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Gene information
General information
TC006700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13066357..13070301
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006700
    iBeetle screen iB_01082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    High number of defect larvae. All kind of defects, including many head defects. No pattern detectable, looks as if the amount of typical overall backround has increased.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006700 RA
      MNAFHVVVI FLMVRALSS QTVTCKSPI FCQGDLLRV 
      VQMAGIFKD SKTFVDMSL VRTMEKTLK NFEIMMTRT 
      GGSPNKNDV VEFIQKNFQ ASHNELDNW YPTDYQVNP 
      PFLRKIKDV RIRAIAKKL LDIWPNLGR KIKPEVMQN 
      PFKYSIIPV PNGFIVPGG RFREIYYWD SYWIMKGLL 
      ICDMKHTVK GMIDNFFRI IDMYGFIPN GGRIYYLDR 
      SQPPLLTLM VADFLEVSK EVLWLRNNM KYLEKELMY 
      WLTMKTVQV EKHGQKYRL ARYRSSDNT PRPESYIED 
      IHTASRYRT EDEKRQCYQ NIRTGAETG WDFSGRWFF 
      DHQGGNRAD LSTINPTRV IPVDLNAFL CRCFKIMHE 
      FYKKFRNHE RAAFWLQKY NEWLKAIRD VLYNVEDGI 
      WYDYDIELR VQRKHFYPS NFAPLWTEC YDTSIRQSY 
      GKNATNYLV QKGILNYEG GIPTSLFET GEQWDMPNA 
      WAPTQAIVI FGLDKSQDP GAKKVAQDL AFRWIDSLV 
      KVAEDTHEM FEKYNAMFK GMYGGGGEY EVQTGFGWT 
      NGVMLELIE HYYIKNKGA NTTET
    CDS sequence
    • >TC006700 RA
      atgaacgcg tttcatgtc gttgtgatt ttcctaatg 
      gtccgagcg ctaagtagt cagaccgtg acgtgcaaa 
      agtccgatc ttctgccag ggggatttg ctgcgcgtg 
      gtccaaatg gccgggatc ttcaaggac tcgaaaacg 
      ttcgttgat atgagtctg gtcaggacg atggaaaag 
      acgctcaaa aatttcgag attatgatg accagaacc 
      gggggaagt ccgaacaag aacgatgtt gttgagttc 
      atccagaag aacttccaa gcgtcgcat aatgagctg 
      gacaactgg tacccgacc gactaccag gtgaacccg 
      ccctttttg cgcaaaatc aaggatgtg aggattagg 
      gcgatcgcg aagaaattg ctggacatt tggccgaat 
      ctgggtcgg aagatcaag cccgaagtg atgcagaac 
      cccttcaag tactccatc atcccggtg cccaatggg 
      tttatcgtg cctgggggg cggttccgg gaaatctac 
      tactgggac tcgtactgg atcatgaag gggttgctg 
      atttgcgat atgaaacac accgtgaag gggatgatt 
      gataatttc ttccggatt attgacatg tatgggttt 
      atcccgaac ggggggaga atctactac ttggaccgg 
      tcgcaaccg ccactttta acactcatg gtggccgac 
      tttttggaa gtttcgaag gaagtgttg tggttgagg 
      aacaatatg aaatacttg gagaaggaa ctaatgtat 
      tggttgact atgaagacg gtccaggtg gaaaaacac 
      gggcagaag tatcggttg gctcggtac cgctccagt 
      gataatacg ccacgtcct gagtcctac atcgaggac 
      atacacaca gcctccagg taccgcacc gaagacgag 
      aagcgtcaa tgctaccag aacatccga acgggggcc 
      gagaccggc tgggacttc tccggccgt tggttcttc 
      gaccaccag ggcggcaac cgcgccgac ttgagcaca 
      atcaacccc actcgcgtc atccccgtg gacttgaac 
      gcgtttctg tgccgctgc ttcaaaatc atgcacgaa 
      ttctacaag aaattccgc aaccacgaa cgggctgcc 
      ttctggctg cagaagtac aacgagtgg ctgaaagcc 
      atcagagac gtgctgtac aacgtggag gacggcatc 
      tggtacgat tacgatatc gagctcaga gtccaaaga 
      aaacatttc tatcccagc aactttgcg ccgctctgg 
      actgagtgt tacgacacc agcatcagg caaagttac 
      gggaaaaac gccaccaat tatctggtc cagaagggg 
      attctcaac tacgaaggg gggattccc acgtccctg 
      tttgagaca ggggaacag tgggacatg ccaaatgcg 
      tgggccccc acacaggca attgtgatt tttgggctg 
      gataagagc caggatcct ggagcgaag aaggtggcc 
      caggacttg gcgtttcgc tggattgat tcgttggtt 
      aaagtggca gaagacacc catgagatg ttcgagaag 
      tacaacgcc atgttcaag gggatgtat gggggtggg 
      ggcgagtat gaggtccag acggggttt gggtggact 
      aatggggtc atgttggag cttattgag cactattat 
      attaagaac aaaggggcg aacaccaca gagacgtaa
    mRNA sequence
    • >TC006700 RA
      aaaaatgcc tgtcccgcc cgaaatcca gtgtcatga 
      acgcgtttc atgtcgttg tgattttcc taatggtcc 
      gagcgctaa gtagtcaga ccgtgacgt gcaaaagtc 
      cgatcttct gccaggggg atttgctgc gcgtggtcc 
      aaatggccg ggatcttca aggactcga aaacgttcg 
      ttgatatga gtctggtca ggacgatgg aaaagacgc 
      tcaaaaatt tcgagatta tgatgacca gaaccgggg 
      gaagtccga acaagaacg atgttgttg agttcatcc 
      agaagaact tccaagcgt cgcataatg agctggaca 
      actggtacc cgaccgact accaggtga acccgccct 
      ttttgcgca aaatcaagg atgtgagga ttagggcga 
      tcgcgaaga aattgctgg acatttggc cgaatctgg 
      gtcggaaga tcaagcccg aagtgatgc agaacccct 
      tcaagtact ccatcatcc cggtgccca atgggttta 
      tcgtgcctg gggggcggt tccgggaaa tctactact 
      gggactcgt actggatca tgaaggggt tgctgattt 
      gcgatatga aacacaccg tgaagggga tgattgata 
      atttcttcc ggattattg acatgtatg ggtttatcc 
      cgaacgggg ggagaatct actacttgg accggtcgc 
      aaccgccac ttttaacac tcatggtgg ccgactttt 
      tggaagttt cgaaggaag tgttgtggt tgaggaaca 
      atatgaaat acttggaga aggaactaa tgtattggt 
      tgactatga agacggtcc aggtggaaa aacacgggc 
      agaagtatc ggttggctc ggtaccgct ccagtgata 
      atacgccac gtcctgagt cctacatcg aggacatac 
      acacagcct ccaggtacc gcaccgaag acgagaagc 
      gtcaatgct accagaaca tccgaacgg gggccgaga 
      ccggctggg acttctccg gccgttggt tcttcgacc 
      accagggcg gcaaccgcg ccgacttga gcacaatca 
      accccactc gcgtcatcc ccgtggact tgaacgcgt 
      ttctgtgcc gctgcttca aaatcatgc acgaattct 
      acaagaaat tccgcaacc acgaacggg ctgccttct 
      ggctgcaga agtacaacg agtggctga aagccatca 
      gagacgtgc tgtacaacg tggaggacg gcatctggt 
      acgattacg atatcgagc tcagagtcc aaagaaaac 
      atttctatc ccagcaact ttgcgccgc tctggactg 
      agtgttacg acaccagca tcaggcaaa gttacggga 
      aaaacgcca ccaattatc tggtccaga aggggattc 
      tcaactacg aagggggga ttcccacgt ccctgtttg 
      agacagggg aacagtggg acatgccaa atgcgtggg 
      cccccacac aggcaattg tgatttttg ggctggata 
      agagccagg atcctggag cgaagaagg tggcccagg 
      acttggcgt ttcgctgga ttgattcgt tggttaaag 
      tggcagaag acacccatg agatgttcg agaagtaca 
      acgccatgt tcaagggga tgtatgggg gtgggggcg 
      agtatgagg tccagacgg ggtttgggt ggactaatg 
      gggtcatgt tggagctta ttgagcact attatatta 
      agaacaaag gggcgaaca ccacagaga cgtaataaa 
      tttcgttta gttggtagt ttttttctt gtattctta 
      gtcttacga gggaaaggt caacgtaag agcaaagca 
      aacaagtga gtttattgt tcccgtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY