Status
Please wait ...
Gene information
General information
TC034160
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006667,TC006666 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12708528..12713590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034160
    iBeetle screen iB_01076

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034160 RA
      MSSFYLYYT ACVANLIAF TVGLAYGLA SPLLPRLNG 
SVDPDNNPL DPPPTPSEE SLIASLLSL GAIFGPLLT 
GLFVDKIGR KKTLLIVAL PIIASFLTM AFAHSVVLY 
YIARLVMGV GAGSVFTVM PMYLGEISE DRNRGTLGC 
LMSTFIASG VLFDYAVGP FLTVQCYCL VCLAPLGAF 
LALFGGWAP ESPQFLALR GDEGRLRAC LAKLRGSRD 
VDKEIVGVR ESLKGAQGG LRELFQSRA SRKGLVITV 
GLMVLQQMA GINAVNSYL QTIFDATGS GLSPEISSI 
IIGTVQVFT TVLTSSLVD RSGRRILLL FSMVGSGVS 
LVSLGSYFY LKSNSFQVD TLSWLPVMS LVVFIISFN 
IGLGPIPWA VMAEMFPPN VKSIASTFS SIVCFIAAF 
TITLIFPSL AEVLGMGQA FCRIVLKTV TPISSPKAL 
TMAEPSLQE VVYKPTTDT PTPTALKPS TAPTTSAFL 
SFSAAIADL AAFSAGVAF GWPSPVLPK LAGHNNPLG 
RPITHTQAS WIAGLVCLG AILGPLLAG PVADKLGRK 
KALILAACP MTGSLLLAA YATTLPWFY LSRFAMGVG 
AGSVFTVLP IYLAEIAQD HNRGTLGCS MGAFVASGL 
LFAFAVGPF LEVGTFCLV CTLPLLVFL AVFSAFVPE 
SPFFLAAAN RSRDLEQSL MKLRNSENV GDEVLEITQ 
RVFEERKIK TGLLDLFKF RALRRGLVV TLGIVILQQ 
FAGINAVLS YLQTIFEAS GSGQSPEMA TIIIGVVQM 
VATVVTSLL ADRLGRRVL LLTSAVGSS VALLALGLY 
FYRKGQHLE VGAISWLPV ASLVVYMVA FNVGLGPLP 
WAVMGELFP SSVKSVAAG FTCFICFVA AFVITLLFP 
ILSNLVGMA NSFWFFAGM CLLGAFFIY WMLPETKGK 
SVQEIQKLL GGD
    CDS sequence
    • >TC034160 RA
      atgtccagt ttttacttg tactatacc gcgtgcgtg 
gcgaatttg attgcgttc actgtgggg cttgcctac 
gggttggcg tcccccctc ctcccgaga ctcaacgga 
agtgtggac cccgataac aacccactg gacccaccc 
ccaacccct tccgaggag tctctaatc gcgtcttta 
ctatcactt ggagctatt ttcggccca cttttaacc 
ggtctcttt gttgataaa atcgggcgg aaaaaaacg 
ctcctaatc gtggctttg cccataatt gcgtcattc 
ctgaccatg gctttcgcc cattcggtg gtactttac 
tacatcgcg cgtttggtc atgggtgtg ggggcgggg 
agcgtcttc acagtcatg cccatgtac ctgggggag 
atctccgag gaccgcaac cgcggcact ttggggtgt 
ctcatgtcg acgtttatc gcctctggg gtgcttttc 
gactacgcc gtggggccg tttttgacc gttcagtgc 
tactgtttg gtgtgtttg gcgcctctg ggggcgttt 
ttggcgctg tttgggggg tgggccccc gaatcgccc 
caattcctg gccttgagg ggcgatgag gggcgcttg 
agggcgtgt ttggccaag ttgcggggg tcgcgggac 
gttgataag gaaattgtc ggtgttagg gagagtctt 
aagggggct cagggcggt ttgagggag ttgtttcag 
tcaagggct agtcgcaaa gggttggtt ataaccgtc 
ggtttgatg gttttgcag caaatggct gggatcaac 
gctgtgaat tcgtacttg cagacgatt ttcgatgca 
acaggaagc gggctttca ccggaaatc tcctcgatt 
attatagga acggttcag gtcttcacc acggtgtta 
acatcgtcg cttgtggat agatcgggg cggaggatt 
cttctttta ttttcaatg gtcgggtca ggtgtttct 
cttgtttcg cttgggtcg tatttctac ctcaaaagc 
aacagtttc caagtcgat acactctcc tggcttcca 
gtaatgagt ttagttgtt tttattatc tcgtttaat 
atcggcttg gggccgatt ccgtgggcc gtcatggcg 
gagatgttt ccgccaaat gttaaatca attgcttcg 
actttctcc agtattgtg tgttttatt gcggctttt 
accatcact ttgattttt ccgtcgttg gctgaggtg 
ctgggcatg gggcaggcc ttctgtaga atagttctc 
aagacagtg acgccaata agttcccca aaagcgctc 
accatggcc gaaccgtct ctccaagaa gtggtctac 
aagcccacc accgacacc ccaaccccc accgctcta 
aaaccctcc accgccccc acaaccagc gccttcctc 
agcttttcc gccgcaatc gccgacctg gccgccttc 
tccgccggc gtggccttc ggctggcct tcccccgtc 
ctcccgaag ctagccggc cacaacaac ccattgggc 
cgccccatc acccacacc caagcctcc tggatcgcc 
gggctggtc tgcttgggc gccatcctg ggcccgctg 
ctcgccggc cccgtcgcc gacaaactg ggccgcaaa 
aaggcccta atcctggcc gcgtgtccc atgactggg 
tccctgctc ctggcggcg tacgccacc acactccca 
tggttctac ctctcccgg ttcgccatg ggcgtgggc 
gccggcagc gtcttcacg gtcctgccc atctacttg 
gccgaaatc gcccaggac cacaaccgc ggcacgctg 
ggctgctcc atgggcgcg tttgtggct tctgggctg 
ttgttcgcc ttcgcggtg ggtcccttt ctggaagtg 
gggacgttt tgtctcgtt tgtaccctc ccattgctg 
gttttcctg gccgttttt tcggctttt gtcccagaa 
agtcccttt tttctggcc gcggctaac cggagccgt 
gacttagag caaagtttg atgaaattg aggaacagt 
gaaaatgtt ggggacgaa gttttggaa atcacgcag 
cgggttttt gaggagagg aaaatcaaa actggcctt 
ctggatttg ttcaaattc cgggcgttg aggcgcggt 
ttggttgta actcttgga attgtgatc ctgcagcaa 
tttgcggga attaacgcc gttttgtcg tacttgcag 
acgattttt gaggcttcc ggaagtggg caaagcccg 
gaaatggcc acaattatc atcggggtt gtgcaaatg 
gtcgcaact gtggtcacg tccctcctg gctgatcgt 
ctaggccgc agggttctg ctgctgact tcggcagtc 
gggtcaagt gttgcgctc ctggctcta gggctgtat 
ttctaccgc aagggccag cacctggaa gttggggcg 
atttcctgg ctgccggtg gccagtttg gtcgtgtac 
atggtggcg tttaatgtg ggcttgggg ccactgccg 
tgggctgtc atgggcgag ctttttccc tcaagtgtc 
aagtcagtg gcggcgggt tttacgtgt tttatctgc 
ttcgtggcc gcgtttgtt atcactctg ttgttcccg 
attttgtcg aatcttgtg gggatggcg aattcgttc 
tggtttttc gcagggatg tgtctttta ggggcgttt 
tttatctat tggatgctg ccggagacc aagggcaag 
agtgtgcaa gaaatacaa aaattgtta gggggggat 
tga
    mRNA sequence
    • >TC034160 RA
      gatttaata aatgtcccc atttaattt tcgcgtcat 
tatttaacc gaaaatgtc cagttttta cttgtacta 
taccgcgtg cgtggcgaa tttgattgc gttcactgt 
ggggcttgc ctacgggtt ggcgtcccc cctcctccc 
gagactcaa cggaagtgt ggaccccga taacaaccc 
actggaccc acccccaac cccttccga ggagtctct 
aatcgcgtc tttactatc acttggagc tattttcgg 
cccactttt aaccggtct ctttgttga taaaatcgg 
gcggaaaaa aacgctcct aatcgtggc tttgcccat 
aattgcgtc attcctgac catggcttt cgcccattc 
ggtggtact ttactacat cgcgcgttt ggtcatggg 
tgtgggggc ggggagcgt cttcacagt catgcccat 
gtacctggg ggagatctc cgaggaccg caaccgcgg 
cactttggg gtgtctcat gtcgacgtt tatcgcctc 
tggggtgct tttcgacta cgccgtggg gccgttttt 
gaccgttca gtgctactg tttggtgtg tttggcgcc 
tctgggggc gtttttggc gctgtttgg ggggtgggc 
ccccgaatc gccccaatt cctggcctt gaggggcga 
tgaggggcg cttgagggc gtgtttggc caagttgcg 
ggggtcgcg ggacgttga taaggaaat tgtcggtgt 
tagggagag tcttaaggg ggctcaggg cggtttgag 
ggagttgtt tcagtcaag ggctagtcg caaagggtt 
ggttataac cgtcggttt gatggtttt gcagcaaat 
ggctgggat caacgctgt gaattcgta cttgcagac 
gattttcga tgcaacagg aagcgggct ttcaccgga 
aatctcctc gattattat aggaacggt tcaggtctt 
caccacggt gttaacatc gtcgcttgt ggatagatc 
ggggcggag gattcttct tttattttc aatggtcgg 
gtcaggtgt ttctcttgt ttcgcttgg gtcgtattt 
ctacctcaa aagcaacag tttccaagt cgatacact 
ctcctggct tccagtaat gagtttagt tgtttttat 
tatctcgtt taatatcgg cttggggcc gattccgtg 
ggccgtcat ggcggagat gtttccgcc aaatgttaa 
atcaattgc ttcgacttt ctccagtat tgtgtgttt 
tattgcggc ttttaccat cactttgat ttttccgtc 
gttggctga ggtgctggg catggggca ggccttctg 
tagaatagt tctcaagac agtgacgcc aataagttc 
cccaaaagc gctcaccat ggccgaacc gtctctcca 
agaagtggt ctacaagcc caccaccga caccccaac 
ccccaccgc tctaaaacc ctccaccgc ccccacaac 
cagcgcctt cctcagctt ttccgccgc aatcgccga 
cctggccgc cttctccgc cggcgtggc cttcggctg 
gccttcccc cgtcctccc gaagctagc cggccacaa 
caacccatt gggccgccc catcaccca cacccaagc 
ctcctggat cgccgggct ggtctgctt gggcgccat 
cctgggccc gctgctcgc cggccccgt cgccgacaa 
actgggccg caaaaaggc cctaatcct ggccgcgtg 
tcccatgac tgggtccct gctcctggc ggcgtacgc 
caccacact cccatggtt ctacctctc ccggttcgc 
catgggcgt gggcgccgg cagcgtctt cacggtcct 
gcccatcta cttggccga aatcgccca ggaccacaa 
ccgcggcac gctgggctg ctccatggg cgcgtttgt 
ggcttctgg gctgttgtt cgccttcgc ggtgggtcc 
ctttctgga agtggggac gttttgtct cgtttgtac 
cctcccatt gctggtttt cctggccgt tttttcggc 
ttttgtccc agaaagtcc cttttttct ggccgcggc 
taaccggag ccgtgactt agagcaaag tttgatgaa 
attgaggaa cagtgaaaa tgttgggga cgaagtttt 
ggaaatcac gcagcgggt ttttgagga gaggaaaat 
caaaactgg ccttctgga tttgttcaa attccgggc 
gttgaggcg cggtttggt tgtaactct tggaattgt 
gatcctgca gcaatttgc gggaattaa cgccgtttt 
gtcgtactt gcagacgat ttttgaggc ttccggaag 
tgggcaaag cccggaaat ggccacaat tatcatcgg 
ggttgtgca aatggtcgc aactgtggt cacgtccct 
cctggctga tcgtctagg ccgcagggt tctgctgct 
gacttcggc agtcgggtc aagtgttgc gctcctggc 
tctagggct gtatttcta ccgcaaggg ccagcacct 
ggaagttgg ggcgatttc ctggctgcc ggtggccag 
tttggtcgt gtacatggt ggcgtttaa tgtgggctt 
ggggccact gccgtgggc tgtcatggg cgagctttt 
tccctcaag tgtcaagtc agtggcggc gggttttac 
gtgttttat ctgcttcgt ggccgcgtt tgttatcac 
tctgttgtt cccgatttt gtcgaatct tgtggggat 
ggcgaattc gttctggtt tttcgcagg gatgtgtct 
tttaggggc gttttttat ctattggat gctgccgga 
gaccaaggg caagagtgt gcaagaaat acaaaaatt 
gttaggggg ggattgatt gaataaaag cacttgcaa 
agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY