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Gene information
General information
TC006490 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10467450..10469440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006490
    iBeetle screen iB_01055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 head musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature potentially mostlynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    antenna not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006490 RA
      MNGDGDSES WLSGWEQQC ADSVDEQPD 
      FEQSLRDES ENSHTRIWT AFQDSAAAI 
      AQLYRDRVT GEPATLWLQ FQTAAGTVT 
      SLYKESSEG LRKSSDLAK QCGYQKRNK 
      EILNWAKRK RRLICREDL LAYLSGKLP 
      PKPNHHQYH HHNHHRNIS PPPGVAPPE 
      SLGLNSELH TFREALARS PLSCIARGP 
      DLCAFITGE MARHCKRPA SPSDVTMDS 
      PTQQKRPRY M
    CDS sequence
    • >TC006490 RA
      atgaacggt gacggtgat agtgagtct tggttatcg 
      ggatgggaa cagcagtgt gccgattcg gtggacgag 
      cagcccgac ttcgagcag tcccttcgt gacgaatcg 
      gagaattct cacacgcga atatggact gcgttccag 
      gactccgcc gcagccata gcccagctg tatcgcgat 
      cgggttaca ggagagccg gcgacgctc tggttgcag 
      ttccagacg gctgcgggc acggtcacc tcattatat 
      aaagaatct tcagagggg ctgcggaag tcgagcgac 
      ctggcgaaa cagtgcgga taccaaaaa cggaacaag 
      gaaatactc aattgggcc aagcggaaa cggcgcctg 
      atctgcaga gaggacctg ctcgcctac ctctccgga 
      aagcttcca cccaaaccc aaccaccac cagtaccac 
      caccacaac caccacagg aacatatcg ccgcctcct 
      ggagtcgcg ccccctgaa tctctgggt cttaatagt 
      gagttacac accttcagg gaggccttg gcgcgatcg 
      cccttgtct tgcattgcc cggggccca gacttgtgc 
      gcttttatc acaggggaa atggccagg cattgtaaa 
      cggccggcg agtccttcc gatgtgaca atggacagt 
      cctacacaa caaaaaaga cctcgatat atgtga
    mRNA sequence
    • >TC006490 RA
      agcgtttta ttccgagtt tccgttaat tttttctcc 
      tctcgttgg tgccccaat cgcagtgtc gccaccaca 
      cgagtccca tctgtcact tcgtcaaac atggccgtg 
      tgtagctga aagttatcg cgtagtttt ccgaaattc 
      cattgtgtt ccgttaatt tttggtcac aaaaacgtg 
      agatgaacg gtgacggtg atagtgagt cttggttat 
      cgggatggg aacagcagt gtgccgatt cggtggacg 
      agcagcccg acttcgagc agtcccttc gtgacgaat 
      cggagaatt ctcacacgc gaatatgga ctgcgttcc 
      aggactccg ccgcagcca tagcccagc tgtatcgcg 
      atcgggtta caggagagc cggcgacgc tctggttgc 
      agttccaga cggctgcgg gcacggtca cctcattat 
      ataaagaat cttcagagg ggctgcgga agtcgagcg 
      acctggcga aacagtgcg gataccaaa aacggaaca 
      aggaaatac tcaattggg ccaagcgga aacggcgcc 
      tgatctgca gagaggacc tgctcgcct acctctccg 
      gaaagcttc cacccaaac ccaaccacc accagtacc 
      accaccaca accaccaca ggaacatat cgccgcctc 
      ctggagtcg cgccccctg aatctctgg gtcttaata 
      gtgagttac acaccttca gggaggcct tggcgcgat 
      cgcccttgt cttgcattg cccggggcc cagacttgt 
      gcgctttta tcacagggg aaatggcca ggcattgta 
      aacggccgg cgagtcctt ccgatgtga caatggaca 
      gtcctacac aacaaaaaa gacctcgat atatgtgaa 
      aaacgtcat cagtcctgc agttttcct actcgttca 
      ataggtgca gttgccttc cgttttgta tacagtttt 
      tcatttgat ttttggtgc ttttaatgt ttttttaag 
      tctgtgata attgcctga acttattat tttcttctg 
      tttttaacg cggtaatat cggatttgg gggatagag 
      gtattggtc attctttta aattaattg ttttcgcta 
      caaaacgcg cgacgtgca ggaacgatg gggtaaata 
      tccatcact aaatattca atgaataaa gtagtttat 
      tattcatta gattgtaag gcaattaaa aataataca 
      aaaaattgg ccattacga aattgtaaa taagttctt 
      ctttcgtta aatacttgt tgaaaactt cttactgtt 
      tttttacac ttatgagac aaatcatta tattgtaat 
      atagaggtg cctcggtcg attttttat aatatgaag 
      ccttccaat tttttgaga atcgatata ttcaagaca 
      cagactgcc acttattat attttattt atttatatg 
      gagaggttg agattgttg atatcggga atttttttt 
      gaaaagata ccgagtctc aggatgcta aatacgatc 
      ttcacacac gactttata ttgatttat cttgtattc 
      caattgtta ttctgtata gttgcaaga ttcttattt 
      tcaacaagt gttaataga ctgtacgaa tatttgcac 
      atatttatt taattaaat ctagttttt taaaccttt 
      gttattttg tttcggtac ttctaagat ccaattatg 
      tttcgactt tttcagtga tgtaagtaa ttgttctgt 
      aaattatta ttggtctaa atatattat attggtcta 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY