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Gene information
General information
TC006730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 263189..265271
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006730
    iBeetle screen iB_01084
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006730 RA
      MSEGDVTTP DEFEHENET NYKPPPEKT 
      IEEILQADQ DDESLRKYK ETLLGQAQT 
      GPVIVEPDN PRKVIVKRL VLVVADRPE 
      IALDLTGDL SRLKKETFV IKEGVSYRI 
      RIEFIVQRE IVHGLKYVQ KTSKLGMSV 
      DKMTHMVGS YAPKMEIQS YTTPPEDAP 
      TGMLARGSY TVHSLFTDD DKNEHLKWE 
      WSFEIKKDW KD
    • >TC006730 RB
      MSEGDVTTP DEFEHENET NYKPPPEKT IEEILQADQ 
      DDESLRKYK ETLLGQAQT GPVIVEPDN PRKVIVKRL 
      VLVVADRPE IALDLTGDL SRLKKETFV IKEGVSYRI 
      RIEFIVQRE IVHGLKYVQ KTSKLGMSG ECVCEIART 
      LACLLGSGL IGGKMVQQL NTYSILVGI DEKATTKML 
      YFTWCVFIV DKMTHMVGS YAPKMEIQS YTTPPEDAP 
      TGMLARGSY TVHSLFTDD DKNEHLKWE WSFEIKKDW 
      KD
    CDS sequence
    • >TC006730 RA
      atgtccgaa ggcgacgtc acgaccccc gacgagttc 
      gagcacgaa aacgagaca aattacaag cccccgccc 
      gaaaaaacc atcgaggag atcctgcag gcggaccag 
      gacgatgaa agtctgcgg aagtacaag gagacgctc 
      ctggggcag gcccagacc gggcctgtg atcgttgag 
      cctgataac ccccgcaaa gtcatcgtg aagcggctg 
      gttttggtg gtggccgat aggccggaa atcgcgctg 
      gatttgacg ggggacttg tcccggttg aagaaagag 
      actttcgtg attaaggag ggcgtttcg tacagaatc 
      cggatagag tttatcgtc cagagggag atcgtgcat 
      gggcttaag tacgtgcaa aagaccagc aaactcgga 
      atgtcagtg gacaaaatg acccatatg gtcggctca 
      tacgccccc aaaatggaa attcagtcc tacaccaca 
      ccaccagag gatgccccc acggggatg ttagcacgt 
      ggttcgtac acagtccac tccctgttc accgacgat 
      gacaaaaac gagcatctc aaatgggag tggagcttc 
      gaaatcaaa aaagattgg aaagattaa
    • >TC006730 RB
      atgtccgaa ggcgacgtc acgaccccc gacgagttc 
      gagcacgaa aacgagaca aattacaag cccccgccc 
      gaaaaaacc atcgaggag atcctgcag gcggaccag 
      gacgatgaa agtctgcgg aagtacaag gagacgctc 
      ctggggcag gcccagacc gggcctgtg atcgttgag 
      cctgataac ccccgcaaa gtcatcgtg aagcggctg 
      gttttggtg gtggccgat aggccggaa atcgcgctg 
      gatttgacg ggggacttg tcccggttg aagaaagag 
      actttcgtg attaaggag ggcgtttcg tacagaatc 
      cggatagag tttatcgtc cagagggag atcgtgcat 
      gggcttaag tacgtgcaa aagaccagc aaactcgga 
      atgtcaggt gagtgcgtt tgcgaaatt gctcggaca 
      ctcgcgtgc ctgcttgga agtggactt attgggggt 
      aaaatggta caacagctc aacacttat tctattctt 
      gtggggatt gacgaaaag gccaccaca aaaatgctt 
      tattttacg tggtgtgtg tttatagtg gacaaaatg 
      acccatatg gtcggctca tacgccccc aaaatggaa 
      attcagtcc tacaccaca ccaccagag gatgccccc 
      acggggatg ttagcacgt ggttcgtac acagtccac 
      tccctgttc accgacgat gacaaaaac gagcatctc 
      aaatgggag tggagcttc gaaatcaaa aaagattgg 
      aaagattaa
    mRNA sequence
    • >TC006730 RA
      cgcaatcgc cgaaaacta acagtagga ggttgacac 
      ccacttttt gcacacaaa aaccgcaat aacaccttt 
      aaacaaaca cacagcata aaattggtg ccccccttc 
      agccgaata accccccgg cgtgtgtta tcactaaaa 
      tcgcagcca caatgtccg aaggcgacg tcacgaccc 
      ccgacgagt tcgagcacg aaaacgaga caaattaca 
      agcccccgc ccgaaaaaa ccatcgagg agatcctgc 
      aggcggacc aggacgatg aaagtctgc ggaagtaca 
      aggagacgc tcctggggc aggcccaga ccgggcctg 
      tgatcgttg agcctgata acccccgca aagtcatcg 
      tgaagcggc tggttttgg tggtggccg ataggccgg 
      aaatcgcgc tggatttga cgggggact tgtcccggt 
      tgaagaaag agactttcg tgattaagg agggcgttt 
      cgtacagaa tccggatag agtttatcg tccagaggg 
      agatcgtgc atgggctta agtacgtgc aaaagacca 
      gcaaactcg gaatgtcag tggacaaaa tgacccata 
      tggtcggct catacgccc ccaaaatgg aaattcagt 
      cctacacca caccaccag aggatgccc ccacgggga 
      tgttagcac gtggttcgt acacagtcc actccctgt 
      tcaccgacg atgacaaaa acgagcatc tcaaatggg 
      agtggagct tcgaaatca aaaaagatt ggaaagatt 
      aaagccaaa acaagtatt actattaac cgcaataaa 
      tttatatac ctacctttt ataataagt atacagacc 
      ttttgacaa ttttacaag acgtcagta tgccttaac 
      gtacaattt tctatcctt tgctcattc aaaaatgcc 
      ttacctgtg ttagtttgc tgatgtata ttgcattta 
      tcgtactga tcaagcttt agaccaatt aagtgtgtt 
      gctactttc aaataaaac aaaatgaaa agg
    • >TC006730 RB
      cgcaatcgc cgaaaacta acagtagga ggttgacac 
      ccacttttt gcacacaaa aaccgcaat aacaccttt 
      aaacaaaca cacagcata aaattggtg ccccccttc 
      agccgaata accccccgg cgtgtgtta tcactaaaa 
      tcgcagcca caatgtccg aaggcgacg tcacgaccc 
      ccgacgagt tcgagcacg aaaacgaga caaattaca 
      agcccccgc ccgaaaaaa ccatcgagg agatcctgc 
      aggcggacc aggacgatg aaagtctgc ggaagtaca 
      aggagacgc tcctggggc aggcccaga ccgggcctg 
      tgatcgttg agcctgata acccccgca aagtcatcg 
      tgaagcggc tggttttgg tggtggccg ataggccgg 
      aaatcgcgc tggatttga cgggggact tgtcccggt 
      tgaagaaag agactttcg tgattaagg agggcgttt 
      cgtacagaa tccggatag agtttatcg tccagaggg 
      agatcgtgc atgggctta agtacgtgc aaaagacca 
      gcaaactcg gaatgtcag gtgagtgcg tttgcgaaa 
      ttgctcgga cactcgcgt gcctgcttg gaagtggac 
      ttattgggg gtaaaatgg tacaacagc tcaacactt 
      attctattc ttgtgggga ttgacgaaa aggccacca 
      caaaaatgc tttatttta cgtggtgtg tgtttatag 
      tggacaaaa tgacccata tggtcggct catacgccc 
      ccaaaatgg aaattcagt cctacacca caccaccag 
      aggatgccc ccacgggga tgttagcac gtggttcgt 
      acacagtcc actccctgt tcaccgacg atgacaaaa 
      acgagcatc tcaaatggg agtggagct tcgaaatca 
      aaaaagatt ggaaagatt aaagccaaa acaagtatt 
      actattaac cgcaataaa tttatatac ctacctttt 
      ataataagt atacagacc ttttgacaa ttttacaag 
      acgtcagta tgccttaac gtacaattt tctatcctt 
      tgctcattc aaaaatgcc ttacctgtg ttagtttgc 
      tgatgtata ttgcattta tcgtactga tcaagcttt 
      agaccaatt aagtgtgtt gctactttc aaataaaac 
      aaaatgaaa agg