Status
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Gene information
General information
TC034160
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006667,TC006666
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12708528..12713590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034160
    iBeetle screen iB_01076

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034160 RA
      MSSFYLYYT ACVANLIAF TVGLAYGLA SPLLPRLNG 
      SVDPDNNPL DPPPTPSEE SLIASLLSL GAIFGPLLT 
      GLFVDKIGR KKTLLIVAL PIIASFLTM AFAHSVVLY 
      YIARLVMGV GAGSVFTVM PMYLGEISE DRNRGTLGC 
      LMSTFIASG VLFDYAVGP FLTVQCYCL VCLAPLGAF 
      LALFGGWAP ESPQFLALR GDEGRLRAC LAKLRGSRD 
      VDKEIVGVR ESLKGAQGG LRELFQSRA SRKGLVITV 
      GLMVLQQMA GINAVNSYL QTIFDATGS GLSPEISSI 
      IIGTVQVFT TVLTSSLVD RSGRRILLL FSMVGSGVS 
      LVSLGSYFY LKSNSFQVD TLSWLPVMS LVVFIISFN 
      IGLGPIPWA VMAEMFPPN VKSIASTFS SIVCFIAAF 
      TITLIFPSL AEVLGMGQA FCRIVLKTV TPISSPKAL 
      TMAEPSLQE VVYKPTTDT PTPTALKPS TAPTTSAFL 
      SFSAAIADL AAFSAGVAF GWPSPVLPK LAGHNNPLG 
      RPITHTQAS WIAGLVCLG AILGPLLAG PVADKLGRK 
      KALILAACP MTGSLLLAA YATTLPWFY LSRFAMGVG 
      AGSVFTVLP IYLAEIAQD HNRGTLGCS MGAFVASGL 
      LFAFAVGPF LEVGTFCLV CTLPLLVFL AVFSAFVPE 
      SPFFLAAAN RSRDLEQSL MKLRNSENV GDEVLEITQ 
      RVFEERKIK TGLLDLFKF RALRRGLVV TLGIVILQQ 
      FAGINAVLS YLQTIFEAS GSGQSPEMA TIIIGVVQM 
      VATVVTSLL ADRLGRRVL LLTSAVGSS VALLALGLY 
      FYRKGQHLE VGAISWLPV ASLVVYMVA FNVGLGPLP 
      WAVMGELFP SSVKSVAAG FTCFICFVA AFVITLLFP 
      ILSNLVGMA NSFWFFAGM CLLGAFFIY WMLPETKGK 
      SVQEIQKLL GGD
    CDS sequence
    • >TC034160 RA
      atgtccagt ttttacttg tactatacc gcgtgcgtg 
      gcgaatttg attgcgttc actgtgggg cttgcctac 
      gggttggcg tcccccctc ctcccgaga ctcaacgga 
      agtgtggac cccgataac aacccactg gacccaccc 
      ccaacccct tccgaggag tctctaatc gcgtcttta 
      ctatcactt ggagctatt ttcggccca cttttaacc 
      ggtctcttt gttgataaa atcgggcgg aaaaaaacg 
      ctcctaatc gtggctttg cccataatt gcgtcattc 
      ctgaccatg gctttcgcc cattcggtg gtactttac 
      tacatcgcg cgtttggtc atgggtgtg ggggcgggg 
      agcgtcttc acagtcatg cccatgtac ctgggggag 
      atctccgag gaccgcaac cgcggcact ttggggtgt 
      ctcatgtcg acgtttatc gcctctggg gtgcttttc 
      gactacgcc gtggggccg tttttgacc gttcagtgc 
      tactgtttg gtgtgtttg gcgcctctg ggggcgttt 
      ttggcgctg tttgggggg tgggccccc gaatcgccc 
      caattcctg gccttgagg ggcgatgag gggcgcttg 
      agggcgtgt ttggccaag ttgcggggg tcgcgggac 
      gttgataag gaaattgtc ggtgttagg gagagtctt 
      aagggggct cagggcggt ttgagggag ttgtttcag 
      tcaagggct agtcgcaaa gggttggtt ataaccgtc 
      ggtttgatg gttttgcag caaatggct gggatcaac 
      gctgtgaat tcgtacttg cagacgatt ttcgatgca 
      acaggaagc gggctttca ccggaaatc tcctcgatt 
      attatagga acggttcag gtcttcacc acggtgtta 
      acatcgtcg cttgtggat agatcgggg cggaggatt 
      cttctttta ttttcaatg gtcgggtca ggtgtttct 
      cttgtttcg cttgggtcg tatttctac ctcaaaagc 
      aacagtttc caagtcgat acactctcc tggcttcca 
      gtaatgagt ttagttgtt tttattatc tcgtttaat 
      atcggcttg gggccgatt ccgtgggcc gtcatggcg 
      gagatgttt ccgccaaat gttaaatca attgcttcg 
      actttctcc agtattgtg tgttttatt gcggctttt 
      accatcact ttgattttt ccgtcgttg gctgaggtg 
      ctgggcatg gggcaggcc ttctgtaga atagttctc 
      aagacagtg acgccaata agttcccca aaagcgctc 
      accatggcc gaaccgtct ctccaagaa gtggtctac 
      aagcccacc accgacacc ccaaccccc accgctcta 
      aaaccctcc accgccccc acaaccagc gccttcctc 
      agcttttcc gccgcaatc gccgacctg gccgccttc 
      tccgccggc gtggccttc ggctggcct tcccccgtc 
      ctcccgaag ctagccggc cacaacaac ccattgggc 
      cgccccatc acccacacc caagcctcc tggatcgcc 
      gggctggtc tgcttgggc gccatcctg ggcccgctg 
      ctcgccggc cccgtcgcc gacaaactg ggccgcaaa 
      aaggcccta atcctggcc gcgtgtccc atgactggg 
      tccctgctc ctggcggcg tacgccacc acactccca 
      tggttctac ctctcccgg ttcgccatg ggcgtgggc 
      gccggcagc gtcttcacg gtcctgccc atctacttg 
      gccgaaatc gcccaggac cacaaccgc ggcacgctg 
      ggctgctcc atgggcgcg tttgtggct tctgggctg 
      ttgttcgcc ttcgcggtg ggtcccttt ctggaagtg 
      gggacgttt tgtctcgtt tgtaccctc ccattgctg 
      gttttcctg gccgttttt tcggctttt gtcccagaa 
      agtcccttt tttctggcc gcggctaac cggagccgt 
      gacttagag caaagtttg atgaaattg aggaacagt 
      gaaaatgtt ggggacgaa gttttggaa atcacgcag 
      cgggttttt gaggagagg aaaatcaaa actggcctt 
      ctggatttg ttcaaattc cgggcgttg aggcgcggt 
      ttggttgta actcttgga attgtgatc ctgcagcaa 
      tttgcggga attaacgcc gttttgtcg tacttgcag 
      acgattttt gaggcttcc ggaagtggg caaagcccg 
      gaaatggcc acaattatc atcggggtt gtgcaaatg 
      gtcgcaact gtggtcacg tccctcctg gctgatcgt 
      ctaggccgc agggttctg ctgctgact tcggcagtc 
      gggtcaagt gttgcgctc ctggctcta gggctgtat 
      ttctaccgc aagggccag cacctggaa gttggggcg 
      atttcctgg ctgccggtg gccagtttg gtcgtgtac 
      atggtggcg tttaatgtg ggcttgggg ccactgccg 
      tgggctgtc atgggcgag ctttttccc tcaagtgtc 
      aagtcagtg gcggcgggt tttacgtgt tttatctgc 
      ttcgtggcc gcgtttgtt atcactctg ttgttcccg 
      attttgtcg aatcttgtg gggatggcg aattcgttc 
      tggtttttc gcagggatg tgtctttta ggggcgttt 
      tttatctat tggatgctg ccggagacc aagggcaag 
      agtgtgcaa gaaatacaa aaattgtta gggggggat 
      tga
    mRNA sequence
    • >TC034160 RA
      gatttaata aatgtcccc atttaattt tcgcgtcat 
      tatttaacc gaaaatgtc cagttttta cttgtacta 
      taccgcgtg cgtggcgaa tttgattgc gttcactgt 
      ggggcttgc ctacgggtt ggcgtcccc cctcctccc 
      gagactcaa cggaagtgt ggaccccga taacaaccc 
      actggaccc acccccaac cccttccga ggagtctct 
      aatcgcgtc tttactatc acttggagc tattttcgg 
      cccactttt aaccggtct ctttgttga taaaatcgg 
      gcggaaaaa aacgctcct aatcgtggc tttgcccat 
      aattgcgtc attcctgac catggcttt cgcccattc 
      ggtggtact ttactacat cgcgcgttt ggtcatggg 
      tgtgggggc ggggagcgt cttcacagt catgcccat 
      gtacctggg ggagatctc cgaggaccg caaccgcgg 
      cactttggg gtgtctcat gtcgacgtt tatcgcctc 
      tggggtgct tttcgacta cgccgtggg gccgttttt 
      gaccgttca gtgctactg tttggtgtg tttggcgcc 
      tctgggggc gtttttggc gctgtttgg ggggtgggc 
      ccccgaatc gccccaatt cctggcctt gaggggcga 
      tgaggggcg cttgagggc gtgtttggc caagttgcg 
      ggggtcgcg ggacgttga taaggaaat tgtcggtgt 
      tagggagag tcttaaggg ggctcaggg cggtttgag 
      ggagttgtt tcagtcaag ggctagtcg caaagggtt 
      ggttataac cgtcggttt gatggtttt gcagcaaat 
      ggctgggat caacgctgt gaattcgta cttgcagac 
      gattttcga tgcaacagg aagcgggct ttcaccgga 
      aatctcctc gattattat aggaacggt tcaggtctt 
      caccacggt gttaacatc gtcgcttgt ggatagatc 
      ggggcggag gattcttct tttattttc aatggtcgg 
      gtcaggtgt ttctcttgt ttcgcttgg gtcgtattt 
      ctacctcaa aagcaacag tttccaagt cgatacact 
      ctcctggct tccagtaat gagtttagt tgtttttat 
      tatctcgtt taatatcgg cttggggcc gattccgtg 
      ggccgtcat ggcggagat gtttccgcc aaatgttaa 
      atcaattgc ttcgacttt ctccagtat tgtgtgttt 
      tattgcggc ttttaccat cactttgat ttttccgtc 
      gttggctga ggtgctggg catggggca ggccttctg 
      tagaatagt tctcaagac agtgacgcc aataagttc 
      cccaaaagc gctcaccat ggccgaacc gtctctcca 
      agaagtggt ctacaagcc caccaccga caccccaac 
      ccccaccgc tctaaaacc ctccaccgc ccccacaac 
      cagcgcctt cctcagctt ttccgccgc aatcgccga 
      cctggccgc cttctccgc cggcgtggc cttcggctg 
      gccttcccc cgtcctccc gaagctagc cggccacaa 
      caacccatt gggccgccc catcaccca cacccaagc 
      ctcctggat cgccgggct ggtctgctt gggcgccat 
      cctgggccc gctgctcgc cggccccgt cgccgacaa 
      actgggccg caaaaaggc cctaatcct ggccgcgtg 
      tcccatgac tgggtccct gctcctggc ggcgtacgc 
      caccacact cccatggtt ctacctctc ccggttcgc 
      catgggcgt gggcgccgg cagcgtctt cacggtcct 
      gcccatcta cttggccga aatcgccca ggaccacaa 
      ccgcggcac gctgggctg ctccatggg cgcgtttgt 
      ggcttctgg gctgttgtt cgccttcgc ggtgggtcc 
      ctttctgga agtggggac gttttgtct cgtttgtac 
      cctcccatt gctggtttt cctggccgt tttttcggc 
      ttttgtccc agaaagtcc cttttttct ggccgcggc 
      taaccggag ccgtgactt agagcaaag tttgatgaa 
      attgaggaa cagtgaaaa tgttgggga cgaagtttt 
      ggaaatcac gcagcgggt ttttgagga gaggaaaat 
      caaaactgg ccttctgga tttgttcaa attccgggc 
      gttgaggcg cggtttggt tgtaactct tggaattgt 
      gatcctgca gcaatttgc gggaattaa cgccgtttt 
      gtcgtactt gcagacgat ttttgaggc ttccggaag 
      tgggcaaag cccggaaat ggccacaat tatcatcgg 
      ggttgtgca aatggtcgc aactgtggt cacgtccct 
      cctggctga tcgtctagg ccgcagggt tctgctgct 
      gacttcggc agtcgggtc aagtgttgc gctcctggc 
      tctagggct gtatttcta ccgcaaggg ccagcacct 
      ggaagttgg ggcgatttc ctggctgcc ggtggccag 
      tttggtcgt gtacatggt ggcgtttaa tgtgggctt 
      ggggccact gccgtgggc tgtcatggg cgagctttt 
      tccctcaag tgtcaagtc agtggcggc gggttttac 
      gtgttttat ctgcttcgt ggccgcgtt tgttatcac 
      tctgttgtt cccgatttt gtcgaatct tgtggggat 
      ggcgaattc gttctggtt tttcgcagg gatgtgtct 
      tttaggggc gttttttat ctattggat gctgccgga 
      gaccaaggg caagagtgt gcaagaaat acaaaaatt 
      gttaggggg ggattgatt gaataaaag cacttgcaa 
      agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY