Status
Please wait ...
Gene information
General information
TC006611 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11964391..11967352
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006611
    iBeetle screen iB_01065

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Many very different phenotypes. Most of them known from the typical backround, no identifiable system behind the phenotypes (no common defects).
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006611 RA
      MQSMGLGGG FLMTIYIKD QNKAYTLNA REKAPLKAT 
      PDLYKDDQF ISRKGALAI AVPGELRGY QAAHEKFGK 
      LEWSKLVEP SIELCEKGY NMSKHQHES LKEARIVND 
      TNLKEWFFD SEGKFKKMG STIVPKKLC ETLRLIAKS 
      GGHDLYNGT LSKMLVEDL EEIGSIITK EDLESYQAE 
      WMDPIEVEL RHHEKLYSV PPPGSGALL AFILSILDG 
      FKFKPHDLE GVQNTVQTY HKIIEAFKY AYAKRTELG 
      DTNFVHISD LLRNLTSHE YAESIRKEI QENSTSNDP 
      KDYGAVFYS KEDHGTAHI SVLAENGDA VSVTSSVNL 
      YFGAGLTSR QTGIILNSV MDDFSFPNF QNYFNLPGS 
      PNNELRPGK RPLSSMSPT ILVDEHGDV KLVLGASGG 
      TKITTSVAL VIMRTLWFG QNIKEAIDA PRIHHQLYP 
      MQVQYEYGT LQQVVTGLE ALGHKTSRY TDAGSIICA 
      LYNTSGLIQ ANADHRKGG DVYGL
    CDS sequence
    • >TC006611 RA
      atgcagagt atgggtctt gggggcggc ttcctcatg 
      accatctac atcaaagac caaaacaaa gcctacact 
      ctaaacgct cgcgaaaaa gcccccttg aaagccaca 
      cccgacttg tacaaagac gaccaattt atttcccga 
      aaaggggct ctagccatc gcagtgccc ggcgaactc 
      aggggctat caagcggcc cacgaaaaa ttcggcaaa 
      ctcgaatgg tcaaagctg gtcgaaccc agcatagaa 
      ttgtgcgaa aaaggctac aacatgtcc aaacaccag 
      cacgaatcc ttgaaagag gccaggatt gtgaacgat 
      acgaatttg aaggagtgg tttttcgat tctgagggt 
      aaattcaaa aaaatgggc tcgacaata gttccgaaa 
      aaattatgt gagacgtta cgattgatt gcgaaaagt 
      ggcggtcac gacctttac aatggcact ttaagcaag 
      atgttggta gaagacttg gaggaaatc ggtagtatt 
      ataaccaaa gaagattta gaaagttac caggccgaa 
      tggatggac cccatcgaa gtggagctc cgccaccac 
      gaaaaactc tattccgtt ccgccacca ggctcaggg 
      gcccttcta gcgtttatt ttaagcatc ctggatggg 
      ttcaaattc aaaccacat gatctggaa ggcgttcag 
      aacacagtt cagacgtac cacaaaata atcgaggct 
      tttaaatac gcgtacgcc aaaagaact gaattaggt 
      gatactaac tttgtacac atttctgat ttattacgt 
      aatttaact tcgcacgaa tatgccgaa agtatcagg 
      aaggaaatt caggaaaat tcgaccagt aatgatccc 
      aaagactac ggagcggtt ttttacagc aaggaggac 
      catggcaca gctcatatc tccgttctg gccgaaaat 
      ggggacgca gtctcggtc acaagctcc gtcaatttg 
      tatttcggg gccggtctg acgtcgcgc caaacgggc 
      attattctg aacagtgtc atggacgat ttttcattc 
      cccaatttc caaaattat ttcaatttg cctggatct 
      cccaacaac gaactgagg cctggaaaa cggccgttg 
      tcctcaatg agccccact atcctggtg gacgagcac 
      ggggacgta aaactggtt ctgggggct tcggggggc 
      acgaaaatc accacatct gtggcgctg gtaattatg 
      cgcacgctc tggttcggc cagaacatc aaagaggcc 
      atcgacgcc cctcgaatc caccaccag ctctaccca 
      atgcaagtt cagtacgaa tatggcaca ttacaacaa 
      gtagtcact ggtttggag gccctaggg cacaagact 
      agccgttac accgacgca ggctccata atttgtgcc 
      ctttacaac acctcaggc ctaatccaa gccaacgct 
      gaccaccgc aaaggcggc gatgtttac ggactgtga
    mRNA sequence
    • >TC006611 RA
      gtaattttt gaaaaatat tttttagat caatcctag 
      accagaatg gcagtgctg tggacgccg ccatcgcca 
      cgttattct gcaacggga tttacacca tgcagagta 
      tgggtcttg ggggcggct tcctcatga ccatctaca 
      tcaaagacc aaaacaaag cctacactc taaacgctc 
      gcgaaaaag cccccttga aagccacac ccgacttgt 
      acaaagacg accaattta tttcccgaa aaggggctc 
      tagccatcg cagtgcccg gcgaactca ggggctatc 
      aagcggccc acgaaaaat tcggcaaac tcgaatggt 
      caaagctgg tcgaaccca gcatagaat tgtgcgaaa 
      aaggctaca acatgtcca aacaccagc acgaatcct 
      tgaaagagg ccaggattg tgaacgata cgaatttga 
      aggagtggt ttttcgatt ctgagggta aattcaaaa 
      aaatgggct cgacaatag ttccgaaaa aattatgtg 
      agacgttac gattgattg cgaaaagtg gcggtcacg 
      acctttaca atggcactt taagcaaga tgttggtag 
      aagacttgg aggaaatcg gtagtatta taaccaaag 
      aagatttag aaagttacc aggccgaat ggatggacc 
      ccatcgaag tggagctcc gccaccacg aaaaactct 
      attccgttc cgccaccag gctcagggg cccttctag 
      cgtttattt taagcatcc tggatgggt tcaaattca 
      aaccacatg atctggaag gcgttcaga acacagttc 
      agacgtacc acaaaataa tcgaggctt ttaaatacg 
      cgtacgcca aaagaactg aattaggtg atactaact 
      ttgtacaca tttctgatt tattacgta atttaactt 
      cgcacgaat atgccgaaa gtatcagga aggaaattc 
      aggaaaatt cgaccagta atgatccca aagactacg 
      gagcggttt tttacagca aggaggacc atggcacag 
      ctcatatct ccgttctgg ccgaaaatg gggacgcag 
      tctcggtca caagctccg tcaatttgt atttcgggg 
      ccggtctga cgtcgcgcc aaacgggca ttattctga 
      acagtgtca tggacgatt tttcattcc ccaatttcc 
      aaaattatt tcaatttgc ctggatctc ccaacaacg 
      aactgaggc ctggaaaac ggccgttgt cctcaatga 
      gccccacta tcctggtgg acgagcacg gggacgtaa 
      aactggttc tgggggctt cggggggca cgaaaatca 
      ccacatctg tggcgctgg taattatgc gcacgctct 
      ggttcggcc agaacatca aagaggcca tcgacgccc 
      ctcgaatcc accaccagc tctacccaa tgcaagttc 
      agtacgaat atggcacat tacaacaag tagtcactg 
      gtttggagg ccctagggc acaagacta gccgttaca 
      ccgacgcag gctccataa tttgtgccc tttacaaca 
      cctcaggcc taatccaag ccaacgctg accaccgca 
      aaggcggcg atgtttacg gactgtgaa ttgttttgt 
      tgcacttcc cctgttttc gccattaat aaattctaa 
      tttagtga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY