Status
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Gene information
General information
TC006488 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10445557..10465236
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006488
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01054

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006488 RA
      MKRSNAVLE SMMNASTIL KAEDTPNSI TISLRTEPE 
      SAKEDQNPH TTEPTDLRY PPDSEPNSI PSPSIAPSF 
      GEGAMQIDD CPNQPADLS NKKPENLTC VPEIDFIKE 
      EVNDNASDY SNSSDPERL EVDMSQGVE EHSNSTTAS 
      ATSPVPDNV QPDEEASLW RALSHNGYN PNVPLSGEA 
      SQLLRKLIT CRKLGMSIT PAPPFVLDG RVSSPSEPL 
      NMETNPKPS TGRRKQSYP TKASIEDSR SPARDDGED 
      YIPDFTGNS PWCNLQIVK GGKGGAGMA RRVDLSCTN 
      CGTQTTTIW RRNMKGEMV CNACGLYYK LHGIDRPHT 
      MRRDTIHTR RRRPKAAEA RNEKDKCRE YPTINNQFK 
      APKKGHGNA ETADTEDML SALRRQIQP HLVMALQGH 
      KTTAYPHLQ AANFAHFLP TSGSPGGSA PEVESDEES 
      IADLPLNLV STQMTETES H
    • >TC006488 RB
      MCNVPPKFY QLCRLCLSV DKDGVGIFE DAGAHDDLP 
      AKIMTCLSI LVAKSDPLP HVICVRCIE QLEALYRFK 
      EVARKAEDL LNQFLTYTK QLAGTAEER MKRSNAVLE 
      SMMNASTIL KAEDTPNSI TISLRTEPE SAKEDQNPH 
      TTEPTDLRY PPDSEPNSI PSPSIAPSF GEGAMQIDD 
      CPNQPADLS NKKPENLTC VPEIDFIKE EVNDNASDY 
      SNSSDPERL EVDMSQGVE EHSNSTTAS ATSPVPDNV 
      QPDEEASLW RALSHNGYN PNVPLSGEA SQLLRKLIT 
      CRKLGMSIT PAPPFVLDG RVSSPSEPL NMETNPKPS 
      TGRRKQSYP TKASIEDSR SPARDDGED YIPDFTGNS 
      PWCNLQIVK GGKGGAGMA RRVDLSCTN CGTQTTTIW 
      RRNMKGEMV CNACGLYYK LHGIDRPHT MRRDTIHTR 
      RRRPKAAEA RNEKDKCRE YPTINNQFK APKKGHGNA 
      ETADTEDML SALRRQIQP HLVMALQGH KTTAYPHLQ 
      AANFAHFLP TSGSPGGSA PEVESDEES IADLPLNLV 
      STQMTETES H
    CDS sequence
    • >TC006488 RA
      atgaagcgc tcgaatgcc gttctggag tcgatgatg 
      aacgcctcc acaatccta aaagccgaa gatacaccc 
      aacagtatt acaatcagt ctgaggacg gaaccggag 
      tcggcgaaa gaggaccaa aacccgcac acaaccgaa 
      ccaacagac ttacgatat ccgccagat agcgaaccg 
      aacagtata ccctcgcca tcgatagca cccagcttc 
      ggcgagggg gcgatgcaa atcgacgac tgtcccaac 
      cagccggcc gacctatcg aacaagaaa ccggagaac 
      ctcacctgc gtgccggaa atcgatttc atcaaagag 
      gaggtgaac gacaacgcc tcggactac agcaacagc 
      tcggacccc gagcggctg gaggtggac atgtcgcag 
      ggcgtggag gagcattcc aattcgacg acggcgagc 
      gccacctcc cccgtcccc gacaacgtg cagcccgac 
      gaagaggcg tctttgtgg cgcgcgctg tcccacaac 
      ggctacaac cccaacgtg cccttgagc ggcgaagcc 
      agccaacta ctcaggaaa ctgataacg tgccgaaag 
      ctaggcatg agcataacc ccagcacct cccttcgtc 
      ctcgacggc cgagtcagc tcccccagc gaacccctc 
      aacatggaa accaacccc aagccgtcg acgggccgg 
      agaaaacag agttacccc accaaagcc tcgatcgag 
      gactccagg agtccggcc cgcgacgac ggcgaggac 
      tacatcccg gacttcacc ggaaacagc ccctggtgc 
      aacctccag atcgtgaaa gggggcaaa gggggcgcc 
      ggaatggcg cgacgggtc gatctctcg tgcacgaat 
      tgcgggacg caaaccacg accatctgg aggaggaac 
      atgaagggg gaaatggtc tgcaatgct tgtgggctc 
      tactacaag ctccacggc attgatagg ccgcatacg 
      atgcgaagg gacacgatc cacacgagg cgcagacgt 
      cccaaagcg gccgaagcg cggaacgag aaggacaag 
      tgccgggag tacccgact atcaataat cagtttaaa 
      gcgccgaag aaggggcac gggaacgcc gagaccgcc 
      gacactgaa gacatgttg agcgcgctg cggcgacag 
      atccagccc cacttggtg atggcgttg cagggacac 
      aaaaccacc gcctatcct cacttacaa gcagccaat 
      tttgcccat ttcttgccg acgagcggt tcgccgggg 
      ggctccgcc cccgaggtg gagtccgac gaggagagt 
      atagcggac ttgccacta aaccttgtc tcgactcaa 
      atgacggaa acggaaagt cattga
    • >TC006488 RB
      atgtgtaat gtaccgcca aagttttat cagctctgt 
      cgtctttgt ttatcggta gataaggat ggtgtcggg 
      attttcgag gatgcaggc gcccacgac gacctcccg 
      gccaagata atgacgtgt ctctccatc ctggttgcc 
      aagagcgac cctctgccg cacgtcatc tgtgtgaga 
      tgcatcgaa cagctcgaa gctctctac aggttcaaa 
      gaggtcgcc cggaaagcc gaagacctc ctcaaccaa 
      ttcctaacg tatacgaag cagctggcc ggaacagca 
      gaggagagg atgaagcgc tcgaatgcc gttctggag 
      tcgatgatg aacgcctcc acaatccta aaagccgaa 
      gatacaccc aacagtatt acaatcagt ctgaggacg 
      gaaccggag tcggcgaaa gaggaccaa aacccgcac 
      acaaccgaa ccaacagac ttacgatat ccgccagat 
      agcgaaccg aacagtata ccctcgcca tcgatagca 
      cccagcttc ggcgagggg gcgatgcaa atcgacgac 
      tgtcccaac cagccggcc gacctatcg aacaagaaa 
      ccggagaac ctcacctgc gtgccggaa atcgatttc 
      atcaaagag gaggtgaac gacaacgcc tcggactac 
      agcaacagc tcggacccc gagcggctg gaggtggac 
      atgtcgcag ggcgtggag gagcattcc aattcgacg 
      acggcgagc gccacctcc cccgtcccc gacaacgtg 
      cagcccgac gaagaggcg tctttgtgg cgcgcgctg 
      tcccacaac ggctacaac cccaacgtg cccttgagc 
      ggcgaagcc agccaacta ctcaggaaa ctgataacg 
      tgccgaaag ctaggcatg agcataacc ccagcacct 
      cccttcgtc ctcgacggc cgagtcagc tcccccagc 
      gaacccctc aacatggaa accaacccc aagccgtcg 
      acgggccgg agaaaacag agttacccc accaaagcc 
      tcgatcgag gactccagg agtccggcc cgcgacgac 
      ggcgaggac tacatcccg gacttcacc ggaaacagc 
      ccctggtgc aacctccag atcgtgaaa gggggcaaa 
      gggggcgcc ggaatggcg cgacgggtc gatctctcg 
      tgcacgaat tgcgggacg caaaccacg accatctgg 
      aggaggaac atgaagggg gaaatggtc tgcaatgct 
      tgtgggctc tactacaag ctccacggc attgatagg 
      ccgcatacg atgcgaagg gacacgatc cacacgagg 
      cgcagacgt cccaaagcg gccgaagcg cggaacgag 
      aaggacaag tgccgggag tacccgact atcaataat 
      cagtttaaa gcgccgaag aaggggcac gggaacgcc 
      gagaccgcc gacactgaa gacatgttg agcgcgctg 
      cggcgacag atccagccc cacttggtg atggcgttg 
      cagggacac aaaaccacc gcctatcct cacttacaa 
      gcagccaat tttgcccat ttcttgccg acgagcggt 
      tcgccgggg ggctccgcc cccgaggtg gagtccgac 
      gaggagagt atagcggac ttgccacta aaccttgtc 
      tcgactcaa atgacggaa acggaaagt cattga
    mRNA sequence
    • >TC006488 RA
      tgtgcgcaa aattaattg tgatttttt taggagagg 
      atgaagcgc tcgaatgcc gttctggag tcgatgatg 
      aacgcctcc acaatccta aaagccgaa gatacaccc 
      aacagtatt acaatcagt ctgaggacg gaaccggag 
      tcggcgaaa gaggaccaa aacccgcac acaaccgaa 
      ccaacagac ttacgatat ccgccagat agcgaaccg 
      aacagtata ccctcgcca tcgatagca cccagcttc 
      ggcgagggg gcgatgcaa atcgacgac tgtcccaac 
      cagccggcc gacctatcg aacaagaaa ccggagaac 
      ctcacctgc gtgccggaa atcgatttc atcaaagag 
      gaggtgaac gacaacgcc tcggactac agcaacagc 
      tcggacccc gagcggctg gaggtggac atgtcgcag 
      ggcgtggag gagcattcc aattcgacg acggcgagc 
      gccacctcc cccgtcccc gacaacgtg cagcccgac 
      gaagaggcg tctttgtgg cgcgcgctg tcccacaac 
      ggctacaac cccaacgtg cccttgagc ggcgaagcc 
      agccaacta ctcaggaaa ctgataacg tgccgaaag 
      ctaggcatg agcataacc ccagcacct cccttcgtc 
      ctcgacggc cgagtcagc tcccccagc gaacccctc 
      aacatggaa accaacccc aagccgtcg acgggccgg 
      agaaaacag agttacccc accaaagcc tcgatcgag 
      gactccagg agtccggcc cgcgacgac ggcgaggac 
      tacatcccg gacttcacc ggaaacagc ccctggtgc 
      aacctccag atcgtgaaa gggggcaaa gggggcgcc 
      ggaatggcg cgacgggtc gatctctcg tgcacgaat 
      tgcgggacg caaaccacg accatctgg aggaggaac 
      atgaagggg gaaatggtc tgcaatgct tgtgggctc 
      tactacaag ctccacggc attgatagg ccgcatacg 
      atgcgaagg gacacgatc cacacgagg cgcagacgt 
      cccaaagcg gccgaagcg cggaacgag aaggacaag 
      tgccgggag tacccgact atcaataat cagtttaaa 
      gcgccgaag aaggggcac gggaacgcc gagaccgcc 
      gacactgaa gacatgttg agcgcgctg cggcgacag 
      atccagccc cacttggtg atggcgttg cagggacac 
      aaaaccacc gcctatcct cacttacaa gcagccaat 
      tttgcccat ttcttgccg acgagcggt tcgccgggg 
      ggctccgcc cccgaggtg gagtccgac gaggagagt 
      atagcggac ttgccacta aaccttgtc tcgactcaa 
      atgacggaa acggaaagt cattgaaac gattaccag 
      tgataccaa ctacttaac tagatacgt acagaaaaa 
      gcttggttt attaagtgc acctaactc gattctttg 
      gttactagt ttaagtttt tgtttactt attacggag 
      ctctcttca tttttctct cgacccagt tttgtagat 
      atttattta ttttttatt ttattatga ttcagtact 
      tatttaatg acaaatact cagttctgt tacgttaac 
      ttaaattgt ctcttttca ccaaatgtg atatttttc 
      catgatgca ttattacca gtacgacac cgcaaccaa 
      attttttgc ttgaagcca gtagtacct tgtgtattt 
      tttagtttg taatcaata ctcagtatt ttaagagat 
      cttcacagt tatcgaaat ttgaccaaa atactcatt 
      atttattct cttattttg atgtgatgc aaataatct 
      taataataa tcaaatact ctttgatgg ttgattttt 
      cataatgaa acttaataa aatgttgta aaggag
    • >TC006488 RB
      atacaataa tgcaaaaac tgctataaa gcttttaaa 
      aataagatt ttttaaatt aatatttta ttatttgtt 
      ttagtgtaa acctttgat gtgctaaat tatgggttt 
      aaaactcac aatatcatt catgcactg ggttcaagt 
      tcaaatttt tttgcgtaa ttgtttaca cgttttttt 
      taaagaact gatcataaa aaaatttat aatattaat 
      aacttactt gtgaccaca aaaataaat taatcacgt 
      ctttatcaa ttttaattt ggtgtaatg tcagactga 
      aaaaaaccg ataaaacca tagattttc aaatcacgt 
      tagagaaat gatttttaa aaatgttta gagattttg 
      tgtggtcat gttggcagc gtgaaaact tgtcccagt 
      tgcgccttg taggttata caaccattt gaactggcg 
      cgtttctca gtctttggg tgaaggcag tgcttcagc 
      agccccact ttctggatt gaccactcg tccgaagac 
      cgtcgccac ctgtccgcc ggaggagta agctctgtg 
      tgggtcctc ctgccgtaa tcagcttac ctttttaat 
      caaggtggc catggcagt gccttaatt tctaagcga 
      gctcttgac aaacgtgaa atgtgctcg aggtgttgc 
      ggcaaagtc tctctcgca ccgaaaaat cactgaaaa 
      aaggctcaa caaaacccg cgaggctgt gaactagtt 
      cgaaattaa tgtttcatg tgtcggtgt gcttgatgt 
      gtccctttg tttttgtgt tggaagtgt tcgaatatg 
      tgtaatgta ccgccaaag ttttatcag ctctgtcgt 
      ctttgttta tcggtagat aaggatggt gtcgggatt 
      ttcgaggat gcaggcgcc cacgacgac ctcccggcc 
      aagataatg acgtgtctc tccatcctg gttgccaag 
      agcgaccct ctgccgcac gtcatctgt gtgagatgc 
      atcgaacag ctcgaagct ctctacagg ttcaaagag 
      gtcgcccgg aaagccgaa gacctcctc aaccaattc 
      ctaacgtat acgaagcag ctggccgga acagcagag 
      gagaggatg aagcgctcg aatgccgtt ctggagtcg 
      atgatgaac gcctccaca atcctaaaa gccgaagat 
      acacccaac agtattaca atcagtctg aggacggaa 
      ccggagtcg gcgaaagag gaccaaaac ccgcacaca 
      accgaacca acagactta cgatatccg ccagatagc 
      gaaccgaac agtataccc tcgccatcg atagcaccc 
      agcttcggc gagggggcg atgcaaatc gacgactgt 
      cccaaccag ccggccgac ctatcgaac aagaaaccg 
      gagaacctc acctgcgtg ccggaaatc gatttcatc 
      aaagaggag gtgaacgac aacgcctcg gactacagc 
      aacagctcg gaccccgag cggctggag gtggacatg 
      tcgcagggc gtggaggag cattccaat tcgacgacg 
      gcgagcgcc acctccccc gtccccgac aacgtgcag 
      cccgacgaa gaggcgtct ttgtggcgc gcgctgtcc 
      cacaacggc tacaacccc aacgtgccc ttgagcggc 
      gaagccagc caactactc aggaaactg ataacgtgc 
      cgaaagcta ggcatgagc ataacccca gcacctccc 
      ttcgtcctc gacggccga gtcagctcc cccagcgaa 
      cccctcaac atggaaacc aaccccaag ccgtcgacg 
      ggccggaga aaacagagt taccccacc aaagcctcg 
      atcgaggac tccaggagt ccggcccgc gacgacggc 
      gaggactac atcccggac ttcaccgga aacagcccc 
      tggtgcaac ctccagatc gtgaaaggg ggcaaaggg 
      ggcgccgga atggcgcga cgggtcgat ctctcgtgc 
      acgaattgc gggacgcaa accacgacc atctggagg 
      aggaacatg aagggggaa atggtctgc aatgcttgt 
      gggctctac tacaagctc cacggcatt gataggccg 
      catacgatg cgaagggac acgatccac acgaggcgc 
      agacgtccc aaagcggcc gaagcgcgg aacgagaag 
      gacaagtgc cgggagtac ccgactatc aataatcag 
      tttaaagcg ccgaagaag gggcacggg aacgccgag 
      accgccgac actgaagac atgttgagc gcgctgcgg 
      cgacagatc cagccccac ttggtgatg gcgttgcag 
      ggacacaaa accaccgcc tatcctcac ttacaagca 
      gccaatttt gcccatttc ttgccgacg agcggttcg 
      ccggggggc tccgccccc gaggtggag tccgacgag 
      gagagtata gcggacttg ccactaaac cttgtctcg 
      actcaaatg acggaaacg gaaagtcat tgaaacgat 
      taccagtga taccaacta cttaactag atacgtaca 
      gaaaaagct tggtttatt aagtgcacc taactcgat 
      tctttggtt actagttta agtttttgt ttacttatt 
      acggagctc tcttcattt ttctctcga cccagtttt 
      gtagatatt tatttattt tttatttta ttatgattc 
      agtacttat ttaatgaca aatactcag ttctgttac 
      gttaactta aattgtctc ttttcacca aatgtgata 
      tttttccat gatgcatta ttaccagta cgacaccgc 
      aaccaaatt ttttgcttg aagccagta gtaccttgt 
      gtatttttt agtttgtaa tcaatactc agtatttta 
      agagatctt cacagttat cgaaatttg accaaaata 
      ctcattatt tattctctt attttgatg tgatgcaaa 
      taatcttaa taataatca aatactctt tgatggttg 
      atttttcat aatgaaact taataaaat gttgtaaag 
      gag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY