| General information | |||
| TC006625 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12075261..12077981 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006625 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01070 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
- >TC006625 RA
MFAIRNEDF ESDSDSNFE DEDELTRII PPEVEDTRE CMKARHILG ILGFLGFAN VYAMRVNLS VAIVAMVNN TAIPQPSTN SNIYDHCLV PNATNSTQP NTSDGEFAW DEAKQGIVL GSFFYGYVL TQVPGGRLA ELFGGKLVY GVGVLVTAV FTLLSPIAA RINFPLFIL VRVLEGMGE GVTFPSMHA MLARWIPPL ERSKFAAYV YAGANFGTV ISLPLSGWL CSLELDGGW PLCFYLFGF LGIIWFVFW CFLIYDSPS SHPRINPQE RAFILASIG PQDEDDKAS IPWLKMLTC VPLWAILIT QCGQSWAFY TQLTELPTY MSQILHFDI QANAFLSAI PYLTSWWCG ILISFVADW LLSRGYLSL STSYKVFNS LASIVPSLG ILGVAYVGC DRVAVQLLL AIPGALAGA VYAGNQMNH IALSPKYAG TMYGITNAA SNMCGFLAP YVIGVIIEG RETLGQWRL VFYLAAGIN IGANLFYIA FASAREQPW SRSQRLSTI N
- >TC006625 RA
atgtttgca atccgaaat gaggatttc gaaagcgac tccgacagc aactttgaa gacgaagac gaactaact cgtataatc ccacctgaa gtggaagac acccgagag tgcatgaaa gcgcggcat attctagga atcctcggt tttctgggc tttgccaat gtgtacgct atgcgtgtc aacctgtct gtggccatt gtggccatg gtgaataac acagcaatt ccgcaacct tccactaac agcaacatc tacgaccac tgtttggtc ccaaacgcc acaaactca acacagcca aatacatct gatggggaa tttgcgtgg gacgaagcc aagcaaggg attgttttg gggtcgttt ttctatggg tatgtgctt acccaggta ccggggggc cgtttggcc gagctgttt ggggggaag ttggtgtac ggtgtgggg gttttagtg acggcggtt ttcacgtta ttgagtccc attgcagcg aggattaat tttccactg tttatcctt gtgcgcgtt ctggagggc atgggcgaa ggggtcacg tttccctca atgcatgcc atgctggcg aggtggatt cctccactg gagaggagc aaatttgca gcatatgtt tatgcagga gccaacttt ggcaccgtc atatcgctc ccgctatcg ggttggctg tgctcgctg gaactggac ggcggttgg ccgctgtgt ttctactta ttcgggttc cttggcata atttggttc gtattttgg tgctttctc atctacgac tcgccttcg tcccatccg aggataaac ccgcaagag cgagctttc atcttagcc agcatcggc cctcaagac gaagacgac aaggcctcc atcccctgg ctcaaaatg ctaacttgc gtcccactt tgggcaatt ctgataacc cagtgtggc cagtcgtgg gctttttac acccagttg actgaactg cctacttac atgagccaa atcctccac tttgacatt caagctaac gcatttttg tccgcaatt ccgtatctt acaagttgg tggtgcggg attcttatc agtttcgtg gccgattgg ctgttgagt cgtggatat ttgtcgctc tctacttcg tacaaagtc tttaattct ttagcttca attgtccca tcgctgggt attttgggc gtggcgtat gttgggtgt gacagagtg gcggtgcag ctgcttttg gccattccg ggggctctt gcgggggcg gtttatgcc ggcaatcaa atgaatcac attgctctc agccccaag tatgcgggg accatgtac gggatcacg aatgcagcg tccaacatg tgcgggttt ctagccccc tatgtcatt ggtgtaatt attgaagga cgggaaacg ttgggtcag tggagactg gtgttttac ctagctgct ggtattaac attggggcg aatttgttt tacatagcg ttcgccagt gcaagggaa caaccgtgg tcaaggtca caaagactg agcaccatc aactga
- >TC006625 RA
gtagaacag ctgataaca ccagtgttt gggaagttg taaatacgg ttttaaaaa atatcgtaa cttatcatt gattttatt atttattta atgtcacat aatcagtat taatcgccg tctacgcat tacatatcg tgttattcc gttccgagt gactcattg ctggtgcga agagtcaag ctttgtcct agctctgtg tcagttatt atcatcgca aaaccatgt ttgcaatcc gaaatgagg atttcgaaa gcgactccg acagcaact ttgaagacg aagacgaac taactcgta taatcccac ctgaagtgg aagacaccc gagagtgca tgaaagcgc ggcatattc taggaatcc tcggttttc tgggctttg ccaatgtgt acgctatgc gtgtcaacc tgtctgtgg ccattgtgg ccatggtga ataacacag caattccgc aaccttcca ctaacagca acatctacg accactgtt tggtcccaa acgccacaa actcaacac agccaaata catctgatg gggaatttg cgtgggacg aagccaagc aagggattg ttttggggt cgtttttct atgggtatg tgcttaccc aggtaccgg ggggccgtt tggccgagc tgtttgggg ggaagttgg tgtacggtg tgggggttt tagtgacgg cggttttca cgttattga gtcccattg cagcgagga ttaattttc cactgttta tccttgtgc gcgttctgg agggcatgg gcgaagggg tcacgtttc cctcaatgc atgccatgc tggcgaggt ggattcctc cactggaga ggagcaaat ttgcagcat atgtttatg caggagcca actttggca ccgtcatat cgctcccgc tatcgggtt ggctgtgct cgctggaac tggacggcg gttggccgc tgtgtttct acttattcg ggttccttg gcataattt ggttcgtat tttggtgct ttctcatct acgactcgc cttcgtccc atccgagga taaacccgc aagagcgag ctttcatct tagccagca tcggccctc aagacgaag acgacaagg cctccatcc cctggctca aaatgctaa cttgcgtcc cactttggg caattctga taacccagt gtggccagt cgtgggctt tttacaccc agttgactg aactgccta cttacatga gccaaatcc tccactttg acattcaag ctaacgcat ttttgtccg caattccgt atcttacaa gttggtggt gcgggattc ttatcagtt tcgtggccg attggctgt tgagtcgtg gatatttgt cgctctcta cttcgtaca aagtcttta attctttag cttcaattg tcccatcgc tgggtattt tgggcgtgg cgtatgttg ggtgtgaca gagtggcgg tgcagctgc ttttggcca ttccggggg ctcttgcgg gggcggttt atgccggca atcaaatga atcacattg ctctcagcc ccaagtatg cggggacca tgtacggga tcacgaatg cagcgtcca acatgtgcg ggtttctag ccccctatg tcattggtg taattattg aaggacggg aaacgttgg gtcagtgga gactggtgt tttacctag ctgctggta ttaacattg gggcgaatt tgttttaca tagcgttcg ccagtgcaa gggaacaac cgtggtcaa ggtcacaaa gactgagca ccatcaact gacaggcgc tttatttgg cgatgcttg tcgatgcct agatcaaga ttttttaat accaatgaa aggttaaac ttctgctta agcggaagt ttacagtat cgttaaata gttaagtat tgaaaaatg tgattccct agaattatc caattcatt tactaggtg caatgaata ttacaaaaa tatttggtt ttgttcctt tcatattaa cccgattat agaatgtat acaggtttt acgggaaat caattatta aaaagtcaa attgtgaag actaatttt tgagtaatt tagaatcaa atattttat taaaatgtg taccttcta taatcatag cataattaa atgtgataa agccttatc tcacttttt atttattgt ttttttaag tttcttagc cagtgtaaa attggtgtt tacacctcc atgtatgaa caattttac gataatgtt tagatctat cgaacgggg taccgacta cgaaaggcc cactgaaag tttttaatt tccatatta atattgtat ataaatttt attgtaaaa tatattttt tctatttat
