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Gene information
General information
TC006624 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 624339..628078
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006624
    iBeetle screen iB_01069
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006624 RA
      MSENGSPTL LNLRDFRIQ KKTLSHHSN NGAISSASN 
      GAVRRRIIA FSDSDSDCD SSGTNSQSA VTPKPNIVI 
      VPNSKNMKN PAVLEKENQ MKYLMSSFP NVDAMVIHD 
      VLSRHGFKV DEAASELAR CHAVEFNSQ GYAKWKEAN 
      TSNNPESLV KSTGKRKPS PESAPYNKR KKFKRRKSD 
      DDTDDSTGS NQDFKDRRV FDSDEDSDV EISDELTGD 
      KRKVFEFMQ TATESELQL MSFCSKKKA EAIIEARPF 
      TGWVDLVQK LSNNKNLNT DLLNAAQQV LMTRNNIRH 
      LMKKCTNLA QQMEKAVAA GAGVKAQPR ILSVSLRLT 
      NYQMVGLNW LAVLHAQRV NGILADEMG LGKTVQVIA 
      FLAYLKETF QAQNTHLVV VPSSTLDNW RSEFARWCP 
      QLRVFMYYG STEERRGFR VDFAKGILA DFDVILTTY 
      SLVGNSPEE RKMFRVTPM HYVIFDEAH MLKNMNTQR 
      YENLIRINA KHRILLTGT PLQNNLLEL MSLLIFVMP 
      KMFAEKTED LKSLFQKTS KSKQTDDTL PPFEREQIE 
      QAKRIMKPF VLRRLKCDV LQDLPKKID HVMKVPMAP 
      TQKEQYEAL VASYQNAAV EEESAYNGM SIMTDLRKL 
      SNHPLLLRY HYDEHQIRE MAKLLVRDP TYKDTVEEY 
      IVQDLLFMS DFEINKLTK IHRCLERYM LPDNLILTS 
      GKFLYLDKI LAELKQNGH RVLIFSQYV IMLNVMEDY 
      LKIRKHKYL RMDGSTPVN ERQDLVDEY MGDNSIFIF 
      LLSTRAGGL GINLTSADT VIIHDIDFN PYNDKQAED 
      RCHRMGQTR PVTVYRLVS QGTIEEGML EMNKEKLKL 
      ERQITTEET DNPDVKSVV RLLSSALGI DSSKANAFV 
      SPPKKKT
    CDS sequence
    • >TC006624 RA
      atgtcagag aacggcagc cccacgtta ttaaatttg 
      cgcgatttc cgaatacaa aaaaagacg ttaagtcac 
      cacagtaac aacggtgca attagctcc gcatccaat 
      ggggctgtg aggcgccga ataattgca ttttcagat 
      tctgattcc gactgtgat agttctgga acaaattct 
      caatctgcg gtgactcca aaaccaaat attgtcatt 
      gtccccaat tcgaaaaat atgaagaat ccggccgtt 
      ttggagaag gagaaccag atgaagtat ttaatgtcg 
      tcattcccg aatgttgat gctatggtt attcacgat 
      gtgctgtct cgtcatggg tttaaagta gacgaagcg 
      gcgtcggag ttggccagg tgtcacgct gtcgaattc 
      aattcgcaa ggttacgcc aagtggaag gaagcgaac 
      acttcaaac aatcccgaa agcctggtg aaaagcact 
      ggcaagagg aaaccctcc cctgagagc gcgccctac 
      aacaaacgg aaaaaattt aaacgtcgg aagtccgat 
      gacgacacg gacgacagc actggcagc aatcaggac 
      ttcaaagac cgtcgcgtg tttgacagt gacgaggat 
      tcggacgtc gaaattagc gatgaatta accggggac 
      aagaggaaa gtttttgaa ttcatgcaa acggcaact 
      gaaagcgag ctccaattg atgagtttc tgttcaaaa 
      aagaaggca gaggcgatc attgaagct agaccattt 
      actggctgg gtggattta gtacaaaaa ttgagtaac 
      aataaaaac ctaaacacg gatttacta aatgcggca 
      caacaagta cttatgact cggaataat attagacac 
      ttgatgaag aaatgtaca aacttggcc caacagatg 
      gagaaggct gttgctgca ggcgctggg gttaaagcc 
      caaccgagg attttatca gtttcgttg cgtttgact 
      aattatcaa atggttggg cttaattgg ttggcggtc 
      ctgcacgcg caacgggtg aacggtatt ttggccgat 
      gaaatgggt ctggggaaa actgtgcaa gttattgcc 
      ttcttggcc tatttgaag gagactttc caagcgcaa 
      aatactcat cttgtagtg gtgccttcg tcgacttta 
      gataattgg aggtccgaa ttcgcccgc tggtgcccc 
      caattacgc gtttttatg tactatggt agcacagag 
      gagagaagg ggcttccga gttgatttt gcaaagggg 
      attttggcc gatttcgat gtgatttta acaacttat 
      tcattggtc ggaaacagc ccagaggaa cgcaaaatg 
      ttccgcgta acaccgatg cattatgtg attttcgac 
      gaggcccac atgctaaaa aacatgaat actcagcgt 
      tacgaaaac ctgatcaga atcaatgcg aaacatcgc 
      attttgttg accggaacc ccgctccaa aacaatttg 
      cttgaactg atgtcgctg ttgattttc gtgatgcca 
      aaaatgttt gccgaaaaa accgaagat ttgaaaagt 
      ctgttccag aaaacttcc aagtcgaaa caaaccgac 
      gatacgttg ccgcccttt gagcgcgaa caaatcgaa 
      caggcgaaa cgaatcatg aaaccgttt gttttgaga 
      cgattaaag tgtgacgtt ttgcaagat cttcccaaa 
      aaaatcgac catgttatg aaggtgcca atggcaccc 
      actcagaaa gagcaatat gaggcttta gttgcctcg 
      taccaaaat gcagcggtt gaggaggaa agtgcctac 
      aatggcatg agcataatg accgatttg aggaaatta 
      agcaatcat ccgttgctc ttaagatac cactacgac 
      gagcatcaa attagagaa atggccaaa cttttagtc 
      agagacccg acgtacaag gacacggtg gaggaatac 
      atcgtccaa gatttgttg ttcatgtcc gactttgaa 
      atcaataag ttgaccaaa atccaccgg tgtttggag 
      cgctacatg cttcccgat aatctcatt ctaacatcg 
      ggtaaattt ttatacttg gataaaatc ctagcggag 
      ctcaagcaa aacgggcat cgagtgctc atatttagt 
      cagtatgtg atcatgttg aacgtaatg gaagactat 
      cttaaaatc cgcaagcac aagtatttg agaatggat 
      gggagcact ccagttaac gaaagacag gaccttgtt 
      gatgaatat atgggggat aacagtatt tttattttc 
      ctgctgtcg actcgagca gggggtctt ggtattaac 
      ctgacttcg gctgatact gtcattata cacgacatt 
      gattttaac ccgtataat gacaaacag gccgaggac 
      aggtgtcac aggatgggt cagacgcga cctgtgact 
      gtgtacaga ttagtttct cagggtact atagaggag 
      ggaatgctc gaaatgaat aaagaaaag ctgaaattg 
      gagagacag ataaccacc gaagaaaca gataatcct 
      gatgtcaag agtgtggtg agattgctg tcttcagcc 
      ttgggtatt gatagttca aaagccaac gcgttcgtg 
      tccccacca aaaaagaag acgtga
    mRNA sequence
    • >TC006624 RA
      atgaattga agtacgaaa agtgtgctt tggattaat 
      tttacgttt cattgtttt aagacgcta cacaaaaaa 
      aaccaattt tacttcaac tggtgttta tttacaata 
      aatgcgtta aataattta acgttaacg ttgggtaga 
      ggactttgt attttcggg aaatttatt atttattag 
      tttatgaga gttggtttt aaattactt gcgcgaatt 
      ttgcgcttt tgcctcatt gctgccacc tgtgagcgg 
      caataatcc cactcttct accatcgtc tgtcaaagg 
      tgtctggtg cctcaatga gaacgattt tgtagtttt 
      atacttaat attttatgt tatttataa aattgtaac 
      tggcgcaat gtcagagaa cggcagccc cacgttatt 
      aaatttgcg cgatttccg aatacaaaa aaagacgtt 
      aagtcacca cagtaacaa cggtgcaat tagctccgc 
      atccaatgg ggctgtgag gcgccgaat aattgcatt 
      ttcagattc tgattccga ctgtgatag ttctggaac 
      aaattctca atctgcggt gactccaaa accaaatat 
      tgtcattgt ccccaattc gaaaaatat gaagaatcc 
      ggccgtttt ggagaagga gaaccagat gaagtattt 
      aatgtcgtc attcccgaa tgttgatgc tatggttat 
      tcacgatgt gctgtctcg tcatgggtt taaagtaga 
      cgaagcggc gtcggagtt ggccaggtg tcacgctgt 
      cgaattcaa ttcgcaagg ttacgccaa gtggaagga 
      agcgaacac ttcaaacaa tcccgaaag cctggtgaa 
      aagcactgg caagaggaa accctcccc tgagagcgc 
      gccctacaa caaacggaa aaaatttaa acgtcggaa 
      gtccgatga cgacacgga cgacagcac tggcagcaa 
      tcaggactt caaagaccg tcgcgtgtt tgacagtga 
      cgaggattc ggacgtcga aattagcga tgaattaac 
      cggggacaa gaggaaagt ttttgaatt catgcaaac 
      ggcaactga aagcgagct ccaattgat gagtttctg 
      ttcaaaaaa gaaggcaga ggcgatcat tgaagctag 
      accatttac tggctgggt ggatttagt acaaaaatt 
      gagtaacaa taaaaacct aaacacgga tttactaaa 
      tgcggcaca acaagtact tatgactcg gaataatat 
      tagacactt gatgaagaa atgtacaaa cttggccca 
      acagatgga gaaggctgt tgctgcagg cgctggggt 
      taaagccca accgaggat tttatcagt ttcgttgcg 
      tttgactaa ttatcaaat ggttgggct taattggtt 
      ggcggtcct gcacgcgca acgggtgaa cggtatttt 
      ggccgatga aatgggtct ggggaaaac tgtgcaagt 
      tattgcctt cttggccta tttgaagga gactttcca 
      agcgcaaaa tactcatct tgtagtggt gccttcgtc 
      gactttaga taattggag gtccgaatt cgcccgctg 
      gtgccccca attacgcgt ttttatgta ctatggtag 
      cacagagga gagaagggg cttccgagt tgattttgc 
      aaaggggat tttggccga tttcgatgt gattttaac 
      aacttattc attggtcgg aaacagccc agaggaacg 
      caaaatgtt ccgcgtaac accgatgca ttatgtgat 
      tttcgacga ggcccacat gctaaaaaa catgaatac 
      tcagcgtta cgaaaacct gatcagaat caatgcgaa 
      acatcgcat tttgttgac cggaacccc gctccaaaa 
      caatttgct tgaactgat gtcgctgtt gattttcgt 
      gatgccaaa aatgtttgc cgaaaaaac cgaagattt 
      gaaaagtct gttccagaa aacttccaa gtcgaaaca 
      aaccgacga tacgttgcc gccctttga gcgcgaaca 
      aatcgaaca ggcgaaacg aatcatgaa accgtttgt 
      tttgagacg attaaagtg tgacgtttt gcaagatct 
      tcccaaaaa aatcgacca tgttatgaa ggtgccaat 
      ggcacccac tcagaaaga gcaatatga ggctttagt 
      tgcctcgta ccaaaatgc agcggttga ggaggaaag 
      tgcctacaa tggcatgag cataatgac cgatttgag 
      gaaattaag caatcatcc gttgctctt aagatacca 
      ctacgacga gcatcaaat tagagaaat ggccaaact 
      tttagtcag agacccgac gtacaagga cacggtgga 
      ggaatacat cgtccaaga tttgttgtt catgtccga 
      ctttgaaat caataagtt gaccaaaat ccaccggtg 
      tttggagcg ctacatgct tcccgataa tctcattct 
      aacatcggg taaattttt atacttgga taaaatcct 
      agcggagct caagcaaaa cgggcatcg agtgctcat 
      atttagtca gtatgtgat catgttgaa cgtaatgga 
      agactatct taaaatccg caagcacaa gtatttgag 
      aatggatgg gagcactcc agttaacga aagacagga 
      ccttgttga tgaatatat gggggataa cagtatttt 
      tattttcct gctgtcgac tcgagcagg gggtcttgg 
      tattaacct gacttcggc tgatactgt cattataca 
      cgacattga ttttaaccc gtataatga caaacaggc 
      cgaggacag gtgtcacag gatgggtca gacgcgacc 
      tgtgactgt gtacagatt agtttctca gggtactat 
      agaggaggg aatgctcga aatgaataa agaaaagct 
      gaaattgga gagacagat aaccaccga agaaacaga 
      taatcctga tgtcaagag tgtggtgag attgctgtc 
      ttcagcctt gggtattga tagttcaaa agccaacgc 
      gttcgtgtc cccaccaaa aaagaagac gtgaacgtg 
      ttatgtttt agttactgt tagttttcc cgtaactga 
      tctgcattt gttaagtta gtctctgtg ttaatcaac 
      aataaaata ttttcctta at