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Gene information
General information
TC006517 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10882985..10885839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006517
    iBeetle screen iB_01057

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006517 RA
      MKSPPSLYA ICVKAAVKD CVTVCKFCK KEFRSLPDN 
      VLFDFYYTM FTEKRACLL AVEFSELEV FIRMLNVKH 
      KRVKLLKSF QGLINHGSN VPDELIQDF SNYLQKEPL 
      RAISVGIHV GSFFNEGGW YDYSIKILT LTEDLCKEQ 
      DESVEILKK CLECYHKRI YAETIYCEF KAAENTFNS 
      AQKVIKQLE KLDALPNLA GLYANFSTL YFHRSEYDE 
      SFAWSKKAL VLLNNKLPS RIIIEVLRQ ASRACVVKR 
      RFNQAGLLI RQAVNLAQE IYERDGHPH FSDTLLDYG 
      FYLLNFDSI QESVKVYER SLQIRMDVF EKNNIHLAL 
      AHEDMAYAL YVNEYSSGR FYAARENAD RSIRLMERI 
      LPNNHLMLA SAKRVKALI LEEIALDMR EGNNSHLQE 
      KYLNEAEEL HRTALELSG KAFGEKNVQ TAKHYGNLG 
      RLYQSMRQY EQAEKMHLK AIAIKEELL GSADYEVGL 
      SIGHLASLY NYHMKRHHD AEKLYMRSM EINLNLFGE 
      TYSGLEYDY RGLINVYSK LCDQSNVVL YTLKMRDWS 
      LLRAQSKPP AHVEEILPL KEIIEKFSR MC
    CDS sequence
    • >TC006517 RA
      atgaaaagt cctccgtcg ctatacgca atttgtgtc 
      aaagcagct gtcaaagat tgtgttaca gtttgtaaa 
      ttttgcaaa aaagagttc agatctttg cctgataat 
      gtcctcttt gatttttat tacacaatg ttcactgaa 
      aagcgggcg tgccttctc gccgtcgaa ttcagtgaa 
      ctggaagtc tttattcga atgcttaac gttaaacac 
      aaacgcgtc aagttgctg aagagtttt caaggctta 
      attaatcac ggatcgaac gttcctgac gaactaatc 
      caggacttt tcaaattac ttgcaaaag gagcccttg 
      cgggcgata agtgtaggc atacacgtg ggcagcttc 
      ttcaacgaa gggggctgg tatgactac agcattaag 
      atactgaca ctaacagag gacttgtgc aaggaacag 
      gacgaaagc gtcgagatc ctgaagaaa tgcctcgag 
      tgctatcac aagcgaatt tacgcggag accatttac 
      tgcgagttc aaagcggcc gaaaacacg ttcaattcc 
      gcgcaaaaa gtaataaaa cagttggaa aaattggac 
      gctttacct aatctagct ggcctctat gcgaatttc 
      tcaacgctg tactttcat cgtagcgaa tatgatgag 
      tcgtttgcg tggtctaaa aaagcacta gtcttactt 
      aataacaaa cttccctca agaatcatc attgaggtg 
      ctgagacag gcgtcaaga gcttgcgtc gtcaaacgg 
      cgcttcaat caagcgggc cttctgata agacaagcg 
      gttaacttg gcgcaagaa atttacgag cgtgacggc 
      cacccccat ttctcggac acgctgctt gattacggc 
      ttttattta ctcaatttt gactcaatc caagagagc 
      gtcaaggtg tacgaacgg tctctgcaa atcagaatg 
      gacgttttc gagaaaaat aatatacac ctggctctc 
      gcacacgaa gacatggcc tacgctctc tacgtcaac 
      gagtacagc tcggggagg ttctatgcc gcaagggaa 
      aatgcggac cgttcgatt cgcctcatg gagcgcatt 
      ctccccaac aaccactta atgctggcg tcggccaag 
      cgcgtaaag gcgctcatt ctcgaagaa atagcgttg 
      gatatgcgc gagggcaac aactcgcat ttgcaggag 
      aaatacctg aatgaggct gaagagctg caccggacg 
      gcgttagaa ctgtctggc aaagccttc ggcgagaaa 
      aacgtccaa acggcgaag cattacggc aacttgggg 
      cgcttatac caaagtatg cgccagtac gagcaagcg 
      gagaaaatg cacttgaag gcgattgcc attaaggaa 
      gagttgctc ggaagcgcc gattacgag gtggggctc 
      agtatcggg cacctggcc tcgctgtac aactaccac 
      atgaagcgc caccacgac gccgaaaag ctgtacatg 
      cgctcgatg gagataaac ctgaatttg ttcggggag 
      acgtattca ggtttggag tacgattat cggggcttg 
      atcaacgtt tactcgaaa ctgtgtgat cagtccaac 
      gttgtttta tatactttg aaaatgcgc gactggagt 
      ttgctgcgg gcgcagagc aaaccgccg gctcatgtt 
      gaagaaatt ttaccgctc aaggaaatt atcgaaaag 
      ttctcgcgt atgtgctga
    mRNA sequence
    • >TC006517 RA
      ctggtcaat gttatttgg agttatatc agctgcttc 
      attaatgtg tgattagca aataaaaac tagttcaat 
      attgtgtgc aatcgtggt aaaaatgaa aagtcctcc 
      gtcgctata cgcaatttg tgtcaaagc agctgtcaa 
      agattgtgt tacagtttg taaattttg caaaaaaga 
      gttcagatc tttgcctga taatgtcct ctttgattt 
      ttattacac aatgttcac tgaaaagcg ggcgtgcct 
      tctcgccgt cgaattcag tgaactgga agtctttat 
      tcgaatgct taacgttaa acacaaacg cgtcaagtt 
      gctgaagag ttttcaagg cttaattaa tcacggatc 
      gaacgttcc tgacgaact aatccagga cttttcaaa 
      ttacttgca aaaggagcc cttgcgggc gataagtgt 
      aggcataca cgtgggcag cttcttcaa cgaaggggg 
      ctggtatga ctacagcat taagatact gacactaac 
      agaggactt gtgcaagga acaggacga aagcgtcga 
      gatcctgaa gaaatgcct cgagtgcta tcacaagcg 
      aatttacgc ggagaccat ttactgcga gttcaaagc 
      ggccgaaaa cacgttcaa ttccgcgca aaaagtaat 
      aaaacagtt ggaaaaatt ggacgcttt acctaatct 
      agctggcct ctatgcgaa tttctcaac gctgtactt 
      tcatcgtag cgaatatga tgagtcgtt tgcgtggtc 
      taaaaaagc actagtctt acttaataa caaacttcc 
      ctcaagaat catcattga ggtgctgag acaggcgtc 
      aagagcttg cgtcgtcaa acggcgctt caatcaagc 
      gggccttct gataagaca agcggttaa cttggcgca 
      agaaattta cgagcgtga cggccaccc ccatttctc 
      ggacacgct gcttgatta cggctttta tttactcaa 
      ttttgactc aatccaaga gagcgtcaa ggtgtacga 
      acggtctct gcaaatcag aatggacgt tttcgagaa 
      aaataatat acacctggc tctcgcaca cgaagacat 
      ggcctacgc tctctacgt caacgagta cagctcggg 
      gaggttcta tgccgcaag ggaaaatgc ggaccgttc 
      gattcgcct catggagcg cattctccc caacaacca 
      cttaatgct ggcgtcggc caagcgcgt aaaggcgct 
      cattctcga agaaatagc gttggatat gcgcgaggg 
      caacaactc gcatttgca ggagaaata cctgaatga 
      ggctgaaga gctgcaccg gacggcgtt agaactgtc 
      tggcaaagc cttcggcga gaaaaacgt ccaaacggc 
      gaagcatta cggcaactt ggggcgctt ataccaaag 
      tatgcgcca gtacgagca agcggagaa aatgcactt 
      gaaggcgat tgccattaa ggaagagtt gctcggaag 
      cgccgatta cgaggtggg gctcagtat cgggcacct 
      ggcctcgct gtacaacta ccacatgaa gcgccacca 
      cgacgccga aaagctgta catgcgctc gatggagat 
      aaacctgaa tttgttcgg ggagacgta ttcaggttt 
      ggagtacga ttatcgggg cttgatcaa cgtttactc 
      gaaactgtg tgatcagtc caacgttgt tttatatac 
      tttgaaaat gcgcgactg gagtttgct gcgggcgca 
      gagcaaacc gccggctca tgttgaaga aattttacc 
      gctcaagga aattatcga aaagttctc gcgtatgtg 
      ctgaagtct agtcactag tgtaaacga aattaaaca 
      atgctgcca atctgttac tattgtccc atttttttt 
      attgttatg tttttattt ttgcgactc ggcagaatt 
      cgctgcttt aaaaatctg tgatataac taggacatt 
      cccgacgtg aatctcaca ctaaacgca tcgtggttt 
      ttcagttgc attactact tttgacgtg cttttattt 
      ccagtatta agttttgat cggtgtttt taatcttgg 
      gacatgtta cttccgtaa gagcaaaat ataaggcta 
      attgtagat ggggggcgt agccacagg cagttgaac 
      acgtggatg attgttatt gggtgatat cgcacgccg 
      cctccttga ttgtaaagc tatgttagt gtcgcatta 
      ttatatgac ttagaatta actcatcat tgattctca 
      tattaatca tatttttat tttgttatt agattgtga 
      ttcatattt tttacaact aattttagg agttatatc 
      cttttgtta cgcaacaat tatat
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