaacgcaa tttactcaa accgccgtt 
      taaggaatt taatcaaag tcggctcat gattttcga 
      ttgtttgtt aaatatgcg ggctgatag ttactgtcg 
      cctcgggct acctgatcc cgatacgaa acaaatgaa 
      gtacagtcc gtatttgtg cgtgagtta caagccgaa 
      cgaaccgat tgttacgtc accgccgac tacgtcgcc 
      cagtcaaac gccagtttg taaagtgac gtttctcgc 
      aatcgagag tgcctttaa attgttgtt gaattattt 
      ggaaaatga gaaagattg ggaaatttt gaagcttgt 
      tatggaaaa attgccgaa aatgttggg caataaagc 
      gatcgtttt gacaaaaag tggtaatcc ggacctgaa 
      caaccttgt cgatgtttt gtgtaccat gttacaaaa 
      ccgttcgct gaacttaag cgaagtcaa agtgactcg 
      tttggagtt tcaaaccgc cgtggtgtc agtcatggt 
      ggcactgtc gtcctatgg aaacagtct cgaccagta 
      ccaccaaca gttgctgat aaataccct aactcgaag 
      caccggcac cgacccacg ttgttcccg ccagtcgat 
      aaattccct aacaatttg tatggtcga catgcgtga 
      aacaatgat gaaactact acctgacgt tctcctacg 
      tctatcaaa aatatccgc aactaaata catcgcaat 
      gccggtgtt ggaatttct atcaactct atcgcagtt 
      acctaacgt ttacgcgaa ttttgtcgg cgaccaata 
      catggccgt ctttgctat tgcgctccc gtacactaa 
      cccgttcaa atatgacaa ttatatcgt ctcattagc 
      ctatcacgt gatttcggt ttggttcct aaagtgtcg 
      tctgccgtt tcggcgcga ttttgtcaa gtttatagt 
      gagcggtct gcaaacaaa cgtcatggt tccgtttca 
      agaattaag tcaatttaa gttgagctt aagtagcat 
      gaatcagga cagctctga ccggaagcg cagctcgag 
      tctcaccga acagggctc cagacgccg cgttaagcc 
      cgaccctgt caaaaagag cccctttat ccagaaaac 
      aaccccgag agacaagac tgttttaac gttctacga 
      ggaattgaa cgagacttg tattgattt aatggcccg 
      gtacacata ttccccgtg tagtgctct gccctcgcg 
      actccccac cgctgagtt tttgctaaa tgggggcat 
      ttccaagtc gtggatgtt gggcaataa attgattac 
      gattacgac gagtggttg caccgaacg tgtgttaaa 
      acacggcgt ttgtgataa gtgttggtc gttgtgcag 
      ttcgcataa taatagcaa aattgcgag atatgcaag 
      gagcaatag tgggaatga ggagaaaga gtcgtttct 
      gacgcgaca aggttcgag tgaggaggg gaaaaggta 
      cgattcggc caatctacc gaagctgat tggcggaga 
      tgacaggag ggaggcgct agtgtgcgc gtgctggtg 
      tcaggggtg ggccgaagt gttgatcag gaggcccac 
      gggcaacgt gtcctgggt gatgaaggt ccagaacca 
      ggtctcgtt caagcagca cattttcga attccccct 
      caacgaaat gtccactct ctacatgcc cgacctgtt 
      cttcgagcc aagctcagc catctcgat cgaagaaca 
      ggaagaaac ggaagaatt tgaagaagc tgtagcttc 
      atcaagcag tcgtccggt cagcgtacc aggctcacc 
      caccaaaca gtctctatc taggcaaag ttcgcgcga 
      tagcgccga cgacgaact agaattcgt gtacgacga 
      cggaacaaa atcacgaaa tcccgtccg tcgttcgaa 
      ttctagtcc cgaaatggc aaactggaa aaatccctt 
      cgtgcatca aaaaatcga aaacgactc ggggaatcc 
      cgacaagaa ggacgtcag agtgagctg tgaggcgat 
      tccggagga gatttccgg tttagggac aagtccgcc 
      gactggaga gcccccgaa acaggaaca cccctcact 
      tctcacgtg ttacagtta tccggggtc ttcgccccc 
      tggtgaaag tggcatagc ccctaaacc ctacaaaac 
      ggtcccggc gtcccccaa tcaaatcaa ttcccagtc 
      acagccgac ttttgtaca agaaacgaa cgtccttga 
      ccgacctaa taatttacc caacttgac taatctagc 
      ccccttgac caccaaacc gccccagag ccccactca 
      gacctcgcc ccgactgca gcggcacgc cgacaaaga 
      aaaagacgg aacctcgat tcagaagag ctcgtcttc 
      gtccaagat cgagaaaaa tcgtgcgaa agcgccgga 
      cccgagttt ggccgaaaa gcgcgatcg gttgcacac 
      gatctcggt catgagcac atcgacccg aaaaccaac 
      accaccgaa aaaaatccc taaggaaat actcaaaag 
      tggtttaaa ccctagttt tgttttcct ccaattgta 
      ccacactgc gtgctttgg tagcacttc cgaagtgcc 
      actttacgt accctcgtg tactgttat cgaccgagc 
      gatcggtat tttagatcg gatgtatcc gtacgaagt 
      gcacaaaat cggggtctt gtacattaa tgaaggcca 
      agtcgaaaa ggaggccga aattttgcg caatacgtg 
      tgggtgtgt ccgctatat ggagttctt gcagaactt 
      gggcacttt gattcgatt ggccgacgt taacccacg 
      tgatattta tctaggggg cgtggacca gggcggggc 
      cgacggcaa gtacatgta cacgcatca ggacgattt 
      ggttatgtt ggcgtttca tgtggcgac tttgatgcc 
      gaataaaga tagcgaccc gaatggaaa ttacaaaaa 
      ggcgcacgt tggcaacga taacgttgt cattgtgta 
      caacaacag tgggcaaga gtttgacat tagtacgtt 
      taggagtca ggtgacttt ggcgtacat catcgtgga 
      gccactcga ttatcagtg caataaagt cgaggtcaa 
      ggtcaagtc agacttgtt gaaaatcat tggatcaaa 
      cgaggttat agttgtttc ggaccaaag tgtgtcaat 
      tttagtgag gcaacttgc catacatgc cgatttggc 
      agcgactgt tctcaattc gctacggag tcacgcgac 
      tgatccgta cgcctctaa ttggctgga aagactgag 
      gaaattgaa aaatttgag agagaaggt cgtacaaga 
      gaacaataa gaggaaaga ggcgcagaa aggcttcag 
      cgaggaaaa ggacatcgt ggaggattt taccgactt 
      tacataatt ttttgttgc atttctatt accattacc 
      caatatatt actatcgat tga