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Gene information
General information
TC006555 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 4324689..4327913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006555
    iBeetle screen iB_01062
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006555 RA
      MGKTGLLVL LVIWARVGA ENGKQLNVL LILADDGGF 
      EMGAYRNKI CQTPNLDAL AKNSLIFNN AYTSVSSCS 
      PSRSALLTG MPAHQNGMY GLHQAENHF DSFTNVKSL 
      PNILRENGI RTGIIGKKH VGPKSTYRF DYEQTEENN 
      SILQVGRNI TLIKLLARE FLNNSTDKP FFLYVAFHD 
      PHRCGHTHP EYGQFCQRF GNGDVGMGL IPDWRPIYY 
      QWDELELPY YIPDTEAAR REVANQYTT ISRLDQGVG 
      LILEELEKS GHADDTLVI YSSDNGTPF PNGRTNLYD 
      SGIAEPMFI SSPLHKERH NQVTYSLTS LLDIVPTVL 
      DWFNITEES NEINSKKLT GKSLLPLLV KEPENTTNE 
      AIFASHNLH EVTMYYPMR MIRTHRYKL IHNLNYQAP 
      FPIDQDFYL SPTFQDILN RTRNKENLY WFKTLRQYY 
      NRPEWELYD LKHDPVELN NLAGKSDYK DIMQELEKR 
      LSEWQNATS DPWICSPHS VLENKGDYA HNPQCMPLY 
      NT
    CDS sequence
    • >TC006555 RA
      atggggaaa accggtttg cttgttcta ctagtgatt 
      tgggcacga gttggggct gaaaatggc aaacaatta 
      aatgtgctg ctgattttg gccgacgat ggcggcttc 
      gaaatgggg gcttacagg aacaaaatc tgtcaaacg 
      cccaatttg gacgccttg gccaaaaac agcctaatt 
      ttcaataat gcctacacc tcggtcagt agttgttcg 
      cccagccgt tcggcccta cttaccggc atgccggcc 
      catcagaat ggcatgtac ggcctccac caggccgaa 
      aaccacttc gattccttc acgaacgtg aaaagtttg 
      ccgaacatt ttgcgcgaa aacggcatt cgcaccgga 
      attatcggt aaaaaacac gtgggcccc aaaagtacg 
      tatcgtttc gactacgag cagactgag gagaataat 
      tcaatcctg caagtcggg cgcaatatc actttgatt 
      aaattgctg gccagagag ttcctcaac aattccacg 
      gacaagcca tttttcctc tatgttgcg ttccatgac 
      ccccatcgt tgcggccac acgcacccg gaatacggc 
      cagttctgc caacggttc ggcaacggg gacgtgggc 
      atgggtctg atcccggac tggcgccca atttattac 
      caatgggac gaacttgag ctaccttat tatattccc 
      gatacggag gcggcgagg cgtgaagtg gccaatcaa 
      tacacgaca atctcgaga ttggatcaa ggcgttggt 
      ttaattttg gaagaattg gaaaaatca ggtcacgct 
      gatgatact cttgtgatt tacagttcg gataatggg 
      accccattt ccgaacgga cgcacgaat ttatacgat 
      tcggggatt gccgaaccc atgtttatt tcctcgcct 
      ttgcacaag gaaaggcac aatcaggtc acctactcc 
      ttgaccagc ctcctagat attgtacca actgtcctt 
      gattggttc aatatcaca gaggagagc aacgaaatc 
      aatagtaaa aaactgacc gggaaaagc ttattgcca 
      cttctagtt aaagagccg gaaaataca acaaacgag 
      gcgattttc gcgagtcat aatctccat gaagtaacg 
      atgtattac ccaatgagg atgatcaga acccaccgc 
      tataaattg attcataat ttgaattac caagcgccg 
      ttcccgatc gatcaggac ttttactta tcgccaaca 
      ttccaagat attttgaac cgcaccaga aacaaggaa 
      aatctgtac tggtttaag accctgaga cagtactac 
      aaccggccc gagtgggaa ctgtacgac ttgaaacac 
      gaccccgtg gaactaaac aatctggct ggaaagagc 
      gactacaaa gacatcatg caggagctg gagaaacga 
      ctttccgag tggcaaaac gccacaagt gacccgtgg 
      atttgctcc ccacattca gtcttggaa aataagggt 
      gactatgca cacaacccc caatgcatg cccctgtac 
      aacacgtaa
    mRNA sequence
    • >TC006555 RA
      cccgaattt atactgcac gagcccatt ttccgggaa 
      gacgtagtc gaatctgaa aatgacgtc ctcgccgtt 
      catttgctc ggcgatttt cttgaaatc ggagccccc 
      gaccaggcc gagggtgca caagggctt gattttcgt 
      ttggatgaa ctcgtcgaa ttcggggtc gattgcttg 
      gcggggttt tgtgagggt gccgtccat gtcgaataa 
      acacaaaat tttctttgg tggcatgtt tgaatttgg 
      ctccgttgt gtgaaaaga tggggaaaa ccggtttgc 
      ttgttctac tagtgattt gggcacgag ttggggctg 
      aaaatggca aacaattaa atgtgctgc tgattttgg 
      ccgacgatg gcggcttcg aaatggggg cttacagga 
      acaaaatct gtcaaacgc ccaatttgg acgccttgg 
      ccaaaaaca gcctaattt tcaataatg cctacacct 
      cggtcagta gttgttcgc ccagccgtt cggccctac 
      ttaccggca tgccggccc atcagaatg gcatgtacg 
      gcctccacc aggccgaaa accacttcg attccttca 
      cgaacgtga aaagtttgc cgaacattt tgcgcgaaa 
      acggcattc gcaccggaa ttatcggta aaaaacacg 
      tgggcccca aaagtacgt atcgtttcg actacgagc 
      agactgagg agaataatt caatcctgc aagtcgggc 
      gcaatatca ctttgatta aattgctgg ccagagagt 
      tcctcaaca attccacgg acaagccat ttttcctct 
      atgttgcgt tccatgacc cccatcgtt gcggccaca 
      cgcacccgg aatacggcc agttctgcc aacggttcg 
      gcaacgggg acgtgggca tgggtctga tcccggact 
      ggcgcccaa tttattacc aatgggacg aacttgagc 
      taccttatt atattcccg atacggagg cggcgaggc 
      gtgaagtgg ccaatcaat acacgacaa tctcgagat 
      tggatcaag gcgttggtt taattttgg aagaattgg 
      aaaaatcag gtcacgctg atgatactc ttgtgattt 
      acagttcgg ataatggga ccccatttc cgaacggac 
      gcacgaatt tatacgatt cggggattg ccgaaccca 
      tgtttattt cctcgcctt tgcacaagg aaaggcaca 
      atcaggtca cctactcct tgaccagcc tcctagata 
      ttgtaccaa ctgtccttg attggttca atatcacag 
      aggagagca acgaaatca atagtaaaa aactgaccg 
      ggaaaagct tattgccac ttctagtta aagagccgg 
      aaaatacaa caaacgagg cgattttcg cgagtcata 
      atctccatg aagtaacga tgtattacc caatgagga 
      tgatcagaa cccaccgct ataaattga ttcataatt 
      tgaattacc aagcgccgt tcccgatcg atcaggact 
      tttacttat cgccaacat tccaagata ttttgaacc 
      gcaccagaa acaaggaaa atctgtact ggtttaaga 
      ccctgagac agtactaca accggcccg agtgggaac 
      tgtacgact tgaaacacg accccgtgg aactaaaca 
      atctggctg gaaagagcg actacaaag acatcatgc 
      aggagctgg agaaacgac tttccgagt ggcaaaacg 
      ccacaagtg acccgtgga tttgctccc cacattcag 
      tcttggaaa ataagggtg actatgcac acaaccccc 
      aatgcatgc ccctgtaca acacgtaaa cacaaaaat 
      atatttatt gtcacatat tctaaacaa atgcgtata 
      taaaacatc tacgtttgc atatggcga cttattccc 
      taattaact aatcacacg caacatttt aataaatta 
      gtcaacaac ggctagact tctccagtg ataacacaa 
      attaaaaac aaaagcgat aagtgtgaa ttgaccaag 
      cgcttgcgt tagaatatt ttacggaaa ttaccatca 
      cctcgtttt aattttttt taatctgcc cctaagcgt 
      cctccgttt cacagtctt ttccaactt gctaactag 
      aaccgaagt ggtgttcgc gaccgcttc gacactgtg 
      catagtttc tgcgatttc agtcatctg caaaattgg 
      gtttaattg cgtccaagg ccgctgatt gactcactc 
      tctcgtgga tctcttgta tccgtaaat gcttctggt 
      ggttgttat acaatacct taaattgta acataaaac 
      ccgcggaca aaatcaaca cacaaaata tcagcaaat 
      tatttgact gccggaaat gccacatca ctgttgtaa 
      ttcatttta gcagtgttt gagctgcaa tcaagcaat 
      tagcactga catcaacac aaacacctt acttaacaa 
      gggatttaa ttacatcaa gagaatttg taaaca