Status
Please wait ...
Gene information
General information
TC006622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12067811..12068918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006622
    iBeetle screen iB_01068

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006622 RA
      MLWADKYRP KELSSLSYH KQQALDLRN LTKDGDFPH 
      LLVYGPLGA GKKTRIMCL LRELYGPGV DRLKVETMN 
      FTTPSNKKL EISTVGSNY HIEVNPSDV GINDRVVIM 
      DVIKNVAQS QQLNANTQR EFKVILLTD VDNLTKDAQ 
      HALRRTMEK YIANCRIIL CTTSISRVI PAIRSRCLC 
      IRVPAPSID DITSILTNV AAKESVSLP PELAKRIAE 
      KSDRNLRRA LLMCEACKV QKQGLVASQ SVSEPDWQI 
      FIRNIASKI VKEQSIATL AKIRENLYE LIIFGIPSE 
      IIFNTLLEE MLKNCDLEL GRQIVEQAA LYEHRMAQG 
      NKEIFHLEA FVAKCMCLY QNMMQELMA CEF
    CDS sequence
    • >TC006622 RA
      atgttgtgg gccgataag taccgccct aaggagctc 
      tcgtccctc agctaccac aagcagcaa gccctcgat 
      ttgaggaac ctgaccaag gatggtgac tttccccat 
      ttgttggtg tatggcccc cttggggcc ggaaagaag 
      acccgcatt atgtgcctt ctgcgtgaa ctgtacggc 
      ccaggtgtg gaccgtttg aaggtcgag accatgaat 
      ttcaccaca ccgtcaaac aagaagctc gaaattagc 
      acagtcggg agcaattat cacatcgaa gtgaatccc 
      tcggacgtt ggtataaac gaccgtgtt gtgatcatg 
      gacgtgatc aagaacgtg gcccagagc cagcaactc 
      aacgcaaac acgcaacgc gaatttaaa gttatcctg 
      ctgaccgat gttgataat ttgaccaag gatgctcag 
      catgccctt cgcaggact atggagaaa tacatagcg 
      aactgtcgg attattttg tgcactacg tccatttcc 
      agagttatt ccggcgata aggtcccgg tgtttgtgt 
      attagagtc ccagcgcct agcattgac gacataact 
      tcgattcta acaaacgtg gcagcgaaa gaatcagtg 
      tcgttgcct ccagaactt gcaaaacga atcgctgag 
      aagtcggac cgcaaccta cgacgcgcc cttctcatg 
      tgtgaagct tgcaaagtc caaaaacaa ggactggta 
      gcaagtcaa agtgttagt gaacccgac tggcagatt 
      ttcatccgg aacattgcc agtaaaatt gtcaaggaa 
      caaagcatt gccactttg gccaaaatt agggagaat 
      ttgtacgag ttgatcatt tttggcatt ccgtccgaa 
      atcatcttc aacactctt ctcgaagaa atgttgaag 
      aattgtgac ctggagctc ggccgccaa atagtggaa 
      caggcggct ttgtacgaa caccgcatg gcgcagggg 
      aacaaggag atcttccac ttggaggct ttcgtggcc 
      aagtgcatg tgcttgtac caaaacatg atgcaagag 
      ctcatggct tgcgagttt taa
    mRNA sequence
    • >TC006622 RA
      ttggcgccc aaaaagttg aaaatgttg tgggccgat 
      aagtaccgc cctaaggag ctctcgtcc ctcagctac 
      cacaagcag caagccctc gatttgagg aacctgacc 
      aaggatggt gactttccc catttgttg gtgtatggc 
      ccccttggg gccggaaag aagacccgc attatgtgc 
      cttctgcgt gaactgtac ggcccaggt gtggaccgt 
      ttgaaggtc gagaccatg aatttcacc acaccgtca 
      aacaagaag ctcgaaatt agcacagtc gggagcaat 
      tatcacatc gaagtgaat ccctcggac gttggtata 
      aacgaccgt gttgtgatc atggacgtg atcaagaac 
      gtggcccag agccagcaa ctcaacgca aacacgcaa 
      cgcgaattt aaagttatc ctgctgacc gatgttgat 
      aatttgacc aaggatgct cagcatgcc cttcgcagg 
      actatggag aaatacata gcgaactgt cggattatt 
      ttgtgcact acgtccatt tccagagtt attccggcg 
      ataaggtcc cggtgtttg tgtattaga gtcccagcg 
      cctagcatt gacgacata acttcgatt ctaacaaac 
      gtggcagcg aaagaatca gtgtcgttg cctccagaa 
      cttgcaaaa cgaatcgct gagaagtcg gaccgcaac 
      ctacgacgc gcccttctc atgtgtgaa gcttgcaaa 
      gtccaaaaa caaggactg gtagcaagt caaagtgtt 
      agtgaaccc gactggcag attttcatc cggaacatt 
      gccagtaaa attgtcaag gaacaaagc attgccact 
      ttggccaaa attagggag aatttgtac gagttgatc 
      atttttggc attccgtcc gaaatcatc ttcaacact 
      cttctcgaa gaaatgttg aagaattgt gacctggag 
      ctcggccgc caaatagtg gaacaggcg gctttgtac 
      gaacaccgc atggcgcag gggaacaag gagatcttc 
      cacttggag gctttcgtg gccaagtgc atgtgcttg 
      taccaaaac atgatgcaa gagctcatg gcttgcgag 
      ttttaatta ataaagttg cttaattat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY