| General information | |||
| TC006755 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13775020..13784272 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006755 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01087 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC006755 RA
MSKDKTFQE RLKNIFKKK PDLVNVPPN REFILTDEL LRDLGKDAP THTRVKAMK ELAPHLAKL EPNGVEKLW SCIQDMFLR EYPNDVRHA TFGFLQSVI RSQFERIDV MRAQFFRFI KHHDNPQDV AHRVDLLAA LTNDGKEVE HFEEEIGPF LLNWITDVS RVGKLHEFL KILDNVIKF NSPHLDEYT MTGYVKHIC VLCCNTNQF PIVILCLQI LGTVVAYSI LPPESLSQF VGTLCRTVN IKCYCPASW KTMKNLLGT HLGHSTLFT MCRFLQEPN LKHDAGLMR GCVFYIHMA LWDINSMQN LCCPPSSVL PSVLQAVRC NHPSVAYEV ILGIQKLIN KQGLDLLEP TWDIILEII LHIVKQVEL APNEVPNSS TALTLHEIL NTIENLIEV GNYKGSMNQ FFAVIHECS RDRPETSVL KLIKYQSKN IRPTESAWL QNLFNLLSN YFKPEVRTN IRLKVLNVL EDTIRLYGL YEDELINHI VIPHMQNIA QDDNVVVRS EVAKLLVDL CKNYNSNIC TELLEILEK LLQRPPNAN NESDFLDVG VLVPGLVQV FARKIHKLP SSHAVLIYK IFVNFLERN LLKPPFKEF NQSRLMIFD CLLNMRADS YCRLGLPDP DTKQMKYSP YLCVSYKPN EPIVTSPPT TSPSQTPVC KVTFLAIES AFKLLLNYL ENEKDWEIL KLVMEKLPK MLGNKAIVL TKSGNPDLN NLVDVLCTM LQNRSLNLS EVKVTRLEF QTAVVSVMV ALSSYGNSL DQYHQQLLI NTLTRSTGT DPRCSRQSI NSLTICMVD MRETMMKLL PDVLLRLSK ISATKYIAM PVLEFLSTL SQLPNVYAN FVGDQYMAV FAIALPYTN PFKYDNYIV SLAYHVISV WFLKCRLPF RRDFVKFIV SGLQTNVMV PFQELSQFK LSLSSMNQD SSDRKRSSS LTEQGSRRR VKPDPVKKS PFIQKTTPR DKTVLTFYE ELNETCIDL MARYTYSPC SALPSRLPT AEFLLNGGI SKSWMLGNK LITITTSGC TERVLKHGV CDKCWSLCS SHNNSKIAR YARSNSGNE EKESFLTRQ GSSEEGKRY DSANLPKLI GGDDRREAL VCACWCQGW AEVLIRRPT GNVSWVMKV QNQVSFKQH IFEFPLNEM STLYMPDLF FEPSSAISI EEQEETEEF EEAVASSSS RPVSVPGSP TKQSLSRQS SRDSADDEL EFVYDDGTK SRNPVRRSN SSPEMANWK NPFVHQKIE NDSGNPDKK DVRVSCEAI PEEISGLGT SPPTGEPPK QEHPSLLTC YSYPGSSPP GESGIAPKP YKTVPASPN QINSQSQPT FVQETNVLD RPNNLPNLT NLAPLTTKP PQSPTQTSP RLQRHADKE KDGTSIQKS SSSSKIEKN RAKAPDPSL AEKRDRLHT ISVMSTSTR KPTPPKKIP KEILKSGLN PSFVFLQLY HTACFGSTS EVPLYVPSC TVIDRAIGI LDRMYPYEV HKIGVLYIN EGQVEKEAE ILRNTCGCV RYMEFLQNL GTLIRLADV NPRDIYLGG VDQGGADGK YMYTHQDDL VMLAFHVAT LMPNKDSDP NGNYKKAHV GNDNVVIVY NNSGQEFDI STFRSQVTL AYIIVEPLD YQCNKVEVK VKSDLLKII GSNEVIVVS DQSVSILVR QLAIHADLA ATVLNSLRS HATDPYASN WLERLRKLK NLREKVVQE NNKRKEAQK GFSEEKDIV EDFTDFT
- >TC006755 RA
atgagcaaa gacaagacg tttcaagag cgcctgaag aacatcttc aagaagaag ccagattta gtcaatgtg cccccaaac cgcgagttt atcctaacc gatgagctg ctgcgcgac ctggggaag gacgccccc acccacacg cgcgtcaaa gccatgaag gagctggcc ccgcatttg gccaagctc gagccgaac ggggtggag aagttgtgg agttgcatt caggacatg ttcctgcgg gagtacccc aacgatgtg cggcatgcc acctttggg tttctccag agcgtgatt cggagccag tttgagagg attgatgtg atgagggcg cagtttttt cgctttatt aagcaccat gataacccc caggatgtg gctcatagg gtggatctt ttggccgct ttgaccaat gacgggaag gaagtggag cactttgag gaggaaatt gggccgttt ctgctcaat tggatcacg gatgtgagt cgggtgggg aagttgcac gaatttttg aaaattttg gataatgtg atcaagttt aattcgccg catttggac gagtacaca atgaccggc tacgtcaaa cacatttgc gtcttgtgc tgcaacacc aatcaattc ccgatcgtt attctgtgc ttacaaatt ttgggcacc gttgtggct tacagtatt ttacctcca gagtcatta tcacaattt gtgggtact ttatgtcgc actgtaaac atcaaatgt tactgtccc gcgagttgg aagaccatg aaaaacttg cttgggacc catttgggt cattcgacc ctattcaca atgtgccgt tttttgcaa gaaccgaac ttaaaacac gatgctggg cttatgaga ggctgcgtt ttttacata catatggca ctgtgggac attaattcg atgcaaaat ctttgttgc ccccctagc tctgtccta ccctcagtt ttacaagcg gttcggtgt aaccaccca tcagtggcg tacgaagtg attttaggg atccaaaaa ttgataaat aaacaagga ttggattta ttggagccc acgtgggac ataatcctt gaaataatc ctacacata gtcaaacaa gtcgaattg gccccaaat gaagttcct aacagctca acagcactg acattgcac gaaattttg aacacaatc gagaatcta atcgaagtt ggcaattac aagggttca atgaaccag ttttttgca gtcattcat gaatgttca cgtgatcga cccgaaaca agcgtctta aaactaatc aaataccag tcgaaaaat atcagaccg actgaaagt gcctggtta cagaaccta ttcaattta cttagcaat tatttcaag cccgaagtt cgcactaac atacgcttg aaagtcctg aacgtgctt gaggacacg atacgactt tacggtctc tacgaggac gagctaatc aaccacatt gtcatccca cacatgcaa aatatcgcc caagacgac aatgtcgtg gtccgatct gaagtagcg aagcttttg gttgatttg tgcaaaaac tacaacagt aacatttgc accgaactg ctcgaaatc ctcgaaaag ctactccaa cgcccaccg aacgcaaac aacgaatcg gatttcctg gacgttgga gtcctcgtc ccgggattg gtccaagtc ttcgcgcgc aaaatccac aaattaccg tcaagccac gccgtcttg atttacaaa attttcgtc aacttcctc gaacgcaat ttactcaaa ccgccgttt aaggaattt aatcaaagt cggctcatg attttcgat tgtttgtta aatatgcgg gctgatagt tactgtcgc ctcgggcta cctgatccc gatacgaaa caaatgaag tacagtccg tatttgtgc gtgagttac aagccgaac gaaccgatt gttacgtca ccgccgact acgtcgccc agtcaaacg ccagtttgt aaagtgacg tttctcgca atcgagagt gcctttaaa ttgttgttg aattatttg gaaaatgag aaagattgg gaaattttg aagcttgtt atggaaaaa ttgccgaaa atgttgggc aataaagcg atcgttttg acaaaaagt ggtaatccg gacctgaac aaccttgtc gatgttttg tgtaccatg ttacaaaac cgttcgctg aacttaagc gaagtcaaa gtgactcgt ttggagttt caaaccgcc gtggtgtca gtcatggtg gcactgtcg tcctatgga aacagtctc gaccagtac caccaacag ttgctgata aatacccta actcgaagc accggcacc gacccacgt tgttcccgc cagtcgata aattcccta acaatttgt atggtcgac atgcgtgaa acaatgatg aaactacta cctgacgtt ctcctacgt ctatcaaaa atatccgca actaaatac atcgcaatg ccggtgttg gaatttcta tcaactcta tcgcagtta cctaacgtt tacgcgaat tttgtcggc gaccaatac atggccgtc tttgctatt gcgctcccg tacactaac ccgttcaaa tatgacaat tatatcgtc tcattagcc tatcacgtg atttcggtt tggttccta aagtgtcgt ctgccgttt cggcgcgat tttgtcaag tttatagtg agcggtctg caaacaaac gtcatggtt ccgtttcaa gaattaagt caatttaag ttgagctta agtagcatg aatcaggac agctctgac cggaagcgc agctcgagt ctcaccgaa cagggctcc agacgccgc gttaagccc gaccctgtc aaaaagagc ccctttatc cagaaaaca accccgaga gacaagact gttttaacg ttctacgag gaattgaac gagacttgt attgattta atggcccgg tacacatat tccccgtgt agtgctctg ccctcgcga ctccccacc gctgagttt ttgctaaat gggggcatt tccaagtcg tggatgttg ggcaataaa ttgattacg attacgacg agtggttgc accgaacgt gtgttaaaa cacggcgtt tgtgataag tgttggtcg ttgtgcagt tcgcataat aatagcaaa attgcgaga tatgcaagg agcaatagt gggaatgag gagaaagag tcgtttctg acgcgacaa ggttcgagt gaggagggg aaaaggtac gattcggcc aatctaccg aagctgatt ggcggagat gacaggagg gaggcgcta gtgtgcgcg tgctggtgt caggggtgg gccgaagtg ttgatcagg aggcccacg ggcaacgtg tcctgggtg atgaaggtc cagaaccag gtctcgttc aagcagcac attttcgaa ttccccctc aacgaaatg tccactctc tacatgccc gacctgttc ttcgagcca agctcagcc atctcgatc gaagaacag gaagaaacg gaagaattt gaagaagct gtagcttca tcaagcagt cgtccggtc agcgtacca ggctcaccc accaaacag tctctatct aggcaaagt tcgcgcgat agcgccgac gacgaacta gaattcgtg tacgacgac ggaacaaaa tcacgaaat cccgtccgt cgttcgaat tctagtccc gaaatggca aactggaaa aatcccttc gtgcatcaa aaaatcgaa aacgactcg gggaatccc gacaagaag gacgtcaga gtgagctgt gaggcgatt ccggaggag atttccggt ttagggaca agtccgccg actggagag cccccgaaa caggaacac ccctcactt ctcacgtgt tacagttat ccggggtct tcgccccct ggtgaaagt ggcatagcc cctaaaccc tacaaaacg gtcccggcg tcccccaat caaatcaat tcccagtca cagccgact tttgtacaa gaaacgaac gtccttgac cgacctaat aatttaccc aacttgact aatctagcc cccttgacc accaaaccg ccccagagc cccactcag acctcgccc cgactgcag cggcacgcc gacaaagaa aaagacgga acctcgatt cagaagagc tcgtcttcg tccaagatc gagaaaaat cgtgcgaaa gcgccggac ccgagtttg gccgaaaag cgcgatcgg ttgcacacg atctcggtc atgagcaca tcgacccga aaaccaaca ccaccgaaa aaaatccct aaggaaata ctcaaaagt ggtttaaac cctagtttt gttttcctc caattgtac cacactgcg tgctttggt agcacttcc gaagtgcca ctttacgta ccctcgtgt actgttatc gaccgagcg atcggtatt ttagatcgg atgtatccg tacgaagtg cacaaaatc ggggtcttg tacattaat gaaggccaa gtcgaaaag gaggccgaa attttgcgc aatacgtgt gggtgtgtc cgctatatg gagttcttg cagaacttg ggcactttg attcgattg gccgacgtt aacccacgt gatatttat ctagggggc gtggaccag ggcggggcc gacggcaag tacatgtac acgcatcag gacgatttg gttatgttg gcgtttcat gtggcgact ttgatgccg aataaagat agcgacccg aatggaaat tacaaaaag gcgcacgtt ggcaacgat aacgttgtc attgtgtac aacaacagt gggcaagag tttgacatt agtacgttt aggagtcag gtgactttg gcgtacatc atcgtggag ccactcgat tatcagtgc aataaagtc gaggtcaag gtcaagtca gacttgttg aaaatcatt ggatcaaac gaggttata gttgtttcg gaccaaagt gtgtcaatt ttagtgagg caacttgcc atacatgcc gatttggca gcgactgtt ctcaattcg ctacggagt cacgcgact gatccgtac gcctctaat tggctggaa agactgagg aaattgaaa aatttgaga gagaaggtc gtacaagag aacaataag aggaaagag gcgcagaaa ggcttcagc gaggaaaag gacatcgtg gaggatttt accgacttt acataa
- >TC006755 RA
ttacaaaaa ctaggttat atcggaaat tttcaaacc gacgaaaat gtaacaagt tcgcgacaa aatgagcaa agacaagac gtttcaaga gcgcctgaa gaacatctt caagaagaa gccagattt agtcaatgt gcccccaaa ccgcgagtt tatcctaac cgatgagct gctgcgcga cctggggaa ggacgcccc cacccacac gcgcgtcaa agccatgaa ggagctggc cccgcattt ggccaagct cgagccgaa cggggtgga gaagttgtg gagttgcat tcaggacat gttcctgcg ggagtaccc caacgatgt gcggcatgc cacctttgg gtttctcca gagcgtgat tcggagcca gtttgagag gattgatgt gatgagggc gcagttttt tcgctttat taagcacca tgataaccc ccaggatgt ggctcatag ggtggatct tttggccgc tttgaccaa tgacgggaa ggaagtgga gcactttga ggaggaaat tgggccgtt tctgctcaa ttggatcac ggatgtgag tcgggtggg gaagttgca cgaattttt gaaaatttt ggataatgt gatcaagtt taattcgcc gcatttgga cgagtacac aatgaccgg ctacgtcaa acacatttg cgtcttgtg ctgcaacac caatcaatt cccgatcgt tattctgtg cttacaaat tttgggcac cgttgtggc ttacagtat tttacctcc agagtcatt atcacaatt tgtgggtac tttatgtcg cactgtaaa catcaaatg ttactgtcc cgcgagttg gaagaccat gaaaaactt gcttgggac ccatttggg tcattcgac cctattcac aatgtgccg ttttttgca agaaccgaa cttaaaaca cgatgctgg gcttatgag aggctgcgt tttttacat acatatggc actgtggga cattaattc gatgcaaaa tctttgttg cccccctag ctctgtcct accctcagt tttacaagc ggttcggtg taaccaccc atcagtggc gtacgaagt gattttagg gatccaaaa attgataaa taaacaagg attggattt attggagcc cacgtggga cataatcct tgaaataat cctacacat agtcaaaca agtcgaatt ggccccaaa tgaagttcc taacagctc aacagcact gacattgca cgaaatttt gaacacaat cgagaatct aatcgaagt tggcaatta caagggttc aatgaacca gttttttgc agtcattca tgaatgttc acgtgatcg acccgaaac aagcgtctt aaaactaat caaatacca gtcgaaaaa tatcagacc gactgaaag tgcctggtt acagaacct attcaattt acttagcaa ttatttcaa gcccgaagt tcgcactaa catacgctt gaaagtcct gaacgtgct tgaggacac gatacgact ttacggtct ctacgagga cgagctaat caaccacat tgtcatccc acacatgca aaatatcgc ccaagacga caatgtcgt ggtccgatc tgaagtagc gaagctttt ggttgattt gtgcaaaaa ctacaacag taacatttg caccgaact gctcgaaat cctcgaaaa gctactcca acgcccacc gaacgcaaa caacgaatc ggatttcct ggacgttgg agtcctcgt cccgggatt ggtccaagt cttcgcgcg caaaatcca caaattacc gtcaagcca cgccgtctt gatttacaa aattttcgt caacttcct cgaacgcaa tttactcaa accgccgtt taaggaatt taatcaaag tcggctcat gattttcga ttgtttgtt aaatatgcg ggctgatag ttactgtcg cctcgggct acctgatcc cgatacgaa acaaatgaa gtacagtcc gtatttgtg cgtgagtta caagccgaa cgaaccgat tgttacgtc accgccgac tacgtcgcc cagtcaaac gccagtttg taaagtgac gtttctcgc aatcgagag tgcctttaa attgttgtt gaattattt ggaaaatga gaaagattg ggaaatttt gaagcttgt tatggaaaa attgccgaa aatgttggg caataaagc gatcgtttt gacaaaaag tggtaatcc ggacctgaa caaccttgt cgatgtttt gtgtaccat gttacaaaa ccgttcgct gaacttaag cgaagtcaa agtgactcg tttggagtt tcaaaccgc cgtggtgtc agtcatggt ggcactgtc gtcctatgg aaacagtct cgaccagta ccaccaaca gttgctgat aaataccct aactcgaag caccggcac cgacccacg ttgttcccg ccagtcgat aaattccct aacaatttg tatggtcga catgcgtga aacaatgat gaaactact acctgacgt tctcctacg tctatcaaa aatatccgc aactaaata catcgcaat gccggtgtt ggaatttct atcaactct atcgcagtt acctaacgt ttacgcgaa ttttgtcgg cgaccaata catggccgt ctttgctat tgcgctccc gtacactaa cccgttcaa atatgacaa ttatatcgt ctcattagc ctatcacgt gatttcggt ttggttcct aaagtgtcg tctgccgtt tcggcgcga ttttgtcaa gtttatagt gagcggtct gcaaacaaa cgtcatggt tccgtttca agaattaag tcaatttaa gttgagctt aagtagcat gaatcagga cagctctga ccggaagcg cagctcgag tctcaccga acagggctc cagacgccg cgttaagcc cgaccctgt caaaaagag cccctttat ccagaaaac aaccccgag agacaagac tgttttaac gttctacga ggaattgaa cgagacttg tattgattt aatggcccg gtacacata ttccccgtg tagtgctct gccctcgcg actccccac cgctgagtt tttgctaaa tgggggcat ttccaagtc gtggatgtt gggcaataa attgattac gattacgac gagtggttg caccgaacg tgtgttaaa acacggcgt ttgtgataa gtgttggtc gttgtgcag ttcgcataa taatagcaa aattgcgag atatgcaag gagcaatag tgggaatga ggagaaaga gtcgtttct gacgcgaca aggttcgag tgaggaggg gaaaaggta cgattcggc caatctacc gaagctgat tggcggaga tgacaggag ggaggcgct agtgtgcgc gtgctggtg tcaggggtg ggccgaagt gttgatcag gaggcccac gggcaacgt gtcctgggt gatgaaggt ccagaacca ggtctcgtt caagcagca cattttcga attccccct caacgaaat gtccactct ctacatgcc cgacctgtt cttcgagcc aagctcagc catctcgat cgaagaaca ggaagaaac ggaagaatt tgaagaagc tgtagcttc atcaagcag tcgtccggt cagcgtacc aggctcacc caccaaaca gtctctatc taggcaaag ttcgcgcga tagcgccga cgacgaact agaattcgt gtacgacga cggaacaaa atcacgaaa tcccgtccg tcgttcgaa ttctagtcc cgaaatggc aaactggaa aaatccctt cgtgcatca aaaaatcga aaacgactc ggggaatcc cgacaagaa ggacgtcag agtgagctg tgaggcgat tccggagga gatttccgg tttagggac aagtccgcc gactggaga gcccccgaa acaggaaca cccctcact tctcacgtg ttacagtta tccggggtc ttcgccccc tggtgaaag tggcatagc ccctaaacc ctacaaaac ggtcccggc gtcccccaa tcaaatcaa ttcccagtc acagccgac ttttgtaca agaaacgaa cgtccttga ccgacctaa taatttacc caacttgac taatctagc ccccttgac caccaaacc gccccagag ccccactca gacctcgcc ccgactgca gcggcacgc cgacaaaga aaaagacgg aacctcgat tcagaagag ctcgtcttc gtccaagat cgagaaaaa tcgtgcgaa agcgccgga cccgagttt ggccgaaaa gcgcgatcg gttgcacac gatctcggt catgagcac atcgacccg aaaaccaac accaccgaa aaaaatccc taaggaaat actcaaaag tggtttaaa ccctagttt tgttttcct ccaattgta ccacactgc gtgctttgg tagcacttc cgaagtgcc actttacgt accctcgtg tactgttat cgaccgagc gatcggtat tttagatcg gatgtatcc gtacgaagt gcacaaaat cggggtctt gtacattaa tgaaggcca agtcgaaaa ggaggccga aattttgcg caatacgtg tgggtgtgt ccgctatat ggagttctt gcagaactt gggcacttt gattcgatt ggccgacgt taacccacg tgatattta tctaggggg cgtggacca gggcggggc cgacggcaa gtacatgta cacgcatca ggacgattt ggttatgtt ggcgtttca tgtggcgac tttgatgcc gaataaaga tagcgaccc gaatggaaa ttacaaaaa ggcgcacgt tggcaacga taacgttgt cattgtgta caacaacag tgggcaaga gtttgacat tagtacgtt taggagtca ggtgacttt ggcgtacat catcgtgga gccactcga ttatcagtg caataaagt cgaggtcaa ggtcaagtc agacttgtt gaaaatcat tggatcaaa cgaggttat agttgtttc ggaccaaag tgtgtcaat tttagtgag gcaacttgc catacatgc cgatttggc agcgactgt tctcaattc gctacggag tcacgcgac tgatccgta cgcctctaa ttggctgga aagactgag gaaattgaa aaatttgag agagaaggt cgtacaaga gaacaataa gaggaaaga ggcgcagaa aggcttcag cgaggaaaa ggacatcgt ggaggattt taccgactt tacataatt ttttgttgc atttctatt accattacc caatatatt actatcgat tga
