| General information | |||
| TC034160
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12708528..12713590 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034160 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01076 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034160 RA
MSSFYLYYT ACVANLIAF TVGLAYGLA SPLLPRLNG SVDPDNNPL DPPPTPSEE SLIASLLSL GAIFGPLLT GLFVDKIGR KKTLLIVAL PIIASFLTM AFAHSVVLY YIARLVMGV GAGSVFTVM PMYLGEISE DRNRGTLGC LMSTFIASG VLFDYAVGP FLTVQCYCL VCLAPLGAF LALFGGWAP ESPQFLALR GDEGRLRAC LAKLRGSRD VDKEIVGVR ESLKGAQGG LRELFQSRA SRKGLVITV GLMVLQQMA GINAVNSYL QTIFDATGS GLSPEISSI IIGTVQVFT TVLTSSLVD RSGRRILLL FSMVGSGVS LVSLGSYFY LKSNSFQVD TLSWLPVMS LVVFIISFN IGLGPIPWA VMAEMFPPN VKSIASTFS SIVCFIAAF TITLIFPSL AEVLGMGQA FCRIVLKTV TPISSPKAL TMAEPSLQE VVYKPTTDT PTPTALKPS TAPTTSAFL SFSAAIADL AAFSAGVAF GWPSPVLPK LAGHNNPLG RPITHTQAS WIAGLVCLG AILGPLLAG PVADKLGRK KALILAACP MTGSLLLAA YATTLPWFY LSRFAMGVG AGSVFTVLP IYLAEIAQD HNRGTLGCS MGAFVASGL LFAFAVGPF LEVGTFCLV CTLPLLVFL AVFSAFVPE SPFFLAAAN RSRDLEQSL MKLRNSENV GDEVLEITQ RVFEERKIK TGLLDLFKF RALRRGLVV TLGIVILQQ FAGINAVLS YLQTIFEAS GSGQSPEMA TIIIGVVQM VATVVTSLL ADRLGRRVL LLTSAVGSS VALLALGLY FYRKGQHLE VGAISWLPV ASLVVYMVA FNVGLGPLP WAVMGELFP SSVKSVAAG FTCFICFVA AFVITLLFP ILSNLVGMA NSFWFFAGM CLLGAFFIY WMLPETKGK SVQEIQKLL GGD
- >TC034160 RA
atgtccagt ttttacttg tactatacc gcgtgcgtg gcgaatttg attgcgttc actgtgggg cttgcctac gggttggcg tcccccctc ctcccgaga ctcaacgga agtgtggac cccgataac aacccactg gacccaccc ccaacccct tccgaggag tctctaatc gcgtcttta ctatcactt ggagctatt ttcggccca cttttaacc ggtctcttt gttgataaa atcgggcgg aaaaaaacg ctcctaatc gtggctttg cccataatt gcgtcattc ctgaccatg gctttcgcc cattcggtg gtactttac tacatcgcg cgtttggtc atgggtgtg ggggcgggg agcgtcttc acagtcatg cccatgtac ctgggggag atctccgag gaccgcaac cgcggcact ttggggtgt ctcatgtcg acgtttatc gcctctggg gtgcttttc gactacgcc gtggggccg tttttgacc gttcagtgc tactgtttg gtgtgtttg gcgcctctg ggggcgttt ttggcgctg tttgggggg tgggccccc gaatcgccc caattcctg gccttgagg ggcgatgag gggcgcttg agggcgtgt ttggccaag ttgcggggg tcgcgggac gttgataag gaaattgtc ggtgttagg gagagtctt aagggggct cagggcggt ttgagggag ttgtttcag tcaagggct agtcgcaaa gggttggtt ataaccgtc ggtttgatg gttttgcag caaatggct gggatcaac gctgtgaat tcgtacttg cagacgatt ttcgatgca acaggaagc gggctttca ccggaaatc tcctcgatt attatagga acggttcag gtcttcacc acggtgtta acatcgtcg cttgtggat agatcgggg cggaggatt cttctttta ttttcaatg gtcgggtca ggtgtttct cttgtttcg cttgggtcg tatttctac ctcaaaagc aacagtttc caagtcgat acactctcc tggcttcca gtaatgagt ttagttgtt tttattatc tcgtttaat atcggcttg gggccgatt ccgtgggcc gtcatggcg gagatgttt ccgccaaat gttaaatca attgcttcg actttctcc agtattgtg tgttttatt gcggctttt accatcact ttgattttt ccgtcgttg gctgaggtg ctgggcatg gggcaggcc ttctgtaga atagttctc aagacagtg acgccaata agttcccca aaagcgctc accatggcc gaaccgtct ctccaagaa gtggtctac aagcccacc accgacacc ccaaccccc accgctcta aaaccctcc accgccccc acaaccagc gccttcctc agcttttcc gccgcaatc gccgacctg gccgccttc tccgccggc gtggccttc ggctggcct tcccccgtc ctcccgaag ctagccggc cacaacaac ccattgggc cgccccatc acccacacc caagcctcc tggatcgcc gggctggtc tgcttgggc gccatcctg ggcccgctg ctcgccggc cccgtcgcc gacaaactg ggccgcaaa aaggcccta atcctggcc gcgtgtccc atgactggg tccctgctc ctggcggcg tacgccacc acactccca tggttctac ctctcccgg ttcgccatg ggcgtgggc gccggcagc gtcttcacg gtcctgccc atctacttg gccgaaatc gcccaggac cacaaccgc ggcacgctg ggctgctcc atgggcgcg tttgtggct tctgggctg ttgttcgcc ttcgcggtg ggtcccttt ctggaagtg gggacgttt tgtctcgtt tgtaccctc ccattgctg gttttcctg gccgttttt tcggctttt gtcccagaa agtcccttt tttctggcc gcggctaac cggagccgt gacttagag caaagtttg atgaaattg aggaacagt gaaaatgtt ggggacgaa gttttggaa atcacgcag cgggttttt gaggagagg aaaatcaaa actggcctt ctggatttg ttcaaattc cgggcgttg aggcgcggt ttggttgta actcttgga attgtgatc ctgcagcaa tttgcggga attaacgcc gttttgtcg tacttgcag acgattttt gaggcttcc ggaagtggg caaagcccg gaaatggcc acaattatc atcggggtt gtgcaaatg gtcgcaact gtggtcacg tccctcctg gctgatcgt ctaggccgc agggttctg ctgctgact tcggcagtc gggtcaagt gttgcgctc ctggctcta gggctgtat ttctaccgc aagggccag cacctggaa gttggggcg atttcctgg ctgccggtg gccagtttg gtcgtgtac atggtggcg tttaatgtg ggcttgggg ccactgccg tgggctgtc atgggcgag ctttttccc tcaagtgtc aagtcagtg gcggcgggt tttacgtgt tttatctgc ttcgtggcc gcgtttgtt atcactctg ttgttcccg attttgtcg aatcttgtg gggatggcg aattcgttc tggtttttc gcagggatg tgtctttta ggggcgttt tttatctat tggatgctg ccggagacc aagggcaag agtgtgcaa gaaatacaa aaattgtta gggggggat tga
- >TC034160 RA
gatttaata aatgtcccc atttaattt tcgcgtcat tatttaacc gaaaatgtc cagttttta cttgtacta taccgcgtg cgtggcgaa tttgattgc gttcactgt ggggcttgc ctacgggtt ggcgtcccc cctcctccc gagactcaa cggaagtgt ggaccccga taacaaccc actggaccc acccccaac cccttccga ggagtctct aatcgcgtc tttactatc acttggagc tattttcgg cccactttt aaccggtct ctttgttga taaaatcgg gcggaaaaa aacgctcct aatcgtggc tttgcccat aattgcgtc attcctgac catggcttt cgcccattc ggtggtact ttactacat cgcgcgttt ggtcatggg tgtgggggc ggggagcgt cttcacagt catgcccat gtacctggg ggagatctc cgaggaccg caaccgcgg cactttggg gtgtctcat gtcgacgtt tatcgcctc tggggtgct tttcgacta cgccgtggg gccgttttt gaccgttca gtgctactg tttggtgtg tttggcgcc tctgggggc gtttttggc gctgtttgg ggggtgggc ccccgaatc gccccaatt cctggcctt gaggggcga tgaggggcg cttgagggc gtgtttggc caagttgcg ggggtcgcg ggacgttga taaggaaat tgtcggtgt tagggagag tcttaaggg ggctcaggg cggtttgag ggagttgtt tcagtcaag ggctagtcg caaagggtt ggttataac cgtcggttt gatggtttt gcagcaaat ggctgggat caacgctgt gaattcgta cttgcagac gattttcga tgcaacagg aagcgggct ttcaccgga aatctcctc gattattat aggaacggt tcaggtctt caccacggt gttaacatc gtcgcttgt ggatagatc ggggcggag gattcttct tttattttc aatggtcgg gtcaggtgt ttctcttgt ttcgcttgg gtcgtattt ctacctcaa aagcaacag tttccaagt cgatacact ctcctggct tccagtaat gagtttagt tgtttttat tatctcgtt taatatcgg cttggggcc gattccgtg ggccgtcat ggcggagat gtttccgcc aaatgttaa atcaattgc ttcgacttt ctccagtat tgtgtgttt tattgcggc ttttaccat cactttgat ttttccgtc gttggctga ggtgctggg catggggca ggccttctg tagaatagt tctcaagac agtgacgcc aataagttc cccaaaagc gctcaccat ggccgaacc gtctctcca agaagtggt ctacaagcc caccaccga caccccaac ccccaccgc tctaaaacc ctccaccgc ccccacaac cagcgcctt cctcagctt ttccgccgc aatcgccga cctggccgc cttctccgc cggcgtggc cttcggctg gccttcccc cgtcctccc gaagctagc cggccacaa caacccatt gggccgccc catcaccca cacccaagc ctcctggat cgccgggct ggtctgctt gggcgccat cctgggccc gctgctcgc cggccccgt cgccgacaa actgggccg caaaaaggc cctaatcct ggccgcgtg tcccatgac tgggtccct gctcctggc ggcgtacgc caccacact cccatggtt ctacctctc ccggttcgc catgggcgt gggcgccgg cagcgtctt cacggtcct gcccatcta cttggccga aatcgccca ggaccacaa ccgcggcac gctgggctg ctccatggg cgcgtttgt ggcttctgg gctgttgtt cgccttcgc ggtgggtcc ctttctgga agtggggac gttttgtct cgtttgtac cctcccatt gctggtttt cctggccgt tttttcggc ttttgtccc agaaagtcc cttttttct ggccgcggc taaccggag ccgtgactt agagcaaag tttgatgaa attgaggaa cagtgaaaa tgttgggga cgaagtttt ggaaatcac gcagcgggt ttttgagga gaggaaaat caaaactgg ccttctgga tttgttcaa attccgggc gttgaggcg cggtttggt tgtaactct tggaattgt gatcctgca gcaatttgc gggaattaa cgccgtttt gtcgtactt gcagacgat ttttgaggc ttccggaag tgggcaaag cccggaaat ggccacaat tatcatcgg ggttgtgca aatggtcgc aactgtggt cacgtccct cctggctga tcgtctagg ccgcagggt tctgctgct gacttcggc agtcgggtc aagtgttgc gctcctggc tctagggct gtatttcta ccgcaaggg ccagcacct ggaagttgg ggcgatttc ctggctgcc ggtggccag tttggtcgt gtacatggt ggcgtttaa tgtgggctt ggggccact gccgtgggc tgtcatggg cgagctttt tccctcaag tgtcaagtc agtggcggc gggttttac gtgttttat ctgcttcgt ggccgcgtt tgttatcac tctgttgtt cccgatttt gtcgaatct tgtggggat ggcgaattc gttctggtt tttcgcagg gatgtgtct tttaggggc gttttttat ctattggat gctgccgga gaccaaggg caagagtgt gcaagaaat acaaaaatt gttaggggg ggattgatt gaataaaag cacttgcaa agt
