| General information | |||
| TC006530 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11051063..11053239 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006530 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01058 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
- >TC006530 RA
MSTQPKTNL LDYISSLDI TAKPKYLRL TGIVCTIGP ACQKVEILE KMLEAGMNV ARLNFSHGT HEYHVDLIK NIRTAVDNY SKRIGRYCP LAIAIDTKG SEIRTGVLE GGISAEVVL EKGSKVTVT TDEKYAKKC TKSVIYVDY KNITKVQKP GNQIFIDDG LITLNCEKI QGSEITCSV ANGGKLGSS KGVNLPGVE KDLPTVSEK DKKDLKFAV EQNVDCVFA SFTRNAEEV RQIRSILGD SNIRVIAKI ENTQGVKNM DEIIEAADG ILIDRGDLG MEISFQKVF LAQKAIIAR CNKVGKPII IATHLLESM VDKPRPTRA ESSDVANAV LDGADCVML AGETAKGLY PVECVETMA LICKEAEAA VWQKQLFND LKSQPKVPL DIVQTTAIS TVEASMNSL ATAIIVVTK TGKSAQLLS KYKPKCPII AVTRNAQVA RQLHLFRAI MPLYFDGLC DFKGEGDCY VWVLQAKAQ PSG
- >TC006530 RB
MSTQPKTNL LDYISSLDI TAKPKYLRL TGIVCTIGP ACQKVEILE KMLEAGMNV ARLNFSHGT HEYHVDLIK NIRTAVDNY SKRIGRYCP LAIAIDTKG SEIRTGVLE GGISAEVVL EKGSKVTVT TDEKYAKKC TKSVIYVDY KNITKVQKP GNQIFIDDG LITLNCEKI QGSEITCSV ANGGKLGSS KGVNLPGVE KDLPTVSEK DKKDLKFAV EQNVDCVFA SFTRNAEEV RQIRSILGD SNIRVIAKI ENTQGVKNM DEIIEAADG ILIDRGDLG MEISFQKVF LAQKAIIAR CNKVGKPII IATHLLESM VDKPRPTRA ESSDVANAV LDGADCVML AGETAKGLY PVECVETMA LICKEAEAA VWQKQLFND LKSQPKVPL DIVQTTAIS TVEASMNSL ATAIIVVTK TGKSAQLLS KYKPKCPII AVTRNAQVA RQLHLFRAI MPLYFDGQS PTEWLKNVE VRVKAGVSY GKSLGFVKS GDTVIIVTG LHQGPGSTN TLTLMTVQ
- >TC006530 RA
atgagtacg cagccgaaa acaaacctc ctggactac atcagtagt ttggacatt acagccaaa ccgaaatat ctgcggcta acgggaatc gtgtgtacg atcggacca gcatgccag aaggtagag attttggag aaaatgctc gaagctgga atgaacgtg gcacgtttg aacttctct cacggtact cacgagtac cacgtcgat ttgatcaaa aacataagg accgctgtg gacaactac agcaagaga ataggccgc tactgcccc cttgccatc gccatcgac accaaaggc tccgaaatc cgaactgga gtactagaa gggggcatc tctgccgaa gtggtcctg gagaaaggg tcaaaagtg accgtgaca accgacgaa aagtatgcc aaaaagtgc accaagagc gtcatctac gtcgattat aaaaacatc accaaggtg cagaaaccc ggaaaccag attttcatc gacgacggt ttgattacc ctcaactgt gagaaaatc caaggctcg gagatcact tgctcggtt gccaatggg ggaaaactg gggagtagt aaaggggtg aacctgcca ggcgtcgag aaagacctg cccacggtt tcggaaaag gacaagaag gatttgaaa ttcgcagtt gagcagaac gtcgactgt gttttcgcc tcgttcaca cgaaacgcc gaagaagtg cgccagatt cgctccatt ttaggcgac agcaacatt cgggtgatt gccaagatc gagaacacg caaggtgtg aagaacatg gacgagatt attgaagct gccgatggg attctcatc gatcggggc gacctgggg atggaaatc tccttccag aaggtgttc ctcgcccag aaagccatc atcgcgagg tgtaacaag gttggaaag cccattatt atcgccacc catttgctg gaatcaatg gttgataaa ccaaggcct acgagagcc gagagctcg gatgtggcc aatgctgtg ctggacggt gccgactgt gtgatgcta gcaggggag acggccaag ggcttgtac cctgtggag tgtgtggag accatggct ttgatttgc aaagaggcc gaggcagcg gtgtggcaa aaacagctc tttaacgac ctcaaaagc cagcctaaa gtaccgctt gacatcgtc caaactacc gcaatatcc acagttgaa gcctcgatg aactcgcta gccacggcc atcatcgtg gtcacgaaa acgggaaaa tcggcacaa ttgttgtca aaatacaag ccaaagtgc ccgattatt gcagtgacg aggaatgcg caagttgcg cgccagtta cacttgttc agggcgatt atgcccttg tattttgac ggtttgtgt gattttaag ggtgagggt gattgttac gtgtgggtt ttgcaggcc aaagcccaa ccgagtggt tga
- >TC006530 RB
atgagtacg cagccgaaa acaaacctc ctggactac atcagtagt ttggacatt acagccaaa ccgaaatat ctgcggcta acgggaatc gtgtgtacg atcggacca gcatgccag aaggtagag attttggag aaaatgctc gaagctgga atgaacgtg gcacgtttg aacttctct cacggtact cacgagtac cacgtcgat ttgatcaaa aacataagg accgctgtg gacaactac agcaagaga ataggccgc tactgcccc cttgccatc gccatcgac accaaaggc tccgaaatc cgaactgga gtactagaa gggggcatc tctgccgaa gtggtcctg gagaaaggg tcaaaagtg accgtgaca accgacgaa aagtatgcc aaaaagtgc accaagagc gtcatctac gtcgattat aaaaacatc accaaggtg cagaaaccc ggaaaccag attttcatc gacgacggt ttgattacc ctcaactgt gagaaaatc caaggctcg gagatcact tgctcggtt gccaatggg ggaaaactg gggagtagt aaaggggtg aacctgcca ggcgtcgag aaagacctg cccacggtt tcggaaaag gacaagaag gatttgaaa ttcgcagtt gagcagaac gtcgactgt gttttcgcc tcgttcaca cgaaacgcc gaagaagtg cgccagatt cgctccatt ttaggcgac agcaacatt cgggtgatt gccaagatc gagaacacg caaggtgtg aagaacatg gacgagatt attgaagct gccgatggg attctcatc gatcggggc gacctgggg atggaaatc tccttccag aaggtgttc ctcgcccag aaagccatc atcgcgagg tgtaacaag gttggaaag cccattatt atcgccacc catttgctg gaatcaatg gttgataaa ccaaggcct acgagagcc gagagctcg gatgtggcc aatgctgtg ctggacggt gccgactgt gtgatgcta gcaggggag acggccaag ggcttgtac cctgtggag tgtgtggag accatggct ttgatttgc aaagaggcc gaggcagcg gtgtggcaa aaacagctc tttaacgac ctcaaaagc cagcctaaa gtaccgctt gacatcgtc caaactacc gcaatatcc acagttgaa gcctcgatg aactcgcta gccacggcc atcatcgtg gtcacgaaa acgggaaaa tcggcacaa ttgttgtca aaatacaag ccaaagtgc ccgattatt gcagtgacg aggaatgcg caagttgcg cgccagtta cacttgttc agggcgatt atgcccttg tattttgac ggccaaagc ccaaccgag tggttgaaa aatgtggaa gtccgggtt aaagccgga gttagttat ggaaagtcg ttggggttt gttaaatca ggggatact gtaattatt gtgacgggt ttgcatcaa gggcctggc tctacaaac actcttacg ttgatgact gtccaatga
- >TC006530 RA
gataagaaa acacaaata ttaaaataa gcaaacgtg aaacgactc cacagcgtt tgttattcc agtcgcaat cacgccgag tgatttttg tacgaactg ataatcagt aagcacact caatgatcc ccactatca gctcctctt acataaaca tctctatca cgcatcgtt tcccaatat catcccaac ttgtgtgaa agtcaaaac gaacaagac ctcaaacca tttagttag tcaaaatga gtacgcagc cgaaaacaa acctcctgg actacatca gtagtttgg acattacag ccaaaccga aatatctgc ggctaacgg gaatcgtgt gtacgatcg gaccagcat gccagaagg tagagattt tggagaaaa tgctcgaag ctggaatga acgtggcac gtttgaact tctctcacg gtactcacg agtaccacg tcgatttga tcaaaaaca taaggaccg ctgtggaca actacagca agagaatag gccgctact gcccccttg ccatcgcca tcgacacca aaggctccg aaatccgaa ctggagtac tagaagggg gcatctctg ccgaagtgg tcctggaga aagggtcaa aagtgaccg tgacaaccg acgaaaagt atgccaaaa agtgcacca agagcgtca tctacgtcg attataaaa acatcacca aggtgcaga aacccggaa accagattt tcatcgacg acggtttga ttaccctca actgtgaga aaatccaag gctcggaga tcacttgct cggttgcca atgggggaa aactgggga gtagtaaag gggtgaacc tgccaggcg tcgagaaag acctgccca cggtttcgg aaaaggaca agaaggatt tgaaattcg cagttgagc agaacgtcg actgtgttt tcgcctcgt tcacacgaa acgccgaag aagtgcgcc agattcgct ccattttag gcgacagca acattcggg tgattgcca agatcgaga acacgcaag gtgtgaaga acatggacg agattattg aagctgccg atgggattc tcatcgatc ggggcgacc tggggatgg aaatctcct tccagaagg tgttcctcg cccagaaag ccatcatcg cgaggtgta acaaggttg gaaagccca ttattatcg ccacccatt tgctggaat caatggttg ataaaccaa ggcctacga gagccgaga gctcggatg tggccaatg ctgtgctgg acggtgccg actgtgtga tgctagcag gggagacgg ccaagggct tgtaccctg tggagtgtg tggagacca tggctttga tttgcaaag aggccgagg cagcggtgt ggcaaaaac agctcttta acgacctca aaagccagc ctaaagtac cgcttgaca tcgtccaaa ctaccgcaa tatccacag ttgaagcct cgatgaact cgctagcca cggccatca tcgtggtca cgaaaacgg gaaaatcgg cacaattgt tgtcaaaat acaagccaa agtgcccga ttattgcag tgacgagga atgcgcaag ttgcgcgcc agttacact tgttcaggg cgattatgc ccttgtatt ttgacggtt tgtgtgatt ttaagggtg agggtgatt gttacgtgt gggttttgc aggccaaag cccaaccga gtggttgaa aaatgtgga agtccgggt taaagccgg agttagtta tggaaagtc gttggggtt tgttaaatc aggggatac tgtaattat tgtgacggg tttgcatca agggcctgg ctctacaaa cactcttac gttgatgtg agttggcag aaatgtgtg tttaattat taaatgttt gtttacagg actgtccaa tgaggttga gtgttttgg gtttagtat tgtgttgct aattaaaac tgtaataat gttgttaat ttaaaacgt tgttttttt aataattct gcttattaa agctagtga ttattgacg attgtccct aattgtaac ataacatgc gtaataaag taattgtat cgat
- >TC006530 RB
gataagaaa acacaaata ttaaaataa gcaaacgtg aaacgactc cacagcgtt tgttattcc agtcgcaat cacgccgag tgatttttg tacgaactg ataatcagt aagcacact caatgatcc ccactatca gctcctctt acataaaca tctctatca cgcatcgtt tcccaatat catcccaac ttgtgtgaa agtcaaaac gaacaagac ctcaaacca tttagttag tcaaaatga gtacgcagc cgaaaacaa acctcctgg actacatca gtagtttgg acattacag ccaaaccga aatatctgc ggctaacgg gaatcgtgt gtacgatcg gaccagcat gccagaagg tagagattt tggagaaaa tgctcgaag ctggaatga acgtggcac gtttgaact tctctcacg gtactcacg agtaccacg tcgatttga tcaaaaaca taaggaccg ctgtggaca actacagca agagaatag gccgctact gcccccttg ccatcgcca tcgacacca aaggctccg aaatccgaa ctggagtac tagaagggg gcatctctg ccgaagtgg tcctggaga aagggtcaa aagtgaccg tgacaaccg acgaaaagt atgccaaaa agtgcacca agagcgtca tctacgtcg attataaaa acatcacca aggtgcaga aacccggaa accagattt tcatcgacg acggtttga ttaccctca actgtgaga aaatccaag gctcggaga tcacttgct cggttgcca atgggggaa aactgggga gtagtaaag gggtgaacc tgccaggcg tcgagaaag acctgccca cggtttcgg aaaaggaca agaaggatt tgaaattcg cagttgagc agaacgtcg actgtgttt tcgcctcgt tcacacgaa acgccgaag aagtgcgcc agattcgct ccattttag gcgacagca acattcggg tgattgcca agatcgaga acacgcaag gtgtgaaga acatggacg agattattg aagctgccg atgggattc tcatcgatc ggggcgacc tggggatgg aaatctcct tccagaagg tgttcctcg cccagaaag ccatcatcg cgaggtgta acaaggttg gaaagccca ttattatcg ccacccatt tgctggaat caatggttg ataaaccaa ggcctacga gagccgaga gctcggatg tggccaatg ctgtgctgg acggtgccg actgtgtga tgctagcag gggagacgg ccaagggct tgtaccctg tggagtgtg tggagacca tggctttga tttgcaaag aggccgagg cagcggtgt ggcaaaaac agctcttta acgacctca aaagccagc ctaaagtac cgcttgaca tcgtccaaa ctaccgcaa tatccacag ttgaagcct cgatgaact cgctagcca cggccatca tcgtggtca cgaaaacgg gaaaatcgg cacaattgt tgtcaaaat acaagccaa agtgcccga ttattgcag tgacgagga atgcgcaag ttgcgcgcc agttacact tgttcaggg cgattatgc ccttgtatt ttgacggcc aaagcccaa ccgagtggt tgaaaaatg tggaagtcc gggttaaag ccggagtta gttatggaa agtcgttgg ggtttgtta aatcagggg atactgtaa ttattgtga cgggtttgc atcaagggc ctggctcta caaacactc ttacgttga tgactgtcc aatgaggtt gagtgtttt gggtttagt attgtgttg ctaattaaa actgtaata atgttgtta atttaaaac gttgttttt ttaataatt ctgcttatt aaagctagt gattattga cgattgtcc ctaattgta acataacat gcgtaataa agtaattgt atcgat
