Status
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Gene information
General information
TC034135
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006612,TC006613
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11972081..11977999
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034135
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034135 RA
      MIVIRRRWL ALSLSGLAV VFFGFEFLQ TVGEGNKGL 
      ESGDSRKIV AFSLKKQIE IESEEVACS GGFFVGVKP 
      EKLANGKWT QLDGTSIFI YSIYFDGRL EPHHYLRTI 
      SMVKGTPGK EFFCQIATS DGHYHITKA VVTKIWSET 
      WNQNDTHLY FNPYLISCH LPVNKTSVY FSLNEKPCQ 
      IAPQKFVIT TPNVENTTS FTVCVKPLN FRNDISEQL 
      LQWIAINRI LGAEKIDFY VENLQKKPR QVLETLEKH 
      LNGGLTLYA HNQIAQEPT FKHSTHIWQ KRRYEIIAY 
      NDCLYRNIH TSHFVIPLD IDEIIVPRH GSTWAEILN 
      RTDDTFASF TVRNAYYLR HFNVKKSTE EVFFFKRTV 
      RSEFSDEGE SGKSFVSTK NALTVFNHY ALRSLKPGV 
      GVVKFLNVG LAQLNHYKD DCDTVILPE CAKYLSSPV 
      RVADGVIFK FRRLFFADR AKNHPKLTK TATMVTEEP 
      TESPLVDET KPKPKPEPL KPFTLEILK VIKNAQQQH 
      GLRHGDFQR YRGYCSRRI RRLRKVLKL PQGDRRHFK 
      KRDVTDSHI TDKKADERY LEIPLMLSE RCWAYAMQL 
      RQEANTEPR KKFHLIQKL RKACVYALQ LEELCQQER 
      CDARTKLES QAYVAWIQG SLQFELQIW LKATENLKK 
      AQMIYEKLG SALAENEQA PYKQRVVEI TPSLRYCAY 
      NIGDEKAVD LLELRSQGV LDNFDALVS QSKQKTEAV 
      LHEVQWFGI KIPVRIERV RLFLLSIEG LDESLIHAE 
      NNQAKIKIL ENLFIDLRD IISLARDVV RTEKQDQLL 
      LSYLLSIRV ERTSQRHLL LMQQTRKSQ DCVRLLDII 
      IQQTGELSQ NESIKDVKL AQDYYNQQL QAFKALRVY 
      FLAKTQAAQ KRFKEAAVL YDSASKALA KLPIKNKFL 
      KELDDLVKS VRDTVDVDK ITAQANCVL DQQEDQIQV 
      PQKVYKSKK PLIERLDEF REEPQLLSK NPNIVSLPP 
      AMEPIPAKP LFYDLALNF VELPDLTEK LEGGQKKEK 
      GAGISGFVK GLWGWGKK
    CDS sequence
    • >TC034135 RA
      atgatagtc attaggaga cgctggctg gctttgagt 
      ttgagtggc ttggcagtg gtttttttc ggttttgag 
      tttctccaa acagttggg gagggtaat aaggggctg 
      gaaagtggg gacagcagg aaaattgta gctttttct 
      ttgaagaaa caaatcgaa attgaaagt gaagaggtg 
      gcttgtagt ggcgggttt ttcgttggg gtaaagccc 
      gaaaaattg gcaaatgga aagtggaca caattagac 
      gggacgagt atttttatt tattcgatt tattttgat 
      ggcaggctg gagccgcat cattattta aggacaata 
      tcaatggtt aaaggcact cctggtaaa gaatttttt 
      tgccaaatt gccacctct gatggtcac taccacatc 
      accaaagcc gtagtaacg aaaatttgg tccgaaaca 
      tggaaccaa aacgacact catttgtac ttcaacccc 
      tatctcata tcttgccat ttacccgtg aataaaacc 
      tcggtttat ttctccctc aatgaaaag ccgtgtcaa 
      atagcgccc caaaaattt gtgataaca acccccaat 
      gtggaaaac acaactagc tttacagtt tgtgtaaaa 
      ccactaaat tttcggaac gacatttcc gaacaactc 
      ctccagtgg atcgcaatc aaccgaata ctgggggcc 
      gaaaaaatc gacttttac gttgaaaat ctccaaaaa 
      aagccgcgc caagttctt gaaactctg gaaaagcac 
      ttgaacggt ggtttaact ctgtatgcc cataaccag 
      atcgctcaa gagccaact ttcaagcac tcaacccac 
      atttggcaa aaaagacgt tacgaaatc atcgcctac 
      aacgactgc ctttaccgc aacatccac acgtcacac 
      tttgtaatt cctctcgac attgacgaa ataattgta 
      ccaagacat ggaagcacg tgggcggaa attcttaac 
      cgaactgac gacactttt gcctcgttt actgttaga 
      aatgcctat tatttgcgc cactttaac gtgaaaaaa 
      tcaacagag gaggtgttt ttcttcaaa aggacggtc 
      cggagcgag ttcagcgac gagggagag tcggggaaa 
      agcttcgtt tccaccaaa aatgcacta acagtgttc 
      aatcactac gccttaagg agtttaaag ccgggggtt 
      ggggtggtg aagttttta aacgttggt ttagcgcag 
      cttaaccat tataaggat gactgtgat actgtgata 
      ttgccggag tgtgccaaa tatttgtct agtcctgta 
      agggtcgct gatggtgtt attttcaag tttaggagg 
      ctgtttttt gcagatcgt gcaaaaaat cacccaaaa 
      ttaacaaaa actgcaact atggtcacc gaagagccc 
      accgaaagc cccctcgtc gacgagact aaacccaaa 
      ccaaaaccg gagccccta aaacccttc actcttgaa 
      atcctcaaa gtgatcaaa aacgcccaa caacagcac 
      ggcctccgc cacggcgac ttccagcgc taccgtggc 
      tactgctcc cgccgcatc cggcgcctc cgcaaggtc 
      ctcaagctg ccgcaaggc gaccgccgc cacttcaaa 
      aaacgcgac gttacagac tctcacatt accgacaaa 
      aaagccgac gagcgctat ctcgaaatc cccctaatg 
      ctctccgag cgctgctgg gcctatgcc atgcaactc 
      cgccaagaa gccaacacc gaaccccgc aaaaaattc 
      cacctaatc cagaaacta cgcaaggct tgcgtctac 
      gccctccag ctggaggag ttgtgccag caggagcgc 
      tgcgatgcc aggaccaag ctcgagtcg caagcctac 
      gtggcctgg atccagggg agtctccag ttcgagctc 
      cagatctgg ctcaaagcc accgaaaat ctcaaaaaa 
      gcgcaaatg atttacgag aagttgggg tccgctttg 
      gcggagaac gaacaagcg ccgtacaag cagcgagtt 
      gttgaaatt acacccagt ttgcgctac tgcgcctat 
      aatataggg gacgagaaa gctgttgat ttgctagaa 
      ctgaggagt caaggcgtt ttggataat ttcgacgct 
      ttggtgtcg caatctaag caaaaaacc gaggctgtt 
      ttacacgaa gtgcaatgg ttcgggatt aaaatcccg 
      gtgaggatt gaacgcgtc cgcttgttt ctcttgagt 
      attgaaggc ttggacgag tctttaatc cacgcagaa 
      aacaaccaa gcgaaaatt aaaattctc gagaatttg 
      tttatcgac ttgcgggat attatttca ctagcgcgc 
      gatgttgtc agaactgag aaacaagac caattgtta 
      ctgtcttat ttattatcg attcgggtc gagagaacg 
      tctcagaga cacttgtta ctgatgcaa caaacgcgc 
      aaatcgcaa gattgcgtc cgtttactt gacataata 
      atccaacaa acgggcgaa ttgtcgcag aacgagtca 
      atcaaagac gtgaaactg gcccaggat tattacaac 
      cagcagttg caagcgttc aaggctttg cgcgtctat 
      ttcctggcg aaaacacaa gcggcccaa aaacgcttc 
      aaggaagcg gccgttttg tacgattcc gcctccaaa 
      gccctcgct aaactccca ataaaaaat aaatttctc 
      aaagaactg gacgattta gtgaagagt gtgcgagac 
      actgtcgat gttgataaa atcacagcg caggccaac 
      tgtgtgttg gaccagcaa gaggatcaa attcaagtg 
      ccccaaaag gtgtacaag agtaagaaa ccgttgatt 
      gagcgcttg gacgagttt agagaagag ccgcaattg 
      ttgagcaaa aacccgaat attgtgagt ctgcctccg 
      gccatggaa ccgattcct gccaagccg ttgttttat 
      gatttggca ttgaatttc gtcgagtta cccgatttg 
      acggagaaa ttggaaggc gggcagaag aaggaaaag 
      ggggcgggg atttccggg ttcgtcaag gggctgtgg 
      gggtgggga aagaagtaa
    mRNA sequence
    • >TC034135 RA
      ggagagtgg catgatagt cattaggag acgctggct 
      ggctttgag tttgagtgg cttggcagt ggttttttt 
      cggttttga gtttctcca aacagttgg ggagggtaa 
      taaggggct ggaaagtgg ggacagcag gaaaattgt 
      agctttttc tttgaagaa acaaatcga aattgaaag 
      tgaagaggt ggcttgtag tggcgggtt tttcgttgg 
      ggtaaagcc cgaaaaatt ggcaaatgg aaagtggac 
      acaattaga cgggacgag tatttttat ttattcgat 
      ttattttga tggcaggct ggagccgca tcattattt 
      aaggacaat atcaatggt taaaggcac tcctggtaa 
      agaattttt ttgccaaat tgccacctc tgatggtca 
      ctaccacat caccaaagc cgtagtaac gaaaatttg 
      gtccgaaac atggaacca aaacgacac tcatttgta 
      cttcaaccc ctatctcat atcttgcca tttacccgt 
      gaataaaac ctcggttta tttctccct caatgaaaa 
      gccgtgtca aatagcgcc ccaaaaatt tgtgataac 
      aacccccaa tgtggaaaa cacaactag ctttacagt 
      ttgtgtaaa accactaaa ttttcggaa cgacatttc 
      cgaacaact cctccagtg gatcgcaat caaccgaat 
      actgggggc cgaaaaaat cgactttta cgttgaaaa 
      tctccaaaa aaagccgcg ccaagttct tgaaactct 
      ggaaaagca cttgaacgg tggtttaac tctgtatgc 
      ccataacca gatcgctca agagccaac tttcaagca 
      ctcaaccca catttggca aaaaagacg ttacgaaat 
      catcgccta caacgactg cctttaccg caacatcca 
      cacgtcaca ctttgtaat tcctctcga cattgacga 
      aataattgt accaagaca tggaagcac gtgggcgga 
      aattcttaa ccgaactga cgacacttt tgcctcgtt 
      tactgttag aaatgccta ttatttgcg ccactttaa 
      cgtgaaaaa atcaacaga ggaggtgtt tttcttcaa 
      aaggacggt ccggagcga gttcagcga cgagggaga 
      gtcggggaa aagcttcgt ttccaccaa aaatgcact 
      aacagtgtt caatcacta cgccttaag gagtttaaa 
      gccgggggt tggggtggt gaagttttt aaacgttgg 
      tttagcgca gcttaacca ttataagga tgactgtga 
      tactgtgat attgccgga gtgtgccaa atatttgtc 
      tagtcctgt aagggtcgc tgatggtgt tattttcaa 
      gtttaggag gctgttttt tgcagatcg tgcaaaaaa 
      tcacccaaa attaacaaa aactgcaac tatggtcac 
      cgaagagcc caccgaaag ccccctcgt cgacgagac 
      taaacccaa accaaaacc ggagcccct aaaaccctt 
      cactcttga aatcctcaa agtgatcaa aaacgccca 
      acaacagca cggcctccg ccacggcga cttccagcg 
      ctaccgtgg ctactgctc ccgccgcat ccggcgcct 
      ccgcaaggt cctcaagct gccgcaagg cgaccgccg 
      ccacttcaa aaaacgcga cgttacaga ctctcacat 
      taccgacaa aaaagccga cgagcgcta tctcgaaat 
      ccccctaat gctctccga gcgctgctg ggcctatgc 
      catgcaact ccgccaaga agccaacac cgaaccccg 
      caaaaaatt ccacctaat ccagaaact acgcaaggc 
      ttgcgtcta cgccctcca gctggagga gttgtgcca 
      gcaggagcg ctgcgatgc caggaccaa gctcgagtc 
      gcaagccta cgtggcctg gatccaggg gagtctcca 
      gttcgagct ccagatctg gctcaaagc caccgaaaa 
      tctcaaaaa agcgcaaat gatttacga gaagttggg 
      gtccgcttt ggcggagaa cgaacaagc gccgtacaa 
      gcagcgagt tgttgaaat tacacccag tttgcgcta 
      ctgcgccta taatatagg ggacgagaa agctgttga 
      tttgctaga actgaggag tcaaggcgt tttggataa 
      tttcgacgc tttggtgtc gcaatctaa gcaaaaaac 
      cgaggctgt tttacacga agtgcaatg gttcgggat 
      taaaatccc ggtgaggat tgaacgcgt ccgcttgtt 
      tctcttgag tattgaagg cttggacga gtctttaat 
      ccacgcaga aaacaacca agcgaaaat taaaattct 
      cgagaattt gtttatcga cttgcggga tattatttc 
      actagcgcg cgatgttgt cagaactga gaaacaaga 
      ccaattgtt actgtctta tttattatc gattcgggt 
      cgagagaac gtctcagag acacttgtt actgatgca 
      acaaacgcg caaatcgca agattgcgt ccgtttact 
      tgacataat aatccaaca aacgggcga attgtcgca 
      gaacgagtc aatcaaaga cgtgaaact ggcccagga 
      ttattacaa ccagcagtt gcaagcgtt caaggcttt 
      gcgcgtcta tttcctggc gaaaacaca agcggccca 
      aaaacgctt caaggaagc ggccgtttt gtacgattc 
      cgcctccaa agccctcgc taaactccc aataaaaaa 
      taaatttct caaagaact ggacgattt agtgaagag 
      tgtgcgaga cactgtcga tgttgataa aatcacagc 
      gcaggccaa ctgtgtgtt ggaccagca agaggatca 
      aattcaagt gccccaaaa ggtgtacaa gagtaagaa 
      accgttgat tgagcgctt ggacgagtt tagagaaga 
      gccgcaatt gttgagcaa aaacccgaa tattgtgag 
      tctgcctcc ggccatgga accgattcc tgccaagcc 
      gttgtttta tgatttggc attgaattt cgtcgagtt 
      acccgattt gacggagaa attggaagg cgggcagaa 
      gaaggaaaa gggggcggg gatttccgg gttcgtcaa 
      ggggctgtg ggggtgggg aaagaagta aggagtcgc 
      acttagcga tcgtaatat gtcgccatc tgttggttt 
      atttcttgt tactacacc gtaaattat taatttacc 
      tctgcggtg acattaaat tatgtaaga atggtgact 
      gccattttt ttttaatgg cgtcttgtg tattttttg 
      caaaaaaaa gaaacttgc ttcagagcc caaggaaac 
      cgtggtatt aggggatta ggttaattt ttatcattc 
      ggaaataaa gtgtaatgc agaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY