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Gene information
General information
TC006652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12585261..12590423
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006652
    iBeetle screen iB_01073

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006652 RA
      MHSLWLTGR LISRAIFNG ISNISTAAD PNLQKKPDP 
      QLVSAVCGF SKERGIQRL IKGQFGDDA WFTARHKSA 
      DVLGVADGV GGWRAYGID PGEFSLHLM RTCEHLVKL 
      GRFTPTNPS ELLARSYCE LLHHKKAIL GSSTACVVV 
      LNRDNNTLY TANIGDSGF MVVRKGRVI RKSEEQQHY 
      FNTPFQLSL PPPGYQADV LSDQPDSAI TDNFPVEDG 
      DVILVATDG VFDNLPQNL LVNELKKVQ GERCASRLQ 
      MVANSIAWM ARNLSFDET FFSPFAQSA FANGINTIG 
      GKPDDITVL LATVAI
    CDS sequence
    • >TC006652 RA
      atgcactcg ttgtggcta actggtaga cttatatcg 
      cgtgctatt ttcaatgga atcagcaat atctcaacg 
      gcggccgat cccaacctt cagaaaaaa ccagacccg 
      cagttggtg tcggccgtt tgtggcttc tcaaaggag 
      aggggaata cagagactg attaaggga caattcggg 
      gatgacgcc tggtttacg gcgagacac aagtcggcc 
      gatgttttg ggtgtggcc gacggcgtg gggggctgg 
      agggcctac ggcatagac ccgggggag ttctccctc 
      cacctaatg cggacctgt gaacacctc gtcaaactc 
      ggccgtttc accccaaca aacccctcg gaactccta 
      gcccggagc tactgcgaa ttattgcac cacaagaag 
      gccattttg ggtagcagt actgcgtgc gttgtggtg 
      ctaaaccgc gacaacaac accctctac accgcaaat 
      atcggagat agtggcttt atggttgtg cgaaagggg 
      cgcgtgata cgtaaaagt gaagaacag cagcattat 
      ttcaatact ccgtttcaa ttgtcgtta ccgccgcct 
      ggataccag gccgatgtc ttgtccgat caaccggac 
      tccgctatt acggataat tttccggtt gaggatgga 
      gatgttatt ctggtggcc accgacgga gttttcgat 
      aatttaccc cagaattta ctcgtcaat gaactgaaa 
      aaggttcaa ggcgagcgt tgcgctagc cgtctccaa 
      atggtggcg aattccatt gcctggatg gcgcgcaat 
      ttatcgttc gatgagacg ttcttttca ccatttgcc 
      caaagtgct tttgccaac ggtatcaac acgatcggt 
      ggcaaaccg gacgacatc accgttctg ctggcgact 
      gtcgcaata tga
    mRNA sequence
    • >TC006652 RA
      gttaatcaa accgaaatg atcaatgaa taagttgtg 
      acgtgtggt gcgtggcac aaataaatc gaatcgagt 
      gtgcttggc gaaaacaag tgccttcta taattaacg 
      gagcgcgtg attttgaaa ccgacgccc gtcacgtga 
      cattccgac gctcacgag atgtcgcta acgacacga 
      ttttagcaa tggcgtcag caatgtggt ggtcgtgag 
      cacagctga ctagaattt ccccgtaat gtgtcctag 
      taggcgatt ggtggtgat ttgaaacaa ctgcgtgtt 
      agtcaaggc agccgcgac gctatgttt gtttttgtg 
      atagagtca agtccgagt gaaattttc taaagttat 
      tatataatg cactcgttg tggctaact ggtagactt 
      atatcgcgt gctattttc aatggaatc agcaatatc 
      tcaacggcg gccgatccc aaccttcag aaaaaacca 
      gacccgcag ttggtgtcg gccgtttgt ggcttctca 
      aaggagagg ggaatacag agactgatt aagggacaa 
      ttcggggat gacgcctgg tttacggcg agacacaag 
      tcggccgat gttttgggt gtggccgac ggcgtgggg 
      ggctggagg gcctacggc atagacccg ggggagttc 
      tccctccac ctaatgcgg acctgtgaa cacctcgtc 
      aaactcggc cgtttcacc ccaacaaac ccctcggaa 
      ctcctagcc cggagctac tgcgaatta ttgcaccac 
      aagaaggcc attttgggt agcagtact gcgtgcgtt 
      gtggtgcta aaccgcgac aacaacacc ctctacacc 
      gcaaatatc ggagatagt ggctttatg gttgtgcga 
      aaggggcgc gtgatacgt aaaagtgaa gaacagcag 
      cattatttc aatactccg tttcaattg tcgttaccg 
      ccgcctgga taccaggcc gatgtcttg tccgatcaa 
      ccggactcc gctattacg gataatttt ccggttgag 
      gatggagat gttattctg gtggccacc gacggagtt 
      ttcgataat ttaccccag aatttactc gtcaatgaa 
      ctgaaaaag gttcaaggc gagcgttgc gctagccgt 
      ctccaaatg gtggcgaat tccattgcc tggatggcg 
      cgcaattta tcgttcgat gagacgttc ttttcacca 
      tttgcccaa agtgctttt gccaacggt atcaacacg 
      atcggtggc aaaccggac gacatcacc gttctgctg 
      gcgactgtc gcaatatga gaaatttag gagaaatat 
      tgaatcggt tcctctatc gttaatttt attttattt 
      acgaatgct tgtagtgct ttcatttgg gaaattcaa 
      gtgctatta aagacgttt taagtgatc atgaaacca 
      aatttcatt attggaacc taatattta ggtttcgcc 
      gtgtttcac tcttgtttt aagttagga cgtatactc 
      atcacttgt tttatttgt gcaagactc tgtgaattt 
      ttagtttta ataaatagt tgttacgag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY