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Gene information
General information
TC006554 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 4323259..4324633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006554
    iBeetle screen iB_01061
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006554 RA
      MFAIRVAQN VLRPQRRKL SQVAQDLTY TQLQGSDTG 
      ITVFGFNRP KQKNALSAN LVTELQKSL QKITFDPSA 
      RVLIVRSHV PGVFCAGAD LKERATMPQ AEVGKFVSN 
      LRSLMDQFH NLPIPVIAA LDGVALGGG LEISLACDI 
      RVASSDCKM GLVEGKLAI IPGAGGTQR LPRLINPSI 
      AKELIYTAR TIDGGTAHR LGIVNHVVS QNANQDAAY 
      LKSLELAHE ILPNGPVAV QMAKKAINK GVQVDLSSG 
      LAIEEACYA QLIPTKDRL EGLQAFKEK RKPNYIGE
    CDS sequence
    • >TC006554 RA
      atgttcgca ataagagtg gcacaaaat gtgttgcgc 
      ccccaaaga cgcaaacta tcgcaagtc gcccaagac 
      ctgacctac acgcaactg caaggatct gacaccgga 
      ataaccgtt ttcggtttt aaccgcccc aaacaaaag 
      aacgcctta agtgccaac ctagtaacc gaattacag 
      aaatctctc cagaaaatc acattcgac ccctcggcc 
      cgcgtttta atagtgcga agccacgtc ccgggagtc 
      ttctgtgcc ggagcggat cttaaagaa cgcgcaacg 
      atgcctcaa gcggaagtc ggtaaattc gtctcaaac 
      ctgcgaagt ctcatggac cagttccac aacctccct 
      atccccgtc atagccgcc ttggatgga gttgctttg 
      gggggaggt ctggagata tccctggct tgtgacatt 
      cgggtcgct tcaagtgac tgtaaaatg gggctagtt 
      gagggcaaa ttggccata ataccgggg gccggaggc 
      acccaaaga ctcccccgg cttatcaac ccctcaatc 
      gccaaggaa ttgatatac acggcgcga acaatcgac 
      ggtggtacc gcccaccga ttgggtatt gtcaaccac 
      gtggtgtca caaaacgcc aatcaggac gcagcttat 
      ttgaaatca ctggagttg gcgcacgaa atattaccg 
      aatggacct gtggcggtc caaatggcc aaaaaggcc 
      atcaataag ggggttcaa gtcgactta agttcggga 
      ttagcgatc gaggaggcg tgctatgct cagctgata 
      ccgaccaag gacaggtta gagggacta caggcgttc 
      aaggagaag cgaaaaccg aattatatt ggcgaatga
    mRNA sequence
    • >TC006554 RA
      ctaaaatgt tcgcaataa gagtggcac aaaatgtgt 
      tgcgccccc aaagacgca aactatcgc aagtcgccc 
      aagacctga cctacacgc aactgcaag gatctgaca 
      ccggaataa ccgttttcg gttttaacc gccccaaac 
      aaaagaacg ccttaagtg ccaacctag taaccgaat 
      tacagaaat ctctccaga aaatcacat tcgacccct 
      cggcccgcg ttttaatag tgcgaagcc acgtcccgg 
      gagtcttct gtgccggag cggatctta aagaacgcg 
      caacgatgc ctcaagcgg aagtcggta aattcgtct 
      caaacctgc gaagtctca tggaccagt tccacaacc 
      tccctatcc ccgtcatag ccgccttgg atggagttg 
      ctttggggg gaggtctgg agatatccc tggcttgtg 
      acattcggg tcgcttcaa gtgactgta aaatggggc 
      tagttgagg gcaaattgg ccataatac cgggggccg 
      gaggcaccc aaagactcc cccggctta tcaacccct 
      caatcgcca aggaattga tatacacgg cgcgaacaa 
      tcgacggtg gtaccgccc accgattgg gtattgtca 
      accacgtgg tgtcacaaa acgccaatc aggacgcag 
      cttatttga aatcactgg agttggcgc acgaaatat 
      taccgaatg gacctgtgg cggtccaaa tggccaaaa 
      aggccatca ataaggggg ttcaagtcg acttaagtt 
      cgggattag cgatcgagg aggcgtgct atgctcagc 
      tgataccga ccaaggaca ggttagagg gactacagg 
      cgttcaagg agaagcgaa aaccgaatt atattggcg 
      aatgattaa aaaaaaatt aaagatcaa ataattttt 
      caagttgct ttcttgtat catccgggt ctgagacct 
      tgtggccta tgactctcg ggtcattgt agatttcgt 
      agtcgttgc ccccttcgt ccgtcttgt ccccaaaga 
      agtgaatct cgtcaaagc cctcgcttt ggacgtggc 
      gcagacaat acgtcttgt cccaccctt tggggaagg 
      cgtcgaagc tgatttgcc ccccgattg cgtacgtga 
      gccctatat cggggaatt tcttcctga gggcctcaa 
      tcatcgtct gtcttatgc aaaactcct tatcatatt 
      tctcgaaac tttcgcgct cttcctggg aacaggagc 
      gcccgactg gcgagatat tgagcatcc cggccctga 
      actcgacaa agttgcccc gtttgaccg gcaggacca 
      ccgtcgata agtagctca ggacgtagt ttatgaaca