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Gene information
General information
TC006614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11984041..11990201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006614
    iBeetle screen iB_01067

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late rounded - Number of animals showing the phenotype: 3

    vitellogenic egg chamber segmentation/division irregular - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006614 RA
      MASLWAGSD VDLSVLHQC RIAERLEFR LACLHKQKI 
      PDSDTKCEG EIHKFRQCR RRSGTWPRT RSLNAPSPI 
      QQFGPCGRI MKNSAMVII SSDCDISVS VVNFSEMVA 
      NGNVVCLPF AEEINNNNN MKFQYRTIQ DPASQRLTW 
      YKPPLTVLV IKKVRDASV HLPFVQLVQ WLIEEKRML 
      VFVEAAVLE DPLLDQFTS FATIKDKLM TFRDGKDDL 
      TDKIDFIIC LGGDGTLLY ASHLFQQSV PPVMAFHLG 
      SLGFLTPFR FDNFQEQVN NVLEGNAAL TLRSRLRCI 
      IMRKGDDEK KTSKQPTNL LVLNEVVVD RGPSPYLSN 
      IDLFLDGKL ITSVQGDGL IVSTPTGST AYAVAAGAS 
      MIHPSVPAI MVTPICPHS LSFRPIVVP AGVELKISV 
      SRDSRNTSW VSFDGRNRQ ELLHGDSLR VTTSIYPVP 
      SICAQDQIS DWFDSLAEC LHWNVRKRQ KHLDELTDL 
      THSSSNDTL DSLEHAELI EKDS
    CDS sequence
    • >TC006614 RA
      atggcatcg ctatgggcc ggttcggat gtggatttg 
      tccgttttg caccagtgc aggatagcc gaaaggtta 
      gagttcaga ttggcctgt ttgcataaa cagaaaatc 
      cctgattct gatacgaaa tgcgagggg gaaattcac 
      aaattcagg cagtgcagg agacggtca ggcacttgg 
      ccgaggacg aggtctctg aatgcaccg tcgcccata 
      caacagttt ggaccatgt ggacgcatt atgaaaaat 
      tccgcaatg gtcattatt agctcagac tgtgatatt 
      agtgtgtca gtggtaaat ttttccgaa atggtcgcg 
      aacggaaac gtcgtttgt ctcccgttc gccgaagaa 
      atcaacaac aataacaac atgaaattt cagtatagg 
      accatccag gatccggcg agccaaagg ctgacgtgg 
      tacaaaccg ccacttact gtcctagtg ataaaaaaa 
      gtgagggat gcctcggtc catttgccg tttgtgcag 
      ctggtgcaa tggcttata gaggaaaaa cggatgctt 
      gtgttcgtg gaggcggcc gtcctggag gaccccctc 
      cttgaccag ttcacctcc tttgccacc atcaaagac 
      aagctgatg acgttccgc gacggcaag gacgatctc 
      accgacaaa atcgacttt attatctgt ctcggcggt 
      gacggcacc ctcctctac gccagccac cttttccag 
      caatctgtg ccccctgtc atggccttc cacctcggt 
      tccttgggg ttcctcacc cctttccgc ttcgacaac 
      ttccaagag caagtcaac aacgtgctc gaaggcaac 
      gccgcctta actctccga agtcgcttg cgatgtatc 
      atcatgcga aagggtgac gacgagaag aagacgtcc 
      aagcagccg acgaatctc ctggtgctc aacgaggtt 
      gtcgttgat cgaggaccc tcaccttac ctctcaaac 
      atcgatctt ttcctcgat ggaaaattg atcacttcg 
      gtgcaaggg gacgggctg atcgtgagc acgcccact 
      ggaagcacg gcctatgcc gtggcggct ggcgcgtcc 
      atgatccat ccgagcgtg ccggcgatc atggtcacg 
      cccatttgc ccccactcg ttgagtttc cggccaatt 
      gtcgtgccg gcgggggtg gagttaaag atttctgtc 
      tctcgtgac agtcgcaat acgtcatgg gtgtcgttt 
      gacggccgc aaccgccaa gagctgtta catggtgac 
      agtttgcgt gtgacaacg tcgatttat ccagtgccg 
      agcatttgc gcacaagac cagatttcc gattggttc 
      gattcgctg gctgagtgt ctgcactgg aacgtgagg 
      aaaagacag aaacatttg gacgagttg acggatttg 
      acgcactct agttcgaat gatacgttg gattcgttg 
      gagcacgcc gagttgatc gagaaggat agctag
    mRNA sequence
    • >TC006614 RA
      ttgtttaaa aatagaaaa ttttgggat ggcccatta 
      atttgaaat aaaatccgg gttgttgcc gtcacctgt 
      cattaaagc tgtcatttg tcaaatttt cccaaaaat 
      gacgtgtaa caacaaaaa cccgaaacc gtcgtgaaa 
      ttcctcgtt ggtgtgtga aagtgtgcc cttttcggt 
      cttccattc tcgctccta gtgcggtcc tgcgtctca 
      tatctgtca aaatgtcaa agtctttga cctaaccta 
      gtttcaata tttgcgaaa taaaggcgt attatggca 
      tcgctatgg gccggttcg gatgtggat ttgtccgtt 
      ttgcaccag tgcaggata gccgaaagg ttagagttc 
      agattggcc tgtttgcat aaacagaaa atccctgat 
      tctgatacg aaatgcgag ggggaaatt cacaaattc 
      aggcagtgc aggagacgg tcaggcact tggccgagg 
      acgaggtct ctgaatgca ccgtcgccc atacaacag 
      tttggacca tgtggacgc attatgaaa aattccgca 
      atggtcatt attagctca gactgtgat attagtgtg 
      tcagtggta aatttttcc gaaatggtc gcgaacgga 
      aacgtcgtt tgtctcccg ttcgccgaa gaaatcaac 
      aacaataac aacatgaaa tttcagtat aggaccatc 
      caggatccg gcgagccaa aggctgacg tggtacaaa 
      ccgccactt actgtccta gtgataaaa aaagtgagg 
      gatgcctcg gtccatttg ccgtttgtg cagctggtg 
      caatggctt atagaggaa aaacggatg cttgtgttc 
      gtggaggcg gccgtcctg gaggacccc ctccttgac 
      cagttcacc tcctttgcc accatcaaa gacaagctg 
      atgacgttc cgcgacggc aaggacgat ctcaccgac 
      aaaatcgac tttattatc tgtctcggc ggtgacggc 
      accctcctc tacgccagc caccttttc cagcaatct 
      gtgccccct gtcatggcc ttccacctc ggttccttg 
      gggttcctc acccctttc cgcttcgac aacttccaa 
      gagcaagtc aacaacgtg ctcgaaggc aacgccgcc 
      ttaactctc cgaagtcgc ttgcgatgt atcatcatg 
      cgaaagggt gacgacgag aagaagacg tccaagcag 
      ccgacgaat ctcctggtg ctcaacgag gttgtcgtt 
      gatcgagga ccctcacct tacctctca aacatcgat 
      cttttcctc gatggaaaa ttgatcact tcggtgcaa 
      ggggacggg ctgatcgtg agcacgccc actggaagc 
      acggcctat gccgtggcg gctggcgcg tccatgatc 
      catccgagc gtgccggcg atcatggtc acgcccatt 
      tgcccccac tcgttgagt ttccggcca attgtcgtg 
      ccggcgggg gtggagtta aagatttct gtctctcgt 
      gacagtcgc aatacgtca tgggtgtcg tttgacggc 
      cgcaaccgc caagagctg ttacatggt gacagtttg 
      cgtgtgaca acgtcgatt tatccagtg ccgagcatt 
      tgcgcacaa gaccagatt tccgattgg ttcgattcg 
      ctggctgag tgtctgcac tggaacgtg aggaaaaga 
      cagaaacat ttggacgag ttgacggat ttgacgcac 
      tctagttcg aatgatacg ttggattcg ttggagcac 
      gccgagttg atcgagaag gatagctag ggcattttt 
      ttgataggt tttttgagc aatattggt ttgtggttt 
      ggctggaag gtcaaatgt ctctcttga tgaaatcgt 
      gataaattt gttagttta tatttttgt attttttaa 
      agctcaggt tagggtact tagctttta aatgttttt 
      tattgttac cgagcatgt gttttatgt acataagag 
      tgcacatcg aataggacc aattgagcg tgccaactt 
      atttatttt atatttata gcacgtttc acaagtttt 
      cttaaagaa tattgttgg gataataaa caactggtt 
      gcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY