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Gene information
General information
TC006685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1400172..1403442
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006685
      iBeetle screen iB_01078
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Fertility
      eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection



      Adult morphology
      elytra not closed - Number of animals showing the phenotype: 3


      hindwing irregular - Number of animals showing the phenotype: 3

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006685 RA
        MSFGHALIF LCLLLPAFA APDLPHTLA RISEARARA 
        IANRARLRA NSARVINQV RNNRSKRQD QHFDNEEPT 
        FVHICPMDD ESQARSAED SGETLPEGP SNEENHLDP 
        SSSLNQALD ELMEARRLL DRFEGQRDA EAYRHFHVH 
        QHSTIHGHG QLPLPTTQV LHPGLPETH GLNLNFHQT 
        QGLYPGLPI VNHGVVPSQ IAPIHPGMG VFQTRLREN 
        DQDDDFPGP MIDFDEEPG DMDEQLDNE LSPFDQFSG 
        EREANPTLH HKKMKLKQK LNQKFNNYG PPPQYYGHY 
        REAEEREAS PTLHKKRKL KQKLNQKFG QGYPQYYVP 
        YREVEEEGE EEEEEREAS PTLHKKRKL KQKLNQKFG 
        QGQAYPQVQ YYREAEPQQ PGDLFQDTI TSPYLFDVP 
        NRRFKDYRR SADQDLDAL LGVQRQIID TMKEKAQND 
        QDLDFLTDI SNRTLSSIE DRRKKISKR SIPLTRLIS 
        DPNVRLDVP KTFENFGTA TKHAFEDER APMYHLKHL 
        VGSSKNALL SAMRIPPQN MIIPSDYEV VRASTRGGS 
        TREDDSFGN TFKRVGEMV RESIRSGQE TVAHISDAA 
        HEARNMLNT ARHGAPRLI IPYSQIPAL HKGPRKAVL 
        ITPRILDLA EDHTARKSS DLRPNFLRL GHLMDAVGF 
        SKPHAKNLD LEKIVFEPK RVHKYNDEI VEALNTLED 
        AKVSVGAKN LRDLLLKMR DELTNVVAE EPRKKHKSK 
        RKKQQETKE MGKFIETLF DGTQEERVG DMMLAPELL 
        RPFMGRASD SDLHNYLRE NGDETTPKK HFEELKNST 
        NPDLGYVLG AINPLMLER MFNQTVEVN SGRNETVGT 
        AMGLNGAML KPFMGQAQN QAVEDFLDT YNHDPNDDY 
        VEEIDE
      CDS sequence
      • >TC006685 RA
        atgagtttt gggcacgct ttaattttc ctgtgcctg 
        cttttgccc gctttcgcc gcccctgat ctccctcac 
        accttggca aggatcagc gaagctaga gctagagca 
        atcgcaaat cgggcgaga cttcgggca aactccgcc 
        agggtcata aatcaggtg cggaacaat cgctcaaaa 
        cgccaggac caacatttc gacaatgaa gaaccgact 
        tttgtccac atttgccca atggacgac gaaagccag 
        gccaggagt gccgaagac tcgggtgaa actctccca 
        gaggggcca tcaaatgag gaaaatcac ctggacccc 
        agctcttcg ctgaaccag gctctggac gagctgatg 
        gaagcccgg aggcttctg gaccggttt gagggacaa 
        agggacgca gaggcctat cgccacttc cacgtccac 
        caacactcc accatccat gggcatgga caattacct 
        ctaccaaca acccaggtt ttgcaccca ggcttgccc 
        gaaacgcat ggtcttaac ctaaacttt caccaaacc 
        cagggcctt tacccgggc ttgcccatc gtcaaccat 
        ggggtggta ccgtcccaa atcgcaccc atccatccc 
        gggatgggc gttttccag acgagactg cgggagaac 
        gaccaagat gatgatttt ccaggtccc atgattgat 
        tttgacgaa gaacctgga gatatggac gagcaactg 
        gacaacgaa ctttcgccg ttcgaccag ttctcgggc 
        gaacgtgag gcgaacccg actttgcac cacaaaaaa 
        atgaagttg aagcaaaag ttgaaccag aaatttaat 
        aattacgga cctccccca cagtattat ggtcactat 
        cgtgaagct gaagaacgt gaagctagt ccgactttg 
        cacaaaaaa cgcaaactg aagcaaaaa cttaaccag 
        aagtttggt caaggttac ccgcagtac tacgtccct 
        tatcgggaa gttgaagaa gaaggagaa gaagaagaa 
        gaagaaagg gaggctagt ccaactttg cacaaaaaa 
        cgcaaactg aagcaaaaa ctcaaccag aagtttggt 
        caaggtcag gcgtacccc caggtgcag tactaccgt 
        gaggccgaa ccccaacaa cctggggac cttttccaa 
        gacactatc acttcccct tatcttttc gacgtcccc 
        aaccggcgc ttcaaagac tacaggcgt tcagcggac 
        caggacctt gacgccctt ctgggcgtc cagcgccag 
        atcatcgac acgatgaag gagaaggcg caaaacgac 
        caagacttg gactttctg accgacatc agcaaccga 
        acactgagt tcaatcgaa gaccggcga aaaaaaatc 
        tcaaaacgt tccatcccc ctaacccgc ctaataagc 
        gacccgaac gtgcgtctg gacgtccca aagaccttc 
        gagaacttt ggcacggcc accaagcac gccttcgag 
        gatgagagg gcccccatg taccacctg aagcacctg 
        gtggggtcg tccaaaaac gccctgctc tccgccatg 
        cggatcccg ccccagaac atgatcata ccgtcggat 
        tacgaagtt gtgagggcg tcaacacgt gggggaagc 
        acccgtgaa gacgactct tttggcaac acgttcaag 
        cgagtgggg gagatggta agggagagt ataaggtcg 
        gggcaagaa acggtggcc cacatcagc gacgcggcc 
        cacgaagcc cgcaatatg ctcaacacg gcccgacac 
        ggagccccc agactcata atcccctat tcccaaatc 
        ccagccctt cacaaaggc ccccgtaag gcggttctg 
        ataactccc aggattctg gacctggct gaggaccac 
        accgcgcga aaatcaagt gacttgcga ccgaacttc 
        ctccgcctg ggccacttg atggacgcc gttggtttt 
        tccaaacca cacgcaaaa aatctggac ttggaaaaa 
        atcgttttc gagccgaaa cgtgtccat aaatacaac 
        gatgagata gttgaggct ttgaatacg ctggaagac 
        gccaaggtt tcagtgggc gccaaaaac ctgagggac 
        cttttgctc aaaatgcgc gacgagttg accaacgtg 
        gtggcggaa gagcccagg aagaagcac aagagtaag 
        aggaagaag cagcaagag accaaagaa atggggaag 
        tttatcgag actttgttc gacgggacg caggaggag 
        agagttggg gatatgatg cttgcgcct gagctgctg 
        cgccccttc atggggcgg gcgtccgac agcgacttg 
        cacaattat ttgcgcgaa aatggggat gaaacaacg 
        cccaaaaaa cacttcgag gagttgaaa aactcgact 
        aatcccgat ctggggtat gttttaggt gcaattaac 
        ccgcttatg ttggagagg atgtttaat cagacggtt 
        gaggtgaat tcggggcgg aatgagact gtggggacg 
        gccatgggg ttgaatggg gccatgctg aagccgttc 
        atggggcaa gcccagaac caggcagtt gaggatttt 
        ttagacacg tataaccat gaccccaac gatgattac 
        gtggaagaa atagacgag tag
      mRNA sequence
      • >TC006685 RA
        gattttagg tcccgacca atcagactt gtaattcgg 
        acacggacc agatctccg caacaccga agcgttatt 
        tttgctgtt ttgcttgcc gcagacgca ttttcattt 
        gacaaactc ttttatatg aaagattaa ataccccaa 
        tcggttctt tgtaaagtt aatcaagtt ttgcaagga 
        ttgttgttt tatttcaag ctacttagg taaatttgg 
        caacttgga caaagcact ttcttgctg tcgttgtaa 
        ataaagtgc ctgttattg tatacttac cactgtaac 
        atattttta aataaacat atttaatta gtctgcact 
        tttattgag cacccactt ttgcgaaac gtccctcag 
        taggtcagc acgattgct tcagcagca aatttcctc 
        tgacacggc aattcccag ccaactgtt cgtgcaagt 
        gccacgcga aatgagttt tgggcacgc tttaatttt 
        cctgtgcct gcttttgcc cgctttcgc cgcccctga 
        tctccctca caccttggc aaggatcag cgaagctag 
        agctagagc aatcgcaaa tcgggcgag acttcgggc 
        aaactccgc cagggtcat aaatcaggt gcggaacaa 
        tcgctcaaa acgccagga ccaacattt cgacaatga 
        agaaccgac ttttgtcca catttgccc aatggacga 
        cgaaagcca ggccaggag tgccgaaga ctcgggtga 
        aactctccc agaggggcc atcaaatga ggaaaatca 
        cctggaccc cagctcttc gctgaacca ggctctgga 
        cgagctgat ggaagcccg gaggcttct ggaccggtt 
        tgagggaca aagggacgc agaggccta tcgccactt 
        ccacgtcca ccaacactc caccatcca tgggcatgg 
        acaattacc tctaccaac aacccaggt tttgcaccc 
        aggcttgcc cgaaacgca tggtcttaa cctaaactt 
        tcaccaaac ccagggcct ttacccggg cttgcccat 
        cgtcaacca tggggtggt accgtccca aatcgcacc 
        catccatcc cgggatggg cgttttcca gacgagact 
        gcgggagaa cgaccaaga tgatgattt tccaggtcc 
        catgattga ttttgacga agaacctgg agatatgga 
        cgagcaact ggacaacga actttcgcc gttcgacca 
        gttctcggg cgaacgtga ggcgaaccc gactttgca 
        ccacaaaaa aatgaagtt gaagcaaaa gttgaacca 
        gaaatttaa taattacgg acctccccc acagtatta 
        tggtcacta tcgtgaagc tgaagaacg tgaagctag 
        tccgacttt gcacaaaaa acgcaaact gaagcaaaa 
        acttaacca gaagtttgg tcaaggtta cccgcagta 
        ctacgtccc ttatcggga agttgaaga agaaggaga 
        agaagaaga agaagaaag ggaggctag tccaacttt 
        gcacaaaaa acgcaaact gaagcaaaa actcaacca 
        gaagtttgg tcaaggtca ggcgtaccc ccaggtgca 
        gtactaccg tgaggccga accccaaca acctgggga 
        ccttttcca agacactat cacttcccc ttatctttt 
        cgacgtccc caaccggcg cttcaaaga ctacaggcg 
        ttcagcgga ccaggacct tgacgccct tctgggcgt 
        ccagcgcca gatcatcga cacgatgaa ggagaaggc 
        gcaaaacga ccaagactt ggactttct gaccgacat 
        cagcaaccg aacactgag ttcaatcga agaccggcg 
        aaaaaaaat ctcaaaacg ttccatccc cctaacccg 
        cctaataag cgacccgaa cgtgcgtct ggacgtccc 
        aaagacctt cgagaactt tggcacggc caccaagca 
        cgccttcga ggatgagag ggcccccat gtaccacct 
        gaagcacct ggtggggtc gtccaaaaa cgccctgct 
        ctccgccat gcggatccc gccccagaa catgatcat 
        accgtcgga ttacgaagt tgtgagggc gtcaacacg 
        tgggggaag cacccgtga agacgactc ttttggcaa 
        cacgttcaa gcgagtggg ggagatggt aagggagag 
        tataaggtc ggggcaaga aacggtggc ccacatcag 
        cgacgcggc ccacgaagc ccgcaatat gctcaacac 
        ggcccgaca cggagcccc cagactcat aatccccta 
        ttcccaaat cccagccct tcacaaagg cccccgtaa 
        ggcggttct gataactcc caggattct ggacctggc 
        tgaggacca caccgcgcg aaaatcaag tgacttgcg 
        accgaactt cctccgcct gggccactt gatggacgc 
        cgttggttt ttccaaacc acacgcaaa aaatctgga 
        cttggaaaa aatcgtttt cgagccgaa acgtgtcca 
        taaatacaa cgatgagat agttgaggc tttgaatac 
        gctggaaga cgccaaggt ttcagtggg cgccaaaaa 
        cctgaggga ccttttgct caaaatgcg cgacgagtt 
        gaccaacgt ggtggcgga agagcccag gaagaagca 
        caagagtaa gaggaagaa gcagcaaga gaccaaaga 
        aatggggaa gtttatcga gactttgtt cgacgggac 
        gcaggagga gagagttgg ggatatgat gcttgcgcc 
        tgagctgct gcgcccctt catggggcg ggcgtccga 
        cagcgactt gcacaatta tttgcgcga aaatgggga 
        tgaaacaac gcccaaaaa acacttcga ggagttgaa 
        aaactcgac taatcccga tctggggta tgttttagg 
        tgcaattaa cccgcttat gttggagag gatgtttaa 
        tcagacggt tgaggtgaa ttcggggcg gaatgagac 
        tgtggggac ggccatggg gttgaatgg ggccatgct 
        gaagccgtt catggggca agcccagaa ccaggcagt 
        tgaggattt tttagacac gtataacca tgaccccaa 
        cgatgatta cgtggaaga aatagacga gtagttttt 
        tttttattt tttatttat tttgtttag ggtttttta 
        caaaaaatg ttagttagt gaatctgat cctgttgta 
        agtggtgta acgggtgtt ttgatatgg tggtcttgg 
        tcttcggta caccggagc tgtaaaaat gaagtaatc 
        aataaagtt gttacgtaa tt