Status
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Gene information
General information
TC034167
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006696,TC006694
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12975074..13043728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034167
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01080

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034167 RA
      MFRGGMKLK ERFILGVSV AAVLFTFLL VVDIQMDLG 
      MSGHHLVPS HGRVKYVQE DAAGSAYNS FRKRFLQKT 
      HSASSTNAS KEVDAAPHS LKELPEKKP APSKEPKEK 
      HDDFSDLMD YMMLDSAER EKHRVEIDR VHPVIQKSM 
      EGDLVKVGN PTIAQLRKV AVRENATAL EKFHLQISQ 
      HELYPADSE LVDELIHEM ATEPILHVS QKSGGTQLK 
      LVIDYPNHL QALFKPMRF PREQQTLPN HFYFTDYER 
      HNAEIAAFH LDRLLGFRR AMPVTGRLL NMTTELYQK 
      AEGDLLKTF FVSPSDNLC FHGKCTYYC DTSHAICGN 
      PDSLEGSFA AFLPSNDVA ARKVWRHPW RRSYHKRRK 
      AQWETEDNY CALVREIPP YDRGRRLYD LMDMAVLDF 
      LMGNMDRHH YETFRLFGN DTFPLHLDH GRGFGRPFH 
      DELSILAPI LQCCMLRQT TLQTLMKFH NGPKLSEAM 
      RESLGRDPV APVLWEPHL EALDRRVEI ILRGIRDCL 
      QKGEDLGSE PGDEDT
    CDS sequence
    • >TC034167 RA
      atgttccga ggcgggatg aaattaaag gaacggttt 
      atactggga gtgagtgtg gcggcggtt ctgttcacg 
      ttcctcctg gtggtggac atccagatg gacctgggg 
      atgtcgggg caccacctg gtgccctcg cacgggagg 
      gtcaagtac gtgcaggag gatgcggcg gggtcggcc 
      tacaacagc ttcaggaag aggttccta caaaagaca 
      cacagcgcg agcagtaca aacgcttcg aaagaagtg 
      gacgcggct ccgcacagc ctgaaagag ctccctgaa 
      aaaaagccc gctcccagc aaagaaccg aaagaaaag 
      cacgacgat ttcagcgac cttatggac tacatgatg 
      ttggacagc gctgaaagg gagaaacat cgcgttgaa 
      attgatcga gtgcatccc gtcatccag aaaagcatg 
      gaaggggac ctggtcaag gtgggcaac ccgaccatt 
      gcccagctg cgcaaagtc gccgtaagg gagaacgcg 
      accgccttg gagaagttc catctgcaa atttcacaa 
      catgaattg tatccagct gacagcgag cttgtggat 
      gagcttata cacgaaatg gcgacagag cccattctg 
      catgtctcg caaaaaagc ggcggcact caactgaag 
      ctggtgata gattaccct aaccatctt caggcgttg 
      tttaagccg atgaggttc ccgcgggag cagcagacc 
      cttcccaat cacttttac ttcacggac tacgagcgc 
      cacaatgcc gagattgct gccttccac ttggacagg 
      cttttgggc ttccgaagg gccatgcct gtgacaggg 
      cgactgctc aacatgacc acagagctg taccaaaag 
      gccgagggg gatttactc aagacgttc ttcgtttcg 
      ccgtccgat aacctgtgc tttcatggc aagtgcacc 
      tattattgt gatacttca cacgccatc tgcggaaat 
      cccgacagt ttggaagga agctttgcc gccttcctg 
      ccctccaac gacgtcgcc gccaggaag gtatggaga 
      cacccttgg cgcagatct taccataaa cgaaggaaa 
      gctcaatgg gagactgaa gacaattat tgtgcgtta 
      gttagggag atcccgccg tacgaccga ggccggagg 
      ctttacgac ctcatggat atggccgta ttggacttt 
      ctaatgggc aatatggat agacaccat tacgagaca 
      tttagattg ttcggaaac gacacgttt ccgctccac 
      ctggaccat ggcaggggc ttcgggagg cccttccac 
      gacgagctg tccatccta gcccccatt ctgcagtgc 
      tgcatgctg aggcagacc accctccag acgctcatg 
      aaatttcac aacgggccg aagctgagc gaggccatg 
      agggagagc ttgggccgg gacccagtg gcgcccgtg 
      ctgtgggag ccccacttg gaggcactt gaccgccgc 
      gtggagatc atccttcga ggaatcagg gactgcctc 
      cagaaaggg gaggatttg ggctcggag ccgggagat 
      gaagacacg tag
    mRNA sequence
    • >TC034167 RA
      atgcaaaca aacttcagt catgtgctg aaagtcgcg 
      agttgtggg tgtgcgttc accgtctga aaacacgcg 
      aataaataa acaaaaacg acacgtgac gaggtgcga 
      gcgatattt tgtgcgaaa tgctgcgat ttcaggagg 
      acttccggt agcagcagg tgagtagta ccctggagg 
      gcgtccccg cccgcgggc tgccatgtg ccagcggcg 
      cccctcgac cccccacat tccgccgcc tcgaaagct 
      cccaacacc cccaagccg ccgccgccc ccgaaggct 
      acatccacg agcactaca gcccgaaaa gtggccgaa 
      aattcgacg agacgcggc agatcttca atcgatcac 
      cttgtccga tcaatcaag ataagggca ggcttatag 
      taccgccgc ctccgggcc cccggctca gtaccgcag 
      cggccgccg cggaccaag ctcaagtac tcgccgccg 
      ccggagccg aaaaccgct gagctatct gaaaaaatc 
      gaacagtgc ggcaagaat aatagcgtg cagtgtgtg 
      cgtgtcaat atgttccga ggcgggatg aaattaaag 
      gaacggttt atactggga gtgagtgtg gcggcggtt 
      ctgttcacg ttcctcctg gtggtggac atccagatg 
      gacctgggg atgtcgggg caccacctg gtgccctcg 
      cacgggagg gtcaagtac gtgcaggag gatgcggcg 
      gggtcggcc tacaacagc ttcaggaag aggttccta 
      caaaagaca cacagcgcg agcagtaca aacgcttcg 
      aaagaagtg gacgcggct ccgcacagc ctgaaagag 
      ctccctgaa aaaaagccc gctcccagc aaagaaccg 
      aaagaaaag cacgacgat ttcagcgac cttatggac 
      tacatgatg ttggacagc gctgaaagg gagaaacat 
      cgcgttgaa attgatcga gtgcatccc gtcatccag 
      aaaagcatg gaaggggac ctggtcaag gtgggcaac 
      ccgaccatt gcccagctg cgcaaagtc gccgtaagg 
      gagaacgcg accgccttg gagaagttc catctgcaa 
      atttcacaa catgaattg tatccagct gacagcgag 
      cttgtggat gagcttata cacgaaatg gcgacagag 
      cccattctg catgtctcg caaaaaagc ggcggcact 
      caactgaag ctggtgata gattaccct aaccatctt 
      caggcgttg tttaagccg atgaggttc ccgcgggag 
      cagcagacc cttcccaat cacttttac ttcacggac 
      tacgagcgc cacaatgcc gagattgct gccttccac 
      ttggacagg cttttgggc ttccgaagg gccatgcct 
      gtgacaggg cgactgctc aacatgacc acagagctg 
      taccaaaag gccgagggg gatttactc aagacgttc 
      ttcgtttcg ccgtccgat aacctgtgc tttcatggc 
      aagtgcacc tattattgt gatacttca cacgccatc 
      tgcggaaat cccgacagt ttggaagga agctttgcc 
      gccttcctg ccctccaac gacgtcgcc gccaggaag 
      gtatggaga cacccttgg cgcagatct taccataaa 
      cgaaggaaa gctcaatgg gagactgaa gacaattat 
      tgtgcgtta gttagggag atcccgccg tacgaccga 
      ggccggagg ctttacgac ctcatggat atggccgta 
      ttggacttt ctaatgggc aatatggat agacaccat 
      tacgagaca tttagattg ttcggaaac gacacgttt 
      ccgctccac ctggaccat ggcaggggc ttcgggagg 
      cccttccac gacgagctg tccatccta gcccccatt 
      ctgcagtgc tgcatgctg aggcagacc accctccag 
      acgctcatg aaatttcac aacgggccg aagctgagc 
      gaggccatg agggagagc ttgggccgg gacccagtg 
      gcgcccgtg ctgtgggag ccccacttg gaggcactt 
      gaccgccgc gtggagatc atccttcga ggaatcagg 
      gactgcctc cagaaaggg gaggatttg ggctcggag 
      ccgggagat gaagacacg tagtaacga cttgtacaa 
      taataatgt ataaatcct tgtaaagct gtgacagag 
      cgagattca tgtaaatat tttatccgt taccaaata 
      tgttttagt cacttttag accaaaaag ctagtttgt 
      acatacttg ttgtaaatt aatttgtat aaatatttt 
      gataagggt gtcgaataa tgttgtacg attttcctt 
      ggggtgcgg acacgtgct cataattat accaaagct 
      gactgtatt tgcaatgtg atgaataaa gttacgtaa 
      gactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY