Status
Please wait ...
Gene information
General information
TC006625 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 628139..630859
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006625
    iBeetle screen iB_01070
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006625 RA
      MFAIRNEDF ESDSDSNFE DEDELTRII PPEVEDTRE 
      CMKARHILG ILGFLGFAN VYAMRVNLS VAIVAMVNN 
      TAIPQPSTN SNIYDHCLV PNATNSTQP NTSDGEFAW 
      DEAKQGIVL GSFFYGYVL TQVPGGRLA ELFGGKLVY 
      GVGVLVTAV FTLLSPIAA RINFPLFIL VRVLEGMGE 
      GVTFPSMHA MLARWIPPL ERSKFAAYV YAGANFGTV 
      ISLPLSGWL CSLELDGGW PLCFYLFGF LGIIWFVFW 
      CFLIYDSPS SHPRINPQE RAFILASIG PQDEDDKAS 
      IPWLKMLTC VPLWAILIT QCGQSWAFY TQLTELPTY 
      MSQILHFDI QANAFLSAI PYLTSWWCG ILISFVADW 
      LLSRGYLSL STSYKVFNS LASIVPSLG ILGVAYVGC 
      DRVAVQLLL AIPGALAGA VYAGNQMNH IALSPKYAG 
      TMYGITNAA SNMCGFLAP YVIGVIIEG RETLGQWRL 
      VFYLAAGIN IGANLFYIA FASAREQPW SRSQRLSTI 
      N
    CDS sequence
    • >TC006625 RA
      atgtttgca atccgaaat gaggatttc gaaagcgac 
      tccgacagc aactttgaa gacgaagac gaactaact 
      cgtataatc ccacctgaa gtggaagac acccgagag 
      tgcatgaaa gcgcggcat attctagga atcctcggt 
      tttctgggc tttgccaat gtgtacgct atgcgtgtc 
      aacctgtct gtggccatt gtggccatg gtgaataac 
      acagcaatt ccgcaacct tccactaac agcaacatc 
      tacgaccac tgtttggtc ccaaacgcc acaaactca 
      acacagcca aatacatct gatggggaa tttgcgtgg 
      gacgaagcc aagcaaggg attgttttg gggtcgttt 
      ttctatggg tatgtgctt acccaggta ccggggggc 
      cgtttggcc gagctgttt ggggggaag ttggtgtac 
      ggtgtgggg gttttagtg acggcggtt ttcacgtta 
      ttgagtccc attgcagcg aggattaat tttccactg 
      tttatcctt gtgcgcgtt ctggagggc atgggcgaa 
      ggggtcacg tttccctca atgcatgcc atgctggcg 
      aggtggatt cctccactg gagaggagc aaatttgca 
      gcatatgtt tatgcagga gccaacttt ggcaccgtc 
      atatcgctc ccgctatcg ggttggctg tgctcgctg 
      gaactggac ggcggttgg ccgctgtgt ttctactta 
      ttcgggttc cttggcata atttggttc gtattttgg 
      tgctttctc atctacgac tcgccttcg tcccatccg 
      aggataaac ccgcaagag cgagctttc atcttagcc 
      agcatcggc cctcaagac gaagacgac aaggcctcc 
      atcccctgg ctcaaaatg ctaacttgc gtcccactt 
      tgggcaatt ctgataacc cagtgtggc cagtcgtgg 
      gctttttac acccagttg actgaactg cctacttac 
      atgagccaa atcctccac tttgacatt caagctaac 
      gcatttttg tccgcaatt ccgtatctt acaagttgg 
      tggtgcggg attcttatc agtttcgtg gccgattgg 
      ctgttgagt cgtggatat ttgtcgctc tctacttcg 
      tacaaagtc tttaattct ttagcttca attgtccca 
      tcgctgggt attttgggc gtggcgtat gttgggtgt 
      gacagagtg gcggtgcag ctgcttttg gccattccg 
      ggggctctt gcgggggcg gtttatgcc ggcaatcaa 
      atgaatcac attgctctc agccccaag tatgcgggg 
      accatgtac gggatcacg aatgcagcg tccaacatg 
      tgcgggttt ctagccccc tatgtcatt ggtgtaatt 
      attgaagga cgggaaacg ttgggtcag tggagactg 
      gtgttttac ctagctgct ggtattaac attggggcg 
      aatttgttt tacatagcg ttcgccagt gcaagggaa 
      caaccgtgg tcaaggtca caaagactg agcaccatc 
      aactga
    mRNA sequence
    • >TC006625 RA
      gtagaacag ctgataaca ccagtgttt gggaagttg 
      taaatacgg ttttaaaaa atatcgtaa cttatcatt 
      gattttatt atttattta atgtcacat aatcagtat 
      taatcgccg tctacgcat tacatatcg tgttattcc 
      gttccgagt gactcattg ctggtgcga agagtcaag 
      ctttgtcct agctctgtg tcagttatt atcatcgca 
      aaaccatgt ttgcaatcc gaaatgagg atttcgaaa 
      gcgactccg acagcaact ttgaagacg aagacgaac 
      taactcgta taatcccac ctgaagtgg aagacaccc 
      gagagtgca tgaaagcgc ggcatattc taggaatcc 
      tcggttttc tgggctttg ccaatgtgt acgctatgc 
      gtgtcaacc tgtctgtgg ccattgtgg ccatggtga 
      ataacacag caattccgc aaccttcca ctaacagca 
      acatctacg accactgtt tggtcccaa acgccacaa 
      actcaacac agccaaata catctgatg gggaatttg 
      cgtgggacg aagccaagc aagggattg ttttggggt 
      cgtttttct atgggtatg tgcttaccc aggtaccgg 
      ggggccgtt tggccgagc tgtttgggg ggaagttgg 
      tgtacggtg tgggggttt tagtgacgg cggttttca 
      cgttattga gtcccattg cagcgagga ttaattttc 
      cactgttta tccttgtgc gcgttctgg agggcatgg 
      gcgaagggg tcacgtttc cctcaatgc atgccatgc 
      tggcgaggt ggattcctc cactggaga ggagcaaat 
      ttgcagcat atgtttatg caggagcca actttggca 
      ccgtcatat cgctcccgc tatcgggtt ggctgtgct 
      cgctggaac tggacggcg gttggccgc tgtgtttct 
      acttattcg ggttccttg gcataattt ggttcgtat 
      tttggtgct ttctcatct acgactcgc cttcgtccc 
      atccgagga taaacccgc aagagcgag ctttcatct 
      tagccagca tcggccctc aagacgaag acgacaagg 
      cctccatcc cctggctca aaatgctaa cttgcgtcc 
      cactttggg caattctga taacccagt gtggccagt 
      cgtgggctt tttacaccc agttgactg aactgccta 
      cttacatga gccaaatcc tccactttg acattcaag 
      ctaacgcat ttttgtccg caattccgt atcttacaa 
      gttggtggt gcgggattc ttatcagtt tcgtggccg 
      attggctgt tgagtcgtg gatatttgt cgctctcta 
      cttcgtaca aagtcttta attctttag cttcaattg 
      tcccatcgc tgggtattt tgggcgtgg cgtatgttg 
      ggtgtgaca gagtggcgg tgcagctgc ttttggcca 
      ttccggggg ctcttgcgg gggcggttt atgccggca 
      atcaaatga atcacattg ctctcagcc ccaagtatg 
      cggggacca tgtacggga tcacgaatg cagcgtcca 
      acatgtgcg ggtttctag ccccctatg tcattggtg 
      taattattg aaggacggg aaacgttgg gtcagtgga 
      gactggtgt tttacctag ctgctggta ttaacattg 
      gggcgaatt tgttttaca tagcgttcg ccagtgcaa 
      gggaacaac cgtggtcaa ggtcacaaa gactgagca 
      ccatcaact gacaggcgc tttatttgg cgatgcttg 
      tcgatgcct agatcaaga ttttttaat accaatgaa 
      aggttaaac ttctgctta agcggaagt ttacagtat 
      cgttaaata gttaagtat tgaaaaatg tgattccct 
      agaattatc caattcatt tactaggtg caatgaata 
      ttacaaaaa tatttggtt ttgttcctt tcatattaa 
      cccgattat agaatgtat acaggtttt acgggaaat 
      caattatta aaaagtcaa attgtgaag actaatttt 
      tgagtaatt tagaatcaa atattttat taaaatgtg 
      taccttcta taatcatag cataattaa atgtgataa 
      agccttatc tcacttttt atttattgt ttttttaag 
      tttcttagc cagtgtaaa attggtgtt tacacctcc 
      atgtatgaa caattttac gataatgtt tagatctat 
      cgaacgggg taccgacta cgaaaggcc cactgaaag 
      tttttaatt tccatatta atattgtat ataaatttt 
      attgtaaaa tatattttt tctatttat