Status
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Gene information
General information
TC006614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11984041..11990201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006614
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01067

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late rounded - Number of animals showing the phenotype: 3

    vitellogenic egg chamber segmentation/division irregular - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006614 RA
      MASLWAGSD VDLSVLHQC RIAERLEFR LACLHKQKI 
PDSDTKCEG EIHKFRQCR RRSGTWPRT RSLNAPSPI 
QQFGPCGRI MKNSAMVII SSDCDISVS VVNFSEMVA 
NGNVVCLPF AEEINNNNN MKFQYRTIQ DPASQRLTW 
YKPPLTVLV IKKVRDASV HLPFVQLVQ WLIEEKRML 
VFVEAAVLE DPLLDQFTS FATIKDKLM TFRDGKDDL 
TDKIDFIIC LGGDGTLLY ASHLFQQSV PPVMAFHLG 
SLGFLTPFR FDNFQEQVN NVLEGNAAL TLRSRLRCI 
IMRKGDDEK KTSKQPTNL LVLNEVVVD RGPSPYLSN 
IDLFLDGKL ITSVQGDGL IVSTPTGST AYAVAAGAS 
MIHPSVPAI MVTPICPHS LSFRPIVVP AGVELKISV 
SRDSRNTSW VSFDGRNRQ ELLHGDSLR VTTSIYPVP 
SICAQDQIS DWFDSLAEC LHWNVRKRQ KHLDELTDL 
THSSSNDTL DSLEHAELI EKDS
    CDS sequence
    • >TC006614 RA
      atggcatcg ctatgggcc ggttcggat gtggatttg 
tccgttttg caccagtgc aggatagcc gaaaggtta 
gagttcaga ttggcctgt ttgcataaa cagaaaatc 
cctgattct gatacgaaa tgcgagggg gaaattcac 
aaattcagg cagtgcagg agacggtca ggcacttgg 
ccgaggacg aggtctctg aatgcaccg tcgcccata 
caacagttt ggaccatgt ggacgcatt atgaaaaat 
tccgcaatg gtcattatt agctcagac tgtgatatt 
agtgtgtca gtggtaaat ttttccgaa atggtcgcg 
aacggaaac gtcgtttgt ctcccgttc gccgaagaa 
atcaacaac aataacaac atgaaattt cagtatagg 
accatccag gatccggcg agccaaagg ctgacgtgg 
tacaaaccg ccacttact gtcctagtg ataaaaaaa 
gtgagggat gcctcggtc catttgccg tttgtgcag 
ctggtgcaa tggcttata gaggaaaaa cggatgctt 
gtgttcgtg gaggcggcc gtcctggag gaccccctc 
cttgaccag ttcacctcc tttgccacc atcaaagac 
aagctgatg acgttccgc gacggcaag gacgatctc 
accgacaaa atcgacttt attatctgt ctcggcggt 
gacggcacc ctcctctac gccagccac cttttccag 
caatctgtg ccccctgtc atggccttc cacctcggt 
tccttgggg ttcctcacc cctttccgc ttcgacaac 
ttccaagag caagtcaac aacgtgctc gaaggcaac 
gccgcctta actctccga agtcgcttg cgatgtatc 
atcatgcga aagggtgac gacgagaag aagacgtcc 
aagcagccg acgaatctc ctggtgctc aacgaggtt 
gtcgttgat cgaggaccc tcaccttac ctctcaaac 
atcgatctt ttcctcgat ggaaaattg atcacttcg 
gtgcaaggg gacgggctg atcgtgagc acgcccact 
ggaagcacg gcctatgcc gtggcggct ggcgcgtcc 
atgatccat ccgagcgtg ccggcgatc atggtcacg 
cccatttgc ccccactcg ttgagtttc cggccaatt 
gtcgtgccg gcgggggtg gagttaaag atttctgtc 
tctcgtgac agtcgcaat acgtcatgg gtgtcgttt 
gacggccgc aaccgccaa gagctgtta catggtgac 
agtttgcgt gtgacaacg tcgatttat ccagtgccg 
agcatttgc gcacaagac cagatttcc gattggttc 
gattcgctg gctgagtgt ctgcactgg aacgtgagg 
aaaagacag aaacatttg gacgagttg acggatttg 
acgcactct agttcgaat gatacgttg gattcgttg 
gagcacgcc gagttgatc gagaaggat agctag
    mRNA sequence
    • >TC006614 RA
      ttgtttaaa aatagaaaa ttttgggat ggcccatta 
atttgaaat aaaatccgg gttgttgcc gtcacctgt 
cattaaagc tgtcatttg tcaaatttt cccaaaaat 
gacgtgtaa caacaaaaa cccgaaacc gtcgtgaaa 
ttcctcgtt ggtgtgtga aagtgtgcc cttttcggt 
cttccattc tcgctccta gtgcggtcc tgcgtctca 
tatctgtca aaatgtcaa agtctttga cctaaccta 
gtttcaata tttgcgaaa taaaggcgt attatggca 
tcgctatgg gccggttcg gatgtggat ttgtccgtt 
ttgcaccag tgcaggata gccgaaagg ttagagttc 
agattggcc tgtttgcat aaacagaaa atccctgat 
tctgatacg aaatgcgag ggggaaatt cacaaattc 
aggcagtgc aggagacgg tcaggcact tggccgagg 
acgaggtct ctgaatgca ccgtcgccc atacaacag 
tttggacca tgtggacgc attatgaaa aattccgca 
atggtcatt attagctca gactgtgat attagtgtg 
tcagtggta aatttttcc gaaatggtc gcgaacgga 
aacgtcgtt tgtctcccg ttcgccgaa gaaatcaac 
aacaataac aacatgaaa tttcagtat aggaccatc 
caggatccg gcgagccaa aggctgacg tggtacaaa 
ccgccactt actgtccta gtgataaaa aaagtgagg 
gatgcctcg gtccatttg ccgtttgtg cagctggtg 
caatggctt atagaggaa aaacggatg cttgtgttc 
gtggaggcg gccgtcctg gaggacccc ctccttgac 
cagttcacc tcctttgcc accatcaaa gacaagctg 
atgacgttc cgcgacggc aaggacgat ctcaccgac 
aaaatcgac tttattatc tgtctcggc ggtgacggc 
accctcctc tacgccagc caccttttc cagcaatct 
gtgccccct gtcatggcc ttccacctc ggttccttg 
gggttcctc acccctttc cgcttcgac aacttccaa 
gagcaagtc aacaacgtg ctcgaaggc aacgccgcc 
ttaactctc cgaagtcgc ttgcgatgt atcatcatg 
cgaaagggt gacgacgag aagaagacg tccaagcag 
ccgacgaat ctcctggtg ctcaacgag gttgtcgtt 
gatcgagga ccctcacct tacctctca aacatcgat 
cttttcctc gatggaaaa ttgatcact tcggtgcaa 
ggggacggg ctgatcgtg agcacgccc actggaagc 
acggcctat gccgtggcg gctggcgcg tccatgatc 
catccgagc gtgccggcg atcatggtc acgcccatt 
tgcccccac tcgttgagt ttccggcca attgtcgtg 
ccggcgggg gtggagtta aagatttct gtctctcgt 
gacagtcgc aatacgtca tgggtgtcg tttgacggc 
cgcaaccgc caagagctg ttacatggt gacagtttg 
cgtgtgaca acgtcgatt tatccagtg ccgagcatt 
tgcgcacaa gaccagatt tccgattgg ttcgattcg 
ctggctgag tgtctgcac tggaacgtg aggaaaaga 
cagaaacat ttggacgag ttgacggat ttgacgcac 
tctagttcg aatgatacg ttggattcg ttggagcac 
gccgagttg atcgagaag gatagctag ggcattttt 
ttgataggt tttttgagc aatattggt ttgtggttt 
ggctggaag gtcaaatgt ctctcttga tgaaatcgt 
gataaattt gttagttta tatttttgt attttttaa 
agctcaggt tagggtact tagctttta aatgttttt 
tattgttac cgagcatgt gttttatgt acataagag 
tgcacatcg aataggacc aattgagcg tgccaactt 
atttatttt atatttata gcacgtttc acaagtttt 
cttaaagaa tattgttgg gataataaa caactggtt 
gcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY