Status
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Gene information
General information
TC034155
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006659,TC006658,TC006657
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12637351..12647112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034155
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01074

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034155 RA
      MPKIISLII YLAICAKTK GLNDKLMHP HPQTTPKIP 
      YKSDENTAK FWNEQAQST LKAHVSETI NTNVAKNVI 
      LFMGDGMSV PTLAATRVY MGGENFELS FDKFPHTAI 
      SKTYCVNYQ VPDSACTAT AYLGGVKGN LGTVGVTAH 
      VPWDDCQAM TNTSNNVPS IALWAQKQG KSTGFVTTA 
      RVTHASPAG VFGHSAQRE WETDTDILN DGKDPKICT 
      DLATQLVYH DGKNLNVIM GGGRYAFLP NDTKGEEGD 
      YGYRSDGVN LIEGWKKLR KGKSKYIWN REQLLGLDP 
      QDIDYVLGI FESDHCPFH LERDPAMDP TLTEMTESA 
      IKILSKNPD GFFLFVEGA RIDMGHHQA MAHLALEET 
      AEFSRAVER AVSLTSDHD TLIVVTSDH AHTMSMGGY 
      PFRGNDILG VVGDQAADG LAFTTLNYA NGPGFRKAE 
      DGARHDPSK DDLGDIEYR YPAEVPLDS ETHGGDDVI 
      IFARGPWAH LYRGVVEQN VIPHFMAYA SCIGNGEEN 
      ACKSKATLH GFATVLLFE RGGTLEGTV KNTAVTLGI 
      NTKSLCKPD SYNKKMRVE GTIFLALVA ATLPFLVSG 
      DDWMHPHLP KNPSKRSLE NNEENTAAY WTRNGLQAV 
      RERIERKRN ENMAKNVIL FLGDGMSIP TISAARVYL 
      GGEEKSLTF DKFPYTGLS KTYCVDQQV ADSACSATA 
      YLCGVKANY GTIGVTGDV KRDDCSSML NSTNHVHSI 
      AHHFQNSGK MTGVVTTAR VTHASPAGT YAHTAERDW 
      ESDNDVISA NHDPVTCRD IAWQLVHGD TGRGLNVIL 
      GGGRSNFIP QGEKDEDGF EGHRLDKVN LIEQWLVQK 
      KEMGYNAKY VWNREGLLK TDYNQTDYL LGLFNSDHV 
      TFNLKRDPN REPSLAEMT QAAIKLLDK SQNGYFLFV 
      EGARIDMGH HNAQARMAL DETIEFSKA VQAAVDLTD 
      ESETLIVVT SDHAHTMSY SGYAHRGND VLGIAGNAD 
      DTLPYTILN YANGPGYKA SQGQRYDVS NDDMSNAEY 
      RYPGISPLD AETHGGDDV AIFAQGPWA HLFTGVMEE 
      NVIPYLLAY ASCSIAMSF ITLILFLAF AQTWASSMH 
      PRLPLPTKH SPNPDERTS SFWIQNAQK TVSEKIKRE 
      LNQNVAKNV ILFLGDGMS IPTVSAARV YQSGEETEL 
      AFDKFPYTG LAKTYAVDR QVADSANTA TAYLTGIKA 
      NYGTIGVGP EVNRSDCQG MTDETNHAL SIAYYSQLK 
      NKRTGLVTT ARVTHASPA GVYAHTSDR NWESDTDVI 
      NDGQNPQIC SDIATQLLF SETGKKLNV VMGGGRKKL 
      LPKEMTDEE GHKGERSDG ANLIQKWQE QRINVKYAW 
      NRSQLIDSD VTFDNVLGL FAHDHISYN LERDNISDP 
      SLAEMTAAA IKTLNRGSE GYFLFVEGA RIDMAHHET 
      ASRIALDET IEFSKAIQV AVDLTDESD TLIVVTSDH 
      AHTMSYSGY AERGHDILE FGGDGSDGK VYTTLNYAN 
      GPGYKKPNS DGSRHDIAG DNYGDKHYA FPAVAPLYA 
      ETHGGDDVA VFARGPWAH LFSGVLEQN VIPHLMAYA 
      SCVGEGLTA CDQIN
    CDS sequence
    • >TC034155 RA
      atgcccaaa ataatttca ttaattatt tatctcgcc 
      atctgtgca aaaacaaaa gggttgaat gacaaactc 
      atgcacccc cacccccaa acaacccct aaaattcca 
      tacaaaagt gacgaaaac acggcgaaa ttttggaac 
      gaacaagcg caaagcacc ctcaaagcc cacgtttcg 
      gaaacaatt aacacaaat gttgcaaaa aacgtgatt 
      ttgtttatg ggggatggc atgtcagtg ccaaccctt 
      gccgcaacc agggtttac atggggggc gaaaatttc 
      gagctgtct ttcgacaaa ttcccccac actgccatt 
      tctaagaca tactgtgtc aattaccaa gtgcccgat 
      tcggcatgc accgccact gcatacctg ggtggggtc 
      aagggtaac ttgggcaca gttggggta acggcccat 
      gtcccatgg gacgattgc caggccatg accaacact 
      tccaacaac gtcccatcc atagccctt tgggcccaa 
      aaacagggt aaaagtacg ggttttgtg accacggcc 
      agagtcact catgcctcc ccagctggg gttttcggg 
      cactcggcc caacgggag tgggagacg gacaccgat 
      atactaaac gatggtaaa gaccctaaa atttgtacg 
      gacttggcc acccaactg gtgtaccat gatggtaaa 
      aacttgaac gtgatcatg ggcgggggc cggtacgcc 
      ttcctcccg aatgacact aagggggag gagggcgat 
      tatgggtac aggtcagat ggggtgaat ttgatcgaa 
      gggtggaag aaattgagg aagggaaag tcgaaatat 
      atctggaat cgggaacaa ctcctgggt cttgacccc 
      caggacatc gactatgtt ttagggatt tttgagagt 
      gaccattgc cccttccac ctggagcgg gacccagct 
      atggacccg acgctcacc gaaatgacc gaaagcgcc 
      atcaaaatc ctgtcgaag aaccctgat gggtttttc 
      ctgtttgtg gaaggcgcg aggatcgat atgggtcac 
      caccaggcc atggcccac ctggccctg gaggaaacc 
      gcagagttt tcccgagcc gttgaacgg gccgtgagt 
      ttgacctca gatcacgac accctgatt gtggtaacg 
      tccgaccat gcccatacc atgtccatg ggcggttac 
      ccgttccgt ggtaacgat attttgggt gtcgtgggc 
      gatcaagcc gccgatggg ttagccttc actacgctt 
      aattacgcc aatgggcct gggtttagg aaggcggag 
      gatggggca aggcatgac ccatctaag gatgatttg 
      ggtgatatt gagtacaga tacccggct gaagttccc 
      ctagattcg gagacacat ggtggggat gatgttatt 
      atttttgca agagggcca tgggcacat ttgtatagg 
      ggagtagtg gaacaaaat gtgatacca cattttatg 
      gcatatgct tcttgtata ggcaatggg gaggaaaat 
      gcgtgtaaa agcaaggca actttgcat gggtttgcc 
      acagttttg ttatttgag cggggaggg acgttagag 
      gggacagtc aagaacaca gcggttact ctcggtata 
      aatacaaaa tcactctgt aaacccgac tcctataat 
      aagaaaatg cgtgtcgag ggtacaatt tttttggca 
      cttgttgcg gcaacattg ccatttctt gtctcaggg 
      gacgactgg atgcacccc cacttgccc aaaaacccc 
      agcaaaagg tccctggaa aacaacgaa gaaaacacc 
      gccgcctac tggacgcga aacgggctc caagcggtg 
      cgagaaaga atcgaaagg aagcgcaac gaaaacatg 
      gcaaaaaac gtgatcctc ttccttggt gacggaatg 
      tcaatcccc actatctcc gccgccagg gtgtacctc 
      gggggcgaa gaaaaaagc ctaacattt gacaaattc 
      ccctacact ggactgtca aaaacctac tgtgtcgat 
      caacaagtg gccgactcc gcctgttcg gccacagcc 
      tacttatgc ggcgtcaag gccaactac ggcacaatc 
      ggtgtgacc ggtgacgtc aaacgtgac gactgttct 
      tccatgtta aattcaacc aatcacgtc cattcaatc 
      gcccaccat ttccaaaac agtggaaaa atgacagga 
      gtagtgacc acggcacgt gtgacccac gcctcgccc 
      gccggcacg tacgcacac acggcggaa cgtgattgg 
      gagagcgac aatgacgtc atctcggcc aatcacgac 
      ccggtcacg tgccgcgac atcgcctgg caattggtc 
      cacggcgac acggggcgg ggcctcaac gtgatttta 
      ggaggggga aggtcgaat tttatccct caaggtgaa 
      aaggacgag gacggtttc gaaggacac agactcgac 
      aaagtcaac ttgatcgag caatggctc gtgcagaag 
      aaagagatg ggttataac gccaaatat gtgtggaac 
      agagaggga ctattaaag accgactat aaccaaacc 
      gactatctt ttgggtttg tttaattcg gatcacgtg 
      acgtttaat ttgaaacgt gacccgaat cgtgagccg 
      tcattggcg gaaatgacg caagccgcc attaaacta 
      ttagacaag tcgcaaaat ggctacttc ctgtttgtg 
      gaaggcgca aggatcgac atggggcac cataacgcc 
      caggctagg atggctctg gacgaaaca atcgaattt 
      tcaaaagcg gtccaagcc gccgttgac ttgaccgac 
      gaatcggag actttgatt gtcgtgacg tcagaccac 
      gcccacacg atgagctac agcgggtac gcacatcgc 
      ggaaatgac gttttaggc attgccgga aatgccgat 
      gacacttta ccttacacg attttgaat tatgctaat 
      gggccaggg tataaagcc agtcaaggt cagcggtat 
      gacgtcagc aatgacgac atgtcaaat gccgagtat 
      cgctatccg gggatttcc ccattggac gccgagacg 
      catggcggc gatgacgta gccattttt gcgcaaggg 
      ccatgggcc caccttttc accggtgtc atggaggag 
      aacgtcata ccctacctt ctcgcctat gcatcttgc 
      tcgatcgcc atgtccttc atcactcta atccttttc 
      ctagccttt gcccaaacc tgggcctcc tctatgcac 
      ccccgtctc cccctcccc accaaacac tcccccaac 
      cccgacgaa cgaacctcc agcttctgg atccaaaac 
      gcccaaaaa accgtcagc gaaaaaatc aaacgcgaa 
      ctcaaccaa aacgtagcc aaaaacgtg attcttttc 
      ctgggggac ggcatgtca ataccaacc gtgtcggca 
      gccagggtg taccaatca ggggaggaa accgaatta 
      gctttcgac aaattcccc tacactgga ctagccaag 
      acgtacgcg gtcgacaga caagtggcc gattcggcc 
      aacacggcc acggcctac ttgaccgga attaaggca 
      aactacgga acaatcgga gtgggacct gaagtgaac 
      cgaagcgac tgtcaagga atgacagat gaaacaaac 
      cacgccctg tccatagcc tactactcc caattaaaa 
      aacaaacgg acaggtcta gtgaccacg gctcgcgtc 
      acccatgcc tcgcctgct ggggtttat gcccacacg 
      agtgacaga aactgggaa agtgacact gacgtcatt 
      aatgacggt cagaacccg caaatatgt tccgacatt 
      gccactcaa ttgttattt agtgaaact ggtaaaaag 
      ttgaacgtt gttatgggc gggggcaga aaaaagctc 
      ctccctaaa gaaatgaca gatgaggaa ggccacaaa 
      ggtgaaagg tcagacggc gccaacttg atccaaaaa 
      tggcaagag cagcgcatt aacgtcaag tatgcctgg 
      aaccgcagc caactgatc gactctgac gtgaccttt 
      gacaacgtt ttgggcctt tttgcccac gaccacatc 
      agttataac cttgaacgt gacaatatc agcgacccc 
      tcattggcc gaaatgacc gcggcggcc attaaaacc 
      ctaaatcgg ggttccgaa ggctacttt ctcttcgta 
      gaaggcgcc cgtatcgac atggcacac cacgaaacc 
      gcatcgcgg atagccttg gacgaaaca atcgaattc 
      tccaaggcc atccaagtc gccgttgac ttgaccgac 
      gaatccgat acgctgatc gtcgtgacg tcagaccac 
      gcccacaca atgagttac agcggatat gccgaacgc 
      ggccatgac atactcgaa tttggcggc gatggtagt 
      gatggtaaa gtctacacg accttgaat tacgccaat 
      gggcctggg tataaaaag ccaaattcg gatgggtca 
      cgtcatgat atcgcggga gataattat ggtgataag 
      cattacgca tttccggct gttgcgccc ttatatgcc 
      gaaacacat ggcggtgat gacgttgca gtttttgca 
      agaggacca tgggcccac ctttttagc ggggtcttg 
      gaacaaaat gttatcccg catttgatg gcctatgcc 
      tcttgtgtg ggcgaagga ttgactgct tgtgatcaa 
      atcaattaa
    mRNA sequence
    • >TC034155 RA
      tatttaaag caacaatcg gcaaaaaac agccacaaa 
      gtcgccaaa aatgcccaa aataatttc attaattat 
      ttatctcgc catctgtgc aaaaacaaa agggttgaa 
      tgacaaact catgcaccc ccaccccca aacaacccc 
      taaaattcc atacaaaag tgacgaaaa cacggcgaa 
      attttggaa cgaacaagc gcaaagcac cctcaaagc 
      ccacgtttc ggaaacaat taacacaaa tgttgcaaa 
      aaacgtgat tttgtttat gggggatgg catgtcagt 
      gccaaccct tgccgcaac cagggttta catgggggg 
      cgaaaattt cgagctgtc tttcgacaa attccccca 
      cactgccat ttctaagac atactgtgt caattacca 
      agtgcccga ttcggcatg caccgccac tgcatacct 
      gggtggggt caagggtaa cttgggcac agttggggt 
      aacggccca tgtcccatg ggacgattg ccaggccat 
      gaccaacac ttccaacaa cgtcccatc catagccct 
      ttgggccca aaaacaggg taaaagtac gggttttgt 
      gaccacggc cagagtcac tcatgcctc cccagctgg 
      ggttttcgg gcactcggc ccaacggga gtgggagac 
      ggacaccga tatactaaa cgatggtaa agaccctaa 
      aatttgtac ggacttggc cacccaact ggtgtacca 
      tgatggtaa aaacttgaa cgtgatcat gggcggggg 
      ccggtacgc cttcctccc gaatgacac taaggggga 
      ggagggcga ttatgggta caggtcaga tggggtgaa 
      tttgatcga agggtggaa gaaattgag gaagggaaa 
      gtcgaaata tatctggaa tcgggaaca actcctggg 
      tcttgaccc ccaggacat cgactatgt tttagggat 
      ttttgagag tgaccattg ccccttcca cctggagcg 
      ggacccagc tatggaccc gacgctcac cgaaatgac 
      cgaaagcgc catcaaaat cctgtcgaa gaaccctga 
      tgggttttt cctgtttgt ggaaggcgc gaggatcga 
      tatgggtca ccaccaggc catggccca cctggccct 
      ggaggaaac cgcagagtt ttcccgagc cgttgaacg 
      ggccgtgag tttgacctc agatcacga caccctgat 
      tgtggtaac gtccgacca tgcccatac catgtccat 
      gggcggtta cccgttccg tggtaacga tattttggg 
      tgtcgtggg cgatcaagc cgccgatgg gttagcctt 
      cactacgct taattacgc caatgggcc tgggtttag 
      gaaggcgga ggatggggc aaggcatga cccatctaa 
      ggatgattt gggtgatat tgagtacag atacccggc 
      tgaagttcc cctagattc ggagacaca tggtgggga 
      tgatgttat tatttttgc aagagggcc atgggcaca 
      tttgtatag gggagtagt ggaacaaaa tgtgatacc 
      acattttat ggcatatgc ttcttgtat aggcaatgg 
      ggaggaaaa tgcgtgtaa aagcaaggc aactttgca 
      tgggtttgc cacagtttt gttatttga gcggggagg 
      gacgttaga ggggacagt caagaacac agcggttac 
      tctcggtat aaatacaaa atcactctg taaacccga 
      ctcctataa taagaaaat gcgtgtcga gggtacaat 
      ttttttggc acttgttgc ggcaacatt gccatttct 
      tgtctcagg ggacgactg gatgcaccc ccacttgcc 
      caaaaaccc cagcaaaag gtccctgga aaacaacga 
      agaaaacac cgccgccta ctggacgcg aaacgggct 
      ccaagcggt gcgagaaag aatcgaaag gaagcgcaa 
      cgaaaacat ggcaaaaaa cgtgatcct cttccttgg 
      tgacggaat gtcaatccc cactatctc cgccgccag 
      ggtgtacct cgggggcga agaaaaaag cctaacatt 
      tgacaaatt cccctacac tggactgtc aaaaaccta 
      ctgtgtcga tcaacaagt ggccgactc cgcctgttc 
      ggccacagc ctacttatg cggcgtcaa ggccaacta 
      cggcacaat cggtgtgac cggtgacgt caaacgtga 
      cgactgttc ttccatgtt aaattcaac caatcacgt 
      ccattcaat cgcccacca tttccaaaa cagtggaaa 
      aatgacagg agtagtgac cacggcacg tgtgaccca 
      cgcctcgcc cgccggcac gtacgcaca cacggcgga 
      acgtgattg ggagagcga caatgacgt catctcggc 
      caatcacga cccggtcac gtgccgcga catcgcctg 
      gcaattggt ccacggcga cacggggcg gggcctcaa 
      cgtgatttt aggaggggg aaggtcgaa ttttatccc 
      tcaaggtga aaaggacga ggacggttt cgaaggaca 
      cagactcga caaagtcaa cttgatcga gcaatggct 
      cgtgcagaa gaaagagat gggttataa cgccaaata 
      tgtgtggaa cagagaggg actattaaa gaccgacta 
      taaccaaac cgactatct tttgggttt gtttaattc 
      ggatcacgt gacgtttaa tttgaaacg tgacccgaa 
      tcgtgagcc gtcattggc ggaaatgac gcaagccgc 
      cattaaact attagacaa gtcgcaaaa tggctactt 
      cctgtttgt ggaaggcgc aaggatcga catggggca 
      ccataacgc ccaggctag gatggctct ggacgaaac 
      aatcgaatt ttcaaaagc ggtccaagc cgccgttga 
      cttgaccga cgaatcgga gactttgat tgtcgtgac 
      gtcagacca cgcccacac gatgagcta cagcgggta 
      cgcacatcg cggaaatga cgttttagg cattgccgg 
      aaatgccga tgacacttt accttacac gattttgaa 
      ttatgctaa tgggccagg gtataaagc cagtcaagg 
      tcagcggta tgacgtcag caatgacga catgtcaaa 
      tgccgagta tcgctatcc ggggatttc cccattgga 
      cgccgagac gcatggcgg cgatgacgt agccatttt 
      tgcgcaagg gccatgggc ccacctttt caccggtgt 
      catggagga gaacgtcat accctacct tctcgccta 
      tgcatcttg ctcgatcgc catgtcctt catcactct 
      aatcctttt cctagcctt tgcccaaac ctgggcctc 
      ctctatgca cccccgtct ccccctccc caccaaaca 
      ctcccccaa ccccgacga acgaacctc cagcttctg 
      gatccaaaa cgcccaaaa aaccgtcag cgaaaaaat 
      caaacgcga actcaacca aaacgtagc caaaaacgt 
      gattctttt cctggggga cggcatgtc aataccaac 
      cgtgtcggc agccagggt gtaccaatc aggggagga 
      aaccgaatt agctttcga caaattccc ctacactgg 
      actagccaa gacgtacgc ggtcgacag acaagtggc 
      cgattcggc caacacggc cacggccta cttgaccgg 
      aattaaggc aaactacgg aacaatcgg agtgggacc 
      tgaagtgaa ccgaagcga ctgtcaagg aatgacaga 
      tgaaacaaa ccacgccct gtccatagc ctactactc 
      ccaattaaa aaacaaacg gacaggtct agtgaccac 
      ggctcgcgt cacccatgc ctcgcctgc tggggttta 
      tgcccacac gagtgacag aaactggga aagtgacac 
      tgacgtcat taatgacgg tcagaaccc gcaaatatg 
      ttccgacat tgccactca attgttatt tagtgaaac 
      tggtaaaaa gttgaacgt tgttatggg cgggggcag 
      aaaaaagct cctccctaa agaaatgac agatgagga 
      aggccacaa aggtgaaag gtcagacgg cgccaactt 
      gatccaaaa atggcaaga gcagcgcat taacgtcaa 
      gtatgcctg gaaccgcag ccaactgat cgactctga 
      cgtgacctt tgacaacgt tttgggcct ttttgccca 
      cgaccacat cagttataa ccttgaacg tgacaatat 
      cagcgaccc ctcattggc cgaaatgac cgcggcggc 
      cattaaaac cctaaatcg gggttccga aggctactt 
      tctcttcgt agaaggcgc ccgtatcga catggcaca 
      ccacgaaac cgcatcgcg gatagcctt ggacgaaac 
      aatcgaatt ctccaaggc catccaagt cgccgttga 
      cttgaccga cgaatccga tacgctgat cgtcgtgac 
      gtcagacca cgcccacac aatgagtta cagcggata 
      tgccgaacg cggccatga catactcga atttggcgg 
      cgatggtag tgatggtaa agtctacac gaccttgaa 
      ttacgccaa tgggcctgg gtataaaaa gccaaattc 
      ggatgggtc acgtcatga tatcgcggg agataatta 
      tggtgataa gcattacgc atttccggc tgttgcgcc 
      cttatatgc cgaaacaca tggcggtga tgacgttgc 
      agtttttgc aagaggacc atgggccca cctttttag 
      cggggtctt ggaacaaaa tgttatccc gcatttgat 
      ggcctatgc ctcttgtgt gggcgaagg attgactgc 
      ttgtgatca aatcaatta aaaaaaaac gcgaaaaaa 
      tttgtaata aagtgaatt tgttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY