Status
Please wait ...
Gene information
General information
TC006714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13230401..13237730
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006714
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006714 RA
      MFFDQSGLA VAAPPPDVP ELMGIESEY VQTQCILAD 
      GGDRFGVSA LAFDRYEEL LWMGNQGGH VTSYYTGAM 
      QKYTSFQIH ATEDVRQVL TINEGVLGL TSSSLRYQI 
      RRGIPVFTH TSANMYEMQ CMLQVGATR LLMGGHHGK 
      LIDFNIDLC KEMQVVDVG EPGCVMLRP HSRFVCAGN 
      PLGRIDLRD PNTLNTEHT LETHSGSLS DFDVQGNLL 
      VTCGFSNRH GNLTVDRFL MVYDLRMMR AVTPIQTVL 
      DPLYLRFLP SVSSRLAVV SALGHAQIL DTVAFAEPR 
      LCLMQMDSP AMCLTFDIS ATSQALAFG DSTGSIHLF 
      SSTAQPLFN SYSRMTEHG DPLVTYPSF AIDDFETPL 
      AVIPMPICH DGLSSDWPE HLMKKCYRR PVGVDPEVL 
      RTMKMKGPI GYAPNPRTT RRNQVMYEI DNNKARALN 
      KHDRNAKLD DSEGFIAIP KRYRKIDVK YNKLGTDEC 
      QFDQFNKTG HTGLEATLP NAYCNAMIQ VLYYIEPIR 
      AVLLSHLCN KEFCLSCEL GFLFHMLRT SSNNSPCQP 
      ANFLRAFRT IPEASALSL VYSDLPPDQ KTKINLGAL 
      IQSWNRFII HQLHVELAE AKKKNTERK NNKTPFSYN 
      VTDFPAIDD RKKCSASEV NANMEQEKR DSESEIIQL 
      LGAKQIQKH SCLKCKLEV KKESILLVC NLIYPTNEP 
      ERDEWSFCD ILTRSLCSQ QTTPAWCEE CKKFAPTQQ 
      TRVLQSLPK LLAINTGLH NPQHKQFWQ SQMDKVVAK 
      VTENAAPPT APQINSLSS SKPCRYGDH CSRPGCRFR 
      HSFDNVPPP APVNNPYCS NNWLPHSIS IALKEGELI 
      IEKVEDKKE VEGEERVED ELYDLTAVV CYINDPASS 
      EKRNIVALI KVPDSYLSE TTQSEHKWY LFNDFSIST 
      VSMQEAVWF SLDWKIPCI LYYSSRGIL KAKSEISPP 
      ISKEVFTED ACIARSWGT SAITFTPLT DNEFPKKGD 
      LVAMDAEFV TLNQEEAEL RSDGKMSMI KPSQMSVAR 
      ITCIRGSGP MEGVPFIDD YISTQEQVV DYLTKFSGI 
      KPGDLDANF SSKHLTTLK STYTKLRFL LDSGVIFVG 
      HGLKNDFRV INLVVPPEQ VADTVHLFH LPHHRMVSL 
      RFLAWHFLG VKIQSETHD SVEDARAAL HLYKKYKQL 
      DAQSKVGEA LAELYERGK LLNWKVPED
    CDS sequence
    • >TC006714 RA
      atgtttttc gaccaaagc gggcttgcg gtggcggcg 
      ccgcccccg gacgtgccc gaactgatg gggatcgag 
      tcagaatac gtccaaacg cagtgtatt ctagcggat 
      gggggcgac cgattcggg gtgagcgcc ctggcgttt 
      gaccgctat gaggagctc ctctggatg ggcaaccag 
      ggcggccac gtcacgtcc tactacacc ggcgccatg 
      cagaaatac acctcgttc caaatccac gcgactgaa 
      gacgtgcga caggtactt acaataaac gagggggtt 
      ttagggcta acttccagt agtctaagg tatcagata 
      aggcgcggg atccccgtc ttcacccac acttctgcc 
      aacatgtac gaaatgcaa tgtatgcta caagtgggg 
      gcgacgagg ctcctgatg ggggggcac cacggcaaa 
      ctgatcgat tttaacatc gatttgtgc aaggaaatg 
      caagttgtt gatgtgggg gagcctggc tgcgttatg 
      ttgcgcccc catagtagg tttgtttgc gcggggaac 
      cccttgggc aggattgac ctgagggac cccaacaca 
      ctcaacact gaacatacg ctagagacg cacagtggc 
      agtctgagc gattttgac gtccagggg aaccttctg 
      gtgacttgt ggcttcagc aataggcac ggcaacctg 
      accgtggac cgcttcctg atggtgtac gacctgcgc 
      atgatgcgc gccgtgacg cccatccag accgtccta 
      gaccctctg tacttgcgc tttttgccg tcggtgtcg 
      tcgcgcttg gccgtggtt tcggccctt ggtcacgcc 
      caaatttta gatacagtg gcctttgct gaacctcgg 
      ctctgtcta atgcagatg gacagtcct gcaatgtgt 
      ctaaccttt gacatcagc gccaccagt caggcccta 
      gctttcggg gacagtacc ggctcgatt cacttgttt 
      tcgtcaacg gcccagccg ttgttcaat tcctattcg 
      cggatgacc gaacatggg gatccccta gttacgtac 
      cccagtttt gcgattgat gattttgag acgccgttg 
      gctgtgatt cccatgccg atttgtcac gacgggctg 
      tcgtcagac tggcccgaa catctgatg aaaaagtgc 
      tacaggcgt ccggtgggc gtagacccg gaggtgctg 
      cgcacgatg aagatgaag ggcccgatt ggctacgca 
      cccaatcca cgcaccacg aggagaaat caagtcatg 
      tacgagatt gataataac aaagcacga gcgcttaac 
      aaacacgat cgcaacgcg aaactggac gactctgaa 
      ggttttata gcgattccg aaacgttac cgcaaaatt 
      gacgtcaag tacaataaa ctgggcacg gatgagtgc 
      caatttgac cagtttaac aaaacaggt cacacggga 
      ctcgaggcg acgctaccc aacgcctac tgcaacgcc 
      atgattcaa gtgttgtat tacatcgag ccaatccga 
      gccgttctc ctgtcccat ttgtgcaat aaagaattt 
      tgtttatcg tgcgaattg ggtttcttg ttccacatg 
      ttgcgaaca tcatcgaac aattcgccc tgtcaaccg 
      gccaacttt ctacgagct tttcgcaca attcccgaa 
      gcttcggcc ttgagtctt gtctacagc gacctacca 
      cctgatcaa aaaacaaaa ataaatcta ggagctttg 
      attcagtct tggaaccgc ttcattatc caccaactg 
      cacgtcgaa cttgccgaa gccaaaaag aaaaacacc 
      gaacgaaaa aacaacaaa acgccgttt tcgtacaac 
      gtgaccgat tttcccgcc atagacgac cggaaaaag 
      tgttcagca tcagaagtt aatgcgaac atggagcag 
      gagaagcgc gacagtgag agtgaaatt atccaatta 
      ctgggcgcc aaacaaatc caaaaacac tcgtgtctc 
      aagtgcaaa ctcgaagta aaaaaagaa tcgatttta 
      ctcgtctgc aacctaatc tatccgacc aatgaaccg 
      gaacgtgac gaatggagt ttttgcgat attttaacg 
      cgttcgctt tgctcgcaa caaaccaca ccggcgtgg 
      tgcgaagag tgcaagaag ttcgcaccg acgcaacaa 
      acacgcgtg ctccagtcg cttcccaaa ctcttggcc 
      attaacacg ggcttgcac aacccgcag cataagcag 
      ttttggcaa agtcagatg gataaggtt gtggctaag 
      gtcacggaa aatgcagct ccgccaaca gcgccccag 
      attaatagt ctgagttcg tcaaagccg tgtcgttac 
      ggtgaccac tgttcgcgg ccagggtgt cgctttaga 
      cacagtttt gataatgtt ccgccccca gcacctgtt 
      aataatcca tactgtagt aacaattgg ctgccgcat 
      tcgatttcg attgcgctc aaagagggc gagttgatt 
      attgagaaa gttgaggat aagaaggaa gttgagggg 
      gaagagagg gttgaagat gagttgtat gatttaaca 
      gctgttgtt tgttatatt aatgatccg gcgtcgagt 
      gaaaaacgt aatattgtg gcgttgatt aaagtaccg 
      gattcgtat ttgtcggag acaacgcag tcggaacat 
      aagtggtat ctctttaat gattttagt atatcgaca 
      gtgtcgatg caagaagct gtgtggttc agtctggac 
      tggaagatt ccctgtatt ttgtactac tcttcgagg 
      gggattttg aaggcgaag tccgagatt tccccgccg 
      atttcaaag gaagtcttc actgaagac gcgtgcata 
      gcccgcagt tggggcacc tcagcaatc actttcaca 
      ccactaaca gacaacgaa ttccccaaa aagggcgac 
      ttagtcgca atggatgcg gaatttgtg acactaaac 
      caggaggag gctgagcta cgtagtgac ggcaaaatg 
      tcaatgata aaaccgtcg caaatgtcc gttgcgcga 
      attacatgc attcggggg tcgggcccc atggagggc 
      gtccctttc atcgacgac tatatttcc acgcaggaa 
      caagtggtt gactacttg accaaattc tcgggaatc 
      aaacccggg gatttggac gccaacttc agctcaaaa 
      cacctgacg actttaaag tccacgtac acaaaactg 
      cgctttttg ctcgacagt ggcgttatt ttcgtgggg 
      catgggctc aagaacgac tttagggtt attaactta 
      gtggtgcct cctgagcaa gttgccgac actgtgcat 
      ttgtttcac ctacctcat caccggatg gtgtcgctg 
      cgtttcctc gcgtggcat tttttgggt gttaaaata 
      cagtcggaa acgcacgat tcggtcgaa gatgcccga 
      gctgcgcta catttgtac aagaagtat aagcaactg 
      gacgctcaa agcaaggtg ggcgaggcg ctagccgag 
      ctgtatgag agaggcaag ctgctgaac tggaaggtg 
      cccgaggat tag
    mRNA sequence
    • >TC006714 RA
      gtcaattcc cagtccagt ttgaaatgt acaaagtaa 
      tttataagt taatatgta gcacaaata ctcataaac 
      gtcgttttt aatccctct ttttatttc tacacactt 
      tttaagcat gtttttcga ccaaagcgg gcttgcggt 
      ggcggcgcc gcccccgga cgtgcccga actgatggg 
      gatcgagtc agaatacgt ccaaacgca gtgtattct 
      agcggatgg gggcgaccg attcggggt gagcgccct 
      ggcgtttga ccgctatga ggagctcct ctggatggg 
      caaccaggg cggccacgt cacgtccta ctacaccgg 
      cgccatgca gaaatacac ctcgttcca aatccacgc 
      gactgaaga cgtgcgaca ggtacttac aataaacga 
      gggggtttt agggctaac ttccagtag tctaaggta 
      tcagataag gcgcgggat ccccgtctt cacccacac 
      ttctgccaa catgtacga aatgcaatg tatgctaca 
      agtgggggc gacgaggct cctgatggg ggggcacca 
      cggcaaact gatcgattt taacatcga tttgtgcaa 
      ggaaatgca agttgttga tgtggggga gcctggctg 
      cgttatgtt gcgccccca tagtaggtt tgtttgcgc 
      ggggaaccc cttgggcag gattgacct gagggaccc 
      caacacact caacactga acatacgct agagacgca 
      cagtggcag tctgagcga ttttgacgt ccaggggaa 
      ccttctggt gacttgtgg cttcagcaa taggcacgg 
      caacctgac cgtggaccg cttcctgat ggtgtacga 
      cctgcgcat gatgcgcgc cgtgacgcc catccagac 
      cgtcctaga ccctctgta cttgcgctt tttgccgtc 
      ggtgtcgtc gcgcttggc cgtggtttc ggcccttgg 
      tcacgccca aattttaga tacagtggc ctttgctga 
      acctcggct ctgtctaat gcagatgga cagtcctgc 
      aatgtgtct aacctttga catcagcgc caccagtca 
      ggccctagc tttcgggga cagtaccgg ctcgattca 
      cttgttttc gtcaacggc ccagccgtt gttcaattc 
      ctattcgcg gatgaccga acatgggga tcccctagt 
      tacgtaccc cagttttgc gattgatga ttttgagac 
      gccgttggc tgtgattcc catgccgat ttgtcacga 
      cgggctgtc gtcagactg gcccgaaca tctgatgaa 
      aaagtgcta caggcgtcc ggtgggcgt agacccgga 
      ggtgctgcg cacgatgaa gatgaaggg cccgattgg 
      ctacgcacc caatccacg caccacgag gagaaatca 
      agtcatgta cgagattga taataacaa agcacgagc 
      gcttaacaa acacgatcg caacgcgaa actggacga 
      ctctgaagg ttttatagc gattccgaa acgttaccg 
      caaaattga cgtcaagta caataaact gggcacgga 
      tgagtgcca atttgacca gtttaacaa aacaggtca 
      cacgggact cgaggcgac gctacccaa cgcctactg 
      caacgccat gattcaagt gttgtatta catcgagcc 
      aatccgagc cgttctcct gtcccattt gtgcaataa 
      agaattttg tttatcgtg cgaattggg tttcttgtt 
      ccacatgtt gcgaacatc atcgaacaa ttcgccctg 
      tcaaccggc caactttct acgagcttt tcgcacaat 
      tcccgaagc ttcggcctt gagtcttgt ctacagcga 
      cctaccacc tgatcaaaa aacaaaaat aaatctagg 
      agctttgat tcagtcttg gaaccgctt cattatcca 
      ccaactgca cgtcgaact tgccgaagc caaaaagaa 
      aaacaccga acgaaaaaa caacaaaac gccgttttc 
      gtacaacgt gaccgattt tcccgccat agacgaccg 
      gaaaaagtg ttcagcatc agaagttaa tgcgaacat 
      ggagcagga gaagcgcga cagtgagag tgaaattat 
      ccaattact gggcgccaa acaaatcca aaaacactc 
      gtgtctcaa gtgcaaact cgaagtaaa aaaagaatc 
      gattttact cgtctgcaa cctaatcta tccgaccaa 
      tgaaccgga acgtgacga atggagttt ttgcgatat 
      tttaacgcg ttcgctttg ctcgcaaca aaccacacc 
      ggcgtggtg cgaagagtg caagaagtt cgcaccgac 
      gcaacaaac acgcgtgct ccagtcgct tcccaaact 
      cttggccat taacacggg cttgcacaa cccgcagca 
      taagcagtt ttggcaaag tcagatgga taaggttgt 
      ggctaaggt cacggaaaa tgcagctcc gccaacagc 
      gccccagat taatagtct gagttcgtc aaagccgtg 
      tcgttacgg tgaccactg ttcgcggcc agggtgtcg 
      ctttagaca cagttttga taatgttcc gcccccagc 
      acctgttaa taatccata ctgtagtaa caattggct 
      gccgcattc gatttcgat tgcgctcaa agagggcga 
      gttgattat tgagaaagt tgaggataa gaaggaagt 
      tgaggggga agagagggt tgaagatga gttgtatga 
      tttaacagc tgttgtttg ttatattaa tgatccggc 
      gtcgagtga aaaacgtaa tattgtggc gttgattaa 
      agtaccgga ttcgtattt gtcggagac aacgcagtc 
      ggaacataa gtggtatct ctttaatga ttttagtat 
      atcgacagt gtcgatgca agaagctgt gtggttcag 
      tctggactg gaagattcc ctgtatttt gtactactc 
      ttcgagggg gattttgaa ggcgaagtc cgagatttc 
      cccgccgat ttcaaagga agtcttcac tgaagacgc 
      gtgcatagc ccgcagttg gggcacctc agcaatcac 
      tttcacacc actaacaga caacgaatt ccccaaaaa 
      gggcgactt agtcgcaat ggatgcgga atttgtgac 
      actaaacca ggaggaggc tgagctacg tagtgacgg 
      caaaatgtc aatgataaa accgtcgca aatgtccgt 
      tgcgcgaat tacatgcat tcgggggtc gggccccat 
      ggagggcgt ccctttcat cgacgacta tatttccac 
      gcaggaaca agtggttga ctacttgac caaattctc 
      gggaatcaa acccgggga tttggacgc caacttcag 
      ctcaaaaca cctgacgac tttaaagtc cacgtacac 
      aaaactgcg ctttttgct cgacagtgg cgttatttt 
      cgtggggca tgggctcaa gaacgactt tagggttat 
      taacttagt ggtgcctcc tgagcaagt tgccgacac 
      tgtgcattt gtttcacct acctcatca ccggatggt 
      gtcgctgcg tttcctcgc gtggcattt tttgggtgt 
      taaaataca gtcggaaac gcacgattc ggtcgaaga 
      tgcccgagc tgcgctaca tttgtacaa gaagtataa 
      gcaactgga cgctcaaag caaggtggg cgaggcgct 
      agccgagct gtatgagag aggcaagct gctgaactg 
      gaaggtgcc cgaggatta gacgagcct taattgtca 
      tgtgttgta cttttttat aaagtttta cgtctattt 
      gcagttttt taacgtgtt gtatcattc cagtctcgt 
      ttgtcaagg tgaaataaa aacagtgta aattatttt 
      ttcaaaata aattacttt tatatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY