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Gene information
General information
TC006699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13063527..13066301
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006699
    iBeetle screen iB_01081

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006699 RA
      MPFLLLLTL SFVTSEPCT SLIYCQGPL LHMVQTARI 
      FPDSKIFVD MKMQHDEKT TLENFHRFI KTTNKPNRK 
      EIKRFVNEN FKHFQEFDD WTPPDFRPN PDFLNRIRD 
      PEIRDFAHE VVQIWPKLS RKIKREVLE SAELFSLLP 
      VPNGFVVPG GRFREFYYW DSYWILKGL LLCQMHQTV 
      RGILDNFAF IIQKYGFLP NGARVYYLN RSQPPLLSL 
      MVGEYLRAT NDTEWLRKN IVWLDRELQ FFLENKLTT 
      LVKNGVKYQ LAHYVAKSD TPRPESYFE DIKSAKFLH 
      SPEARAELW TDLKSAAES GWDFSSRWM VDHDGGTKT 
      NLSHIQTRR IVPVDLNAF LCQAFGTLS GFYTTLGDH 
      KRALFWLQK SHLWRQNLE LVFYDHEDG VWYDWDRGL 
      NQPRKGYYA SNLTPLWTQ CYDPNLSDH LGQKAVQYL 
      SKTGILDFD GGIPASLVN SGEQWDFPN AWPPLQSIV 
      ILGLDHTGH PQAQKTAQD LAEKWIRSN LDSFKATGQ 
      ISEKYDVQF SGHSGRGGE YSVQHGFGW TNGVLLELI 
      DRYYGREK
    CDS sequence
    • >TC006699 RA
      atgcctttc ctcctactt ctcaccctc tcattcgtc 
      acaagcgag ccctgcacc agcctcatc tactgccaa 
      ggccccttg ctccacatg gtccaaacg gcgcgaatt 
      ttccccgac tcgaaaatc ttcgtcgac atgaaaatg 
      caacacgac gaaaaaacc accctcgaa aacttccac 
      cgtttcata aaaaccaca aacaaacca aaccgcaaa 
      gaaatcaaa cgcttcgtt aacgaaaac ttcaaacac 
      ttccaagag ttcgacgac tggaccccg cccgacttc 
      cggccaaac ccggatttt ttaaaccgg attcgcgac 
      ccggaaatc cgggatttt gcccacgaa gtggtccaa 
      atctggccg aaactctcc cggaagatc aaaagggag 
      gtgctggag tcggcggag ttgttctcg cttctgccg 
      gtaccgaac gggtttgtg gtacccgga gggcggttc 
      cgggagttc tactactgg gactcgtac tggattctg 
      aaggggctg ctcttgtgc cagatgcac cagactgtg 
      agggggatt ttggacaat ttcgccttc attatccag 
      aaatatggg tttttgcca aacggcgcc cgggtctat 
      tacttgaac cgctcccag cccccgctt ctgagcctc 
      atggttggg gagtactta cgtgcaaca aatgacacg 
      gagtggttg cggaagaac atagtttgg ctggaccgg 
      gagttgcaa tttttcctc gaaaataaa ctaacaact 
      ttggtaaaa aacggagtt aagtaccaa ctagcccat 
      tatgttgcc aaaagtgat acaccgcgt ccagagtcg 
      tactttgaa gacatcaaa agtgcgaaa tttttgcac 
      agtccggaa gccagggct gagttatgg accgatctg 
      aaaagtgcg gctgaaagt ggctgggat ttttcctcc 
      cgctggatg gttgaccac gatggtggt accaagacc 
      aacttgagc cacatccag accagacga atagtccca 
      gtggacttg aacgccttc ctctgccag gctttcggg 
      acattgtca ggtttttac accactttg ggtgaccac 
      aaaagggcg cttttctgg ctccaaaag tcacacttg 
      tggcgccaa aacctggaa ttggtgttt tatgaccac 
      gaggatggg gtttggtac gactgggac cgtgggtta 
      aaccaacca cggaagggt tactacgcc agtaattta 
      accccactg tggacacaa tgctacgac cccaacctt 
      tcggatcat ttgggccag aaagcagtc cagtacttg 
      tcaaaaacc gggattctg gactttgat ggtggcatc 
      cctgcctcc cttgtgaac agtggggag caatgggac 
      ttccccaac gcctggccg cccctccag tcaatcgtc 
      attctgggc ttggaccac acaggccac ccccaagcc 
      cagaaaacc gcccaagat ttggccgag aagtggatt 
      aggtcaaat ttggacagt ttcaaggcc acgggccaa 
      atttccgag aagtacgac gttcagttc agcggccac 
      agcggccgt ggcggggag tacagcgtc cagcacggc 
      ttcggctgg accaacggt gtactttta gagcttata 
      gaccggtac tacggccgg gaaaaataa
    mRNA sequence
    • >TC006699 RA
      atatttttg ttttttatc acttttaaa aggttttaa 
      aacacgaaa tcaagattt gactaaaat gcctttcct 
      cctacttct caccctctc attcgtcac aagcgagcc 
      ctgcaccag cctcatcta ctgccaagg ccccttgct 
      ccacatggt ccaaacggc gcgaatttt ccccgactc 
      gaaaatctt cgtcgacat gaaaatgca acacgacga 
      aaaaaccac cctcgaaaa cttccaccg tttcataaa 
      aaccacaaa caaaccaaa ccgcaaaga aatcaaacg 
      cttcgttaa cgaaaactt caaacactt ccaagagtt 
      cgacgactg gaccccgcc cgacttccg gccaaaccc 
      ggatttttt aaaccggat tcgcgaccc ggaaatccg 
      ggattttgc ccacgaagt ggtccaaat ctggccgaa 
      actctcccg gaagatcaa aagggaggt gctggagtc 
      ggcggagtt gttctcgct tctgccggt accgaacgg 
      gtttgtggt acccggagg gcggttccg ggagttcta 
      ctactggga ctcgtactg gattctgaa ggggctgct 
      cttgtgcca gatgcacca gactgtgag ggggatttt 
      ggacaattt cgccttcat tatccagaa atatgggtt 
      tttgccaaa cggcgcccg ggtctatta cttgaaccg 
      ctcccagcc cccgcttct gagcctcat ggttgggga 
      gtacttacg tgcaacaaa tgacacgga gtggttgcg 
      gaagaacat agtttggct ggaccggga gttgcaatt 
      tttcctcga aaataaact aacaacttt ggtaaaaaa 
      cggagttaa gtaccaact agcccatta tgttgccaa 
      aagtgatac accgcgtcc agagtcgta ctttgaaga 
      catcaaaag tgcgaaatt tttgcacag tccggaagc 
      cagggctga gttatggac cgatctgaa aagtgcggc 
      tgaaagtgg ctgggattt ttcctcccg ctggatggt 
      tgaccacga tggtggtac caagaccaa cttgagcca 
      catccagac cagacgaat agtcccagt ggacttgaa 
      cgccttcct ctgccaggc tttcgggac attgtcagg 
      tttttacac cactttggg tgaccacaa aagggcgct 
      tttctggct ccaaaagtc acacttgtg gcgccaaaa 
      cctggaatt ggtgtttta tgaccacga ggatggggt 
      ttggtacga ctgggaccg tgggttaaa ccaaccacg 
      gaagggtta ctacgccag taatttaac cccactgtg 
      gacacaatg ctacgaccc caacctttc ggatcattt 
      gggccagaa agcagtcca gtacttgtc aaaaaccgg 
      gattctgga ctttgatgg tggcatccc tgcctccct 
      tgtgaacag tggggagca atgggactt ccccaacgc 
      ctggccgcc cctccagtc aatcgtcat tctgggctt 
      ggaccacac aggccaccc ccaagccca gaaaaccgc 
      ccaagattt ggccgagaa gtggattag gtcaaattt 
      ggacagttt caaggccac gggccaaat ttccgagaa 
      gtacgacgt tcagttcag cggccacag cggccgtgg 
      cggggagta cagcgtcca gcacggctt cggctggac 
      caacggtgt acttttaga gcttataga ccggtacta 
      cggccggga aaaataaat tttttgagt attttttga 
      gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY