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Gene information
General information
TC034118
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006545,TC006546
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11304810..11308713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034118
    iBeetle screen iB_01059

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034118 RA
      MILLETNNR VVEETLTVK IKSAQAGLK NESIDITVS 
      DFDGVLYHI SNLNGDKTK IRISISLKF YKELQEHGA 
      DQLLKREYG PLLAAPEDG YNVSILFDL ENIPTDWPQ 
      VIKKIGLLK RNCFASVFE KYFDFQENG DEGQKRAVI 
      HYRDDETMY VEAKADRVT VVFSTRFRD EDDVVIGKV 
      FMQELKEGL RASHTAPPV LFSHREPPR ELQNTDAKV 
      DNNVGYVTF VLFPRHTAR TTRDNTIDL IHMFRHYLH 
      YHIKCSKAY IHSRMRAKT SDFLKVLNR ARPENKSTD 
      KKTITYRNC FASVFEKYF DFQENGDEG QKRAVIHYR 
      DDETMYVEA KADRVTVVF STRFRDEDD VVIGKVFMQ 
      ELKEGLRAS HTAPPVLFS HREPPRELQ NTDAKVDNN 
      VGYVTFVLF PRHTARTTR DNTIDLIHM FRHYLHYHI 
      KCSKAYIHS RMRAKTSDF LKVLNRARP ENKSTDKKT 
      ITGRTFVRR D
    CDS sequence
    • >TC034118 RA
      atgattttg cttgagaca aacaatcgg gtagttgaa 
      gagacatta accgtgaag ataaaaagt gcccaagcc 
      gggttaaaa aacgagtca atcgacata acagtatcg 
      gacttcgac ggtgttttg taccacatt tcaaaccta 
      aacggagac aaaaccaaa attaggata agcatttcc 
      ctcaaattc tacaaagaa ctgcaagag cacggcgcc 
      gaccagctc ctcaagcgc gagtacggc ccccttctg 
      gccgcccct gaagacggc tacaacgtt tcgatccta 
      ttcgacctt gagaacatc ccgactgat tggccccaa 
      gtcatcaaa aaaatcggg cttttgaag cgcaactgc 
      ttcgcttca gtgtttgag aaatacttc gattttcaa 
      gaaaacgga gacgaggga cagaaacgt gcggtgatt 
      cactaccgc gatgacgaa acaatgtac gttgaggcg 
      aaggcggac cgagtcacc gtcgtcttt tccacgcgt 
      tttcgggac gaagacgac gtggtcatc gggaaggtg 
      ttcatgcag gagctcaag gaggggctc agggcgtcg 
      cacacggcg ccccccgtg ctcttcagc caccgggag 
      cccccccgg gagctccag aacaccgac gcgaaggtc 
      gataacaac gtggggtac gtcactttt gtcctgttc 
      ccgagacac acggcaagg accacgagg gacaacaca 
      atagattta attcacatg tttcgccat tatctacat 
      tatcacatc aaatgctcc aaggcctac attcacagt 
      cggatgagg gccaagacg tccgatttc ctcaaggtc 
      ctgaacagg gccaggccg gagaacaag tcgacagat 
      aagaaaact ataacgtac cgcaactgc ttcgcttca 
      gtgtttgag aaatacttc gattttcaa gaaaacgga 
      gacgaggga cagaaacgt gcggtgatt cactaccgc 
      gatgacgaa acaatgtac gttgaggcg aaggcggac 
      cgagtcacc gtcgtcttt tccacgcgt tttcgggac 
      gaagacgac gtggtcatc gggaaggtg ttcatgcag 
      gagctcaag gaggggctc agggcgtcg cacacggcg 
      ccccccgtg ctcttcagc caccgggag cccccccgg 
      gagctccag aacaccgac gcgaaggtc gataacaac 
      gtggggtac gtcactttt gtcctgttc ccgagacac 
      acggcaagg accacgagg gacaacaca atagattta 
      attcacatg tttcgccat tatctacat tatcacatc 
      aaatgctcc aaggcctac attcacagt cggatgagg 
      gccaagacg tccgatttc ctcaaggtc ctgaacagg 
      gccaggccg gagaacaag tcgacagat aagaaaact 
      ataacaggg agaactttc gttcgacgg gattga
    mRNA sequence
    • >TC034118 RA
      agtcagcca cagcacctc ccatgagca gtcagacct 
      agtaaatgt attttttta gttaaaaac cacaattcc 
      caatcattt aaaatgatt ttgcttgag acaaacaat 
      cgggtagtt gaagagaca ttaaccgtg aagataaaa 
      agtgcccaa gccgggtta aaaaacgag tcaatcgac 
      ataacagta tcggacttc gacggtgtt ttgtaccac 
      atttcaaac ctaaacgga gacaaaacc aaaattagg 
      ataagcatt tccctcaaa ttctacaaa gaactgcaa 
      gagcacggc gccgaccag ctcctcaag cgcgagtac 
      ggccccctt ctggccgcc cctgaagac ggctacaac 
      gtttcgatc ctattcgac cttgagaac atcccgact 
      gattggccc caagtcatc aaaaaaatc gggcttttg 
      aagcgcaac tgcttcgct tcagtgttt gagaaatac 
      ttcgatttt caagaaaac ggagacgag ggacagaaa 
      cgtgcggtg attcactac cgcgatgac gaaacaatg 
      tacgttgag gcgaaggcg gaccgagtc accgtcgtc 
      ttttccacg cgttttcgg gacgaagac gacgtggtc 
      atcgggaag gtgttcatg caggagctc aaggagggg 
      ctcagggcg tcgcacacg gcgcccccc gtgctcttc 
      agccaccgg gagcccccc cgggagctc cagaacacc 
      gacgcgaag gtcgataac aacgtgggg tacgtcact 
      tttgtcctg ttcccgaga cacacggca aggaccacg 
      agggacaac acaatagat ttaattcac atgtttcgc 
      cattatcta cattatcac atcaaatgc tccaaggcc 
      tacattcac agtcggatg agggccaag acgtccgat 
      ttcctcaag gtcctgaac agggccagg ccggagaac 
      aagtcgaca gataagaaa actataacg taccgcaac 
      tgcttcgct tcagtgttt gagaaatac ttcgatttt 
      caagaaaac ggagacgag ggacagaaa cgtgcggtg 
      attcactac cgcgatgac gaaacaatg tacgttgag 
      gcgaaggcg gaccgagtc accgtcgtc ttttccacg 
      cgttttcgg gacgaagac gacgtggtc atcgggaag 
      gtgttcatg caggagctc aaggagggg ctcagggcg 
      tcgcacacg gcgcccccc gtgctcttc agccaccgg 
      gagcccccc cgggagctc cagaacacc gacgcgaag 
      gtcgataac aacgtgggg tacgtcact tttgtcctg 
      ttcccgaga cacacggca aggaccacg agggacaac 
      acaatagat ttaattcac atgtttcgc cattatcta 
      cattatcac atcaaatgc tccaaggcc tacattcac 
      agtcggatg agggccaag acgtccgat ttcctcaag 
      gtcctgaac agggccagg ccggagaac aagtcgaca 
      gataagaaa actataaca gggagaact ttcgttcga 
      cgggattga tgtgtatag tactagtga actttgctt 
      gtaagatca gctcaaaaa aggactaat aactgccga 
      caatttatt aaacttaac tgtttcatt caattttcc 
      catcagtca gttcttgca cagcctctc ttgcagttt 
      atttattaa gtcaatcaa ttggttcat tcagtactg 
      aatgctaca gttcagtta atagaagtg ctgattttt 
      cagctactt gtgtacata gattcaatc ttttaacca 
      tgtgcttcc tttgaaata tgggtaagc acatcaata 
      ttgtgaatt atatatttc taatacaaa gtttaatta 
      catattttt tacccagtt tgcgagacg tgttttagg 
      ttttttact atgtatccg cttgttgtt atcattttg 
      attcggaac gatcctttt tactattga gaacttgaa 
      ccgccaaat aaattgtat aacttaaca tagtttaat 
      aggtaataa atgaatcgt caatttcta tagcaggtt 
      gcaatattt tcggatttc tgtaggcaa ttggacgca 
      ttcttcatg ctcatttca tttttatat tacattaac 
      agagcaaga cactgttta attcaataa tagaaaata 
      aaatgttat tttaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY