Status
Please wait ...
Gene information
General information
TC006736 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 381613..383375
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006736
    iBeetle screen iB_01085
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    elytra slightly shortened - Number of animals showing the phenotype: 3


    elytra shape irregular - Number of animals showing the phenotype: 3

    elytra surface irregular - Number of animals showing the phenotype: 10


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006736 RA
      MANLRQTPL TCSGHTRPV VHLDFSSVT KYGYFLISA 
      CKDGKPMLR QGDTGDWIG TFEGHKGAV WGVALNKDA 
      TKAASGAAD FTGKVWDAN SGEELHSFQ HKHIVKSVN 
      FSKDSALLA TGSNEKIVR IFDLNKPEA KPEEFTGHT 
      SGIRHVIFF RNDTHLVSC ADDKTLRVW DRSSGKEVQ 
      KMDFPSIPN SLEVSRDGT VLTVTHSNH VTFLDSESL 
      NKIREFSVP TTVNSASLH PDKTIFVAG GDDLKVYKF 
      DYTTGNEIE SFKGHFGPV HCVKFSPDG ELYASGSED 
      GTLRLWQTT IGKTYGLWK CVDGPQINN DAITSPKEV 
      PAI
    CDS sequence
    • >TC006736 RA
      atggcaaat cttcgtcaa accccctta acctgtagc 
      ggccacact cgtcccgtc gtccatctc gacttcagt 
      tcggtgaca aaatacggc tatttccta atttcggca 
      tgcaaagac ggcaaaccc atgctaagg caaggggac 
      accggcgac tggatcggc accttcgag ggccacaag 
      ggggcggtt tggggcgtc gcgctcaac aaggacgcc 
      accaaggcc gccagcggc gccgccgac ttcaccggc 
      aaggtctgg gacgccaac tccggcgag gaactgcac 
      tccttccag cataaacac atcgtcaaa agcgtcaac 
      ttttccaaa gacagtgcc ttattggcg acggggagc 
      aacgaaaaa attgtccga attttcgat ctgaataaa 
      cccgaagcg aaacccgag gagtttacg gggcacact 
      tccgggata agacacgtg atctttttc aggaatgac 
      acgcatctt gtctcgtgt gcggacgat aagactttg 
      agggtttgg gaccggagt tccggaaag gaagtccag 
      aaaatggac ttcccgtcg attccgaac agccttgag 
      gtgtccaga gacggcacc gtgctcaca gtaacccac 
      tcaaatcac gtcacattc ctcgactcc gaatctcta 
      aacaaaatt agagaattt tccgttcct acgacagtc 
      aattcggct tcgctacac cccgacaaa acgattttc 
      gtcgccggt ggcgatgat ttgaaagtc tacaaattc 
      gattacaca actggaaat gaaatcgaa tcgttcaag 
      ggccacttt ggccccgtc cattgtgtc aaattctcg 
      cccgacggt gaattgtac gcttccggg tcagaagac 
      ggcacttta agactttgg caaaccaca attggcaag 
      acttatgga ctttggaaa tgcgttgac gggccccaa 
      atcaacaac gacgcgatt acgtcaccc aaagaagta 
      cccgcgatt tag
    mRNA sequence
    • >TC006736 RA
      tttcccagc tgttgttga cgctgtcaa attcagttc 
      gctttctga actcctcgt gttagtgtg gtgtgtagg 
      tttttagta taatttttt tgtaccgtt tgacctaat 
      aaaatagaa aactatggc aaatcttcg tcaaacccc 
      cttaacctg tagcggcca cactcgtcc cgtcgtcca 
      tctcgactt cagttcggt gacaaaata cggctattt 
      cctaatttc ggcatgcaa agacggcaa acccatgct 
      aaggcaagg ggacaccgg cgactggat cggcacctt 
      cgagggcca caagggggc ggtttgggg cgtcgcgct 
      caacaagga cgccaccaa ggccgccag cggcgccgc 
      cgacttcac cggcaaggt ctgggacgc caactccgg 
      cgaggaact gcactcctt ccagcataa acacatcgt 
      caaaagcgt caacttttc caaagacag tgccttatt 
      ggcgacggg gagcaacga aaaaattgt ccgaatttt 
      cgatctgaa taaacccga agcgaaacc cgaggagtt 
      tacggggca cacttccgg gataagaca cgtgatctt 
      tttcaggaa tgacacgca tcttgtctc gtgtgcgga 
      cgataagac tttgagggt ttgggaccg gagttccgg 
      aaaggaagt ccagaaaat ggacttccc gtcgattcc 
      gaacagcct tgaggtgtc cagagacgg caccgtgct 
      cacagtaac ccactcaaa tcacgtcac attcctcga 
      ctccgaatc tctaaacaa aattagaga attttccgt 
      tcctacgac agtcaattc ggcttcgct acaccccga 
      caaaacgat tttcgtcgc cggtggcga tgatttgaa 
      agtctacaa attcgatta cacaactgg aaatgaaat 
      cgaatcgtt caagggcca ctttggccc cgtccattg 
      tgtcaaatt ctcgcccga cggtgaatt gtacgcttc 
      cgggtcaga agacggcac tttaagact ttggcaaac 
      cacaattgg caagactta tggactttg gaaatgcgt 
      tgacgggcc ccaaatcaa caacgacgc gattacgtc 
      acccaaaga agtacccgc gatttagct gtttgacga 
      atttgtttt ctcctcctt cctcaaatc caagtaact 
      cgtagttct ttcacgatt aggtgaaca gaagacgaa 
      aaagcgacg gaaaactga tttatttat gttactatt 
      tgtttgaat atttaataa ataatcacg aaaatt