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Gene information
General information
TC006631 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12141811..12148755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006631
    iBeetle screen iB_01072

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006631 RA
      MLTGWRLHV SKLFLIPQR YVLGVMGFL AIVNAYTMR 
      VTLSVAITE MVEPSKAST HYDPDSCPG SLANTSSVT 
      NSDKLYPWT STQQGLILS SFYWGYVIT HIPGGMIAE 
      RFGGKYSLG LGILCTAIF TFITPFVIY ASDGHWGWV 
      VFVRVIEGL GEGTTFPAL NVLLAQWVP LHERSKIGT 
      LVFAGSQIG TVAGNAISG ALISVTQDW ASVFYVFGA 
      LAILWVFLW MVLCYPDPE THPFISDEE KEYLRKHIA 
      HVTRKKRQV PWYSIMTSA PVLALIAAQ IGHDWGFFT 
      MVTDLPKYM KDVLKFNVA QNGVWSSVP YIFMWLVSM 
      TSGWVCDHL VAKRYLSVT LARKIFTTI ASMGPAIFI 
      IAASYSGCD RDLAVVMFT IAMSFMGSF YCGMKVNAL 
      DLAPNFAGT LMAIVNGIG AITGIIVPY LVGALTEDH 
      TLMQWRVVF WIAFGVFAV TNLIFVVFA SGEVQSWNE 
      DLTDQKEMD LRKEQSAEK A
    CDS sequence
    • >TC006631 RA
      atgttgaca ggatggcgg ctgcacgtc tcaaaacta 
      tttctcatc cctcaacgg tacgtcctg ggcgtgatg 
      ggtttcctg gccatcgtg aacgcctac accatgcgc 
      gtcaccctt tccgtggcc ataacagaa atggtcgaa 
      cccagcaaa gcatcaacc cattacgac cctgattcc 
      tgtcccggt tctctcgct aacacttca agcgttacg 
      aacagcgac aagttgtac ccatggaca agcacccag 
      cagggttta attctgagt tccttctac tgggggtac 
      gtaataacc catatcccg ggcggtatg atcgcggaa 
      cgtttcggg gggaagtac tcactaggt cttgggata 
      ctttgcact gccattttc actttcatc acccctttc 
      gttatctac gcctctgat gggcactgg ggttgggtt 
      gttttcgtg cgtgttatc gagggtttg ggtgaaggt 
      accaccttc ccggcactt aacgttttg ctagctcag 
      tgggtacca ctccatgag cgttcaaaa attggtact 
      ttggtgttt gccggttcg caaattggt accgttgcc 
      ggaaatgcc atcagtggt gctttgatt tccgtaacg 
      caagattgg gcgtcggtg ttttacgtt tttggggct 
      ttggccatt ctttgggtg ttcctttgg atggtactg 
      tgttaccct gacccagaa acgcaccca tttatcagc 
      gacgaggaa aaggagtac cttcgcaag cacatagcc 
      catgtgacg cggaaaaaa cgccaagta ccatggtac 
      tccatcatg acctctgcc ccagttctg gccctgatt 
      gcggcccag attgggcac gactggggt ttctttacc 
      atggttacg gacttgccc aagtacatg aaagacgtt 
      cttaagttc aacgtggcc cagaatggg gtctggtcg 
      tcggtacca tacattttc atgtggttg gtttccatg 
      acttcgggc tgggtgtgt gaccatttg gtggccaaa 
      cgctatctg agtgtgact cttgcccgg aaaatcttc 
      acaaccatt gcatccatg ggtccggca attttcata 
      attgcggct tcttattct gggtgcgat cgcgacttg 
      gccgtggtc atgttcacc attgccatg agtttcatg 
      ggttcgttc tattgtggt atgaaggtc aatgcgttg 
      gacttggca cccaatttt gctggtact cttatggcc 
      atcgtgaac ggaatcggg gccatcacc gggatcata 
      gtaccatat ctggtcggc gccttaacc gaagaccac 
      actttgatg caatggagg gtcgttttc tggatcgcg 
      ttcggcgtt tttgccgtc accaacttg attttcgtc 
      gtttttgca agcggggaa gtacagagc tggaacgag 
      gacctgacc gaccagaag gaaatggac ttgaggaag 
      gaacaaagt gccgaaaag gcgtga
    mRNA sequence
    • >TC006631 RA
      ttgcaaaaa attaaataa cggtgattt attttggaa 
      cagaaggtc agcgtccat gtcaacaac ggaaatttt 
      caaatttcc gaccgtcgt acgtacgat tccatttgt 
      tccaagtga agtgatgga tcgtgatgt ttatttaca 
      acgtttaca aaattttgc tggaaaatc ggcgtccga 
      aagccacca tgacgtccg tcaacgaat ccagctatc 
      tcctcgaat cgcaaaaat ccaacgacc ggaactgta 
      agtgccact aataccaag ccaatgaag tgtgaaaaa 
      tcgagggac acatcgcat cgaaaatgc atctcgtgc 
      tccacgaaa ccttggcct gaatgaaat tttcgccaa 
      gttttccca ttcaaacga agcaataaa ctgtagaaa 
      aatatgtga caaacgcgt gtttccaat ccaatccaa 
      ataattaga aatggttaa aactgcgtt tttacggct 
      tttttatta cgctcatta tcattgaat gaattattg 
      aaaattgcg gttttcgaa caacaatcc actttaatt 
      aaacgctca caaaaacaa ataaatcgt tggcctggt 
      ttcccatac gtcaaattt tttgacaca agacgcacg 
      aaaattcgc cttatcacc tgttgatcg atcggtcga 
      aacaataga aaaatcgat aatgcgcct aagcccact 
      tgattcttc gtgatttat caaaatttc ccaaccgcg 
      gaatgtttc cgaataatt accgtcaag ttgccaggc 
      aacgcgcgc atttgacac attttggcg caaatcgtg 
      ttaccagga caattgaca gtttttggc gcgagattg 
      tcaaaaatc acggcacac tagaccgat gacagtttt 
      atcgtttta atcgggaaa caataggcg aaataattg 
      gtaatctgt gcgtaacat cttttgcga gagaaatcc 
      gttgacctt taagtcata atataaagc gagcacgtc 
      aagttaata ttttcagta ctgcggtca gctcgataa 
      ttaaaactt gggtcgtac tgaaaaaat aacaatcgc 
      ggttggcat tggcaatga cttaagcgt ttgcgaaat 
      ttcgaaaaa gtggcaaaa tgttgacag gatggcggc 
      tgcacgtct caaaactat ttctcatcc ctcaacggt 
      acgtcctgg gcgtgatgg gtttcctgg ccatcgtga 
      acgcctaca ccatgcgcg tcacccttt ccgtggcca 
      taacagaaa tggtcgaac ccagcaaag catcaaccc 
      attacgacc ctgattcct gtcccggtt ctctcgcta 
      acacttcaa gcgttacga acagcgaca agttgtacc 
      catggacaa gcacccagc agggtttaa ttctgagtt 
      ccttctact gggggtacg taataaccc atatcccgg 
      gcggtatga tcgcggaac gtttcgggg ggaagtact 
      cactaggtc ttgggatac tttgcactg ccattttca 
      ctttcatca cccctttcg ttatctacg cctctgatg 
      ggcactggg gttgggttg ttttcgtgc gtgttatcg 
      agggtttgg gtgaaggta ccaccttcc cggcactta 
      acgttttgc tagctcagt gggtaccac tccatgagc 
      gttcaaaaa ttggtactt tggtgtttg ccggttcgc 
      aaattggta ccgttgccg gaaatgcca tcagtggtg 
      ctttgattt ccgtaacgc aagattggg cgtcggtgt 
      tttacgttt ttggggctt tggccattc tttgggtgt 
      tcctttgga tggtactgt gttaccctg acccagaaa 
      cgcacccat ttatcagcg acgaggaaa aggagtacc 
      ttcgcaagc acatagccc atgtgacgc ggaaaaaac 
      gccaagtac catggtact ccatcatga cctctgccc 
      cagttctgg ccctgattg cggcccaga ttgggcacg 
      actggggtt tctttacca tggttacgg acttgccca 
      agtacatga aagacgttc ttaagttca acgtggccc 
      agaatgggg tctggtcgt cggtaccat acattttca 
      tgtggttgg tttccatga cttcgggct gggtgtgtg 
      accatttgg tggccaaac gctatctga gtgtgactc 
      ttgcccgga aaatcttca caaccattg catccatgg 
      gtccggcaa ttttcataa ttgcggctt cttattctg 
      ggtgcgatc gcgacttgg ccgtggtca tgttcacca 
      ttgccatga gtttcatgg gttcgttct attgtggta 
      tgaaggtca atgcgttgg acttggcac ccaattttg 
      ctggtactc ttatggcca tcgtgaacg gaatcgggg 
      ccatcaccg ggatcatag taccatatc tggtcggcg 
      ccttaaccg aagaccaca ctttgatgc aatggaggg 
      tcgttttct ggatcgcgt tcggcgttt ttgccgtca 
      ccaacttga ttttcgtcg tttttgcaa gcggggaag 
      tacagagct ggaacgagg acctgaccg accagaagg 
      aaatggact tgaggaagg aacaaagtg ccgaaaagg 
      cgtgatttt gtgatttat agactgcga attgtgatt 
      atttattgt aacttaagt aaaaaataa agtttttga 
      gagatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY